Title:
Use of -80°C freezer / Rev No: / 1
Issue Date: / 08-Dec-2016
Page: / 1 of 2

Prepared by Approved by Approved Date Revision Date

Hsu Myat NoeDr. Chen Chia-Hung 08.12.2016 08.12.2019

1.0OBJECTIVE

This procedure states the proper use of department owned ultra-low temperature -80°C freezerat E3A level 7, National University of Singapore (NUS) and SINAPSE.

2.0SCOPE

This SOP is applicable to all lab users in Integrated Microfluidics Lab and SINAPSE.

3.0PROCEDURE

3.1 Sample preparation

  1. Ensure that all samples/boxes are labelled with contents, owner’s name and date.
  2. Know where you put your samples or where you are retrieving them from before you open the freezer door.

3.1 Using the equipment

  1. Open the outer door by pulling down the latch.
  2. Open the appropriate inner door and remove the storage box from which you will retrieve or deposit your sample. Work quickly as the temperature in the interior rises very quickly.
  3. Return the box to the same location in the freezer when finished.
  4. Check the door gasket and be sure that it is free of ice built up. Brush frost off doors to prevent ice built up.
  5. Quickly close all doors making sure outer latch is closed properly.
  6. Update the inventory list placed on the freezer door.

4.0 Training & Precautions:

  1. Wear lab coat and cryogloves when working at freezer. Low temperatures of -80°C can quickly cause frostbite and samples stored at these temperatures can damange the skin if handled with bare hands.
  2. When closing the outer door, be careful your fingers are not placed such that the latch of the door can crush them.
  3. Note that the freezer may vacuum down the door and it may take several minutes before the door can be re-opened.
  4. Place absorbent pads or cardboards on floor to soak up water from melting frost/ice, and place WET FLOOR sign to prevent injury to other people.
  5. Do not overload the power supply source and always keep the area surrounding the freezer dry to prevent short circuit. Clean up any spillage as soon as possible.

5.0 Emergency Contact

Yu Jiaqing (93964175), Dr. Chen Chia-Hung (98514596), Guo Song (84845239)

6.0References:

SOP from Department of Physiology Safety & Health, NUS.

Refer to manufacturer’s user manual for more information.