Feasibility Testing Report

Feasibility Testing Report

Test Title: RBC Suspension Using Prototype

Person in Charge: Ana Peredo & Chris Kirby

Helpers: Rest of team

Date: TBD

Questions:

●  Will vibration using one motor resuspend sedimented red blood cells in prototype tray?

●  What frequency will resuspend red blood cells after sedimentation has occurred?

●  Will vibration method keep already suspended red blood cells from settling for a longer period of time?

●  Does the suspension frequency change for maximum versus minimum fill volume?

●  How does different tube quantities affect RBC suspension?

●  How does location of the sample test tube on the tray affect RBC suspesion?

Assumptions:

●  Porcine blood will act similarly to human blood at room temperature.

●  Viscosity of the porcine blood closely matches the viscosity of the artificial blood that was prepared for the Blood Spilling report.

Materials:

●  Porcine Blood

●  Tray Prototype

●  5 mL test tubes

●  Hemocytometer

●  Optical Microscope

Testing Methods

RBC suspension will be tested as was tested using test fixture. Controls will be samples without vibration mixing and samples manually inverted before RBC count. RBC will be counted by pipetting 10uL of sample from the top of the tube into a hemocytometer and imaged using a microscope. If possible, cells will be counted. RBC present on samples will be compared to controls. Vibration on the tray will be performed for 1 hour as determined by vibration duration set by the customer requirements.

1.  Tray will be turned on to start vibration with the following testing conditions:

a.  All tubes filled at minimum fill volume

b.  All tubes filled at maximum fill volume

c.  Some tubes in tray at different fill volumes:

i.  1,3,5,8 tubes with varying fill volumes in range of acceptable fill volumes

d.  a-c repeated using different tube type (glass vs. plastic)

2.  Vibration will be used for 1 hour for each test run

3.  10uL of the sample will be aspirated from the top using a micropipette and will be imaged through a hemocytometer using a microscope

4.  Images will be obtained

5.  RBCs will be compared for samples in relation to the controls

6.  The relationship between tube location on the tray and RBC suspension will be analyzed by recording location of the tubes analyzed. This will allow for better analysis of the effect of the mass of the electrical components on RBC suspension.

Table 1. Experimental setup table for data collection.

Tube Type (Glass/Plastic) / Fill Volume (mL)
(3.5-4.5mL) / Number of Tubes in Tray (1-8) / Tube Location (1-8) / RBC Suspension Percentage Coverage
Glass / 3.5mL / No mixing / -
Plastic / 3.5mL / Inverting / -
Glass / 4.5mL / No mixing / -
Plastic / 4.5mL / Inverting / -
Glass / 3.5mL / No mixing / -
Plastic / 3.5mL / Inverting / -
Glass / 4.5mL / No mixing / -
Plastic / 4.5mL / Inverting / -
Glass / 3.5mL / 8 / 1
2
3
4
5
6
7
8
Plastic / 3.5mL / 8 / 1
2
3
4
5
6
7
8
Glass / 4.5mL / 8 / 1
2
3
4
5
6
7
8
Plastic / 4.5mL / 8 / 1
2
3
4
5
6
7
8
Glass / 1 / 1
2
3
4
5
6
7
8
Glass / 3 / 1
4
8
3
6
7
2
5
8
Glass / 5 / 1
2
4
6
8
2
4
6
7
8
1
3
5
6
8