Supplementary material

LINE1 methylation in granulocyte DNA and trihalomethane exposure on bladder cancer risk

Lucas A. Salas 1,2,3 / Cristina M. Villanueva 1,2,3,4 / Salman M. Tajuddin 5/ André F. S. Amaral 5 / Agustín F. Fernandez 6 / Lee E. Moore 7/ Alfredo Carrato 8,9/ Adonina Tardón 3,10/ Consol Serra 11,12/ Reina García-Closas13/ Xavier Basagaña 1,2,3 /Nathaniel Rothman 7 / Debra T. Silverman 7/ Kenneth P. Cantor 7/ Manolis Kogevinas 1,2,3,4,14/ Francisco X. Real 2,15/ Mario F. Fraga 6 /Núria Malats 5

1. Centre forResearch in EnvironmentalEpidemiology (CREAL), Barcelona, Spain / 2. UniversitatPompeu Fabra (UPF), Barcelona, Spain / 3. CIBER Epidemiología y Salud Pública (CIBERESP), Barcelona, Spain / 4. IMIM (Hospital del Mar Medical Research Institute) / 5. Genetic and Molecular Epidemiology Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain / 6. CancerEpigeneticsLaboratory, Instituto Universitario de Oncología del Principado de Asturias (IUOPA-HUCA), University of Oviedo, Oviedo, Spain / 7. National Cancer Institute, Bethesda, USA / 8.University Hospital of Elche, Elche, Spain / 9.Hospital Ramon y Cajal, Madrid / 10.University of Oviedo, Oviedo, Spain / 11.UniversitatPompeu Fabra (UPF). Centre of Research in Occupational Health, Spain / 12.ConsorciHospitalariParcTaulí, Sabadell, Spain / 13. Hospital Universitario de Canarias, La Laguna, Spain / 14. National School of Public Health, Athens, Greece / 15. Epithelial Carcinogenesis Group, Spanish National Cancer Research Centre (CNIO), Spain.

Address correspondence to C.M. Villanueva, CREAL- Centre for Research in Environmental Epidemiology, Barcelona Biomedical Research Park, Dr. Aiguader, 88, 08003 Barcelona, Spain. Telephone: (34) 93 2147300. Fax: (34) 932147302. E-mail: . N. Malats, Genetic and Molecular Epidemiology Group, Spanish National Cancer Research Centre (CNIO), MelchorFernándezAlmagro, 3, 28029, Madrid, Spain. Telephone: (34) 912246900. Fax (34) 912246980 E-mail:

Table of contents

Supplementary Material, Figure S1. Distribution of LINE1 %5-MeC, in average and by the four CpG in cases and controls.

Supplementary Material, Figure S2. Scatterplots of the first and second principal component from a principal component analysis of the 4 CpGs evaluated, according to different variables to test batch effects.

Supplementary Material, Figure S1. Distribution of LINE1 %5-MeC, in average and by the four CpG in cases and controls.

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Supplementary Material, Figure S2. Scatterplots of the first and second principal component from a principal component analysis of the 4 CpGs evaluated, according to different variables to test batch effects.

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