Supplementary Figure 1.

Supplementary Table 1. Identification of down-regulated proteins in SA7 cells.

SpotNo. / Accession
NCBI / Protein name / MW (kDa)
(Cal.)a) / pI
(Cal.) / Mowse score / No. match peptides
(% coverage)
PMF MS/MS / Foldb)
1 / P48538 / Galectin-1 (GAL1) / 14.8 / 5.52 / 95, 210 / 10/50 (61%) / 3 (28%) / 0.59
2 / P48538 / GAL1 / 14.8 / 5.52 / 129, 363 / 10/50 (61%) / 5 (42%) / 0.44
5 / AAB06983 / Phosphatidylethanolamine binding protein / 20.8 / 5.19 / --, 59 / -- / 2 (14%) / 0.1
6 / NP_031713 / Cofilin 1 (non-muscle) / 18.5 / 8.22 / 91, 90 / 10/50 (64%) / 2 (13%) / 0.37
9 / AAB19888 / Phosphoglycerate mutase 1 / 28.8 / 6.67 / 188, 257 / 16/50 (56%) / 4 (22%) / 0.27
11 / P48500 / Triosephosphate isomerase (TIM) / 26.7 / 7.07 / 87, 99 / 9/50 (41%) / 2 (5%) / 0.40
13 / NP_062615 / Stathmin 1 / 17.2 / 5.76 / 76, 99 / 8/50 (45%) / 1 (8%) / 0.36
14 / P48538 / GAL1 / 14.8 / 5.52 / 114, 198 / 10/50 (61%) / 3 (28%) / <0.1
18 / NP_080270 / EF hand domain containing 2 / 26.9 / 5.07 / 150, 261 / 15/50 (44%) / 5 (24%) / 0.68
20 / NP_032857 / Prohibitin / 29.8 / 5.57 / 135, 292 / 12/50 (58%) / 5 (25%) / 0.44
22 / AAA42263 / Trypomyosin / 28.7 / 4.69 / 116, 204 / 12/50 (27%) / 4 (16%) / 0.71
23 / P17244 / Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) / 35.7 / 8.49 / 103, 107 / 15/50 (41%) / 2 (8%) / 0.12
26 / ABM16832 / Beta-actin / 41.7 / 5.22 / 98, 220 / 13/50 (41%) / 3 (12%) / 0.85
28 / NP_446090 / Isocitrate dehydrogenase 3 (NAD+) alpha / 39.6 / 6.47 / 130, 313 / 17/50 (39%) / 5 (17%) / 0.81
29 / O08709 / Peroxiredoxin-6 (Prdx6) / 24.9 / 5.71 / 78, 150 / 7/50 (33%) / 2 (12%) / 0.2
30 / P14851 / Peptidyl-prolyl cis-trans isomerase A (PPIase A) / 17.9 / 8.44 / 120, 141 / 15/50 (68%) / 3 (25%) / 0.16
31 / AAB93667 / M2 pyruvate kinase (M2-PK) / 57.7 / 7.15 / 122, 184 / 19/50 (31%) / 3 (8%) / 0.20
32 / XP_889413 / High mobility group 1 protein / 24.8 / 5.75 / 117, 205 / 14/50 (41%) / 4 (19%) / 0.65

a) Cal.= Calculated molecular weight and pI obtained from Mascot database

(http://www.matrixscience.com/).

b) Fold = the proteins’ expression in SA7 cells compared with CHOA cells.

Supplemental MS/MS spectrum

Table 2 Spot 1

Table 2 Spot 3

Table 2 Spot 4

Table 2 Spot 5

Table 2 Spot 6

Table 2 Spot 7

Table 2 Spot 8

Table 2 Spot 9

Table 2 Spot 10

Table 2 Spot 12

Table 2 Spot 14

Table 2 Spot 15

Table 2 Spot 17

Table 2 Spot 18

Table 2 Spot 19

Table 2 Spot 20

Sup. Table 1 Spot 1

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Sup. Table 1 Spot 32