Table S1. Total Read Counts and Number of Otus Per Sample

Table S1. Total read counts and number of OTUs per sample

Sample / Archaea read count / Bacteria read count / Archaea OTUS / Bacteria OTUs
Ursu0.5m / 267 / 72 816 / 14 / 166
Ursu 2.5m / 340 / 71 048 / 10 / 285
Ursu 3.5m / 5 411 / 59 239 / 73 / 420
Ursu 9m / 20 822 / 50 779 / 116 / 459
Fara Fund0.5m / 83 184 / 6 401 / 74 / 127
Fara Fund 2m / 135 943 / 5 953 / 109 / 102
Fara Fund 3m / 86 284 / 8 086 / 70 / 57
Fara Fund 11m / 17 505 / 73 451 / 144 / 330

Table S2. The standardized Mantel test statistics (R) describing the correlations between archaeal and bacterial numbers, based on qPCR absolute quantification and environmental parameters

Ursu Lake / Fara Fund Lake
Bacteria / Archaea / Bacteria / Archaea
Parameter / Permutations / R / P / R / P / R / P / R / P
Salinity / 10,000 / 0.58 / 0.17 / 0.94 / 0.04* / 0.68 / 0.13 / 0.93 / 0.08
DO / 10,000 / -0.15 / 0.49 / 0.15 / 0.16 / -0.3 / 0.91 / -0.11 / 0.79
ORP / 10,000 / 0.65 / 0.11 / 0.94 / 0.32 / -0.05 / 0.33 / 0.2 / 0.25
T / 10,000 / -0.43 / 0.79 / -0.21 / 0.46 / -0.34 / 0.7 / 0.06 / 0.41
pH / 10,000 / 0.68 / 0.25 / 0.98 / 0.12 / 0.76 / 0.12 / 0.98 / 0.07
TCh / 10,000 / 0.62 / 0.24 / -0.13 / 0.91 / 0.21 / 0.33 / 0.54 / 0.25
TCa / 10,000 / 0.47 / 0.11 / -0.25 / 0.78 / 0.72 / 0.21 / 0.99 / 0.03*
NH4+-N / 10,000 / 0.43 / 0.32 / 0.83 / 0.21 / 0.58 / 0.12 / 0.91 / 0.08
NO3--N / 10,000 / -0.50 / 0.92 / -0.39 / 0.62 / 0.27 / 0.33 / -0.28 / 0.91
NO2--N / 10,000 / 0.84 / 0.03* / 0.43 / 0.15 / ND / ND / ND / ND
ON / 10,000 / 0.48 / 0.12 / 0.711 / 0.08 / 0.95 / 0.03* / 0.88 / 0.15
TN / 10,000 / 0.35 / 0.33 / 0.74 / 0.16 / 0.73 / 0.11 / 0.99 / 0.07
TOC / 10,000 / -0.42 / 1 / -0.14 / 0.88 / 0.33 / 0.34 / 0.32 / 0.32
SO42- / 10,000 / 0.27 / 0.33 / 0.67 / 0.04* / 0.34 / 0.28 / 0.62 / 0.25
HS- / 10,000 / ND / ND / ND / ND / ND / ND / ND / ND
PO43- / 10,000 / -0.6 / 1 / -0.33 / 0.7 / ND / ND / ND / ND

* Statistically significant correlation (P<0.05)

Abbreviations: DO-dissolved oxygen, ORP-oxidoreduction potential, T-temperature, TCh-total chlorophyll a, TCa-total carotenoids, NNH4+-ammonium nitrogen, ON-organic nitrogen, TN-total nitrogen, TOC-total organic carbon, ND-not determined, P-p value.

Table S3. Good’s coverage estimator for OTUs defined at ≥97% sequence similarity

Good’s coverage
Archaeaa / Bacteriab / Prokaryotesc
Ursu0.5m / N/A / 0.995 / 0.999
Ursu2.5m / N/A / 0.991 / 0.999
Ursu3.5m / 0.999 / 0.985 / 0.998
Ursu9m / 0.996 / 0.983 / 0.997
Fara Fund0.5m / 0.997 / 0.993 / 0.998
Fara Fund 2m / 0.996 / 0.994 / 0.998
Fara Fund 3m / 0.998 / 0.999 / 0.999
Fara Fund 11m / 0.995 / 0.988 / 0.998

aThe archaeal counts were rarefied at 5411 sequences

bThe bacterial counts were rarefied at 5953 sequences

cThe prokaryotic counts were rarefied at 64 650 sequences

N/A: not applicable

Table S4. Pearson correlation statistics (r) between prokaryotic alpha diversity (measured as Faith’s PD) and environmental parameters

Archaeal alpha diversity (Faith’s PD) / Bacterial alpha diversity (Faith’s PD)
Parameter / r / p / r / p
Salinity / 0.770 / 0.073 / 0.382 / 0.350
DO / -0.454 / 0.367 / -0.793 / 0.019*
ORP / -0.551 / 0.258 / -0.774 / 0.024*
T / -0.608 / 0.200 / -0.443 / 0.273
pH / -0.875 / 0.023* / -0.516 / 0.192
TCh / 0.431 / 0.393 / 0.173 / 0.682
TCa / 0.361 / 0.482 / 0.338 / 0.413
NH4+-N / 0.921 / 0.009* / 0.684 / 0.061
NO3--N / -0.253 / 0.628 / -0.333 / 0.420
NO2--N / ND / ND / ND / ND
ON / -0.829 / 0.042* / -0.522 / 0.184
TN / 0.862 / 0.027* / 0.713 / 0.047*
TOC / 0.001 / 0.997 / 0.453 / 0.259
CO3- / 0.783 / 0.066 / 0.678 / 0.065
HCO3- / 0.849 / 0.033* / 0.741 / 0.036*
Cl- / 0.844 / 0.034* / 0.415 / 0.306
SO42- / -0.156 / 0.767 / -0.063 / 0.884
HS- / ND / ND / ND / ND
PO43- / ND / ND / ND / ND
K+ / -0.682 / 0.135 / -0.303 / 0.467
Fe / 0.647 / 0.165 / 0.298 / 0.474
Ca2+ / 0.696 / 0.124 / 0.322 / 0.436
Mg2+ / -0.133 / 0.803 / -0.152 / 0.721
Na+ / 0.848 / 0.032* / 0.424 / 0.295
Depth / 0.884 / 0.019* / 0.657 / 0.077

* Statistically significant correlation (P<0.05)

Abbreviations: DO-dissolved oxygen, ORP-oxidoreduction potential, T-temperature, TCh-total chlorophyll a, TCa-total carotenoids, NNH4+-ammonium nitrogen, ON-organic nitrogen, TN-total nitrogen, TOC-total organic carbon, ND-not determined, P-p value.

Table S5. Taxonomic composition of Ursu and Fara Fund lakes

Phylum-level taxonomy*
Phylum / Abundance / Depth
(m) / Lake / Phylum / Abundance / Depth
(m) / Lake
Verrucomicrobia / 1% / 0.5 / Ursu / Proteobacteria / 17% / 9 / Ursu
Tenericutes / 5% / 0.5 / Ursu / OP1 / 3% / 9 / Ursu
Proteobacteria / 16% / 0.5 / Ursu / OD1 / 4% / 9 / Ursu
Bacteroidetes / 26% / 0.5 / Ursu / Lentisphaerae / 1% / 9 / Ursu
Actinobacteria / 50% / 0.5 / Ursu / Firmicutes / 14% / 9 / Ursu
Verrucomicrobia / 1% / 2.5 / Ursu / Bacteroidetes / 11% / 9 / Ursu
Tenericutes / 2% / 2.5 / Ursu / Actinobacteria / 5% / 9 / Ursu
Proteobacteria / 19% / 2.5 / Ursu / Parvarchaeota / 2% / 9 / Ursu
Cyanobacteria / 10% / 2.5 / Ursu / Euryarchaeota / 26% / 9 / Ursu
Chlorobi / 7% / 2.5 / Ursu / Proteobacteria / 2% / 0.5 / Fara Fund
Bacteroidetes / 19% / 2.5 / Ursu / Bacteroidetes / 3% / 0.5 / Fara Fund
Actinobacteria / 40% / 2.5 / Ursu / Euryarchaeota / 95% / 0.5 / Fara Fund
WWE1 / 3% / 3.5 / Ursu / Bacteroidetes / 2% / 2 / Fara Fund
Proteobacteria / 19% / 3.5 / Ursu / Euryarchaeota / 96% / 2 / Fara Fund
OP1 / 2% / 3.5 / Ursu / Bacteroidetes / 6% / 3 / Fara Fund
OD1 / 2% / 3.5 / Ursu / Parvarchaeota / 2% / 3 / Fara Fund
Lentisphaerae / 3% / 3.5 / Ursu / Euryarchaeota / 89% / 3 / Fara Fund
Firmicutes / 22% / 3.5 / Ursu / Proteobacteria / 42% / 11 / Fara Fund
Cyanobacteria / 1% / 3.5 / Ursu / OP1 / 2% / 11 / Fara Fund
Chlorobi / 7% / 3.5 / Ursu / OD1 / 3% / 11 / Fara Fund
Bacteroidetes / 5% / 3.5 / Ursu / Firmicutes / 14% / 11 / Fara Fund
Actinobacteria / 4% / 3.5 / Ursu / Bacteroidetes / 15% / 11 / Fara Fund
Euryarchaeota / 8% / 3.5 / Ursu / Euryarchaeota / 18% / 11 / Fara Fund
Order-level taxonomy*
Order / Abundance / Depth
(m) / Lake / Order / Abundance / Depth
(m) / Lake
Verrucomicrobiales / 1% / 0.5 / Ursu / Desulfobacterales / 1% / 3.5 / Ursu
Marinicellales / 1% / 0.5 / Ursu / Halanaerobiales / 21% / 3.5 / Ursu
Enterobacteriales / 1% / 0.5 / Ursu / Clostridiales / 1% / 3.5 / Ursu
Chromatiales / 2% / 0.5 / Ursu / Synechococcales / 1% / 3.5 / Ursu
Alteromonadales / 3% / 0.5 / Ursu / Chlorobiales / 7% / 3.5 / Ursu
Rhodobacterales / 3% / 0.5 / Ursu / Actinomycetales / 4% / 3.5 / Ursu
Saprospirales / 4% / 0.5 / Ursu / Methanobacteriales / 6% / 3.5 / Ursu
Rhodothermales / 4% / 0.5 / Ursu / Halobacteriales / 1% / 3.5 / Ursu
Sphingobacteriales / 5% / 0.5 / Ursu / Oceanospirillales / 1% / 9 / Ursu
Flavobacteriales / 6% / 0.5 / Ursu / Desulfovibrionales / 12% / 9 / Ursu
Cytophagales / 6% / 0.5 / Ursu / Desulfobacterales / 1% / 9 / Ursu
Nitriliruptorales / 15% / 0.5 / Ursu / Halanaerobiales / 14% / 9 / Ursu
Actinomycetales / 35% / 0.5 / Ursu / Actinomycetales / 4% / 9 / Ursu
Chromatiales / 3% / 2.5 / Ursu / Methanobacteriales / 12% / 9 / Ursu
Alteromonadales / 2% / 2.5 / Ursu / Halobacteriales / 7% / 9 / Ursu
Rhodospirillales / 2% / 2.5 / Ursu / Rhodothermales / 2% / 0.5 / Fara Fund
Rhodobacterales / 5% / 2.5 / Ursu / Halobacteriales / 95% / 0.5 / Fara Fund
Synechococcales / 10% / 2.5 / Ursu / Rhodothermales / 2% / 2 / Fara Fund
Chlorobiales / 6% / 2.5 / Ursu / Halobacteriales / 96% / 2 / Fara Fund
Saprospirales / 6% / 2.5 / Ursu / Rhodothermales / 6% / 3 / Fara Fund
Rhodothermales / 9% / 2.5 / Ursu / Halobacteriales / 88% / 3 / Fara Fund
Flavobacteriales / 3% / 2.5 / Ursu / Desulfovibrionales / 39% / 11 / Fara Fund
Nitriliruptorales / 15% / 2.5 / Ursu / Desulfobacterales / 1% / 11 / Fara Fund
Actinomycetales / 25% / 2.5 / Ursu / Halanaerobiales / 14% / 11 / Fara Fund
Desulfovibrionales / 13% / 3.5 / Ursu / Halobacteriales / 16% / 11 / Fara Fund
Family-level taxonomy*
Family / Abundance / Depth
(m) / Lake / Family / Abundance / Depth
(m) / Lake
Halobacteriaceae / 1% / 3.5 / Ursu / Nitriliruptoraceae / 15% / 2.5 / Ursu
Halobacteriaceae / 6% / 9 / Ursu / Microbacteriaceae / 12% / 2.5 / Ursu
Verrucomicrobiaceae / 1% / 0.5 / Ursu / Desulfohalobiaceae / 5% / 3.5 / Ursu
Marinicellaceae / 1% / 0.5 / Ursu / Desulfobacteraceae / 1% / 3.5 / Ursu
Enterobacteriaceae / 1% / 0.5 / Ursu / Rhodobacteraceae / 1% / 3.5 / Ursu
OM60 / 2% / 0.5 / Ursu / Halanaerobiaceae / 21% / 3.5 / Ursu
Alcaligenaceae / 1% / 0.5 / Ursu / Synechococcaceae / 1% / 3.5 / Ursu
Rhodobacteraceae / 3% / 0.5 / Ursu / Chlorobiaceae / 7% / 3.5 / Ursu
Saprospiraceae / 4% / 0.5 / Ursu / Desulfohalobiaceae / 9% / 9 / Ursu
Balneolaceae / 4% / 0.5 / Ursu / Desulfobacteraceae / 1% / 9 / Ursu
NS11-12 / 5% / 0.5 / Ursu / Halobacteroidaceae / 1% / 9 / Ursu
Flavobacteriaceae / 4% / 0.5 / Ursu / Halanaerobiaceae / 12% / 9 / Ursu
Flammeovirgaceae / 1% / 0.5 / Ursu / Halobacteriaceae / 95% / 0.5 / Fara Fund
Cyclobacteriaceae / 5% / 0.5 / Ursu / Halobacteriaceae / 96% / 2 / Fara Fund
Nitriliruptoraceae / 15% / 0.5 / Ursu / Halobacteriaceae / 40% / 3 / Fara Fund
Microbacteriaceae / 19% / 0.5 / Ursu / Halobacteriaceae / 17% / 11 / Fara Fund
Marinicellaceae / 1% / 2.5 / Ursu / Balneolaceae / 2% / 0.5 / Fara Fund
Rhodospirillaceae / 2% / 2.5 / Ursu / Balneolaceae / 2% / 2 / Fara Fund
Rhodobacteraceae / 5% / 2.5 / Ursu / Rhodothermaceae / 5% / 3 / Fara Fund
Synechococcaceae / 10% / 2.5 / Ursu / Desulfohalobiaceae / 39% / 11 / Fara Fund
Chlorobiaceae / 6% / 2.5 / Ursu / Desulfobacteracea / 1% / 11 / Fara Fund
Saprospiraceae / 6% / 2.5 / Ursu / Halobacteroidaceae / 6% / 11 / Fara Fund
Balneolaceae / 9% / 2.5 / Ursu / Halanaerobiaceae / 8% / 11 / Fara Fund
Chryomorphaceae / 1% / 2.5 / Ursu
Genus-level taxonomy*
Genus / Abundance / Depth
(m) / Lake / Genus / Abundance / Depth
(m) / Lake
Balneola / 1% / 0.5 / Ursu / Desulfovermiculus / 6% / 9 / Ursu
Psychroflexus / 3% / 0.5 / Ursu / Acetohalobium / 1% / 9 / Ursu
Algoriphagus / 3% / 0.5 / Ursu / Halanaerobium / 8% / 9 / Ursu
Synechococcus / 10% / 2.5 / Ursu / Salinibacter / 5% / 3 / Fara Fund
Prosthecochloris / 6% / 2.5 / Ursu / Halorubrum / 4% / 3 / Fara Fund
Balneola / 4% / 2.5 / Ursu / Halorhabdus / 2% / 3 / Fara Fund
Owenweeksia / 1% / 2.5 / Ursu / Desulfovermiculus / 3% / 11 / Fara Fund
Desulfovermiculus / 2% / 3.5 / Ursu / Halanaerobacter / 6% / 11 / Fara Fund
Halanaerobium / 17% / 3.5 / Ursu / Halanaerobium / 6% / 11 / Fara Fund
Synechococcus / 1% / 3.5 / Ursu / Halorubrum / 1% / 11 / Fara Fund
Prosthecochloris / 7% / 3.5 / Ursu / Halorhabdus / 10% / 11 / Fara Fund

*Table contains taxa with an abundance ≥1%

Table S6. Identified taxa* known to be involved in biogeochemical processes

Cycle, Taxa and Location / Cycle, Taxa and Location
PHOTOSYNTHESIS
Oxygenic Photosynthesis
Cyanobacteria: Ursu Lake
Anoxygenic Photosynthesis
Chlorobi:Ursu Lake
Proteobacteria-Ectothiorhodospiraceae: Fara Fund Lake
Proteobacteria-Rhodobacterales: Ursu and Fara Fund lakes
AEROBIC C-DEGRADATION
Euryarchaeota-209ZB06: Ursu and Fara Fund lakes
Euryarchaeota-Haloarcula: Fara Fund Lake
Euryarchaeota-Halobacterium: Fara Fund Lake
Euryarchaeota-Halobaculum: Fara Fund Lake
Euryarchaeota-Halobellus: Fara Fund Lake
Euryarchaeota-Halolamina: Fara Fund Lake
Euryarchaeota-Halomicrobium: Fara Fund Lake
Euryarchaeota-Halonotius: Fara Fund Lake
Euryarchaeota-Haloplanus: Fara Fund Lake
Euryarchaeota-Halorhabdus:Ursu and Fara Fund lakes
Euryarchaeota-Halorubrum: Fara Fund Lake
Euryarchaeota-Natronomonas: Fara Fund Lake
Nanohaloarchaeota-MSP41:Ursu and Fara Fund lakes
Actinobacteria-Microbacteriaceae: Ursu Lake
Actinobacteria-Nitriliruptoraceae: Ursu Lake
Bacteroidetes-Balneola: Ursu and Fara Fund lakes
Bacteroidetes-KSA1: Ursu and Fara Fund lakes
Bacteroidetes-Saprospiraceae: Ursu and Fara Fund lakes
Bacteroidetes-Flavobacteriales: Ursu and Fara Fund lakes
Bacteroidetes-Cryomorphaceae: Ursu and Fara Fund lakes
Bacteroidetes-Flavobacteriaceae: Ursu and Fara Fund lakes
Proteobacteria-Alteromonadales: Ursu Lake
Proteobacteria-Alteromonadaceae: Ursu and Fara Fund lakes
Proteobacteria-HTCC2188: Ursu Lake
Proteobacteria-OM60: Ursu Lake
Verrucomicrobia-Puniceicoccaceae: Ursu Lake
Verrucomicrobia-WCHB1-41: Ursu Lake
Verrucomicrobia-Verrucomicrobiaceae: Ursu Lake
Proteobacteria-Enterobacteriaceae: Ursu Lake
Bacteroidetes-Flammeovirgaceae: Ursu and Fara Fundlakes
Bacteroidetes-Roseivirga: Ursu Lake
Proteobacteria-Rhodospirillaceae: Ursu and Fara Fund lakes
Proteobacteria-Vibrio: Ursu Lake
Proteobacteria-Oceanospirillales: Ursu and Fara Fund lakes
Proteobacteria-Halomonadaceae: Ursu and Fara Fund lakes
Proteobacteria-Oceanospirillaceae: Ursu Lake
Proteobacteria-Saccharospirillaceae: Ursu Lake
ANAEROBIC C-DEGRADATION
Euryarchaeota-Halorhabdus:Ursu and Fara Fund lakes
Firmicutes-Halanaerobiaceae: Ursu and Fara Fund lakes
Firmicutes-Halanaerobium:Ursu and Fara Fund lakes
Firmicutes-Acetohalobium: Ursu and Fara Fund lakes
Firmicutes-Halanaerobacter: Ursu and Fara Fund lakes
Firmicutes-Anaerovirgula: Ursu Lake
Firmicutes-Desulfitobacter: Ursu and Fara Fund lakes
Firmicutes-NP25: Ursu and Fara Fund lakes
Firmicutes-Thermoanaerobacterales Family III:Fara Fund Lake
Euryarchaeota-Methanobacteriales:Ursu and Fara Fund lakes
Euryarchaeota-MSBL1:Ursu and Fara Fund lakes
Euryarchaeota-Methanosarcinaceae:Ursu and Fara Fund lakes
OD1:Ursu and Fara Fund lakes
OP1:Ursu and Fara Fund lakes / SULPHUR CYCLE
S-oxidizers
Proteobacteria-Thiomicrospira: Ursu Lake
Proteobacteria-Chromatiales: Ursu and Fara Fund lakes
Proteobacteria-Ectothiorhodospiraceae: Fara Fund Lake
Chlorobi-Prosthecochloris: Ursu Lake
Chlorobi-OPB56: Ursu Lake
Proteobacteria-Thiohalorhabdus: Fara Fund Lake
S-reducers
Proteobacteria-Desulfobacteraceae: Ursu and Fara Fund lakes
Proteobacteria-Desulfobulbaceae: Ursu Lake
Proteobacteria-Desulfovibrionales: Ursu Lake
Proteobacteria-Desulfuromonas: Ursu Lake
Firmicutes-Desulfitobacter: Ursu and Fara Fund lakes
NITROGEN CYCLE
N-assimilation
Proteobacteria-Rhodospirillaceae- Ursu and Fara Fund lakes
Denitrification
Proteobacteria-Halomonas: Ursu and Fara Fund lakes
Proteobacteria-Rhodospirillaceae: Ursu Lake
Proteobacteria-Sulfurimonas: Ursu Lake
Proteobacteria-Thiohalorhabdus: Fara Fund Lake
Proteobacteria-Marinobacter: Fara Fund Lake
Proteobacteria-Bradyrhizobium: Fara Fund Lake
Proteobacteria-Phaeobacter: Ursu Lake
Nitrification
Planctomycetes: Ursu and Fara Fund lakes
METHANE CYCLE
Methanogenesis
Euryarchaeota-Methanobacteriales: Ursu and Fara Fund lakes
Euryarchaeota-MSBL1: Ursu and Fara Fund lakes
Euryarchaeota-Methanosarcinaceae: Ursu and Fara Fund lakes
Methanotrophy
Proteobacteria-Methylococcales: Ursu Lake
Fe/Mn CYCLE
Deferribacteres:Ursu and Fara Fund lakes
Proteobacteria-Desulfuromonas: Ursu Lake

*The identified taxa have an abundance of ≥0.1%

Table S7. Nearest BLAST hits to the methanogenic prokaryotes in Ursu and Fara Fund lakes

OTU* / Classification** / Number of sequences / Nearest match (with% identity) / Source location of the match / GenBank ID
12 / Methanobacteriales / 11,388 / (98%) Clone HS-EN13A / Mediterranean solar saltern-sediments / FJ536516.1
166 / MSBL1 / 247 / (96%) Clone HS-LN11A / Mediterranean solar saltern-sediments / FJ536505.1
164 / MSBL1 / 234 / (96%) Clone HS-LN1A / Mediterranean solar saltern-sediments / FJ536518.1
246 / MSBL1 / 126 / (95%) Clone MEDEE_16SA_C4_BB / DHAB Lake Medee-water / JX446286.1
353 / MSBL1 / 107 / (97%) Clone MEDEE_16SA_C4_BB / DHAB Lake Medee-water / JX446286.1
348 / MSBL1 / 103 / (93%) Clone Discovery_a / Red Sea hydrothermal brine-water / HQ530525.1
899 / MSBL1 / 102 / (98%) Clone MEDEE_16SA_C4_BB / DHAB Lake Medee-water / JX446286.1
370 / MSBL1 / 100 / (100%) Clone HS-LN10A / Mediterranean solar saltern-sediments / FJ536508.1
649 / MSBL1 / 66 / (100%) Clone MEDEE_ENRGB_G2_BA / DHAB Lake Medee-water / KC424646.1
523 / MSBL1 / 60 / (99%) Clone MEDEE_16SA_B12_BB / DHAB Lake Medee-water / JX446213.1
365 / MSBL1 / 53 / (99%) Clone MEDEE_ENRTMA_F5_B / DHAB Lake Medee-water / KF156263.1
3622 / MSBL1 / 33 / (98%) Clone MEDEE_16SA_B12_BB / DHAB Lake Medee-water / JX446213.1
1058 / DL1A-100 / 25 / (98%) Clone BA1 / Aran-Bidgol Salt Lake-water / HQ425147.1
898 / MSBL1 / 21 / (95%) Clone WN-USA-157 / Hypersaline lakes of the Wadi An Natrun-water / DQ432573.1
1408 / MSBL1 / 19 / (96%) Clone MEDEE_16SA_D6_BB / DHAB Lake Medee-water / JX446283.1
1137 / MSBL1 / 5 / (95%) Clone T3A_A7 / DHAB Lake Thetis-water / HQ658688.1

* The OTUs are organized by decreasing abundance. **The classification represents the finest-possible level of taxonomic resolution. Abbreviations: MSBL1-Mediterranean Sea Brine Lake Group 1; DHAB-deep sea-hypersaline anoxic basins.