Supplementary materials
Optimization and evaluation of sugars and chemicals production from green macro-alga Enteromorpha intestinalis
Dong-Hyun Kim1, Sang-Bum Lee1, Sung-Koo Kim1, Don-Hee Park2,3, Gwi-Taek Jeong1*
1Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea
2Department of Biotechnology and Bioengineering, 3Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju 61186, Republic of Korea
Table S1. ANOVA results for the response surface quadratic model for glucose production
Source / Sum of squares / Degree of freedom / Mean square / F value / P value(Prob > Fa,b)
Model / 37.04 / 9 / 4.12 / 12.62017 / 0.0002
A:Temp. / 18.18 / 1 / 18.18 / 55.74321 / < 0.0001
B:Catalyst / 4.00 / 1 / 4.00 / 12.27502 / 0.0057
C:Time / 6.71 / 1 / 6.71 / 20.58294 / 0.0011
AB / 0.15 / 1 / 0.15 / 0.467174 / 0.5098
AC / 0.10 / 1 / 0.10 / 0.320346 / 0.5839
BC / 0.31 / 1 / 0.31 / 0.962336 / 0.3497
A2 / 5.45 / 1 / 5.45 / 16.72054 / 0.0022
B2 / 0.00 / 1 / 0.00 / 0.015328 / 0.9039
C2 / 2.73 / 1 / 2.73 / 8.371369 / 0.0160
Residual / 3.26 / 10 / 0.33
Lack of Fit / 3.11 / 5 / 0.62 / 19.99357 / 0.0026
Pure Error / 0.16 / 5 / 0.03
Cor Total / 40.30 / 19
a Prob > F = level of significance.
b Values of "Prob > F" less than 0.05 indicate model terms are significant.
Table S2. ANOVA results for the response surface quadratic model for XMG production
Source / Sum of squares / Degree of freedom / Mean square / F value / P value(Prob > Fa,b)
Model / 103.74 / 9 / 11.53 / 18.64119 / < 0.0001
A:Temp. / 46.18 / 1 / 46.18 / 74.68943 / < 0.0001
B:Catalyst / 15.61 / 1 / 15.61 / 25.24457 / 0.0005
C:Time / 11.44 / 1 / 11.44 / 18.4946 / 0.0016
AB / 4.67 / 1 / 4.67 / 7.557386 / 0.0205
AC / 3.28 / 1 / 3.28 / 5.297704 / 0.0441
BC / 1.18 / 1 / 1.18 / 1.900626 / 0.1981
A2 / 18.38 / 1 / 18.38 / 29.72478 / 0.0003
B2 / 2.68 / 1 / 2.68 / 4.338212 / 0.0639
C2 / 3.09 / 1 / 3.09 / 4.997358 / 0.0494
Residual / 6.18 / 10 / 0.62
Lack of Fit / 5.52 / 5 / 1.10 / 8.355947 / 0.0180
Pure Error / 0.66 / 5 / 0.13
Cor Total / 109.92 / 19
a Prob > F = level of significance.
b Values of "Prob > F" less than 0.05 indicate model terms are significant.
Table S3. ANOVA results for the response surface quadratic model for TRS production
Source / Sum of squares / Degree of freedom / Mean square / F value / P value(Prob > Fa,b)
Model / 338.66 / 9 / 37.63 / 32.3814 / < 0.0001
A:Temp. / 167.40 / 1 / 167.40 / 144.0597 / < 0.0001
B:Catalyst / 69.47 / 1 / 69.47 / 59.78293 / < 0.0001
C:Time / 38.59 / 1 / 38.59 / 33.20444 / 0.0002
AB / 8.09 / 1 / 8.09 / 6.960808 / 0.0248
AC / 2.59 / 1 / 2.59 / 2.224951 / 0.1667
BC / 0.33 / 1 / 0.33 / 0.283565 / 0.6060
A2 / 36.98 / 1 / 36.98 / 31.82682 / 0.0002
B2 / 11.78 / 1 / 11.78 / 10.13496 / 0.0098
C2 / 12.29 / 1 / 12.29 / 10.5785 / 0.0087
Residual / 11.62 / 10 / 1.16
Lack of Fit / 9.10 / 5 / 1.82 / 3.602151 / 0.0929
Pure Error / 2.53 / 5 / 0.51
Cor Total / 350.28 / 19
a Prob > F = level of significance.
b Values of "Prob > F" less than 0.05 indicate model terms are significant.
Table S4. ANOVA results for the response surface quadratic model for 5-HMF production
Source / Sum of squares / Degree of freedom / Mean square / F value / P value(Prob > Fa,b)
Model / 1.85 / 9 / 0.21 / 4.541537 / 0.0135
A:Temp. / 0.00 / 1 / 0.00 / 0.057032 / 0.8161
B:Catalyst / 0.28 / 1 / 0.28 / 6.215371 / 0.0318
C:Time / 0.03 / 1 / 0.03 / 0.673289 / 0.4310
AB / 0.61 / 1 / 0.61 / 13.4235 / 0.0044
AC / 0.00 / 1 / 0.00 / 0.038593 / 0.8482
BC / 0.01 / 1 / 0.01 / 0.230789 / 0.6413
A2 / 0.44 / 1 / 0.44 / 9.701625 / 0.0110
B2 / 0.55 / 1 / 0.55 / 12.07077 / 0.0060
C2 / 0.07 / 1 / 0.07 / 1.524519 / 0.2452
Residual / 0.45 / 10 / 0.05
Lack of Fit / 0.26 / 5 / 0.05 / 1.316504 / 0.3851
Pure Error / 0.20 / 5 / 0.04
Cor Total / 2.30 / 19
a Prob > F = level of significance.
b Values of "Prob > F" less than 0.05 indicate model terms are significant.
Table S5. ANOVA results for the response surface quadratic model for FF production
(Prob > Fa,b)
Model / 1.91 / 9 / 0.21 / 110.3763 / < 0.0001
A:Temp. / 0.09 / 1 / 0.09 / 47.89948 / < 0.0001
B:Catalyst / 0.02 / 1 / 0.02 / 8.383813 / 0.0160
C:Time / 0.10 / 1 / 0.10 / 51.42582 / < 0.0001
AB / 0.60 / 1 / 0.60 / 314.6286 / < 0.0001
AC / 0.26 / 1 / 0.26 / 135.2954 / < 0.0001
BC / 0.07 / 1 / 0.07 / 36.61846 / 0.0001
A2 / 0.48 / 1 / 0.48 / 250.1172 / < 0.0001
B2 / 0.36 / 1 / 0.36 / 188.0329 / < 0.0001
C2 / 0.01 / 1 / 0.01 / 5.413501 / 0.0423
Residual / 0.02 / 10 / 0.00
Lack of Fit / 0.01 / 5 / 0.00 / 2.788669 / 0.1424
Pure Error / 0.01 / 5 / 0.00
Cor Total / 1.93 / 19
a Prob > F = level of significance.
b Values of "Prob > F" less than 0.05 indicate model terms are significant.
(A) (B)
(C)
Figure S1. Response surface plot representing the effect of reaction factors, and their reciprocal interaction on the formation of glucose from E. intestinalis at a constant reaction condition. (A) 35 min; (B) 2 %; (C) 175 °C.
(A) (B)
(C)
Figure S2. Response surface plot representing the effect of reaction factors, and their reciprocal interaction on the formation of XMG from E. intestinalis at a constant reaction condition. (A) 35 min; (B) 2 %; (C) 175 °C.
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