Supplemental Table S2 Sequence information of polymorphic InDel and SSR markers used in this study

Loci / Scaffold / Primer start / Primerend / Product size (bp) / Forward primer sequence (5' to 3') / Reverse primer sequence (5' to 3') / Ho / He / H / PIC
S306_D1 / Le_N7_S306 / 16216 / 16398 / 183 / GAGAGGGCGATGAATGAGAG / ACTTCGAATCTTTCGTGCGT / 0.375 / 0.596 / 0.593 / 0.555
S292_D1 / Le_N7_S292 / 13816 / 14041 / 226 / CTGTAGCACTTACGCACGGA / ATTGTCCTGGATTCGCACTC / 0.419 / 0.566 / 0.563 / 0.523
S240_D1 / Le_N7_S240 / 157735 / 158003 / 269 / GCTGTTAGGGAGGGAAGAGC / ACTGCCTGCTTGGTTGAACT / 0.322 / 0.601 / 0.597 / 0.512
S504_D1 / Le_N7_S504 / 5820 / 6104 / 285 / GCAATCGAGACTGATGCAAA / TCTCTAGGGACGCAGTCGAT / 0.471 / 0.778 / 0.773 / 0.739
S253_D1 / Le_N7_S253 / 53453 / 53640 / 188 / TGTCTCCCACTCTTTTTCGG / AGTTGCGAGAACAGATGGCT / 0.162 / 0.176 / 0.174 / 0.165
S462_D1 / Le_N7_S462 / 159458 / 159623 / 166 / GGTGAAACGAACCTGGAAAA / CAACGATCTTTGGTGGGAGT / 0.134 / 0.126 / 0.125 / 0.117
S253_D2 / Le_N7_S253 / 75211 / 75400 / 190 / ACGACATCACCAATGCTTCA / TCAGGCGACACCTGTCTATG / 0.103 / 0.099 / 0.098 / 0.093
S480_D1 / Le_N7_S480 / 7998 / 8232 / 235 / AGGAGAAGGAGATTGGGGAA / TGTTAGAGAAATGGGCAGGG / 0.159 / 0.147 / 0.146 / 0.136
S243_D2 / Le_N7_S243 / 73293 / 73586 / 294 / TAACGGCTTCTGTCAGGCTT / GAATTCGCTCGAGTCCTTTG / 0.141 / 0.513 / 0.510 / 0.430
S384_D1 / Le_N7_S384 / 35988 / 36255 / 268 / CACATGTTCCTGTGGATTCG / GGTGAAGGGACGAGTGGTAA / 0.233 / 0.401 / 0.399 / 0.364
S458_D1 / Le_N7_S458 / 2164 / 2407 / 244 / ACAAAGGAGACTTGCGGAAA / TTGAGCGACGTACCTGACTG / 0.374 / 0.860 / 0.854 / 0.838
S53_D3 / Le_N7_S53 / 672047 / 672227 / 181 / CCAGTGGTATCGTCCCAGTT / ACCTGTTGGACAGCCTCATC / 0.659 / 0.811 / 0.806 / 0.780
S395_D1 / Le_N7_S395 / 97883 / 98093 / 211 / GTGGAAGCCAATCTTCGTGT / TTGAGCTTTCCCAGCAGTCT / 0.375 / 0.416 / 0.413 / 0.377
S313_D1 / Le_N7_S313 / 78284 / 78525 / 242 / GCGTCATCGGTAATTTCGTT / CATTCGACCATCTGGTCCTT / 0.068 / 0.066 / 0.066 / 0.064
S476_D2 / Le_N7_S476 / 88596 / 88771 / 176 / TGGTGAATCAGAACGGTCAA / ATGCGCGAAATTGTAAAAGG / 0.552 / 0.443 / 0.440 / 0.366
S438_D2 / Le_N7_S438 / 21234 / 21413 / 180 / GCCGGTCATTGAACACTATG / TTAGGCGGTAGAGCCAGAAA / 0.318 / 0.398 / 0.396 / 0.357
S649_D1 / Le_N7_S649 / 40105 / 40304 / 200 / TGTTTATACCCCCAGCGAAG / TGTGAGCTTGCGATTTGAAC / 0.125 / 0.141 / 0.140 / 0.133
S72_D2 / Le_N7_S72 / 15908 / 16137 / 230 / GGGGACTTACCAGTCAGCAA / TGACGGTGCCTACGATATGA / 0.250 / 0.305 / 0.303 / 0.266
S60_D1 / Le_N7_S60 / 43758 / 43976 / 219 / ACCTCATCATCCCCTTTTCC / TGCGAAGGGAATCCATAAAG / 0.261 / 0.449 / 0.447 / 0.376
S126_E1 / Le_N7_S126 / 19794 / 20012 / 219 / TCCCTGCGTAAACGATAACC / AGCTTGGACTGCTGACCATT / 0.159 / 0.557 / 0.554 / 0.488
S131_E1 / Le_N7_S131 / 58451 / 58721 / 271 / GTTGCTTGAAGCTTTCGCTC / GTGTGAAATGCCTGTGATGG / 0.259 / 0.541 / 0.538 / 0.490
S138_E1 / Le_N7_S138 / 2337 / 2609 / 273 / AGTGAACGGTCTATCCGGTG / CCTGTTTCCGGTGTTTCCTA / 0.151 / 0.141 / 0.140 / 0.130
S143_E1 / Le_N7_S143 / 13427 / 13575 / 149 / GAAACTTGAAGCCGTTCGAC / GGATAAACAAGCGCGAGAAG / 0.224 / 0.432 / 0.429 / 0.337
S180_E1 / Le_N7_S180 / 23900 / 24198 / 299 / TTCTTTTCAACGTTCGCCTC / CAACACGCCTCGAGTTTACA / 0.581 / 0.532 / 0.529 / 0.472
S435_E1 / Le_N7_S435 / 59361 / 59537 / 177 / ACAGCCTGAACTGCTACCGT / CCCTTTCCACAGTGTCTGGT / 0.119 / 0.263 / 0.262 / 0.227
S313_E1 / Le_N7_S313 / 10852 / 11079 / 228 / CAACAGCGTTTTGTGGAATG / CCCCAAATTCCTCAGCTACA / 0.068 / 0.066 / 0.066 / 0.064
S32_E1 / Le_N7_S32 / 5140 / 5388 / 249 / GTAATCATCGCGCCTTTTTC / AGCGAGCTATGACGACGATT / 0.325 / 0.296 / 0.294 / 0.260
S333_E1 / Le_N7_S333 / 65851 / 66068 / 218 / TGGTGCTCAGTTTGCCATTA / AACAACATCGACAGCAGCAG / 0.250 / 0.570 / 0.566 / 0.498
S384_E1 / Le_N7_S384 / 21792 / 21943 / 152 / AGCATTTCGTGCCCATATTC / GTCACCTTCGACGATATGCC / 0.667 / 0.706 / 0.702 / 0.656
S442_E1 / Le_N7_S442 / 81826 / 82103 / 278 / CAATTGAGTGTTCGCACCAT / CCAGCAGTTGTCACTTCGTC / 0.317 / 0.506 / 0.503 / 0.467
S217_E1 / Le_N7_S217 / 55246 / 55496 / 251 / AACCACGGTTCCTGAATGAC / TCTACACTTTCCCGACACCC / 0.273 / 0.341 / 0.339 / 0.281
S603_E1 / Le_N7_S603 / 77487 / 77731 / 245 / CAATCCATTCCCAGCTCTGT / GTTGGGAAAGAATGGCTGAA / 0.114 / 0.166 / 0.165 / 0.152
S66_E1 / Le_N7_S66 / 42378 / 42582 / 205 / TCTTCCCCCTCTTCGAATCT / AAGTCGAGGAAGCAAAGTCG / 0.388 / 0.802 / 0.797 / 0.766
S247_E1 / Le_N7_S247 / 82635 / 82786 / 152 / CTGAACTTGGCTCATCGACA / CTTGGACACAGGAGGAGAGC / 0.091 / 0.237 / 0.236 / 0.208
S636_E1 / Le_N7_S636 / 62898 / 63167 / 270 / AAGCAGTCTGGACAGGGAGA / GATATCAAGGCCGAAATCCA / 0.244 / 0.436 / 0.433 / 0.339
S139_E1 / Le_N7_S139 / 58087 / 58323 / 237 / GAGAGGAGCTCACTGCCATC / CTTCCGCTCTGTCAAGTTCC / 0.159 / 0.444 / 0.442 / 0.344
S235_E1 / Le_N7_S235 / 67599 / 67839 / 241 / AAGAGGAACATCATGACCGC / TTTCCTTGGACCGAGCATAC / 0.023 / 0.023 / 0.022 / 0.022
S160_E1 / Le_N7_S160 / 20605 / 20887 / 283 / GAGCTTCGGCTGTGAGTCTT / CGCAGCATGACTGAAAAATG / 0.437 / 0.717 / 0.713 / 0.661
S163_E1 / Le_N7_S163 / 62044 / 62225 / 182 / GGAAGCGAACAAACAAGCAT / GTCGTTGGCCGAAAAGTTTA / 0.224 / 0.656 / 0.652 / 0.588
S299_E1 / Le_N7_S299 / 61941 / 62164 / 224 / TACTGTTTTTGCAGCGCATC / CGTCAACGGTTTCAAGATCA / 0.341 / 0.399 / 0.397 / 0.359
S358_E1 / Le_N7_S358 / 26569 / 26844 / 276 / AGGAAGTTTTGGCTTGGGTT / CGTACCCTTGCTGGATCATT / 0.023 / 0.459 / 0.456 / 0.352
S413_E1 / Le_N7_S413 / 102632 / 102787 / 156 / CGCCATCAAGAGGGAAATTA / GACGCGTCGCCTATATTGAT / 0.110 / 0.323 / 0.321 / 0.270
S446_E1 / Le_N7_S446 / 47082 / 47313 / 232 / GAATCCTGCAGGTGTTCCAT / ACTGATTGTCCGGTTTCTCG / 0.034 / 0.214 / 0.213 / 0.194
S46_E1 / Le_N7_S46 / 21656 / 21876 / 221 / TGCCAGGAAGTCCCATCTAC / GGCTGCTACGGGATTCATAA / 0.250 / 0.246 / 0.245 / 0.231
S491_E1 / Le_N7_S491 / 40045 / 40266 / 222 / GTGCGTCTGTTCTCCTCCTC / CAAGCATCAGCAAAAACTGC / 0.013 / 0.061 / 0.061 / 0.060
S605_E1 / Le_N7_S605 / 35575 / 35802 / 228 / CACTGGTCATCAAATCTGCG / GCCATTAACGTAGTTGCCGT / 0.296 / 0.471 / 0.468 / 0.373
S767_E1 / Le_N7_S767 / 36226 / 36491 / 266 / TTCGAGCACTACACCGAATG / GGTACCACCAAACAATTGCC / 0.172 / 0.215 / 0.214 / 0.193
S81_E1 / Le_N7_S81 / 85127 / 85330 / 204 / CCTGGAGGGACAAATGAGAA / CCACGATCCTGAGACCAAAT / 0.024 / 0.091 / 0.091 / 0.087
S90_E1 / Le_N7_S90 / 66151 / 66392 / 242 / TTCGACGACTCAACGATGTC / GAGGAATTGAGGAGCAGACG / 0.102 / 0.099 / 0.098 / 0.095
S95_E1 / Le_N7_S95 / 20552 / 20846 / 295 / CGGACAATTTGGACTGGAAG / TGACTGGGACGTAATGGTGA / 0.216 / 0.331 / 0.329 / 0.291
S278_ID1 / Le_N7_S278 / 62317 / 62606 / 290 / TGGCTCGCTTTTCTTCTGAT / CGGCGGTAGTACCACAGAGT / 0.580 / 0.833 / 0.828 / 0.807
S127_ID1 / Le_N7_S127 / 25037 / 25281 / 245 / AAGAAACTGAACGCAAGGGA / TAGTACAGCCTTCCATCCCG / 0.437 / 0.478 / 0.475 / 0.428
S88_ID1 / Le_N7_S88 / 25075 / 25246 / 172 / TCTATCGAATCCGAGAACGG / GTGGACGTTGTGGAAGAGGT / 0.713 / 0.860 / 0.855 / 0.841
S150_ID1 / Le_N7_S150 / 765 / 983 / 219 / GACCAATCCAAAAATCGTGG / TTCCTCTGTCTGTGGCCTCT / 0.739 / 0.469 / 0.466 / 0.357
S113_ID1 / Le_N7_S113 / 92024 / 92307 / 284 / ACAGAATACAAGGCGGAACG / CCAATCGTGCGTATTTCCTT / 0.023 / 0.023 / 0.023 / 0.022
S250_ID1 / Le_N7_S250 / 55483 / 55780 / 298 / TGAAGACGAACAAGCTCCCT / CAACCAGTCCAACTTTCGGT / 0.214 / 0.200 / 0.199 / 0.192
S542_ID1 / Le_N7_S542 / 13136 / 13359 / 224 / GTGAGTATTGTGGGCGGACT / TTGCTCTGCTCATTGGTTTG / 0.624 / 0.637 / 0.634 / 0.595
S412_ID1 / Le_N7_S412 / 23933 / 24228 / 296 / AGACTCCAGTACGCGACGTT / GAAAGAATCCGGAGTGACGA / 0.427 / 0.575 / 0.571 / 0.486
S442_ID1 / Le_N7_S442 / 30722 / 30942 / 221 / GGCGTTGTATTGCTTCCAGT / CAGTGTGACGGACTCCAGAA / 0.221 / 0.430 / 0.427 / 0.379
S108_ID1 / Le_N7_S108 / 4050 / 4295 / 246 / TGATGATGGAGAAACACAAACC / ACCTGTTGCACAGGGGTATC / 0.513 / 0.785 / 0.780 / 0.752
S258_ID1 / Le_N7_S258 / 42453 / 42682 / 230 / ATGCACAATTGGCACACATC / TTAATCGCCCAAATACGAGC / 0.347 / 0.401 / 0.399 / 0.325
S260_ID1 / Le_N7_S260 / 69336 / 69602 / 267 / ATTCGAACGACCCGTATCAG / ACGTTGAAACCAACCGCTAC / 0.265 / 0.670 / 0.666 / 0.614
S476_ID1 / Le_N7_S476 / 194089 / 194386 / 298 / AGTGAGCGGCTACGAGTCAT / ATTTTGTGCAACAGCAGCAG / 0.423 / 0.788 / 0.783 / 0.750
S535_ID1 / Le_N7_S535 / 32684 / 32862 / 179 / AGAATCCACAGGCATTTTGC / TCCAAGGCCCAGATACATTC / 0.330 / 0.406 / 0.404 / 0.365
S603_ID1 / Le_N7_S603 / 126930 / 127208 / 279 / CAGTGTTATGATGTTCGCCG / GGCGTGTATGGTCGTCTTTT / 0.518 / 0.849 / 0.844 / 0.826
S267_ID1 / Le_N7_S267 / 14511 / 14800 / 290 / TTTTCCCTCCAATGAAGTCG / TTTTCGACATTCATCCAGCA / 0.511 / 0.712 / 0.708 / 0.670
S649_ID1 / Le_N7_S649 / 35018 / 35271 / 254 / TGGGTCTGGAGAACGACTTC / ACTCCAGAATTCCATCGTCG / 0.183 / 0.512 / 0.509 / 0.470
S255_ID1 / Le_N7_S255 / 22691 / 22987 / 297 / GTCGGAGATTCGTCCACAGT / ATCTTCATGGACGCGAAAAG / 0.570 / 0.728 / 0.724 / 0.683
S398_SSR1 / Le_N7_S398 / 29095 / 29300 / 206 / GCACTGGTGCCTTCCCCACT / TCGGATTGGAACCCGGAAGA / 0.500 / 0.678 / 0.675 / 0.618
S753_SSR1 / Le_N7_S753 / 108680 / 108858 / 179 / AGGCATAGCATGCAGCAGCG / AGGCTTAGGCGCAAACGCAG / 0.193 / 0.373 / 0.371 / 0.324
mean / 0.293 / 0.438 / 0.435 / 0.395

Notes: The scaffold number and the primer positions are derived from the L. edodes draft genome (unpublished data in Prof. Kwan's lab).

Ho, observed heterozygosity; He, expected heterozygosity; H, gene diversity; PIC, polymorphism information content.