Table S2. Analysis of posttranslational modification motifs in allergenic PME proteins.

The most representative and important posttranslational modifications were examined for the amino acid sequences of allergenic PME proteins from Olea europaea L., Salsola kali, Actinidia deliciosa, and Solanum lycopersicum. Functional and regulatory motifs included N-myristoylation, amidation, N-glycosilation, and phosphorylation by different protein kinases, i.e. caseine kinase I and II (CKI and II), Protein Kinase A (PKA), Protein Kinase C (PKC), Protein Kinase G (PKG), cyclin-dependent kinase 5 (Cdk5), cell division cycle 2 protein (cdc2), tyrosine-protein kinase Src, and P38 mitogen-activated protein kinases (p38MAPK).

GeneBank Accession N° / Species / Allergen name / N-Myristoylation / Amidation / N-Glycosilation / Phosphorylation and Kinase-Types
D8VPP5 / Olea europaea L. / Ole e 11-1 / 133GGtaAE138
140GTvdSA145
174GAqaAA179
224GSgkSI229
271GAflGR276
347GSieAS352 / 169dGKR172 / 103NYSE106 / CKII, PKA, PKC, PKG, cdc2, Src
B2VPR8 / Ole e 11-2
C1KET9 / Salsola kali / Sal k 1 / 26GLepSV31
37GGveTI42
115GTvdSA120
149GAqaSA154
244GTggTA249 / 144dGKR147 / 267NLSD270 / CKI, CKII, PKA, PKC, PKG, cdc2
Q17ST3 / Sal k 1 / 26GLepSV31
37GGveTI42
115GTvdSA120
149GAraSA154
244GTggTA249
Q17ST4 / Sal k 1
P85076 / Actinidia deliciosa / Act d 7 / 18GNftTV23
24GAavAA29
66GIgkTW71
121GAdfSA126
162GNaaAV167
273GSntTA278 / - / 19NFTT22
75NRSV78
275NTTA278
290NETE293 / CKI, CKII, PKA, PKC, PKG cdc2, Src
P09607 / Solanum lycopersicum / Lyc e PME / 158GVltNH163
294GMyaTI299
349GAdkSV354
501GAgtSK506 / - / 179NGTN182 / CKI, CKII, p38MAPK, cdk5, cdc2, PKA, PKC, PKG
Q96575 / Lyc e PME / 158GVltNH163
294GMhaTI299
349GAdkSV354
501GAgtSK506 / - / 179NGTN182 / CKI, CKII, p38MAPK, cdk5, cdc2, PKA, PKC, PKG
Q96576 / Lyc e PME / 153GVltNH158
289GMnaTI294
343GAdmSV348
495GAgtSK500
528GSwlNS533 / - / 174NGTV177
257NKTR260
291NATI294
532NSTS535 / CKI, CKII, p38MAPK, cdk5, cdc2, PKA, PKC, PKG
P14280 / Lyc e PME / 154GVltNH159
290GMyaTT295
345GAdmSV350
497GAgtSK502 / - / 175NGTN178 / CKII, cdc2, PKA, PKC, PKG