Supplementary Tables

Table S.1. Complete annotation of candidate genes for sting-time using the EAE backcross.

Bee
contig / Chromosome position (bp) / Fly
transcript homolog / Bee Genbank Gene ID / Gene Name / Function
14.24 / 8412555-8424204 / CG8581 / GB10232 / Frazzled / Molecular function: netrin receptor activity. There is experimental evidence that it is involved in the biological process: motor axon guidance; salivary gland boundary specification; axon guidance; dendrite morphogenesis; axon midline choice point recognition; dendrite guidance.
14.24 / 8425260-8426965 / CG6546 / GB14337 / Brahma associated protein 55kD / Predicted to have molecular function: structural constituent of cytoskeleton. There is experimental evidence that it is involved in the biological process: dendrite morphogenesis; mitosis; cytokinesis; muscle organ development; positive regulation of transcription, DNA-dependent.
14.24 / 8427841-8429758 / CG12697 / GB30160 / Odorant receptor 13a / Predicted to have molecular function: olfactory receptor activity.
14.24 / 8429859-8440620 / CG4252 / GB16077 / Meiotic 41 / Predicted to have molecular function: kinase activity. There is experimental evidence for 13 unique biological process terms, many of which group under: biological regulation; cell cycle; M phase of meiotic cell cycle; cellular macromolecule metabolic process; organelle organization; cell cycle process; signal transmission; nucleic acid metabolic process; regulation of cellular component organization; signaling process.
14.24 / 8441281-8442659 / CG9418 / GB17470 / Predicted to have molecular function: DNA binding.
14.24 / 8443030-8447293 / CG14803 / GB11053 / Molecular function is unknown.
14.24 / 8450224-8454750 / CG42734 / GB11433 / Ankyrin 2 / Predicted to have molecular function: structural constituent of cytoskeleton; cytoskeletal protein binding. There is experimental evidence that it is involved in the biological process: microtubule cytoskeleton organization; neuromuscular junction development; axon extension.
14.24 / 8459069-8467879 / CG4797 / GB13501 / Predicted to have molecular function: glucose transmembrane transporter activity.
14.24 / 8475292 8476185 / CG9762 / GB10658 / Lethal (3) neo18 / Predicted to have molecular function: NADH dehydrogenase activity; NADH dehydrogenase (ubiquinone) activity. There is experimental evidence that it is involved in the biological process: determination of adult lifespan; response to reactive oxygen species.
14.24 / 8486525-8488443 / CG30035 / GB19766 / Trehalose transporter 1-1 / Predicted to have molecular function: glucose transmembrane transporter activity. There is experimental evidence that it is involved in the biological process: trehalose transport.
14.24 / 8495296-8518655 / CG32435 / GB16396 / Chromosome bows / Experimental evidence that it has the molecular function: GTP binding; microtubule binding. There is experimental evidence for 16 unique biological process terms, many of which group under: cell cycle; cell cycle process; organelle organization; multicellular organism reproduction; gamete generation; nuclear division; regulation of cellular component organization; cellular component movement; fusome organization; regulation of developmental process; establishment or maintenance of cytoskeleton polarity.
14.24 / 8521760-8525579 / CG14408 / GB13029 / Predicted to have molecular function: SH3 domain binding.
14.24 / 8537465-8540743 / CG11804 / GB10510 / Ced-6 / Experimental evidence that it is involved in the biological process: mushroom body development.
14.24 / 8542804-8548123 / CG10997 / GB17906 / Chloride intracellular channel / Experimental evidence that it has the molecular function: calcium ion binding; chloride channel activity; lipid binding.
14.24 / 8556455-8560121 / CG7417 / GB18650 / TAK1-associated binding protein 2 / Reported to have molecular function: transcription factor activity. There is experimental evidence that it is involved in the biological process: response to bacterium; dendrite morphogenesis; antimicrobial humoral response.
14.24 / 8560214-8561184 / CG12343 / GB14256 / Reported to be involved in the biological process: nuclear mRNA splicing, via spliceosome.
14.24 / 8566191-8570693 / CG18255 / GB20023 / Stretchin-Mlck / Experimental evidence that it has the molecular function: myosin light chain kinase activity; calmodulin-dependent protein kinase activity. Based on sequence similarity, it is predicted to be involved in the biological process: protein amino acid phosphorylation.
14.24 / 8561336-8564572 / CG15040 / GB10449 / Molecular function is unknown.
14.24 / 8572391-8588409 / CG5235 / GB13817 / Predicted to have molecular function: dopamine beta-monooxygenase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: oxidation reduction; histidine catabolic process; catecholamine metabolic process.
14.24 / 8592996-8596756 / CG6227 / GB15485 / Predicted to have molecular function: mRNA binding; ATP-dependent RNA helicase activity; RNA helicase activity. There is experimental evidence that it is involved in the biological process: regulation of alternative nuclear mRNA splicing, via spliceosome.
14.24 / 8597638-8608329 / CG5392 / GB18088 / Reversion-inducing-cysteine-rich protein with kazal motifs / Predicted to have molecular function: serine-type endopeptidase inhibitor activity.
14.24 / 8613753-8614877 / CG1449 / GB13640 / Zn finger homeodomain 2 / Predicted to have molecular function: RNA polymerase II transcription factor activity. There is experimental evidence that it is involved in the biological process: nervous system development; imaginal disc-derived wing morphogenesis.
14.24 / 8615558-8616786 / CG30157 / GB16490 / Predicted to have molecular function: thiolester hydrolase activity.
14.24 / 8618828-8619259 / CG9065 / GB15238 / Molecular function: copper chaperone activity. Based on sequence similarity, it is predicted to be involved in the biological process: respiratory chain complex IV assembly.
14.24 / 8627982-8629758 / CG6415 / GB12854 / Predicted to have molecular function: aminomethyltransferase activity. Based on sequence similarity, it is predicted to be involved in the biological process: glycine catabolic process.
14.24 / 8655772-8689932 / CG2052 / GB12313 / It has the molecular function: zinc ion binding; nucleic acid binding.
14.24 / 8859309-8861111 / CG31689 / GB11259 / Predicted to have molecular function: ATPase activity, coupled to transmembrane movement of substances; transporter activity.
14.24 / 8867879-8919255 / CG5102 / GB19677 / daughterless / Experimental evidence that it has the molecular function: protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription regulator activity. There is experimental evidence that it is involved in the biological process: bristle morphogenesis; oogenesis; sensory organ development; response to DNA damage stimulus.
14.24 / 8947652-8949203 / CG32638 / GB17962 / Its molecular function is unknown. The biological processes in which it is involved are not known.
14.24 / 8949921-8952584 / CG3267 / GB19981 / Molecular function: methylcrotonoyl-CoA carboxylase activity; CoA carboxylase activity. There is experimental evidence that it is involved in the biological process: regulation of eclosion.
14.24 / 8953269-8958458 / CG13432 / GB13533 / Lethal (2) 05510 / Molecular function is unknown, the phenotypes of these alleles are annotated with: eye; trichogen cell; dorsocentral bristle; intersegmental nerve; tormogen cell; scutellar bristle; wing; ommatidium; larval central nervous system.
14.24 / 8971595-8976274 / CG8561 / GB20133 / Convoluted / Experimental evidence that it is involved in the biological process: motor axon guidance; regulation of tube size, open tracheal system; open tracheal system development; regulation of tube length, open tracheal system.
14.24 / 8982589-8987752 / CG4090 / GB13836 / Mucin related 89F / Molecular function: chitin binding.
14.24 / 8980563-8981250 / CG14959 / GB12661 / Cracked / Molecular function: chitin binding.
14.24 / 8998475-9000771 / CG12017 / GB13909 / Molecular function is unknown.
14.24 / 9005915-9006779 / CG14968 / GB17011 / Molecular function is unknown.
14.24 / 9012112-9015513 / CG13675 / GB19175 / Molecular function is unknown.
14.24 / 9025778-9033619 / CG13676 / GB17331 / Molecular function: chitin binding.
14.24 / 9039486-9041984 / GB17047 / Hypothetical protein.
14.24 / 9043059-9044428 / CG6980 / GB30549 / Molecular function: binding, Protein features are: Elongated TPR repeat-containing domain; Tetratricopeptide TPR-1; Tetratricopeptide repeat; Tetratricopeptide repeat-containing; Tetratricopeptide-like helical.
14.24 / 9044556-9045552 / CG42553 / GB30550 / Molecular function is unknown.
14.24 / 9045625-9047233 / CG1231 / GB18465 / Molecular function: zinc ion binding; nucleic acid binding.
14.24 / 9047633-9051093 / GB17581 / Hypothetical protein.
14.24 / 9202965-9235982 / CG1511 / GB12585 / Eph receptor tyrosine kinase / Predicted to have molecular function: ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity. There is experimental evidence that it is involved in the biological process: mushroom body development.
14.24 / 9241229-9242032 / CG6090 / GB14114 / Ribosomal protein L34a / Experimental evidence that it has the molecular function: protein binding, it is involved in the biological process: translation.
14.24 / 9242172-9245496 / GB19320 / 760 bp at 5' side: similar to Ribosomal protein L34a CG6090-PA, isoform A, 2696 bp at 3' side: similar to CG9300-PA.
14.24 / 9246189-9248594 / CG9300 / GB12978 / molecular function is unknown.
14.24 / 9248678-9251067 / CG4169 / GB19004 / Molecular function: ubiquinol-cytochrome-c reductase activity. Based on sequence similarity, it is predicted to be involved in the biological process: mitochondrial electron transport, ubiquinol to cytochrome c.
14.24 / 9253343-9259089 / CG9817 / GB10847 / Molecular function: zinc ion binding; nucleic acid binding.
14.24 / 9260093-9261335 / CG6030 / GB16485 / ATP synthase, subunit d.
14.24 / 9263416-9275674 / CG32743 / GB18026 / Smg1 / Reported to have molecular function: protein kinase activity. There is experimental evidence that it is involved in the biological process: nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.
14.24 / 9278033-9285763 / CG4557 / GB10448 / predicted to have molecular function: transcription factor activity
14.24 / 9287107-9287880 / CG42648 / GB13378 / Mitochondrial ribosomal protein S34 / Predicted to have molecular function: structural constituent of ribosome.
14.24 / 9288984-9292930 / CG4998 / GB17345 / Molecular function: serine-type endopeptidase activity.
14.24 / 9288984-9292930 / CG4795 / GB15220 / Calphotin / Experimental evidence that it has the molecular function: calcium ion binding.
14.24 / 9310871-9311569 / CG13055 / GB14187 / Molecular function is unknown.
14.24 / 9316473-9323422 / CG10737 / GB16444 / Predicted to have molecular function: diacylglycerol binding.
14.24 / 9330053-9335264 / CG15112 / GB17061 / Enabled / Experimental evidence that it has the molecular function: protein binding; SH3 domain binding.
14.24 / 9336178-9345290 / CG8887 / GB12658 / Absent, small, or homeotic discs 1 / Experimental evidence that it has the molecular function: histone-lysine N-methyltransferase activity; single-stranded RNA binding; histone methyltransferase activity (H3-K4 specific); histone methyltransferase activity. There is experimental evidence that it is involved in the biological process: histone methylation; oviposition; embryonic development via the syncytial blastoderm; chromatin-mediated maintenance of transcription; oogenesis; regulation of transcription.
14.24 / 9347978-9354008 / CG30122 / GB14495 / Predicted to have molecular function: mRNA binding. It is reported to be involved in the biological process: nuclear mRNA splicing, via spliceosome.
14.24 / 9354636-9357248 / CG12314 / GB13061 / Zucchini / Experimental evidence that it is involved in the biological process: gene silencing by RNA; oogenesis; dorsal appendage formation; karyosome formation.
14.24 / 9359931-9361526 / CG11221 / GB14793 / Predicted to have molecular function: protein serine/threonine kinase activity. It is reported to be involved in the biological process: protein amino acid phosphorylation.
14.24 / 9368187-9368922 / GB12718 / Hypothetical protein similar to beat-IV CG10152-PA.
14.24 / 9400149-9415774 / CG10152 / GB17482 / Beat-IV / Protein features are: Immunoglobulin-like.
14.24 / 9444126-9446118 / CG5279 / GB13493 / Rh5 / Experimental evidence that it has the molecular function: G-protein coupled photoreceptor activity. There is experimental evidence that it is involved in the biological process: phototransduction; absorption of visible light.
14.24 / 9589993-9655024 / CG10443 / GB15344 / Leukocyte-antigen-related-like / Experimental evidence that it has the molecular function: protein tyrosine phosphatase activity. There is experimental evidence for 11 unique biological process terms, many of which group under: anatomical structure development; cellular component movement; neuron differentiation; cell projection organization; cell morphogenesis; reproductive process in a multicellular organism; macromolecule modification; cell adhesion; regulation of developmental process; developmental growth; compound eye photoreceptor development; photoreceptor cell morphogenesis; protein modification process; multicellular organism reproduction; regulation of cell morphogenesis.
14.24 / 9660095-9661963 / CG5021 / GB16358 / Molecular function is unknown.
14.24 / 9662343-9677494 / CG13189 / GB12714 / Molecular function: metal ion transmembrane transporter activity, it is involved in the biological process: transmembrane transport; metal ion transport.
14.24 / 9707988-9713499 / ?CG15552 / GB14790 / Sox100B / Predicted to have molecular function: transcription factor activity; DNA bending activity. There is experimental evidence that it is involved in the biological process: male gonad development.
14.24 / 9718015-9722292 / CG15552 / GB19951 / Sox100B / Predicted to have molecular function: transcription factor activity; DNA bending activity. There is experimental evidence that it is involved in the biological process: male gonad development.
14.24 / 9722530-9725415 / CG6718 / GB19826 / Calcium-independent phospholipase A2 VIA / Predicted to have molecular function: calcium-independent phospholipase A2 activity.
14.24 / 9725963-9726796 / CG13415 / GB10864 / Chibby / Experimental evidence that it is involved in the biological process: negative regulation of Wnt receptor signaling pathway; segment polarity determination.
14.24 / 9726842-9730819 / CG10123 / GB12147 / Topoisomerase 3α / Experimental evidence that it has the molecular function: DNA topoisomerase activity, it is involved in the biological process: double-strand break repair via homologous recombination.
14.24 / 9731235-9735277 / CG1317 / GB15058 / It has the molecular function: zinc ion binding.
14.24 / 9735790-9739811 / CG16940 / GB12193 / Predicted to have molecular function: exoribonuclease II activity.
14.24 / 9740583-9745565 / CG5320 / GB15079 / Glutamate dehydrogenase / Experimental evidence that it has the molecular function: glutamate dehydrogenase [NAD(P)+] activity; glutamate dehydrogenase activity; identical protein binding. There is experimental evidence that it is involved in the biological process: NADH oxidation; aerobic respiration; glutamate metabolic process.
14.24 / 9747653-9751518 / CG42540 / GB11324 / Molecular function is unknown.
14.24 / 9753337-9755288 / CG13310 / GB15678 / Molecular function is unknown.
14.24 / 9763175-9770519 / CG8567 / GB19953 / Deformed epidermal autoregulatory factor-1 / It has the molecular function: transcription factor activity; sequence-specific DNA binding; RNA polymerase II transcription factor activity.
14.24 / 9789974 9793846 / CG9565 / GB17412 / Neprilysin 3 / Predicted to have molecular function: metallopeptidase activity; metalloendopeptidase activity.
14.24 / 9812222-9813372 / GB30542 / SATL1 (spermidine/spermine N1-acetyl transferase-like 1.

Table S.2. Complete annotation of candidate genes for sting-time using the EAA backcross.

Bee contig / Chromosome position (bp) / Fly