SUPPLEMENT DATA

18S rDNA gene sequencesforDrosophilamelanogasteradultflies

dimensionsnchar= 656;

formatmissing=? gap=- matchchar=. datatype=nucleotideinterleave

10ppb1day CAT GCA TGT CTA AGT ACA CAC GAT TAA CGT GAA ACC GCA AAA GGC TCA TTA TAT CAG TTA TGG TTC CTT AGA TCG TTA

10ppb5days ...... -...... A...... -......

20ppb1day ...... -...... A...... -......

20ppb5days ...... -...... C A.. ... -.. ... -......

50ppb1day ...... A...... C......

50ppb5days ...... -...... A.. ... -.. ... C......

75ppb1day ...... -...... A...... C......

75ppb5days ...... -...... A...... C......

control1day ...... A...... -......

control5days ...... A...... -......

10ppb1day ACA GTT ACT TGG ATA ACT GTG GTA ATT CTA GAG CTA ATA CAT GCA ATT AAA ACA TGA ACC TTA TGG GAC ATG TGC TTT

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......

10ppb1day TAT TAG GCT AAA ACC AAG CGA TCG CAA GAT CGT TAT ATT GGT TGA ACT CTA GAT AAC ATG CAG ATC GTA TGG TCT TGT

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......

10ppb1day ACC GAC GAC AGA TCT TTC AAA TGT CTG CCC TAT CAA CTT TTG ATG GTA GTA TCT AGG ACT ACC ATG GTT GCA ACG GGT

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......

10ppb1day AAC GGG GAA TCA GGG TTC GAT TCC GGA GAG GGA GCC TGA GAA ACG GCT ACC ACA TCT AAG GAA GGC AGC AGG CGC GTA

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......

10ppb1day AAT TAC CCA CTC CCA GCT CGG GGA GGT AGT GAC GAA AAA TAA CAA TAC AGG ACT CAT ATC CGA GGC CCT GTA ATT GGA

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......

10ppb1day ATG AGT ACA CTT TAA ATC CTT TAA CAA GGA CCA ATT GGA GGG CAA GTC TGG TGC CAG CAG CCG CGG TAA TTC CAG CTC

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ...... T ...... C......

50ppb5days ......

75ppb1day ...... - ......

75ppb5days ...... C......

control1day ......

control5days ......

10ppb1day CAA TAG CGT ATA TTA AAG TTG TTG CGG TTA AAA CGT TCG TAG TTG AAC TTG TGC TTC ATA CGC GTA GTA CAA CTT ACG

10ppb5days ...... G ...... A

20ppb1day ...... G ...... A

20ppb5days ...... G ...... A

50ppb1day ...... G ......

50ppb5days ...... G ...... A

75ppb1day ...... G ...... A

75ppb5days ...... A

control1day ...... G ...... A

control5days ...... G ...... A

10ppb1day ATT GTG GTT AGT ACT ATA CCT TTA TGT ATG TA

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ...... A. C......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......

ITS2 rDNA gene sequencesforDrosophilamelanogasteradultflies

dimensionsnchar= 439;

formatmissing=? gap=- matchchar=. datatype=nucleotideinterleave

10ppb1day TTA ATT TTA TAG TGC TGC TTG GAC TAC ATA TGG TTG AGG GTT GTA AGA CTA TGC TAA TTA AGT TGT TTA TAA ATT TTT

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ...... A......

75ppb1day ......

75ppb5days ...... C......

control1day ......

control5days......

10ppb1day TAT AAG CAT ATG GTA TAT TAT TGG ATT AAA TAA TGA TTT TAT TCA TAA TAT TAA AAA AGA AAT GAA AAA CAT TAT CTC

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ...... G......

control1day ......

control5days ......

10ppb1day ACA TTT GAA TGT GAA AAA CGA AGA GAA ATA TTT TCT TTT TCA ATC AAA TAA TAC TGA GAA ATG TCT AGC ATA AAA AAT

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......

10ppb1day TGA AAT ATT TTT CAT CTA GAA TTG TCT CTT ATT AAT GAT TCG AAA AAAAAAAAA CTT GGT TAT GTT ATT ATT CTT CGT

10ppb5days ......

20ppb1day ......

20ppb5days ......

50ppb1day ...... C ..C ...

50ppb5days ...... C...... C ......

75ppb1day ......

75ppb5days ...... C.C ...... C ......

control1day ......

control5days ......

10ppb1day TGG TTC GTT AAA TGG ATA AAA AAT AAC TTT GCT TAC AAG AAC TAT GGG AAC TAT TTA TAA CGA ATT TAA TGG ATT GTT

10ppb5days ...... A ...... T. ..G ...

20ppb1day G...... G ...... A ...... T.A ...... T......

20ppb5days G...... G ...... T......

50ppb1day G.. .C...... A .C. ... T...... T...... T......

50ppb5days ...... G...... G ...... T. ..G ...

75ppb1day G...... G...... A .C. ... T...... C ......

75ppb5days ...... G...... A ...... T...... C ...... G ...

control1day G.. .C...... T......

control5days ...... T......

10ppb1day TTA TCA TTT ATA TAT AAA GAA TTT ATG GCA AAA TAT AGT TAT ATA TAC A

10ppb5days ......

20ppb1day ...... T......

20ppb5days ......

50ppb1day ......

50ppb5days ......

75ppb1day ......

75ppb5days ......

control1day ......

control5days ......