Ammonium Chloride

Antifade (P-phenylenediamine dihydrochloride)

Bovine Serum Albumin

CaseinHazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

Name of supervisor / Laura Machesky / Assessment Number* / 2036
Signature
Assessor / Craig Stovold and Jason King / Date of Assessment / 28/02/02

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

1 / LOCATION OF THE WORK ACTIVITY / 8th Floor labs
2 / PERSONS WHO MAY BE AT RISK
List names where possible / The entire lab
3 / ACTIVITY ASSESSED / Immunocytochemistry
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT** / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
Ammonium Chloride / 0.134g / Harmful,
Irritant / R22
HARMFUL IF SWALLOWED
R36
IRRITATING TO EYES
S22
DO NOT BREATHE DUST
Antifade (P-phenylenediamine dihydrochloride) / 0.1g / Toxic,
Irritant / R20/21
HARMFUL BY INHALATION AND IN CONTACT WITH SKIN
R25
TOXIC IF SWALLOWED
R43
MAY CAUSE SENSITIZATION BY SKIN CONTACT
R50/53
VERY TOXIC TO AQUATIC ORGANISMS, MAY CAUSE LONG TERM ADVERSE AFFECTS IN THE AQUATIC ENVIRONMENT
Bovine Serum Albumin / 0.1g / Harmful,
Irritant,
Corrosive, / R36/37/38
IRRITATING TO EYES RESPIRATORY SYSTEM AND SKIN
R41
RISK OF SERIOUS DAMAGE TO EYES
Casein / 0.1g / Non hazardous / N/A
Ethanol / 80% (20ml) / Irritant,
Toxic,
Harmful, Flammable / R11
HIGHLY FLAMMABLE
R20,22
HARMFUL BY INHALATION AND IF SWALLOWED
Methanol / 50mg / Toxic,
Flammable / R11
HIGHLY FLAMMABLE
R23/25
TOXIC BY INHALATION AND IF SWALLOWED
Mowiol / 2.4g / Irritant
Harmful / R20/21/22
HARMFUL BY INHALATION, IN CONTACT WITH SKIN AND IF SWALLOWED
R36/37/38
IRRITATING TO EYES RESPIRATORY SYSTEM, AND SKIN
Nitric Acid / 100% (20ml) / Harmful
Very Corrosive
Fuming
Flammable
Explosive
Ingestion or Inhalation may be fatal / R8
CONTACT WITH COMBUSTIBLE MATERIAL MAY CAUSE FIRE
R35
CAUSES SEVERE BURNS
Paraformaldehyde / 4g / Harmful,
Toxic
Irritant
Carcinogenic / R20/21/22/23
HARMFUL BY CONTACT WITH SKIN, INHALATION, ANDIF SWALLOWED. TOXIC BY INHALATION.
R42/43
MAY CAUSE SENSITIZATION BY SKIN CONTACT AND INHALATION
R45/46
MAY CAUSE CANCER/HERITABLE GENETIC DAMAGE
Phalloidin (fluorescent conjugants)
Fluorescent conjugants are non-hazardous / 1/500 dilution / Very Toxic / R 26/27/28
VERY TOXIC BY INHALATION, IN CONTACT WITH SKIN AND IF SWALLOWED.
Picric acid / 4g / Irritant / R1 EXPLOSIVE WHEN DRY
R4 FORMS VERY SENSITIVE EXPLOSIVE METALLIC COMPOUNDS.
Sodium Hydroxide / 20g / Corrosive, Harmful / R35
CAUSES SEVERE BURNS
Tris-Cl / 0.1g / Irritant / R36/37/38
IRRITATING TO EYES RESPIRATORY SYSTEM AND SKIN
Triton X-100 / 1g / Harmful,
Damaging to eyes / R22
HARMFUL IF SWALLOWED
R41
RISK OF SERIOUS DAMAGE TO EYES
Primary antibodies (mouse, rat, rabbit) / 1/250+ dilution / Non-Hazardous / N/A
Secondary antibodies (fluorescent conjugate of µ-rabbit, mouse etc) / 1/250+ dilution / Non-Hazardous / N/A
Phosphate buffered saline / Non Hazardous / N/A
5 / INTENDED USE
Give brief details and attach protocol/instructions / PREPARATION OF COVERSLIPS,FIXING AND STAINING OF
CELLS FOR IMMUNOFLUORESCENT MICROSCOPY, PREPARATION OF SLIDES, PREPARATION OF
REAGENTS FOR FIXING, STAINING, AND PREPARING SLIDES AND COVERSLIPS. FOR TISSUE CULTURE HAZARDS PLEASE REFER TO TISSUE CULTURE COSHH FORM
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
RISK OF EXPOSURE TO NITRIC ACID AND NITRIC ACID FUMES DURING COVERSLIP PREPARATION
RISK OF EXPOSURE TO PARAFORMALDEHYDE DURING PREPARATION OF SOLUTION AND DURING FIXATION OF CELLS
RISK OF EXPOSURE TO MOWIOL/ANTIFADE DURING PREPARATION OF SOLUTION, AND DURING PREPARATION OF SLIDES
RISK OF EXPOSURE TO PHALLOIDIN AND METHANOL DURING PREPARATION OF SOLUTION AND DURING STAINING OF CELLS
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
RISKS ARE NOT ACCEPTABLE AND CONTROL MEASURES ARE NECESSARY
8 / CONTROL MEASURES
Additional to Good Chemical Practice
PERFORM COVERSLIP PREPARATION IN A FUME HOOD (Nitric acid), WHILST WEARING PROTECTIVE CLOTHING (lab coat, goggles)
WEAR GLOVES AT ALL TIMES WHEN FIXING AND STAINING CELLS, AS WELL AS WHEN PREPARING SLIDES (Exposure to paraformaldehyde, mowiol, antifade). LABEL TISSUE CULTURE PLATES AND WELLS AS CONTAINING PARAFORMALDEHYDE.
WEAR LAB COAT, GLOVES AND EYE PROTECTION WHILST PREPARING MOWIOL, ANTIFADE, PARAFORMALDEHYDE, AND PHALLOIDIN
APPROPRIATELY LABEL WATER BATHS WHILST INCUBATING MOWIOL/ANTIFADE, AND PARAFORMALDEHYDE FOR DISSOLVING.
ALIQUOT PREPARED SOLUTIONS OF 4% PARAFORMALDEYDE INTO 10ml, MOWIOL /ANTIFADE INTO 20ml AND PHALLOIDIN INTO 150ml TO PREVENT DEGRADATION AND TO MINIMIZE THE LEVEL OF EXPOSURE. LABEL TUBES CLEARLY WITH THEIR CONTENTS.
DISSOLVE PHALLOIDIN IN METHANOL IN A FUME HOOD
SOLVENTS KEPT IN A SEALED GLASS BOTTLE IN A FIREPROOF, LOCKED CABINET AWAY FROM SOURCES OF IGNITION
ACIDS STORED AT ROOM TEMPERATURE IN SEALED BOTTLES IN A CLEARLY LABELLED CABINET
PICRIC ACID: PROTECT FROM SOURCES OF IGINITION. DO NOT ALLOW TO DRY OUT.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Personnel involved in the clean up procedure must be adequately trained and experienced in handling carcinogenic materials.
10 / MONITORING
Performance of control measures,
CHECK FUME HOOD IS WORKING BEFORE USE.
Personal exposure / Health Surveillance
11 / WASTE DISPOSAL PROCEDURE
UNLESS OTHERWISE STATED DISPOSE ALL LIQUIDS BY WASHING DOWN THE SINK WITH PLENTY OF WATER. PLACE ALL SOLIDS INTO A BIN FOR INCINERATION.
IN THE CASE OF LIVE CULTURES, ASPIRATE MEDIUM INTO A PRESEPT SOLUTION, WHICH CAN BE WASHED DOWN THE SINK WITH RUNNING WATER. PLACE THE CELLS INTO A BAG FOR AUTOCLAVING
ANTIFADE: DISPOSE OF MATERIAL AND CONTAINER IN YELLOW BAG FOR INCINERATION.
PARAFORMALDEHYDE: DISPOSE OF MATERIAL IN YELLOW BAG FOR INCINERATION.
BSA: DISPOSE OF MATERIAL IN YELLOW BAG FOR INCINERATION.
PICRIC ACID: DISPOSE OF USING THE SPECIAL WASTE PROCEDURE (CONTACT BRIAN FACER IN STORES)
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
FEBRUARY 2007 OR EARLIER IF MORE INFORMATION BECOMES AVAILABLE
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
FOR FLAMMABLE MATERIALS (METHANOL, ETHANOL) SHUT OFF ALL SOURCES OF IGNITION IN THE
ROOM AND. IN CASE OF FIRE, SOUND THE FIRE ALARM. USE FIRE BLANKET/ EXTINGUISHER IF SAFE TO DO SO. CONSULT THE FIRE MARSHAL.
KEEP PERSONNEL NOT INVOLVED IN CLEAN UP OUT OF DANGER AREA.
PERSONNEL INVOLVED IN CLEAN UP MUST BE ADEQUATELY TRAINED AND EXPERIENCED.
WEAR ADEQUATE PERSONAL PROTECTIVE EQUIPMENT.
PREVENT MATERIAL FROM ENTERING DRAINS AND WATER COURSES.
STOP OR REDUCE LEAK IF SAFE TO DO SO.
TRANSFER CONTENTS FROM AN UNCONTROLLABLY LEAKING CONTAINER TO OTHER SUITABLE LABELLED CONTAINER IF SAFE TO DO SO.
AVOID GENERATING DUST, USE VACUUM CLEANER EQUIPPED WITH HEPA FILTER(S). ALTERNATIVELY, DAMPEN SPILLED MATERIAL WITH WATER.
SCOOP UP SPILL AND PLACE IN A SUITABLE, LABELLED CONTAINER.
THOROUGHLY WASH THE CONTAMINATED AREA WITH LOTS OF WATER (USE DISPERSING AGENT IF MATERIAL IMMISCIBLE WITH WATER).
FOR AIR CONTAMINATION EVACUATE THE ROOM AND VENTILATE ROOM AS MUCH AS POSSIBLE
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
KEEP PERSONNEL NOT INVOLVED IN CLEAN UP OUT OF DANGER AREA.
PERSONNEL INVOLVED IN CLEAN UP MUST BE ADEQUATELY TRAINED AND EXPERIENCED.
WEAR ADEQUATE PERSONAL PROTECTIVE EQUIPMENT
.
IF NECESSARYTHE INJURED PERSON SHOULD BE BROUGHT UNDER A SHOWER OR EYE WASHER AND THOROUGHLY WASH THE CONTAMINATED AREA , CONTAMINATED CLOTHING SHOULD BE REMOVED
IN THE EVENT OF INGESTION OF A TOXIC SUBSTANCE, REMOVE PEOPLE FROM THE SOURCE OF THE
CONTAMINATION, ALARM SECURITY FOR MEDICAL ATTENTION IMMEDIATELY, SHOWING THE LABEL OR CONTAINER WHERE POSSIBLE. DO NOT INDUCE VOMITING
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
REMOVE AND CLEAN UP HARMFUL LIQUIDS OR POWDERS WHILST WEARING APPROPRIATE PROTECTIVE CLOTHING E.G. GLOVES, MASK, GOGGLES.
THOROUGHLY WASH THE AREA WITH LOTS OF CLEAN WATER AND DETERGENT AND RINSE WITH LARGE QUANTITIES OF WATER (USE DISPERSING AGENT IF MATERIAL IMMISCIBLE WITH WATER).
CONTACT PHONE

10.10.00

* Prefix T is used for Teaching Assessment Number.

** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =

4g).

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.