Supplementary Information

Metabolomic investigations of Ricinus communis for cultivar and provenance determination

Eloise J. Pigott, Warren Roberts, Simon P. B. Ovenden, Simone Rochfort, David J. Bourne

E. J. Pigott, W. Roberts, S. P. B. Ovenden, D. J. Bourne

Defence Science and Technology Organisation, 506 Lorimer Street Fishermans Bend, Victoria, 3207, Australia

S. Rochfort

Victorian Department of Primary Industry, Victorian AgriBiosciences Centre, 1 Park Drive, Bundoora, Victoria, 3083, Australia, and The Faculty of Science, Technology and Engineering, La Trobe University, Bundoora, Victoria, 3086, Australia

Author of correspondence: Simon Ovenden, Defence Science and Technology Organisation, 506 Lorimer Street Fishermans Bend, Victoria, 3207, Australia.

Ph: +61 3 9626 8065, Fax: +61 3 9626 8410, email:


Figure S1: (a) OPLS-DA model scores plot classifying specimens according to their continent of origin; (NB: Spain and India are labelled by their country name rather than continent as there was only one specimen from their corresponding continents); (b) Loadings plot of LV1; (c) Loadings plot of LV2; (d) OPLS-DA scores plot of the first three latent variables (3D) separating African specimens according to their country of origin; (e) Loadings plot of LV1; (f) Loadings plot of LV2.

(a)

(b)

(c)

(d)

(e)

(f)


Figure S2: Loadings plots for cultivar classification OPLS-DA model generated in Figure 4a and 4b. (a) Loadings on LV1; (b) Loadings on LV2.

(a)

(b)


Table S1: Prediction table according to continent of origin correctly identifying all blinded samples

Obs ID / Spain / Africa / India
BS1 (DI) / 0.33 / -0.17 / 0.84
BS2 (GZ) / 0.05 / 1.02 / -0.07
BS4 (DI) / 0.24 / -0.23 / 0.99
BS5 (GZ) / -0.20 / 1.13 / 0.07
BS7 (DI) / -0.31 / -0.03 / 1.34
BS8 (GZ) / 0.25 / 0.72 / 0.03

Table S2: Prediction table according to African specimens correctly identifying the “gibsonii” Zimbabwe blinded samples.

Obs ID / ST / ZK / ZT / CT / IT / GZ
BS2 (GZ) / 0.21 / 0.07 / -0.01 / -0.12 / 0.01 / 0.84
BS5 (GZ) / -0.23 / -0.19 / 0.26 / 0.18 / 0.21 / 0.76
BS8 (GZ) / -0.05 / -0.20 / 0.22 / 0.02 / 0.05 / 0.97

SS: “sanguineus” Spain; ST: “sanguineus” Tanzania; ZK: “zanzibariensis” Kenya; ZT: “zanzibariensis” Tanzania; CT: “carmencita” Tanzania; IT: “impala” Tanzania; DI: “dehradun” India; GZ: “gibsonii” Zimbabwe

1