UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
Acetone cyanohydrin
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Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class
Purpose
Acetone cyanohydrin is an organic compound generated from nucleophilic attack of the carbonyl of acetone by cyanide. It is typically used in the production of methyl methacrylate, a monomer used to synthesize the plastic poly(methyl methacrylate) or PMMA. It is an extremely hazardous substance due to its tendency to readily decompose into acetone and hydrogen cyanide gas upon exposure to water or moisture.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 75-86-5
Class: Flammable liquid, acute toxicant
Molecular Formula: C4H7NO
Form (physical state): Liquid
Color: Clear to amber
Boiling point: 95 °C
Potential Hazards/Toxicity
Acetone cyanohydrin is a highly flammable liquid and vapor. Contact with acids liberates acutely toxic hydrogen cyanide gas. It is very toxic by inhalation, oral ingestion, and skin contact. Vapor inhalation may cause drowsiness and dizziness. Incompatible with heat, flames and sparks. It is harmful by oral ingestion, inhalation, and skin contact.
Engineering Controls
Work with this chemical in a certified ducted fume hood. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://www.ehs.ucsf.edu/respiratory-protection-program)
Hand Protection
Handle with nitrile or chloroprene gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with acetone cyanohydrin.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses or goggles. Face shield is also recommended.
Skin and Body Protection
Flame resistant lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hygiene Measures
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.
First Aid Procedures
If inhaled
Move person into fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Consult a physician.
In case of skin contact
Flush with plenty of water for at least 15 minutes while removing contaminated clothing. Take victim immediately to hospital.
In case of eye contact
Flush eyes with plenty of water for at least 15 minutes lifting upper and lower eyelids and removing contact lenses. Consult a physician. Continue rinsing eyes during transport to the hospital.
If swallowed
Never give anything by mouth to an unconscious person. Get medical aid immediately. Do NOT induce vomiting. If conscious and alert, rinse mouth with water.
Special Handling and Storage Requirements
Precautions for safe handling: Wear personal protective equipment. Ensure adequate ventilation. Avoid dust formation. Do not breathe dust. Do not get in eyes, on skin, or on clothing. Do not ingest. Keep away from clothing and other combustible materials.
Conditions for safe storage: Store in a flame-proof cabinet. Store in secondary containment with “acute toxin” labels on the primary container, secondary containment, and the storage location. Keep container tightly closed in a cool, dry, and well ventilated. Keep away from incompatible materials and conditions. Avoid strong acids, acid chlorides, acid anhydrides and chloroformates, and protect from sunlight.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885-7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://www.ehs.ucsf.edu/safety-data-sheet-sds-1
Protocol/Procedure
Quantities covered by this SOP:
______(g , ml) to ______(g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Acetone cyanohydrin 2 Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)