Manuscript No. FISH-D-16-00386

Fig S3: Multiple sequence alignment of C. catla CYP1A1 deduced amino acid sequence with other CYP1A1 sequences showing conserved domains and substrate recognition sites (SRS)
Catla / ---MALTILPLLGPISVSESLVAIITICVVYLLMRLMRTKIPEGLQKLPGPKPLPIIGNV / 57
Goldfish / ---MPLTVLPILGPISVSESLVAIITICVVYLLMRLMRTKIPEGLQKLPGPKPLPIIGNV / 57
Zebrafish / ---MALTILPILGPISVSESLVAIITICLVYLLMRLNRTKIPDGLQKLPGPKPLPIIGNV / 57
Atlanticsalmon / ---MVLMILPIIGSVSVSEGLVVMVTLCLVYMIMKYMHTEIPEGLKRLPGPKPLPIIGNV / 57
Rainbowtrout / ---MVLMILPIIGSVSASEGLVAMVTLCLVYMIMKYMHTEIPEGLKRLPGPKPLPIIGNM / 57
Redseabream / ------MILPFIGSVSVSESLVAMITMCLAYLILKFFRTEIPEGLRELPGPKPLPIIGNV / 54
Tigerperch / ---MALMIPPFIGSVSVSEGLVAMTTVCLIYLILKFFHTGIPEGLRRLPGPKPLPIIGSV / 57
Leapingmullet / ---MALMILPFIGALSVSESLVAVVTVCLVYLIIKSFQDKIPEGLSRLPGPKPLPIIGNV / 57
Largescalemullet / ---MALMILPFVGALSVSESLVAVVTVCLVYLIMKSFQEKIPEGLSRLPGPKPLPIIGNV / 57
Greymullet / ---MALMILPFIGALSVSESLVAVLTVCLAYLILKSFQDKIPEGLSRLPGPKPLPIIGNV / 57
Amursturgeon / ---MALISLPILGPILVSESLVAVATLCAVYLLLRVLRTEIPAGLRRPPGPTPLPLIGNV / 57
Africanclawedfrog / MTNWIGTVAGMMANTTITEFLVASVVFAIVFLVIRSQRVKIPPGTKKLPGPMPYPVIGNL / 60
Human / ------MLFPISMSATEFLLASVIFCLVFWVIRASRPQVPKGLKNPPGPWGWPLIGHM / 52
Housemouse / ----MPSMYGLPAFVSATELLLAVTVFCLGFWVVRATRTWVPKGLKTPPGPWGLPFIGHM / 56
Brownrat / ----MPSVYGFPAFTSATELLLAVTTFCLGFWVVRVTRTWVPKGLKSPPGPWGLPFMGHV / 56

: :* *:. .. : ::: : :* * *** *.:* :

Catla / LEVGNNPHLSLTAMSKCYGPVFQIQIGMRPVVVLSGNDVIRQALLKHGDEFAGRPDLHSS / 117
Goldfish / LEVGNNPHLSLTAMSKFYGPVFQIQIGMRPVVVLSGNDVIRQALLKQGEEFAGRPDLHSS / 117
Zebrafish / LEIGNNPHLSLTAMSKCYGPVFQIQIGMRPVVVLSGNDVIRQALLKQGEEFSGRPELYST / 117
Atlanticsalmon / LEVHNNPHLSLTAMSERYGSVFQIQIGMRPVVVLSGSETVRQALIKQGEDFAGRPDLYSF / 117
Rainbowtrout / LEVHNNPHLSLTAMSERYGSVFQIQIGMRPVVVLSGNETVRQALIKQGEDFAGRSDLYSF / 117
Redseabream / LEVGRNPYLSLTAMSKRYGDVFQIQIGMRPVVVLSGNETVRQAFIKQGEEFAGRPDLYSF / 114
Tigerperch / LEVGSRPYLSLTAMSKRYGDVFQIQIGMRPVVVLSGSDTVRQALIKQGEEFAGRPDLYSF / 117
Leapingmullet / LEVGSRPYLSLTEMGKRYGNVFQIQIGMRPVVVLSGNETVRQALIKQGDEFAGRPDLYSF / 117
Largescalemullet / LEMGSRPYLSLTEMSKRYGNVFQIQIGMRPVVVLSGNETVRQALIKQGDDFAGRPDLYSF / 117
Greymullet / LEMGSRPYLSLSEMSKRYGDVFQIQIGMRPVVVLSGNETVRQALIKQGDEFAGRPDLYSF / 117
Amursturgeon / PELGGNPHLSLTKMIQRYGDVTQIQIGTRPVVVLSGRETVRQALIKQGEDYAGRPDLNSI / 117
Africanclawedfrog / LSLSKNPHLSLTRMSETYGDVFQIQIGTKPVLVLSGLETLKQALIRQGDEFAGRPDLFTF / 120
Human / LTLGKNPHLALSRMSQQYGDVLQIRIGSTPVVVLSGLDTIRQALVRQGDDFKGRPDLYTF / 112
Housemouse / LTVGKNPHLSLTRLSQQYGDVLQIRIGSTPVVVLSGLNTIKQALVRQGDDFKGRPDLYSF / 116
Brownrat / LTLGKNPHLSLTKLSQQYGDVLQIRIGSTPVVVLSGLNTIKQALVKQGDDFKGRPDLYSF
: .*:*:*: : : ** * **:** **:**** :.::**::::*::: ** :* : / 116
Catla /
KFISDGKSLAFSTDKVGVWRARRKLALSALRTFSSVQGKSSEYSCALEEHISKEGLYLIE / 177
Goldfish / KFISDGKSLAFSTDQVGVWRARRKLALSALRTFSTVQAKSSEYSCALEEHISKEGLYLIE / 177
Zebrafish / KFISDGKSLAFSTDQVGVWRARRKLALNALRTFSTVQGKSPKYSCALEEHISNEGLYLVQ / 177
Atlanticsalmon / KFINDGKSLAFSTDKAGVWRARRKLAMSALRSFATLEGSTPEYSCALEEHVCKEGEYLVK / 177
Rainbowtrout / KFINDGKSLAFSTDKAGVWRARRKLAMSALRSFATLEGTTPEYSCALEEHVLKEGEYLVK / 177
Redseabream / KFINDGKSLAFSTDQAGVWRARRKLAYSALRSFATLEGTTPEYSCALEEHVSKEAEYLVK / 174
Tigerperch / RFINDGKSLAFSTDQAGVWRARRKLAYSALRSFSTLDSKTPEYSCMLEEHICKEGDYLIR / 177
Leapingmullet / RFISEGKSLAFSTDQAGVWRARRKLAYSALRSFSTLEGTTPEYSCVLEEHISKEAKYLIQ / 177
Largescalemullet / RFISEGKSLAFSTDQAGVWRARRKLAYSALRSFSTLEGTTPEYSCVLEEHISKEAEYLVK / 177
Greymullet / RFINDGKSLAFSTDKAGVWRARRKLAYSALRTFATLEGTTPEYSCVLEEHICKEADYPIK / 177
Amursturgeon / QFISNGKRMTFHNVHADVQRARRRPSPNALRSFSTVESPTSSYSCVLEEHISLEAEYLVK / 177
Africanclawedfrog / RMVGDGQSMTFSSDSGEVWRARRRLAQNALKTFATSPSPTSSSSCLVEENIITEAEYLVK / 180
Human / TLISNGQSMSFSPDSGPVWAARRRLAQNGLKSFSIASDPASSTSCYLEEHVSKEAEVLIS / 172
Housemouse / TLITNGKSMTFNPDSGPVWAARRRLAQNALKSFSIASDPTSASSCYLEEHVSKEANYLVS / 176
Brownrat / TLIANGQSMTFNPDSGPLWAARRRLAQNALKSFSIASDPTLASSCYLEEHVSKEAEYLIS / 176

:: :*: ::* : ***: : ..*::*: : ** :**.: *. :

Catla / RLHNVMKADGGFDPFRHIVVSVANVICGICFGRRYSHDDDELVSLVNMSDEFGKIVGSGN / 237
Goldfish / RLHSVMKADGSFDPFRHIVVSVANVICGICFGQRYSHDDDELVSLVNLSDEFGKIVGSGN / 237
Zebrafish / RLHSVMKADGSFDPFRHIVVSVANVICGICFGRRHSHDDDELVRLVNMSDEFGKIVGSGN / 237
Atlanticsalmon / QLTSVMDVSGSFDPFRHIVVSVANVICGMCFGRRYSHDDQELLGLVNLSDEFGQVVGSGN / 237
Rainbowtrout / QLTSVMDVSGSFDPFRHIVVSVANVICGMCFGRRYSHDDQELLGLVNMSDEFGQVVGSGN / 237
Redseabream / QFNNVMKADGSFDPFRHIVVSVANVICGMCFGRRYDYNNQELLDLVNLSDEFGQVVASGN / 234
Tigerperch / QLNTVMKADGSFDPFRHIVVSVANVICGMCFGRRYDHNDEELLSLVNLSDEFGQVVGSGN / 237
Leapingmullet / QLDTVMKADGSFDPFRYIVVSVANVICGMCFGRRYDHHDQELLSLVNLSDEFGQVVGSGN / 237
Largescalemullet / QLNTVMKADGSFDPFRYIVVSVANVICGMCFGRRYDHHDQELLSLVNLSDEFGQWWAVAT / 237
Greymullet / QLDSAMKADGSFDPFRYIVVSVANVICGMYFGRRYDHNDQELLGLVNLSDEFVQVVGSGN / 237
Amursturgeon / RLSGIMEADGSFDPFRHIVVSVANVICAMCFGRRYSHDDQELVNLVNLRDEFGRVIASGN / 237
Africanclawedfrog / KFMQLIDEKGEFDPYRYVVVSVANIICGMCFGKRYNHDDEELLNVVNLTDEFGAAAASGN / 240
Human / TLQELMAGPGHFNPYRYVVVSVTNVICAICFGRRYDHNHQELLSLVNLNNNFGEVVGSGN / 232
Housemouse / KLQKVMAEVGHFDPYKYLVVSVANVICAICFGQRYDHDDQELLSIVNLSNEFGEVTGSGY / 236
Brownrat / KFQKLMAEVGHFDPFKYLVVSVANVICAICFGRRYDHDDQELLSIVNLSNEFGEVTGSGY / 236

: : * *:*::::****:*:**.: **:*:.:..:**: :**: ::* . .

Catla / PADFIPFLRILPSTTMKKFVDI--NARFSNLMKKLVNEHYDTFNKDNIRDITDSLINLCE / 295
Goldfish / PADFIPFLRILPSTMMKKFVAI--NARFSKLMKKMVNDHYDTFNKDNIRDITDSLINHCE / 295
Zebrafish / PADFIPFLRILPSTTMKKFLDI--NERFSKFMKRLVMEHYDTFDKDNIRDITDSLINHCE / 295
Atlanticsalmon / PADFIPILRYLPNRTMKRFMDI--NDRFNTFVQKIVSEHYESYDKDNIRDITDSLIDHCE / 295
Rainbowtrout / PADFIPILRYLPNRTMKRFMDI--NDRFNNFVQKIVSEHYESYDKDNIRDITDSLIDHCE / 295
Redseabream / PADFIPILQYLPSTTMKKFLNISRNARFNEFVQKIVSEHYTTFDKDNIRDITDSLIDHCE / 294
Tigerperch / PADFIPVLQFLPSSTMKKFVSI--NDRFNKFVQKIVSEHYVTYDKDNIRDITDSLIDHCE / 295
Leapingmullet / PADFIPILQYLPNKTMKKFVSI--NDRFISFVQKIVSEHYATFNKDNIRDITDSLIDHCE / 295
Largescalemullet / QADFIPILQYLPNKTMKKFVNI--NGRFISFVQKIVNEHYATFNKDNIRDITDSLIDHCE / 295
Greymullet / PADFIPILQYLPNQTMKKFVGI--NDRFINFVQKIVTEHYATYNKDNIRDITDSLIDHCE / 295
Amursturgeon / PADFIPALRILPNRNMRAFIGI--SNRFNTFIQNIVTDHYRSFDKSNIRDITDSLIEHCQ / 295
Africanclawedfrog / PADFIPILQYLPSSSMKAFKEI--NRKFIDFMQKFATEHYKTFDKNHIRDITDSLIQHSQ / 298
Human / PADFIPILRYLPNPSLNAFKDL--NEKFYSFMQKMVKEHYKTFEKGHIRDITDSLIEHCQ / 290
Housemouse / PADFIPVLRYLPNSSLDAFKDL--NDKFYSFMKKLIKEHYRTFEKGHIRDITDSLIEHCQ / 294
Brownrat / PADFIPILRYLPNSSLDAFKDL--NKKFYSFMKKLIKEHYRTFEKGHIRDITDSLIEHCQ
***** *: **. : * : . :* :::.: :** :::* .*********: .: / 294
Catla /
DRKLDENSNIPVSDEKVVGIVNDLFGAGFDTISTALSWAVVYLVAYPEIQERLQRELKEK / 355
Goldfish / DRKLDENSNVQVSDEKIVGIVNDLFGAGFDTISTALSWGVVYLVAYPEIQERLQRELREK / 355
Zebrafish / DRKLDENSNLQVSDEKIVGIVNDLFGAGFDTISTALSWAVVYLVHYPEVQERLQRELDEK / 355
Atlanticsalmon / DRKLDENANVQVSDEKIVGIVNDLFGAGFDTISTALSWAVVYLVAYPEIQERLHQELKEK / 355
Rainbowtrout / DRKLDENANIQVSDEKIVGIVNDLFGAGFDTISTALSWAVVYLVAYPETQERLHQELKEK / 355
Redseabream / DRKLDENSNVQMSDEKIVGIVNDLFGAGFDTISTALSWSVMYLVAYPEIQERLYQEMKET / 354
Tigerperch / DRKLDENSNIQMSDEKIVGIVNDLFGAGFDTISTALSWSVMYLVAYPEIQERLYQELKDK / 355
Leapingmullet / DRKLDENANVQMSDEKVVGIVNDLFGAGFDTISTALSWSVMYLVAYPEIQEXLYQELKEN / 355
Largescalemullet / DRKLDENANVQMSDEKVVGIVNDLFGVGFDTISTALSWSVMYLVAYPEIQERLYQELKEN / 355
Greymullet / DRKLDENANVQISDEKVVGIVNDLFGAGFDTISTALSWSVMYLVTYPEIQERLYEELKEK / 355
Amursturgeon / DKKVDENANIQISDEKIVSIVNDLFGAGFDTISTALSWCLMYLVSHPDIQKKIHQVLDEH / 355
Africanclawedfrog / EKRVDENSNVQLSNQKIVNIVNDLFGAGFDTITTALSWSLMYLVAHPNIQEKIQDELDRV / 358
Human / EKQLDENANVQLSDEKIINIVLDLFGAGFDTVTTAISWSLMYLVMNPRVQRKIQEELDTV / 350
Housemouse / DRKLDENANVQLSDDKVITIVLDLFGAGFDTVTTAISWSLMYLVTNPRVQRKIQEELDTV / 354
Brownrat / DRRLDENANVQLSDDKVITIVFDLFGAGFDTITTAISWSLMYLVTNPRIQRKIQEELDTV / 354

::::***:*: :*::*:: ** ****.****::**:** ::*** * *. : :

Catla / VGMERTPRLSDRTDLPLLEAFILEIFRHSSFLPFTIPHCTTKDTSLNGYFIPKDTCVFVN / 415
Goldfish / IGMDRTPRLSDRTDLPLLEAFILEIFRHSSFLPFTIPHCTSKDTSLNGYFIPKDTCVFVN / 415
Zebrafish / IGKDRTPLLSDRANLPLLESFILEIFRHSSFLPFTIPHCTSKDTSLNGYFIPKDTCVFVN / 415
Atlanticsalmon / VGMTRTPRLSDKTNLPLLEAFILEIFRHSSFLPFTIPHCTVKDTSLNGYFIPKDTCVFIN / 415
Rainbowtrout / VGMIRTPRLSDKINLPLLEAFILEIFRHSSFLPFTIPHCTIKDTSLNGYFIPKDTCVFIN / 415
Redseabream / VGPDRMPCLSDKPNLPFLEAFILEILRHSSFLPFTIPHCTTKDTSLNGYFIPKDTCVFVN / 414
Tigerperch / VGLERSPRLSDKSNLPXLEAFILEIFRHSSFLPFTIPHCTSKDTSLNGYFIPKDTCVFIN / 415
Leapingmullet / VGLDRTPVLSDRNNLPLLESFILEIFRHSSFLPFTIPHCTTKDTSLNGYYIPKDTCVFIN / 415
Largescalemullet / VGLDRTPVLSDRNNLPLLEAFILEIFRHSSFLPFTIPHCTTKDTSLNGYHIPKDTCVFIN / 415
Greymullet / VGVDRTPVLADRNNLPLLEAFILEIFRHSSFLPFTIPHCTTKDTSLNGYFIPKDTCVFIN / 415
Amursturgeon / VGRERSPRLSDRPSLPYVEAFILETFRHSSFLPFTIPHCTTKDTALNGFYIPKDTCIFVN / 415
Africanclawedfrog / IGKERRPRLSDRAQLPYTEAFIFEMFRHSSFMPFTIPHCTTKDTVLNGYFIPKGICVLVN / 418
Human / IGRSRRPRLSDRSHLPYMEAFILETFRHSSFVPFTIPHSTTRDTSLKGFYIPKGRCVFVN / 410
Housemouse / IGRDRQPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTTRDTSLNGFYIPKGCCVFVN / 414
Brownrat / IGRDRQPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTIRDTSLNGFYIPKGHCVFVN
:* * * *:*: ** *:**:* :*****:******.* :** *:*:.*** *:::* / 414
Catla /
QWQVNRDSELWKDPSSFNPDRFLTADGTDLNRTEGEKVLTFGLGKRRCIGESIGRAEVFL / 475
Goldfish / QWQINHDPELWKDPSSFNLDRFLTADGTELNKIEGEKVLVFGLGKRRCIGESIGRAEVFL / 475
Zebrafish / QWQVNHDPELWKDPSSFIPDRFLTADGTELNKLEGEKVLVFGLGKRRCIGESIGRAEVFL / 475
Atlanticsalmon / QWQVNHDPELWKEPSSFNPDRFLSADGTELNKLEGEKVLVFGMGKRRCIGEAIGRNEVYL / 475
Rainbowtrout / QWQVNHDPELWKEPSSFNPDRFLSADGTELNKLEGEKVLVFGMGKRRCIGEAIGRNEVYL / 475
Redseabream / QWQINHDPELWKDPSSFNPDRFLNADGTEVNKLDGEKMMVFGMGKRRCIGEVIARNEVYL / 474
Tigerperch / QWQINHDPELWKDPSSFNPDRFLSADGTELKKLEGEKVMVFGMGKRRCIGEVIARNEVFL / 475
Leapingmullet / QWQINHDPELWKEPSSFNPDRFLSADGTEVNKVDGEKVMIFGMGKRRCIGEVIARNEVYL / 475
Largescalemullet / QWQINHDPELWKEPSSFNPDRFLSADGTEVNRVDGEKVMIFGMGKRRCIGEVIARNEVYM / 475
Greymullet / QWQINHDPELWKDPSSFNPDRFLNADGTEVNRVEGEKVVIFGMGKRRCIGEVIARNEVYM / 475
Amursturgeon / QWQVNHDPSLWKDPSTFSPERFLNAEGTGINKAESEKVMLFGLGKRRCIGESIGRSEVFL / 475
Africanclawedfrog / QWQVNHDPNLWKDPSKFYPERFLNTDGTMVNKTEMEKVMVFGLGKRRCVGEAIGRMEVFL / 478
Human / QWQINHDQKLWVNPSEFLPERFLTPDGAI-DKVLSEKVIIFGMGKRKCIGETIARWEVFL / 469
Housemouse / QWQVNHDRELWGDPNEFRPERFLTPSGTL-DKRLSEKVTLFGLGKRKCIGETIGRSEVFL / 473
Brownrat / QWQVNHDQELWGDPNEFRPERFLTSSGTL-DKHLSEKVILFGLGKRKCIGETIGRLEVFL / 473

***:*:* .** :*. * :***. .*: .: **: **:***:*:** *.* **::

Catla / FLAILLQRLKFSGMPGEMLDMTPEYGLTMKHKRCLLRATPQPGFEFPSSAA--- / 526
Goldfish / FMAILLQRLKFSGMPGEMLDMTPEYGLTMKHKRCMLRVTPQPGFELPGTAA--- / 526
Zebrafish / FLAILLQRLKFTGMPGEMLDMTPEYGLTMKHKRCLLRVTPQPGF------ / 519
Atlanticsalmon / FLAILLQRLCFQEKPGHPLDMTPEYGLTMKHKRCQLKASLRPWGQEE------ / 522
Rainbowtrout / FLAILLQRLRFQEKPGHPLDMTPEYGLTMKHKRCQLKASMRPWGQEE------ / 522
Redseabream / FLAILVQNLQFHAMPGEPLDMTPEYGLTMKHKRCHLRAAMRPRNEE------ / 520
Tigerperch / FLAIIVQKLHFHTMPGEPLDMTPEYGLTMKHKRCHLRATMRARDEQ------ / 521
Leapingmullet / FLAILIQKLHFCNLPGEPLDMTPEYGLTMKHKRCQLRATARVRSDH------ / 521
Largescalemullet / FLAILIQKLHFHNLPGEPLDMTPEYGLTMKHKRCQLRATARVRSDH------ / 521
Greymullet / FLAILIQNLNFYNLPGEPLDMTPEYGLTMKHKRCQLRATARVRDDY------ / 521
Amursturgeon / FLAVLLQRLGFRSLPGQKLDLTPEYGLTMKHKRCQLSASLLGRGSGCFLS---- / 525
Africanclawedfrog / FLTTMLQQMQFFKQDGEKLDMSPQYGLTMKHKRCHVTAKLRFPLLTTD------ / 526
Human / FLAILLQRVEFSVPLGVKVDMTPIYGLTMKHACCEHFQMQ---LRS------ / 512
Housemouse / FLAILLQQIEFKVSPGEKVDMTPTYGLTLKHARCEHFQVQ---MRSSGPQHLQA / 524
Brownrat / FLAILLQQMEFNVSPGEKVDMTPAYGLTLKHARCEHFQVQ---MRSSGPQHLQA / 524

*:: ::*.: * * :*::* ****:** *