CURRICULUM VITAE
Steven C. Almo, Ph.D.
Department of Biochemistry
Department of Physiology & Biophysics
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10461
phone: (718) 430-2746
fax: (718) 430-8565
e-mail:
Education
B.S. Biology, 1982 Massachusetts Institute of Technology
Cambridge, MA
Ph.D. Biophysics, 1990 Harvard University
Cambridge, MA
Post Doctoral,1992 Johns Hopkins School of Medicine
Cell Biology and Biophysics
Baltimore, MD
Positions/AWARDS
· Teaching Assistant, Stanford University Medical School, Dept. of Biochemistry, September 1982.
· Teaching Assistant, Harvard University, Dept. of Biochemistry and Molecular Biology, Sept. 1983-85.
· Recipient, Institute for Biological Recognition and Catalysis, Inc., Travel Grant, March 1988
· Recipient, Institute for Biological Recognition and Catalysis, Inc., Travel Grant, November 1988
· Instructor in Molecular Graphics Course, MIT, Department of Chemistry, 1988-1989.
· Instructor, Woods Hole Marine Biology Laboratories, Physiology Summer Course, Summer 1990.
· Assistant Professor of Biochemistry, Albert Einstein College of Medicine, Dept. of Biochemistry, March 1992-June 1997.
· Member, Albert Einstein Comprehensive Cancer Research Center, 1996-present
· Associate Professor of Biochemistry, Albert Einstein College of Medicine, Dept. of Biochemistry, July 1997-May 2001.
· Professor of Biochemistry, Albert Einstein College of Medicine, Dept. of Biochemistry, June 2001-present.
· Associate Director for Crystallography, Center for Synchrotron Biosciences, 1997-2006.
· Irma T. Hirschl/Monique Weill-Caulier Career Development Award, 2000-2004
· Professor of Physiology & Biophysics, Albert Einstein College of Medicine, Department of Physiology & Biophysics, June 2003-Present
· AMGEN AWARD, American Society for Biochemistry and Molecular Biology, 2004
· The LaDonne Schulman Faculty Recognition Award for Graduate Teaching at the Albert Einstein College of Medicine, 2005
· Director of Structural Proteomics, New York Structural Biology Center, November 2006-present
· Director, Albert Einstein Macromolecular Therapeutic Development Facility 2007-present
AECOM Service
· Faculty Senate, 1993-1995, 1998-2007
· Sue Golding Graduate Admissions Committee, 1993-1996
· MSTP Steering Committee, 1996-present
· Molecular Biophysics Training Grant Steering Committee, 1997-2010
· Associate Professor Promotion Committee, 1997-2000
· Advisory Committee, Analytical Imaging Facility, 2000-present
· Professor Promotion Committee, 2002-2004
· Tenure Committee, 2005-present
· Chair, Strategic Planning Committee on Structural Biology and Proteomics, 2007
National Service
· Special NCI Study Section for Program Project Review, 4/97
· American Cancer Society, Peer Review Committee on Cancer Drug Development, 1/98-12/01
· National Center for Research Resources Special Emphasis Panel ZRR1 BRT-1, April 1, 1998
· Special NCI Study Section for Program Project Review, 5/98
· NIH BBCB Study Section, Ad hoc, 7/98
· NIH Physical Biochemistry Study Section, Ad hoc, 6/00
· Editorial Board, International Archives of Allergy and Immunology, 1/98-12/00
· Proposal Study Panel, Brookhaven National Laboratory, 1999-present
· User Executive Committee, National Synchrotron Light Source, Brookhaven National Laboratory, 2001-2003, 2005-2007
· National Center for Research Resources Review Panel ZRG1 BBCA, October, 2001
· Reviewer for J. Biol. Chem., Biochemistry, Science, Nat. Struct. Biol., Biophys. J., Structure, Acta. Cryst, Chemistry & Biology, Nature, Proc. Natl. Acad. Sci., J. Cell Biol., Protein Science, Proteins
· Faculty of 1000, Electronic Reviews, Biology Reports Ltd.
· NIH CDF-4 Study Section, Ad hoc, 2/02
· NIH CSF (formerly CDF-4) Study Section, 10/02-10/06
· NIH Scientific Advisory Board, Epitope Discovery Working Group, NIAID, 9/04-12/05
· NIH Administrative Chairman of Division of Molecular and Cellular Mechanisms Special Emphasis Panel, 7/04-6/06
· NIH ZRG1-SBMI Review Panel, Ad hoc, 03/05
· Chairman, NIH ZRG1 CB-B 02 S, Review Panel (Bioengineering Research Partnership), 6/05
· NIH IDM-G (2) Review Panel, Ad hoc, 03/05
· NIH ZRG1 IDM-G (02) Drug Development 08/06
· NIH ZRG1 BST-D (50) Technology Centers for Networks and Pathways 3/09/09 – 3/10/09
· NIH CSF Study Section, Ad hoc, 6/09
· ZRG1 BCMB-B (99) R Review Panel 7/30/09
· Member, Beamline Advisory Team, National Synchrotron Light Source, 2009-present
· Scientific Advisory Committee, HIVRAD (Michael Cho, PI; Iowa State) 2009-present
· Scientific Advisory Committee, Center for Synchrotron Biosciences (Mark Chance, PI; Case Western Reserve) 2009-present
· Scientific Advisory Committee, PXRR (Robert Sweet, PI; Brookhaven National Laboratory) 2009-present; Chair 2011
· College of CSR Reviewers, 2010-present
· Organizer, 2012 Keystone Meeting “Structural Biology of Cellular Processes: From Atoms to Cells”
Professional Societies
· American Society for Cell Biology
· American Crystallographic Association
PUBLICATIONS:
1. Farber GK, Machin P, Almo SC, Petsko GA, Hajdu J. X-ray Laue diffraction from crystals of xylose isomerase. Proc Natl Acad Sci U S A. 1988;85(1):112-5. PMCID: 279493.
2. Mondragon A, Subbiah S, Almo SC, Drottar M, Harrison SC. Structure of the amino-terminal domain of phage 434 repressor at 2.0 A resolution. J Mol Biol. 1989;205(1):189-200.
3. Smith DL, Almo SC, Toney MD, Ringe D. 2.8-A-resolution crystal structure of an active-site mutant of aspartate aminotransferase from Escherichia coli. Biochemistry. 1989;28(20):8161-7.
4. Schlichting I, Almo SC, Rapp G, Wilson K, Petratos K, Lentfer A, Wittinghofer A, Kabsch W, Pai EF, Petsko GA, et al. Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis. Nature. 1990;345(6273):309-15.
5. Howell PL, Almo SC, Parsons MR, Hajdu J, Petsko GA. Structure determination of turkey egg-white lysozyme using Laue diffraction data. Acta Crystallogr B. 1992;48 ( Pt 2):200-7.
6. Almo SC, Pollard TD, Way M, Lattman EE. Purification, characterization and crystallization of Acanthamoeba profilin expressed in Escherichia coli. J Mol Biol. 1994;236(3):950-2.
7. Almo SC, Smith DL, Danishefsky AT, Ringe D. The structural basis for the altered substrate specificity of the R292D active site mutant of aspartate aminotransferase from E. coli. Protein Eng. 1994;7(3):405-12.
8. Fedorov AA, Magnus KA, Graupe MH, Lattman EE, Pollard TD, Almo SC. X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates. Proc Natl Acad Sci U S A. 1994;91(18):8636-40. PMCID: 44661.
9. Fedorov AA, Pollard TD, Almo SC. Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli. J Mol Biol. 1994;241(3):480-2.
10. Pollard TD, Almo S, Quirk S, Vinson V, Lattman EE. Structure of actin binding proteins: insights about function at atomic resolution. Annu Rev Cell Biol. 1994;10:207-49.
11. Fedorov AA, Joseph-McCarthy D, Fedorov E, Sirakova D, Graf I, Almo SC. Ionic interactions in crystalline bovine pancreatic ribonuclease A. Biochemistry. 1996;35(50):15962-79.
12. Joseph-McCarthy D, Fedorov AA, Almo SC. Comparison of experimental and computational functional group mapping of an RNase A structure: implications for computer-aided drug design. Protein Eng. 1996;9(9):773-80.
13. Wiedemann P, Giehl K, Almo SC, Fedorov AA, Girvin M, Steinberger P, Rudiger M, Ortner M, Sippl M, Dolecek C, Kraft D, Jockusch B, Valenta R. Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody. J Biol Chem. 1996;271(47):29915-21.
14. Fedorov AA, Ball T, Mahoney NM, Valenta R, Almo SC. The molecular basis for allergen cross-reactivity: crystal structure and IgE-epitope mapping of birch pollen profilin. Structure. 1997;5(1):33-45.
15. Fedorov AA, Ball T, Valenta R, Almo SC. X-ray crystal structures of birch pollen profilin and Phl p 2. Int Arch Allergy Immunol. 1997;113(1-3):109-13.
16. Fedorov AA, Lappalainen P, Fedorov EV, Drubin DG, Almo SC. Structure determination of yeast cofilin. Nat Struct Biol. 1997;4(5):366-9.
17. Goldsmith SC, Pokala N, Matsudaira P, Almo SC. Crystallization and preliminary crystallographic analysis of the N-terminal actin binding domain of human fimbrin. Proteins. 1997;28(3):452-3.
18. Goldsmith SC, Pokala N, Shen W, Fedorov AA, Matsudaira P, Almo SC. The structure of an actin-crosslinking domain from human fimbrin. Nat Struct Biol. 1997;4(9):708-12.
19. Lappalainen P, Fedorov EV, Fedorov AA, Almo SC, Drubin DG. Essential functions and actin-binding surfaces of yeast cofilin revealed by systematic mutagenesis. Embo J. 1997;16(18):5520-30. PMCID: 1170184.
20. Mahoney NM, Janmey PA, Almo SC. Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation. Nat Struct Biol. 1997;4(11):953-60.
21. Puius YA, Zhao Y, Sullivan M, Lawrence DS, Almo SC, Zhang ZY. Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: a paradigm for inhibitor design. Proc Natl Acad Sci U S A. 1997;94(25):13420-5. PMCID: 28320.
22. Degano M, Almo SC, Sacchettini JC, Schramm VL. Trypanosomal nucleoside hydrolase. A novel mechanism from the structure with a transition-state inhibitor. Biochemistry. 1998;37(18):6277-85.
23. Eads JC, Mahoney NM, Vorobiev S, Bresnick AR, Wen KK, Rubenstein PA, Haarer BK, Almo SC. Structure determination and characterization of Saccharomyces cerevisiae profilin. Biochemistry. 1998;37(32):11171-81.
24. Hanein D, Volkmann N, Goldsmith S, Michon AM, Lehman W, Craig R, DeRosier D, Almo S, Matsudaira P. An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. Nat Struct Biol. 1998;5(9):787-92.
25. Liu S, Fedorov AA, Pollard TD, Lattman EE, Almo SC, Magnus KA. Crystal packing induces a conformational change in profilin-I from Acanthamoeba castellanii. J Struct Biol. 1998;123(1):22-9.
26. Mahoney NM, Almo SC. Crystallization and preliminary X-ray analysis of human platelet profilin complexed with an oligo proline peptide. Acta Crystallogr D Biol Crystallogr. 1998;54(Pt 1):108-10.
27. Mao C, Cook WJ, Zhou M, Federov AA, Almo SC, Ealick SE. Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues. Biochemistry. 1998;37(20):7135-46.
28. Mitra J, Tang X, Almo SC, Shields D. Temperature-induced conformational changes in prosomatostatin-II: implications for processing. Biochem J. 1998;334 ( Pt 1):275-82. PMCID: 1219689.
29. Patskovska LN, Fedorov AA, Patskovsky YV, Almo SC, Listowsky I. Expression, crystallization and preliminary X-ray analysis of ligand-free human glutathione S-transferase M2-2. Acta Crystallogr D Biol Crystallogr. 1998;54(Pt 3):458-60.
30. Puius YA, Mahoney NM, Almo SC. The modular structure of actin-regulatory proteins. Curr Opin Cell Biol. 1998;10(1):23-34.
31. Puius YA, Zou M, Ho NT, Ho C, Almo SC. Novel water-mediated hydrogen bonds as the structural basis for the low oxygen affinity of the blood substitute candidate rHb(alpha 96Val-->Trp). Biochemistry. 1998;37(26):9258-65.
32. Valenta R, Almo S, Ball T, Dolecek C, Steinberger P, Laffer S, Eibensteiner P, Flicker S, Vrtala S, Spitzauer S, Valent P, Denepoux S, Kraft D, Banchereau J, Lebecque S. The immunoglobulin E-allergen interaction: a target for therapy of type I allergic diseases. Int Arch Allergy Immunol. 1998;116(3):167-76.
33. Burley SK, Almo SC, Bonanno JB, Capel M, Chance MR, Gaasterland T, Lin D, Sali A, Studier FW, Swaminathan S. Structural genomics: beyond the human genome project. Nat Genet. 1999;23(2):151-7.
34. Fedorov AA, Fedorov E, Gertler F, Almo SC. Structure of EVH1, a novel proline-rich ligand-binding module involved in cytoskeletal dynamics and neural function. Nat Struct Biol. 1999;6(7):661-5.
35. Levin S, Almo SC, Satir BH. Functional diversity of the phosphoglucomutase superfamily: structural implications. Protein Eng. 1999;12(9):737-46.
36. Mahoney NM, Rozwarski DA, Fedorov E, Fedorov AA, Almo SC. Profilin binds proline-rich ligands in two distinct amide backbone orientations. Nat Struct Biol. 1999;6(7):666-71.
37. Shi W, Li CM, Tyler PC, Furneaux RH, Cahill SM, Girvin ME, Grubmeyer C, Schramm VL, Almo SC. The 2.0 A structure of malarial purine phosphoribosyltransferase in complex with a transition-state analogue inhibitor. Biochemistry. 1999;38(31):9872-80.
38. Shi W, Li CM, Tyler PC, Furneaux RH, Grubmeyer C, Schramm VL, Almo SC. The 2.0 A structure of human hypoxanthine-guanine phosphoribosyltransferase in complex with a transition-state analog inhibitor. Nat Struct Biol. 1999;6(6):588-93.
39. Shi W, Schramm VL, Almo SC. Nucleoside hydrolase from Leishmania major. Cloning, expression, catalytic properties, transition state inhibitors, and the 2.5-a crystal structure. J Biol Chem. 1999;274(30):21114-20.
40. Apgar J, Tseng Y, Fedorov E, Herwig MB, Almo SC, Wirtz D. Multiple-particle tracking measurements of heterogeneities in solutions of actin filaments and actin bundles. Biophys J. 2000;79(2):1095-106. PMCID: 1301005.
41. Burke E, Mahoney NM, Almo SC, Barik S. Profilin is required for optimal actin-dependent transcription of respiratory syncytial virus genome RNA. J Virol. 2000;74(2):669-75. PMCID: 111586.
42. Melian A, Watts GF, Shamshiev A, De Libero G, Clatworthy A, Vincent M, Brenner MB, Behar S, Niazi K, Modlin RL, Almo S, Ostrov D, Nathenson SG, Porcelli SA. Molecular recognition of human CD1b antigen complexes: evidence for a common pattern of interaction with alpha beta TCRs. J Immunol. 2000;165(8):4494-504.
43. Ostrov DA, Shi W, Schwartz JC, Almo SC, Nathenson SG. Structure of murine CTLA-4 and its role in modulating T cell responsiveness. Science. 2000;290(5492):816-9.
44. Puius YA, Fedorov EV, Eichinger L, Schleicher M, Almo SC. Mapping the functional surface of domain 2 in the gelsolin superfamily. Biochemistry. 2000;39(18):5322-31.
45. Sarmiento M, Puius YA, Vetter SW, Keng YF, Wu L, Zhao Y, Lawrence DS, Almo SC, Zhang ZY. Structural basis of plasticity in protein tyrosine phosphatase 1B substrate recognition. Biochemistry. 2000;39(28):8171-9.
46. Shi W, Munagala NR, Wang CC, Li CM, Tyler PC, Furneaux RH, Grubmeyer C, Schramm VL, Almo SC. Crystal structures of Giardia lamblia guanine phosphoribosyltransferase at 1.75 A(,). Biochemistry. 2000;39(23):6781-90.
47. Wolven AK, Belmont LD, Mahoney NM, Almo SC, Drubin DG. In vivo importance of actin nucleotide exchange catalyzed by profilin. J Cell Biol. 2000;150(4):895-904. PMCID: 2175289.
48. Fedorov A, Shi W, Kicska G, Fedorov E, Tyler PC, Furneaux RH, Hanson JC, Gainsford GJ, Larese JZ, Schramm VL, Almo SC. Transition state structure of purine nucleoside phosphorylase and principles of atomic motion in enzymatic catalysis. Biochemistry. 2001;40(4):853-60.
49. Goldsmith SC, Guan JQ, Almo S, Chance M. Synchrotron protein footprinting: a technique to investigate protein-protein interactions. J Biomol Struct Dyn. 2001;19(3):405-18.
50. Rustandi R, Vallely KM, Varlamova O, Klein MG, Almo SC, Bresnick AR, Weber DJ. 1H, 13C, 15N NMR sequence-specific resonance assignments for human apo-Mts1 (S100A4). J Biomol NMR. 2001;19(4):381-2.
51. Schwartz JC, Zhang X, Fedorov AA, Nathenson SG, Almo SC. Structural basis for co-stimulation by the human CTLA-4/B7-2 complex. Nature. 2001;410(6828):604-8.
52. Shi W, Basso LA, Santos DS, Tyler PC, Furneaux RH, Blanchard JS, Almo SC, Schramm VL. Structures of purine nucleoside phosphorylase from Mycobacterium tuberculosis in complexes with immucillin-H and its pieces. Biochemistry. 2001;40(28):8204-15.
53. Shi W, Tanaka KS, Crother TR, Taylor MW, Almo SC, Schramm VL. Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae. Biochemistry. 2001;40(36):10800-9.
54. Tseng Y, Fedorov E, McCaffery JM, Almo SC, Wirtz D. Micromechanics and ultrastructure of actin filament networks crosslinked by human fascin: a comparison with alpha-actinin. J Mol Biol. 2001;310(2):351-66.
55. Zhang X, Schwartz JC, Nathenson SG, Almo SC. Crystallization and preliminary X-ray analysis of the complex between human CTLA-4 and B7-2. Acta Crystallogr D Biol Crystallogr. 2001;57(Pt 6):898-9.
56. Bobkov AA, Muhlrad A, Kokabi K, Vorobiev S, Almo SC, Reisler E. Structural effects of cofilin on longitudinal contacts in F-actin. J Mol Biol. 2002;323(4):739-50.
57. Bubb MR, Govindasamy L, Yarmola EG, Vorobiev SM, Almo SC, Somasundaram T, Chapman MS, Agbandje-McKenna M, McKenna R. Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution. J Biol Chem. 2002;277(23):20999-1006.
58. Chance MR, Bresnick AR, Burley SK, Jiang JS, Lima CD, Sali A, Almo SC, Bonanno JB, Buglino JA, Boulton S, Chen H, Eswar N, He G, Huang R, Ilyin V, McMahan L, Pieper U, Ray S, Vidal M, Wang LK. Structural genomics: a pipeline for providing structures for the biologist. Protein Sci. 2002;11(4):723-38. PMCID: 2373525.