BIOGRAPHICAL SKETCH

Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
Lynch, Kristen Wood / POSITION TITLE
Associate Professor
eRA COMMONS USER NAME
klynch
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION / DEGREE
(if applicable) / YEAR(s) / FIELD OF STUDY
Harvard University / BA / 1990 / Biochemistry
Harvard University / Ph.D. / 1996 / Biochemistry
University of California, San Francisco / 1997-2001 / Immunology

Please refer to the application instructions in order to complete sections A, B, and C of the Biographical Sketch.

A. Positions and Honors

 PHS 398/2590 (Rev. 05/01) Page ___7____ Biographical Sketch Format Page

Research Positions

1989-1990 Undergraduate Research

Laboratory of Prof. Mark Ptashne, Harvard University

1992-1996 Graduate Student

Laboratory of Prof. Tom Maniatis, Harvard University

1997-2001 Postdoctoral Fellow

Laboratory of Prof. Arthur Weiss, UC, San Francisco

2001-2007 Assistant Professor

Department of Biochemistry, UT Southwestern Medical Center

2007-2009 Associate Professor

Department of Biochemistry, UT Southwestern Medical Center

2009-present Associate Professor

Department of Biochemistry and Biophysics

University of Pennsylvania School of Medicine

Honors and Awards

1991-1994 National Science Foundation Pre-doctoral Fellowship

1999-2001 Cancer Research Institute Post-doctoral Fellowship

2001-2009 E.E. and Greer Garson Fogelson Scholar in Biomedical Research

UTSWMC Endowed Scholar Program

2004-2009 National Science Foundation Career Award

B. Peer-Reviewed Publications

Topp J.D., J. Jackson, A.A. Melton, K.W. Lynch (2008) A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4. RNA 14: 2038-2049.

House, A.E. and K.W. Lynch (2008) Regulation of alternative splicing: more than just the ABCs. J. Biol. Chem. 18: 1217-1221.

Melton, A.A., J. Jackson, J. Wang and K.W. Lynch. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol. Cell. Biol. 27:6972-6984.

Ip, J., A. Tong, Q. Pan, J. Topp, B.J. Blencowe* and K.W. Lynch*. (2007) Global analysis of

alternative splicing during T cell activation. RNA 13: 563-572. (* co-corresponding authors)

House, A.E. and K.W. Lynch. (2006) An Exonic Splicing Silencer Represses Spliceosome Assembly

after ATP-dependent Exon Recognition. Nat. Struct. Mol. Biol. 13: 937-944.

Johnson, E.B., D.J. Steffen, K. W. Lynch, and J. Herz. (2006) Defective splicing of MEGF7/LRP4,

a regulator of distal limb development, in autosomal recessive Mulefoot disease. Genomics 88: 600-609.

Levinson, N., R. Hinman, A. Patil, C.R.J. Stephenson, S. Werner, G.H.C. Woo, J. Xiao, P. Wipf and

K.W. Lynch (2006) Use of transcriptional synergy to augment sensitivity of a splicing reporter assay. RNA 12:925-930.

Tong, A., J. Nguyen and K.W. Lynch (2005) Differential expression of CD45 isoforms is controlled

by the combined activity of basal and inducible splicing regulatory elements in each of the variable exons. J. Biol. Chem. 280: 38297-304.

Rothrock, C.R.*, A.E. House* and K.W. Lynch (2005) HnRNP L represses exon splicing via a

regulated exonic splicing silencer. EMBO J 24: 2792-2802. (* co-first authors)

Lynch, K.W. (2004) Consequences of regulated pre-mRNA splicing in the immune system. Nat Rev

Immunol. 4: 931-940.

Rothrock, C., B. Cannon, B. Hahm and K.W. Lynch (2003) A conserved signal-responsive sequence

mediates activation-induced alternative splicing of CD45. Mol. Cell 12: 1317-1324.

Sheives, P. and K.W. Lynch (2002) Identification of cells deficient in signaling-induced alternative

splicing by use of somatic-cell genetics. RNA 8:1473-1481.

Lynch, K.W. and A. Weiss (2001) A CD45 polymorphism associated with multiple sclerosis disrupts an

exonic splicing silencer. J. Biol. Chem. 276: 24341-24347.

Lynch, K.W. and A. Weiss (2000) A model system for activation-induced alternative splicing of CD45

pre-mRNA in T cells implicates protein kinase C and Ras. Mol Cell Biol 20: 70-80.

Hertel, K.J., K.W. Lynch and T. Maniatis (1997) Common themes in the function of transcription and

splicing enhancers. Curr Opin Cell Biol. 9: 350-357.

Hertel, K.J., K.W. Lynch, E. C. Hsiao, E. H.-T. Liu and T. Maniatis (1996) Structural and functional

conservation of the Drosophila doublesex splicing enhancer repeat elements. RNA 2: 969-981.

Lynch, K.W. and T. Maniatis (1996) Assembly of specific SR protein complexes on distinct regulatory

elements of the Drosophila doublesex splicing enhancer. Genes & Dev. 10: 2089-2101.

Lynch, K.W. and T. Maniatis (1995) Synergistic interactions between two distinct elements of a

regulated splicing enhancer. Genes & Dev. 9: 284-293.

Sadowski, I., D. Neidbala, K. Wood and M. Ptashne (1991) Gal4 is phosphorylated as a consequence

of transcriptional activation. Proc. Natl. Acad. Sci.USA 88: 10510-10514.

Ruden, D.M., J. Ma, Y. Li, K. Wood and M. Ptashne (1991) Generating yeast transcriptional activators

containing no yeast protein sequences. Nature 350: 250-252.

C. Other Support

Current

NIH-NIGMS Role: PI

“Mechanisms of Signal-Induced Alternative Splicing: CD45”

Duration: 5/01/08-6/30/12

This project is focused on characterizing the cis-sequences, RNA binding proteins and signaling pathways that mediate signal-induced repression of CD45 exon 4.

NIH-NIGMS Role: PI

“Coordination of Inducible Alternative Splicing Networks in Human T cells”

Duration: 6/01/08-7/31/12

This project is focused on characterizing sequence motifs and trans-regulatory proteins that control co-regulation of alternative splicing within multiple genes in response to activation of cultured T cell lines.

Completed

NSF Role: PI

“CAREER: Characterization of novel targets of activation-induced alternative splicing”

Duration: 3/1/04-2/28/09

This project is focused on identifying new targets and networks of signal induced splicing regulation in T cells.

Welch Foundation Role: PI

“Structural Characterization of a Novel Transition in Spliceosome Assembly”

Duration: 6/01/06-05/31/09

This is a small foundation award which is funding pilot studies toward a structural characterization of the AEC complex.