15 points total Biol203 Fall 2013
Homework 9:
Also due in your lab starting Nov 13th and will be graded by your Lab TA.
Figure 1 represents 7 different versions of the Red/Green gene cluster in human males. They are all on the X chromosome.
1) This gene cluster represents a set of homologous genes called____paralogues____.
2) These genes are located on the X chromosome in man, if they were located on the 3rd chromosome of mouse (not X), then this gene cluster would be referred to as orthologues___ relative to the human cluster?
3) These genes are located on the X chromosome in man, if they were located on the X chromosome in mouse, then this gene cluster would be referred to as orthologues __ relative to the human cluster?
4) How many TSS sites are there for this gene cluster?___1_
5) How many different proteins (there is no alternate splicing between protein coding units) did the evolutionary ancestor originally make?_____ 1______
6) Based on the information given, which is (are) the ancestral protein?__A_
7) and why is (are) this (answer to #6) likely to be the ancestral protein?
______Because the TSS is at it’s 5’ end and a critical enhancer at its 3’ end ______
8) Assuming the proteins are 300aa, then where (approximately) are the residues located that give A its identity?____Between 100-150aa______
9) Assuming the proteins are 300aa, then where (approximately) are the residues located that give B its identity?_____ Between 100-150aa ______
10) Why is gene cluster in human #6 non-functional?__Critical enhancer deleted by __recombination event______
11) Why do humans #1, #2 and #3 all have red/green color vision? ______Because it doesn’t matter how many red and green genes one has in order to have red/green color vision______
12a-2pts) If the mouse genome had two arrangements of the pigment on two different chromosomes (see Figure 2), then how would this change your answer in questions 6 and 7?___One couldn’t tell which was the ancestral gene.______
12b-2pts) How could these two clusters (Figure 2) developed in the Mouse consistent with how the single cluster (Figure 1) developed in Humans? ______
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