“UTERINETONIC ACTIVITY OF AQUEOUS EXTRACT OF

Cocos nucifera Linn.INFLORESCENCE.”

SYNOPSIS FOR

M.PHARM DISSERTATION

SUBMITTED TO

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES,

BANGALORE, KARNATAKA.

BY

LAD SHRIRAM ASHOK.

DEPARTMENT OF PHARMACOLOGY,

NARGUNDCOLLEGE OF PHARMACY,

BANGALORE-560085.

(2008-2010)

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES

BANGALORE,KARNATAKA,

ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR PG DISSERTATION

1. / Name of the candidate and address(in block letters) / SHRIRAM ASHOK LAD.
NARGUNDCOLLEGE OF PHARMACY,
DATTATREY NAGAR, II MAIN,
BSK III STAGE,
BANGALORE-560085
KARNATAKA.
2. /

Name of the institution

/ NARGUNDCOLLEGE OF PHARMACY,
DATTATREY NAGAR, II MAIN,
BSK III STAGE,
BANGALORE-560085

KARNATAKA.

3. /

Course of study and subject

/

MASTER OF PHARMACY IN

PHARMACOLOGY

4. /

Date of the admission

/ 25th JUNE 2008
5.
6.0 /

Title of the topic:

“UTERINETONIC ACTIVITY OF AQUEOUS EXTRACT
OF Cocos nucifera Linn.INFLORESCENCE.”
BRIEF RESUME OF THE INTENDED WORK:
6.1 NEED FOR THE STUDY:
Woman of reproductive age (i.e. puberty to menopause) are most commonly affected by Endocrine diseases including diseases of uterus and ovaries. Disturbed menstruation, amnorrhea, variety of pain inand Polycystic ovarian syndrome (PCOS) are some of them.PCOS affects 5% of all women of reproductive age and is leading cause of infertility.
In PCOS ovaries are usually involved bilateral and are at least twice the size ofnormal ovary due to unbalanced release ofreproductive hormones.1To treat these problems mostly women prefer to use uterine tonics.Where uterine tonics are best known for their nourishing and toning function of uterus.
From ancient time mankind believes on herbal therapies. Now a day’sinterest is growing in herbal therapies due to their wide range of safety. The purpose of present study is to conduct experiment for usefulness of aqueous extract of Cocos nucifera Linn. Inflorescence as uterine tonic and to treat PCOS.
7.0
8.0 / 6.2REVIEW OF LITERATURE:
Family Name: Arecaceae
Botanical Name: Cocos nucifera Linn.
Vernacular Name: Sans- Narikelah ,Hind - Nariyal , Eng – Coconut
The coconut palm is grown throughout the tropical world and its every part is useful for man.
  1. Alanis AD, et al. has studied Anti bacterial properties of some plants (including Cocos
nucifera ) used in Mexican traditional medicine for the treatment of gastrointestinal
disorders.2
  1. Mendoca Filo Ricardo R,et al. has studied Leishmanicidal activity of polyphenolic rich extract from husk fiber of Cocos nuciferaLinn. (Palmae).3
  2. Alviano Daniela S, et al. has studied Antinociceptive and free radical scavenging activities of Cocos nucifera Linn. (Palmae) husk fiber aqueous extract.4
  3. Baheti Akshay M, et al. has studied chronic diuretic effect of the Bahirdhum Padhati Mashi(BPM) of unripe Cocos nucifera husk in normal rats.5
  4. Srivastava Pallavi, et al. has studied Burn wound healing property of Cocos nucifera.6
  5. Nneli RO,et al. has studied Antiulcerogenic effect of coconut (Cocos nucifera) extract in rats.7
  6. Corderio MS, et al. has studied the use of coconut water solution as a holding medium for in vitro embryo production.8
  7. MitraSK,et al. has studied Uterine Tonic activity of U-3107(Eve Care), a herbal preparation in rats.9
  8. Shrinivas K, et al. has studied Antibacterial activity of Cocos nucifera Linn.10
  9. Bipat Robert ,et al. has studied Effect of plants popularly used against hypertention on Norepinephrrine stimulated guinea pig atiria.11
6.3 OBJECTIVE OF THE STUDY:
1. Evaluation of uterine tonic activity of aqueous extract ofCocos nucifera Linn.
Inflorescence.
2. Evaluation of aqueous extract ofCocos nucifera Linn. Inflorescence to treat PCOS.
MATERIAL AND METHODS:
7.1SOURCE OF DATA :
Data is generated from the laboratory based animal studies and experimental data from library is also utilized.
7.2METHOD OF COLLECTION OF DATA:
The data collected will be based on animal experimentation as per the parameters studied under each animal model. The sampling procedure and collection of data will be as follows.
METHODOLOGY :
1) SCREENING OF UTERINETONIC ACTIVITY :
I.UTERINE WEIGHT9,12:
Animals:
Immature female wistar rats. Rats will be ovariectomised.
Four groups will be made.Each group will contain 8 rats.
Following groups will be made:
Group 01 (n = 8) : Vehicle
Group 02 (n = 8) : Standard
Group 03 (n = 8) : Test(Dose 1)
Group 04 (n = 8) : Test (Dose 2)
II. VAGINAL CORNIFICATION12:
Animals:
Immature female wistar rats. Rats will be ovariectomised.
Five groups will be made.Each group will contain 8 rats.
Following groups will be made:
Group 01 (n = 8) : Control
Group 02 (n = 8): Test (Dose 1)
Group 03 (n = 8): Test (Dose 2)
Group04 (n = 8): Standard (Dose 1)
Group 05 (n = 8): Standard (Dose 2)
2)EVALUATON OF POLYCYSTIC OVARIAN SYNDROME (PCOS):
Animals:
Female wistar rats.
Four groups will be made. Each group will contain 6 rats.
Following groups will be made:
Group 01 (n = 6): Normal
Group 02 (n = 6): Induction of multiple cyst
Group 03 (n = 6): Pretreatment with drug (Dose 1) + Induction of multiple cyst
Group 04 (n = 6): Pretreatment with drug (Dose 2) + Induction of multiple cyst
STASTICAL ANALYSIS:
The data will be analyzed using one-way analysis of variance (ANOVA).
7.3 Does the study require any investigation to be conducted on patients
Or other humans or animals? If so please describe briefly
Yes, The above study requires investigation on animals. The effect of drug will be studied
on various parameters using rat and mice as animal model.
7.4 Has ethical clearance been obtained from your institution in case of 7.3?
Yes, applied for “Institutional Animal Ethics Committee” of Nargund College of Pharmacy.
REFERENCES:
  1. Harsh Mohan. The Female Genital Tract. Textbook of Pathology. Fourth edition. New Delhi: Jaypee Brother (P) LTD; 2003.p. 727.
  2. Alanis AD, CalzadaF,Cebalos GM.Antibacterial properties of some plants used in Mexican traditional medicine for treatment of gastrointestinal disorders. Journal of Ethanopharmacology.2005; 100(1-2):153-157.
  3. Mendoca Filo Ricardo R, Rodrigues Igor A, Alviano Daniela S, Santos Andre LS, Soares Rsangela MA, Alviano Celuta S, Lopes Angela HCS, Socorros Rosa Maria Do, Leishmanicidal activity of polyphenolic rich extract from husk fiber of Cocos nucifera Linn. (Palmae).Research in Microbilogy.2004; 155(3):136-143.
4. Alviano Daniela S, Rodrigues Karen F,Matheus Maria Eline, FernandesPatricia D,
Antoniolli AngeloR, Celuta S Alviano. Antinociceptive and free radical scavenging
activities ofCocos nucifera Linn. (Palmae) husk fiber aqueous extract.Journal of
Ethanopharmacology.2004; 92(2-3):269-273.
5. Baheti Akshay M , Khandelwal KR, Ingale Suvarna P, Baheti Shikha A. Chronic
diuretic effect of the Bahirdhum Padhati Mashi(BPM) of unripe Cocos nucifera husk in
normal rats. Pharmacognosy magazine.2008; 4(15):215-217.
  1. Srivastava Pallavi, Durgaprasad S. Burn wound healing property of Cocos nucifera: An
Appraisal.Indian Journal of Pharmacology.2008; 40(4):144-146.
  1. Nneli RO, Woyike OA. Antiucerogenic effect of Coconut (Cocos nucifera) extract in rats.Phytotherapy research. 2008; 22(7):970-972.
  2. Corderio MS, Silva EHS, Miranda MS, Biondi FC, Santos SSD, Ohashi OM.The use of coconut water solution as a holding medium for in vitro embryo production.Anim Reprod. 2006; 3(3):376-379.
  3. Mitra SK, Gopumadnavan S, Venkataranganna MV, Sarma DNK, AnturlikarSD. Uterine Tonic activity of U-3107(Eve Care), a herbal preparation in rats.Indian Journal of
Pharmacology.1999; 31:200-203.
  1. Shrinivas K, Vijaysrinivas S, Kiran HR, Prasad PM, Rao MEB.Antibacterial activity of Cocos nucifera Linn. Indian Journal of Pharmaceutical sciences.2003; 65(4): 417-418.
  2. Bipat Robert , Toesle Jery R, Joemmanbaks Rouafsa F, Gummels Jill M, Klaveruide Julio,
Jhanjan Nawin, Sanilorie, Kiran Ramjiawan, Brussel Andy Van, Soekhoe Rubbania C,
Mans Dennis RA.Effect of plants popularly used against hypertention on Norepinephrrine
Stimulated guinea pig atiria.Pharmacognosy Magazine.2006; 4(13):12-19.
  1. Vogel H Gerhard, Vogel Wolfgang H , Schlkens Bernward A, Sandow Jurgen, Muller Gunter,Vogel Wolfgang F. Drug Discovery and Evaluation, Pharmacological Assay.[CD-ROM] Second ed.Verlag Berlin Heidelberg: Springer; 2002.p.1154, 1159-1160.

9 / SIGNATURE OF THE CANDIDATE
10 / REMARKS OF THE GUIDE / RECOMMENDED
11 / NAME AND DESIGNATION
11.1:- GUIDE / H J. HRISHIKESHAVAN PhD
PROFESSOR AND HEAD
DEPT. OF PHARMACOLOGY,
NARGUNDCOLLEGE OF PHARMACY,
Bangalore-560085.
11.2:- SIGNATURE
11.3:- CO-GUIDE / __
11.4:- SIGNATURE
11.5:- HEAD OF THE DEPT. / H J. HRISHIKESHAVAN PhD
PROFESSOR AND HEAD
DEPT. OF PHARMACOLOGY,
NARGUNDCOLLEGE OF PHARMACY,
Bangalore-560085.
11.6:- SIGNATURE
12 / REMARKS OF THE PRINCIPAL
12.1:- SIGNATURE

1