Department of Applied Sciences – Faculty of Engineering Technology: Instrumentation for Instrumental analysis, Analytical Chemistry, Organic Chemistry and General Chemistry Laboratories
No / Item / Quantity / Specifications1- / Preparative HPLC
modular, stackable, and comprised of a gradient pumping system, Autosampler, UV/visible spectrophotometric detector, fraction collector and software control and data processing package.
- Origin for this equipment (all parts, accessories, spare parts etc): USA, Europe, Japan
- Operating manuals and maintenance manual included.
- Training period required
Item / Quantity / specifications
Solvent delivery system:
High pressure gradient formation by the combination of two isocratic pump modules with appropriate connecting tubing and software control so that the units work together as one gradient solvent delivery system. / 1 / Each isocratic module shall have:
- Dual pistons in parallel
- Flow Range setpoints from 0 upto 100 mL/min, in 0.001 mL/min increments.
- Operating Pressure range from 2 to 40 MPa (20–400 bar, 290-5880 psi).
- Flow precision: <0.3 % RSD in range from 1 mL/min to 100 mL/min
- Pump Delay volume: 700 μL or less
- Tool kit
- Software controlled automated electromagnetic purge valve.
- Automated seal wash for extended seal lifetime. Safety features: Leak detection and safe leak handling (shuts system down if leak occurs), error detection and status display.
- Communications: Controller-area network (CAN) connection to other modules, RS232C, remote start/stop/prepare connection.
- Storage of electronic records of maintenance, usage and errors with notification when pre-set usage limits are reached to allow maintenance planning.
Autosampler / 1 / - Must be equipped with dual-injection-loop flow path for analytical and preparative scale injections without changing the loop.
- Must be installed with both 50 μL and 5000 μL (5mL) injection loops.
- Injection modes: Both Partial loop filling and Complete loop filling available on both loops.
- Injection range: 0.1 - 5000 μL in 0.1 μL increments.
- Area precision for Complete loop fill:
- Typically < 0.5% RSD (50 μL loop, 3x overfill).
- Sample viscosity range: 0.2 - 5 cp
- Carryover: < 0.1% with rinse factor > 3
- Ability to automatically rinse or flush the inside and outside of the injection needle with wash solvent for further reduction of carryover.
- Draw/eject rate of metering unit up to 50 ml/min (selectable in 1 μl/min steps)
- Must be upgradeable to thermostatted operation by simple addition of an optional Peltier temperature control module allowing temperature control of the samples from 4 to 40 °C.
Sample capacity, the following formats to be available:
- 2 well-plates (96 and 384) plus 10 additional 2-mL vials;
or - 108 x 2-mL vials in 2 × 54 vial plate plus 10 additional 2-mL vials;
or - 30 x 6-mL vials in 2 × 15 vial plate plus 10 additional 2-mL vials;
or - 54 Eppendorf tubes (0.5/1.5/2.0 mL) in 2 × 27 Eppendorf tube plate
Safety features:
- Leak detection and safe leak handling (shuts system down if leak occurs), error detection and status display.
Communications:
- Controller-area network (CAN) connection to other modules, RS232C, remote start/stop/prepare connection.
Storage of electronic records of maintenance, usage and errors with notification when pre-set usage limits are reached to allow maintenance planning.
Detector 1:
Multi-wavelength UV/visible detector / 1 / - Multi-wavelength UV/visible detector with minimum of 5 independent signals
- Wavelength range: 190 to 950 nm
- Signal bandwidth: variable from 2nm to 400nm
- Reference wavelengths and bandwidths can be set on all signals
- Light source: Both Deuterium and Tungsten lamps
- Detection type: photodiode array with resolution smaller than 1nm per diode
- Wavelength accuracy: ± 1 nm
- Verification of wavelength accuracy with built-in holmium oxide filter.
- Short term noise (ASTM) Single and Multi-Wavelength: < ± 0.7 x 10-5 AU at 254 and 750 nm
- Drift: < 0.9 x 10-3 AU/h at 254 nm
- Linear absorbance range: > 2 AU (5 %) at 265 nm
- Optical Slit width: Programmable with minimum 1nm, standard 4nm and maximum 16 nm resolution settings
- Supplied with preparative 3mm path length flow cell
- Supplied with preparative 0.3mm path length flow cell
- standard 10mm path length flow cell
- Safety features: Leak detection and safe leak handling (shuts system down if leak occurs), error detection and status display
- Communications: Controller-area network (CAN) connection to other modules, RS232C, remote start/stop/prepare connection
- Storage of electronic records of maintenance, usage and errors with notification when pre-set usage limits are reached to allow maintenance planning.
Detector 2: Refractive index / 1 / - Liquid contact material: stainless steel, PTFE and quartz glass.
- Range: 1.00 – approximately 1.8 RIU, calibrated
- Measurement range: +/- 600 × 106 RIU
- Temperature for optics: from ambient to 55 °C.
- Sample cell: 8 μL, maximum pressure: 5 bar(0.5 Mpa), maxiumum flow rate: 5mL/min.
- Valves: automatic purge and automatic solvent recycle.
- pH range: Approximate range 2 – 98 or better.
- Automatic zero before analysis
- Volume: Inlet port to sample cell 62 μL, inlet port to outer port 590 μL
- Time programmable parameters: polarity, peak width.
- Performance: short time noise < +/- 2.5 × 10-9 RIU, drift lower than 200 × 10-9 RIU/h
- Optical zeroing: through set screw
- Control and data evaluation: PC control with suitable software for control and data evaluation. Parameter entry, signal display, on-line help and diagnostic should be through control module. Optional card for method for method sequence and logbook storage and transfer.
- Max. Data rate: up to 37 Hz.
- Safety and maintenance: Extensive diagnostics, error detection and display, leak detection, safe leak handling, leak output signal and shutdown of pumping system.
- Communications: Controller-area network (CAN) connection to other modules, RS232C, remote start/stop/prepare connection.
- Storage of electronic records of maintenance, usage and errors with notification when pre-set usage limits are reached to allow maintenance planning.
Fraction Collector / 1 / - Delay calibration sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode, can be automatically positioned at end of fraction delivery needle to detect blue dye calibrant peak. Allows easy calibration of detector to fraction collector delay volume for accurate collection of fractions.
- Flow rate capability up to 100mL/min
- Allows pooling of fractions from multiple injections.
- Able to use well plates, Eppendorf tubes, and vials and test tubes of different heights and widths for fraction collection.
- Fraction containers:
96 deep-well plates up to 48 mm height;
Test tubes, minimum 48 mm up to maximum 100 mm height (up to 45 mL); Autosampler vials (2 mL and 6 mL)
Fraction collection full-size trays:
· 4 well plates
· 40 × 45 mL test tubes (30 mm OD, 100 mm height)
· 60 × 32 mL test tubes (25 mm OD, 100 mm height)
· 126 × 13 mL test tubes (16 mm OD, 100 mm height)
· 215 × 7.5 mL test tubes (12 mm OD, 100 mm height)
· 2 well plates + 10 x 2 mL vials (+ one possible additional half tray)
· 100 × 2 mL (+ one possible additional half tray)
· Tray for 40 collecting funnels
- Forced fume extraction to allow the use of autosampler outside fume cipboard.
- Fraction Collection Trigger modes: Time slices, Peak triggered from detector (threshold, up- / downslope), Timetable (combination of time intervals and peak)
- Must be upgradeable to thermostatted operation by simple addition of an optional Peltier temperature control module allowing temperature control of the fractions from 4 to 40 °C.
- Safety features: Leak detection and safe leak handling (shuts system down if leak occurs), error detection and status display
- Communications: Controller-area network (CAN) connection to other modules, RS232C, remote start/stop/prepare connection.
- Storage of electronic records of maintenance, usage and errors with notification when pre-set usage limits are reached to allow maintenance planning.
Control and Data Processing Software / 1 / - Software controls all functions of the LC modules
- Software shows status of instrument modules and parameters on a graphical user interface
- Allows methods to be stored containing the instrument parameters and data processing parameters
- Automated sequences can be setup to process multiple samples and to turn off the pump and detector at the end of the sequence.
- System offers standard qualitative and quantitative reports and customised reports
- Reports include fraction reports showing how the fractions were acquired and which containers they were stored into.
- Graphical setting of threshold, up-/downslope, upper threshold values on example chromatogram for ease of determining values.
Required additional items for the prep-HPLC:
Tool kit with screwdrivers and wrenches required for routine maintenance of system / 1
Analytical scale C-18 column / 1 / Analytical scale C-18 column
Prep Scale column / 1 / column C-18 column, 21.2 or 30 mm i.d.
Prep Scale C18 column, 50 mm i.d. / 1 / C-18 column, (50 mm i.d).
Vials / 1 / for use with auto-sampler, both 2mL and 6mL, and appropriate trays to hold them
Test tubes and trays for fraction collector / 1
ITEM / Quantity. / Specifications
2- / UV-Vis double beam Spectrophotometer / 1 / - 1nm Resolution or better, satisfying the standards of wavelength resolution demanded by the European Pharmacopoeia.
- Fully compliance with FDA 21 CFR Part 11.
- Has a built in USB port for direct connection with printer, data copy on a USB memory stick.
- Including software for data analysis Photometric analysis, spectrum mode, quantitation mode, kinetics mode, time scan, multi component quantitation mode.
- Including laser printer, Personal computer, LCD display screen with software for analysis and data processing.
- LCD display screen on the instrument showing spectrums for easy access for students
- Including a pair of UV Cell Quartz.
· Optical system: Double beam
· Wave length range: 190 – 1100 nm
· Spectral band width: 2 nm (190 – 1100 nm) or better
· Wavelength setting : 0.1 nm increments
· Wave length accuracy: ±0.3 nm
· Wave Length reproducibility: ≤ 0.2 nm
· Speed Scan: 3000 to 2 nm/min or better
· Photometric Mode: Transmittance, Absorption, Concentration
· Wavelength slew rate: about 6000 nm/min
· Photometric range: - 4.0 – 4.0 Abs, transmittance: 0% up to approximately 400 %
· Photometric accuracy: ± 0.002 A (0.0-0.5 A), ± 0.004 (0.5 – 1.0A), ± 0.006 Abs (2.0 Abs) and/or 0.3% T (0-100%T) or better.
· Stray light: ≤ 0.02% T (220 nm NaI, 340 nm NaNO2), less than 1.0% KCl (198 nm).
· Baseline stability: less than 0.0003Abs/hr (700 nm, 0Abs), after 2 hours of turning lamp on or use (or warm-up).
· Baseline flatness: about or within ± 0.0006 Abs (190-1100nm, one hour after light source turned on)
· Noise level: within 0.00005 Abs (700 nm) 30 min warm-up.
· Light source: Deuterium D2 and Tungsten halogen lamps, Built in light source and auto position adjustment
· Monochromator: Blazed holographic grating in Czerny-Tuner mounting
· Detector: Silicon photodiode.
· Connections and parallel port.
· Cell holder: 4 cell.
· Power supply: Switchable 120-230VAC, 50-60 Hz.
· PC (Brand name).
· Training included.
· Operation and service manuals are included.
· Certificate of conformity.
· Origin (for the instrument and all needed accessories: Europe, USA or Japan).
3- / Water jet vacuum pump / 1 / · Ultimate vacuum: 23 hPa (mbar) at 20°C and 7 hPa (mbar) at 1°C circulating water temperature
· Suction capacity: 4.500 L/h
· Supply: 230V/50Hz, 520W,single-phase
Origin (for the pump and all needed accessories: Europe, USA or Japan)
4- / Refrigerated bath circulator (Recirculating cooler) / 1 / · Capacity: 8-14 L
· Cooling capacity: 1200W at 10ºC
· Temperature range: -20 - + 30 °C
· Control accuracy: ±2ºC or better.
· Pump rate: 18-20 L/min
· Display: LED four digits display
· Material: - Bath: Stainless steel; Case: powder coated
· Safety: over current and leakage breaker.
· Approximate dimensions: overall (w× d×h, mm): 400 ×600 × 650
· Electrical supply: 230 V, 50-60 Hz
· Temperature controller: Digital PID controller
· Digitally set temperature
· Quiet operation
Origin (for the instrument and all needed accessories: Europe, USA or Japan).
5- / water-jet aspirator / 4 / · Portable dual-channel,
· Bath volume (L/cu ft) 9.5/0.3
· Max. vacuum capacity (Mpa/Hg): 0.0998/29.5
· Free air capacity: 18 L/minute (0.6 cfm × 2ea)
· Material: Path: polypropylene, Aspirator: Nickle coated brass X 2ea
· Dimensions: (bath (W × L, D) (mm/in) 298 × 210/11.7 × 8.3, 8.9
· Overall (W×L×H) (mm/in): 330× 265× 390/ 13.0 × 10.4× 15.4
· Inhale nozzle: internal diameter 9.5 mm (0.4)× 2ea
· Drain cook, over flow nozzle (mm/inch): Ø 17.0 /0.7
· Electrical requirements: 230 V, 50/60 Hz, 0.7 A
· Accessories: cooling coil, Aspirator
Origin (for the instrument and all needed accessories: Europe, USA or Japan, Korea).
6- / Electrical Heating mantel suitable for 2L Round Bottom flasks. / 2 / · Maximum temperature: 450 °C
· Indicator lamps for power and heater operation.
· A chemically resistant outer case with polypropylene or epoxy coating.
· Use with round bottom flasks 2L.
· Built in energy regulator.suppleid with clamps for 1.3 cm support tods.
· Certificate required.
· Nickel connectors.
· Thermally insulated case, mineral wool fiber insulation.
· Safety earth connection
· Dimensions (d x w x h), mm: 400 x 350 x 190
· 230V 50/ 60Hz, 500W, EU Plug
· Weight: Maximum 3 kg.
- Origin (for for the heating mantel and all needed accessories: Europe, USA or Japan).
7- / Digital Abee Refractometer / 4 / · Refractive index: 1.3000-1.7000 (± 0.0002); Brix Bx-TC: 0-95%, (± 0.1 %); Brix Bx: 0-95% (± 0.1 %), Temperature working range 0 -50 °C,
· - training included.
Origin: Europe, USA or Japan.
8- / Analytical Balance / 3 / § Fully automatic balance, 4 digits
§ Made of chemically resistant material
§ With glass shield side and top
§ Max capacity: 200 – 210 g
§ Readability: 0.0001g
§ Repeatability: ±0.0001g (0.1mg)
§ Pan size: Ø range 80-90 mm
§ Linearity: Linearity: ± 0.0002
§ Large easy to read high contrast LCD
§ Sealed keypad
§ Stainless Steel weighing pan
§ Internal, manual calibration
§ Automatic zero adjustment
§ Units of measurements: g, ct and oz.
§ Tare range by subtraction up to maximum capacity.
§ Level adjustment (bubble).
§ Operable temperature range ambient 5 °C to 40 °C.
§ Automatic calibration with external weight modes, Dust cover.
§ Stability time 10 seconds or better
§ Power supply 220-240 V
§ With English service and instruction manuals.
Origin: USA, EU or Japan
9- / Electronic precision balance / 4 / § Capacity: up to 410 g
§ Readability: 0.001g
§ Repeatability: ±0.001g
§ Linearity: ±0.002
§ Pan size: Ø approximately 120 mm
§ Large easy to read high contrast LCD
§ Sealed keypad
§ Stainless Steel weighing pan.
§ Protective in use cover.
§ Multiple weight modes.
§ Internal, manual calibration
§ Dust cover
§ Automatic calibration with external weight modes, Dust cover.
§ Power Supply: 230 Hz, 50-60 Hz
Origin: Europe, USA or Japan.
10- / Digital Avometer / 30 / · (digital).
· Origin: Europe, USA or Japan
11- / Analogue Melting point apparatus + thermometer / 4 / Melting point Apparatus, complete with thermometer and pack of 100 melting point tubes (open both ends).
· Number of samples: up to 3
· Temperature range: ambient to 300°C
· Heating rate: 1 to 10°C/min
· Net weight: should not exceed 2 kg.
· Electrical supply: 230 V, 50 Hz, 50 W.
· Easy to operate.
· Spare thermometer (2 for each apparatus)
· Origin: Europe, USA or Japan.
12- / Conductivity meters (bench top) / 4 / - Bench top with large screen
- Measures conductivity and TDS
- Both automatic and manual setting temperature
- Compensation with fixed coefficient, (2 % or better).
- Calibration with standards of conductivity and TDS.
- Temperature measuring range: 0-60 C.
- RS-232 output to connect to a printer or computer
- Temperature sensor connection Rt-2252.
- Conductivity ranges: 0-20, 200, 200 μS/cm; 0-10 mS/cm. precision: 1.5 % or better.
- TDS range: 0-1000 mg/L
- Operating manual + maintenance manual.
- Origin: USA, Europe, Japan
13 / Integrated GC-MS System / 1 / Fully operable of an integrated Gas Chromatograph/Mass Spectrometer (GC/MS) system of high performance with consistent ultra-trace detection limits is required. It has a simplified maintenance and easy to clean without tools. Also, it has the flexibility to choose the ideal level of sensitivity and dynamic range, and software for ease-of-use from data collection for evaluating and reporting. Also, sample handling system must be included. Educational with suitable set of experiments to be demonstrated.
- Original operating manual
- Full training programme on running the equipment and maintenance.
-- Manufacturer must be from either Jordan, USA, UK, Europe or Japan
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