Department:Clinical Laboratory Sciences
Course Number:CLS 212
Course Title:Medical Microbiology
Credit Hours:3 + 1 = 4
Course Description:
This is a general medical microbiology course intended for students outside the department of Clinical Laboratory Sciences.The structure of the course is based on presenting the fundamentals of microbiology to include structures, morphology and classification of bacteria, viruses, fungi and parasites. The students will be introduced to the pathogenesis of the various infectious agents. The course will also cover some topics related to community health, including the modes and sources of infections as well as prevention of these infections.
CLS 212: Lectures Outline
WeeksSubjects
1. General introduction
Historical Background & classification of microorganisms
2. Introduction to Viruses
Structure and morphology of viruses
Classification / Replication / Pathogenicity
3. Introduction to Fungi
Structure and morphology of fungi
Classification / Fungal diseases
4. Introduction to Parasites
Classification /General characteristics of protozoa
Medically important protozoa
General characteristics of helminths/Medically important
helminths
5. Introduction to Bacteria
Classification / Morphology
Bacterial Structures / Bacterial replication
6.Bacterial growth / Growth curve
Factors affecting growth
7. Antimicrobial agents
8. Microbial control / Principles
Physical and chemical methods
9. Microbial control – Chemical agents
Hospital acquired infections
10. Pathogenicity of infectious diseases
Normal microbial flora
11. Upper respiratory tract infections
12. Lower respiratory tract infections
13. Wound and skin infections
14. Sexually transmitted diseases
15. Food borne diseases, Water borne diseases
CLS 212: Laboratory Schedule
WeeksSubjects
1. Introduction to Microbiology laboratory
Techniques and safety rules
2. Introduction to light Microscopy
Types of light microscope
3. Examination of stained smear and wet mounts
4. Microscopic examination of eukaryotic microorganisms
5. Staining of bacterial cells and bacterial
structures ( simple and differential stains )
6. Bacterial culture media
Techniques of cultivation
7. Microbial flora of skin and oral cavity
Environmental sampling
8. Antimicrobial Agents - Methods of sensitivity testing
9. Microbial count : viable and total counts
10. Factors affecting microbial growth
11. Physical and chemical methods used in microbial
control
12. Revision
13. FINAL PRACTICAL EXAMINATION
Assessments:
First Mid Term Examination:15
Second Mid Term Examination:15
Laboratory Quizzes: 5
Mid Term Practical Examination: 5
Final Practical Examination:20
Final Theoretical Examination:40
References:
- Cano J. Raoul and Calome J.S., Microbiology, West Publishing Company, USA, Latest Edition
- Eugene W. Nester et al, Microbiology- A Human Perspective, Mc Graw Hill, USA,Latest Edition
- Tortora, G. J., B. R. Funke, and C. L. Case.,Microbiology, An Introduction, (7th edition) Benjamin Cummings, San Francisco, 2002
Department:Clinical Laboratory Sciences
Course Number:CLS 224
Course Title:Basic Anatomy & Physiology
Credit Hours:3 + 0 = 3
Course Description:
To acquaint the student with a comprehensive knowledge about the anatomy and physiology of the human body that help him to build his background information in the basic sciences that will enable him to understand the deviation from the normal in the different specialities that he/she may be directed to later on.
CLS 224: Lectures Outline
WeeksSubjects
- Introduction to Anatomy and Physiology:
-Body orientation, planes and sections, surface anatomy, body cavities and terminology, organ systems: an overview
-Body fluids and its distribution, mechanism of transport across the cell membrane, structure of the cell membrane
2. Cell and Tissues:
-Structure and function of cell
-Cell division
-Body tissue: Epithelial tissue, Connective tissue, Muscle tissue, Nervous tissue
3. Skin and Body Membrane:
-Basic structure and function of skin
-Appendages
-Classification of body membranes
4. Skeletal system:
-Functions, bone markings and classification of bones, structures of bones
-Applied aspects
5. Nervous System:
-Division by the Nervous system
-Nervous tissue – Neuroglia, neurons classification and their microscopic structure physiology – Nerve impulses
-Central Nervous System: Brain- different parts, Meninges and spinal cord, CSF
-Peripheral Nervous System: Cranial and spinal nerves, Important Nerves
-Autonomic Nervous System- Introduction
-Applied aspects
6-7.Cardiovascular System:
-Heart structure, External features
Chambers, valves, pulmonary and systemic circulation
Major arteries and veins of the body, special
circulation
Physiology: Cardiac cycle and heart sounds
-Conduction system of the heart
-Blood vessels, microscopic anatomy
-Blood pressure, Pulse
-Applied aspects
8. Respiratory System:
-Nasal cavity, pharynx, larynx, trachea, primary bronchi, lungs
-Physiology – Mechanics of respiratory gases and their transport, regulation of respiration
-Applied aspects
9. Digestive System:
-Organs of alimentary canal, mouth, pharynx, oesophagus, stomach, small and large intestines
-Accessory digestive organs, pancreas, liver and gall bladder, salivary glands and teeth
-Physiology- functions of the digestive system
-Applied aspects
10. Urinary system:
-Kidneys- location and structure of nephrons and urine formation, control of blood composition
-Characteristics of urethras, urinary bladder and urethra
-Micturition
11. Reproductive System:
-Male reproductive system
-Testes- structure and functions, spermatogenesis and testosterone production
-Duct system: Epididymis, Vas deferens
-Accessory glands
-External genitalia
12. Nerve and Muscle Physiology:
-Structure of the nerve, generation of action potential (nerve impulse), conduction of nerve impulse, types of nerves
-Structure, types of muscles, mechanism of muscle conduction, types of muscle contraction and concept of muscle fatigue
13. Blood Physiology:
-Composition of the blood
-Function of the different blood cells and the plasma
-The process of haematopoesis
-The mechanism of haemostasis, clinical aspects (Anaemia, haemophilia, Thrombocytopenia)
-Human blood groups, Blood typing
14. Endocrine Physiology:
-Nature of the hormone
-Name and site of the major endocrine glands
-Function of each gland and mechanisms of regulation of its function
-Abnormalities that result from over or under function of each gland
-Clinical aspects (Diabetes Mellitus)
Assessments:
First Mid Term Examination:30
Second Mid Term Examination:30
Final Theoretical Examination:40
References:
Elaine N Marieb,Essentials of Human Anatomy and Physiology, 7th Edition, Benjamin Cummings
Department:Clinical Laboratory Sciences
Course Number:CLS 222
Course Title:Descriptive Histology
Credit Hours:2 + 1 = 3
Course Description:
The overall objective of this course is to prepare the students for the advanced courses in CLS and enables the student to identify microscopically the normal tissues and organs of the human body.
CLS 222: Lectures Outline
WeeksSubjects
1. Introduction
2-3. Epithelial tissue:
(definition, features, classification and function)
4. Connective tissue:
(definition, features, cells and fibers)
Connective tissue types:
(loose, dense, reticular, adipose and elastic)
5. Supportive connective tissue
-Cartilage (general features, functions and types: hyaline, elastic, white fibrocartilage)
-Bone (general features, functions, types: spongy, compact and development)
6. Muscular tissue (general features and types)
7. Nervous tissue
-Central nervous system (parts), types and structure of neuron
-Peripheral nervous system: Types and structure of nerves and ganglia
-Nerve endings, Synapse
-Neuroglia
8. Circulatory system
-Blood circulation
-Heart structure and function
-Arteries and veins (types, structure and comparison of both)
-Comparison between capillaries and sinisoids
-Lymph circulatory system: lymph vessels
9. Lymphatic organs
-Lymph nodes: structure and function
-Spleen, thymus, tonsils
10. Respiratory system
-Upper: nose, nasopharynx, larynx
-Lower: trachea, bronchus, bronchiole
-Structure of the lung
-Pleura
11-12. Digestive system
-Oral cavity: lip, tongue (papillae and its function)
-Digestive tube: general structure of esophagus, stomach, small and large intestine, appendix
13. Digestive glands:
Salivary gland, liver, gall bladder and pancreas
14. Urinary system
Parts, function and structure of kidney and urinary
passage (ureter, urinary bladder and urethra in males
and females)
CLS 222: Laboratory Schedule
WeeksSubjects
1. Cell structure: electron microscopic study of cellular
Components (organelle and inclusions)
2. Demonstration of types of epithelium,
3. Demonstration of glands
4. Revision for epithelial tissue
5. Demonstration of cartilage types
6. Demonstration of CT types
7. Demonstration of bone types
8. Demonstration of types of muscles
9. Demonstration of nervous tissue
10. Demonstration of aorta, medium-sized artery and vein
11. Demonstration of Lymph organs
12. Practice on body organs (liver, lung and kidney)
13. Revision
14. FINAL PRACTICAL EXAMINATION
Assessments:
First Mid Term Examination: 15
Second Mid Term Examination: 15
Student activities, sharing, Quiz 10
Final Practical Examination: 20
Final Theoretical Examination: 40
References:
- Zakaria Abdulhamid,Histology I and II, Al Shaab Printing Press,Cairo house.
- Ira R. T. and Charlies F.B.,Introduction to functional Histology, Harper& Row, Pub, NY,London.
Department:Clinical Laboratory Sciences
Course Number:CLS 223
Course Title:General Pathology
Credit Hours:2 + 0 = 2
Course Description:
Upon completion of this course, the students will have the appropriate knowledge about the causes of cellular stress and the general pathologic responses to cellular stress, the circulatory disturbance, the basic immune reactions, the main immunopathologic processes, Disorders of growth and neoplasia.
CLS 223: Lectures Outline
WeeksSubjects
1. Introduction
-Define pathology
-Define disease, study of disease
2. Etiology
-Predisposing and determining factors
-Pathological anatomy (lesion)
-Manifestation of the disease
-Pathological physiology
-Diagnosis
3. Causes of cell damage (stress)
Cellular response to stress
Degeneration
Necrosis and gangrene
4-5. Inflammation (definition, nomenclature and causes)
Pathogenesis, local vascular changes of acute inflammation
The inflammation exudates, inflammatory cells and chemical mediators and sequelae of acute inflammation
-special types of acute inflammation
-types of chronic inflammation
general effects of inflammation
6. Infection
-factors influencing establishment of infection
-factors influencing the course of infection
-mechanism by which disease is produced: bacterial, viral and opportunistic infections
7. Healing
-wound healing
-factors influencing wound healing
-complications
-healing in special situations e.g. kidney, liver muscle, nervous tissue and bone
8-9. Circulatory Disturbances
-hyperemia
-congestion
-obstruction
causes: thrombosis, embolism
effect of vascular obstruction leading to oedema
-heamorrhage
10-11. Immunology
-specific and non-specific immunity
-immune response, antigen, antibodies, compliment, humoral cell mediated response
Diseases of the immune system
-hypersensitivity reactions
-auto-immune disease, tolerance
-immune deficiency diseases
12. Disturbances of growth
-hyperplasia
-hypertrophy
-atrophy
-dysplasia
-metaplasia
13-14. Neoplasia
-definition and classifications
-general features of benign and malignant tumors
-effects and complication of benign and malignant tumors
-tumor of epithelial, connective, muscular and nervous tissues
-carcinogenesis
-brief discussion on diagnosis of tumors and treatment
Assessments:
First Mid Term Examination:15
Second Mid Term Examination:15
Laboratory Quizzes: 5
Mid Term Practical Examination: 5
Final Practical Examination:20
Final Theoretical Examination:40
References:
Emanuel Rubin, Essential Pathology, Lippincott Williams&Wilkins, Phiadelphia & London.
Department:Clinical Laboratory Sciences
Course Number:CLS 231
Course Title:Clinical Analytical Chemistry
Credit Hours:2 + 1 = 3
Course Description:
The course involves a review of fundamental concepts of volumetric analysis as applied to biological fluids. The theoretical and practical aspects of different types of titri-metric analysis including acid-base, complexometric and precipitation titrations are studied.
CLS 231: Lectures Outline
WeeksSubjects
1. General introduction
-The analytical process
-Review of fundamental concepts
-Concentration of solutions
2. Expression of analytical results
-Principles of volumetric analysis
-Volumetric calculations
3,4,5. Acid – base Equilibrium
-The pH scale
-Buffers
-Buffers of biological and clinical significance
6,7. Complexometric titrations
-Complex and formation constants
-Chelates
-EDTA
-Indicators and detection of the end point
8,9. Precipitation titrations
-Vohlard method
-Mohr method
-The adsorption indicator method
10,11. Redox titrations
-Principles of Electrochemical cells
-the Nernst equation
-Iodometry
12. Gravimetric analysis
-Gravimetric factors
-Precipitation and co-precipitation
-Peptisation
13. Introduction to instrumental analysis
-Spectrophotometry
-Chromatography
CLS 231: Laboratory Schedule
WeeksSubjects
1. Basic laboratory equipment and procedures
safety rules
2. Standardization of sodium hydroxide and hydrochloric
acid solutions
3. Determination of bicarbonate in blood
4. Mercurimetric determination of blood and urine
chloride
5. Determination of blood glucose by a Redox
titration method
6. Complexometric determination of calcium in milk
7. Determination of the ionization constant of a weak acid
8. Determination of chloride by the Vohlard method
9,10. Gravimetric determination of chloride
11. Basic principles of visible spectrophotometry
12. Revision
13. FINAL LABORATORY EXAMINATION
Assessments:
First Mid Term Examination:15
Second Mid Term Examination:15
Laboratory Work and Reports:15
Final Practical Examination:15
Final Theoretical Examination:40
References:
Daniel C. Harris, Quantitative Chemical Analysis, W.H. Freeman & Company; 5th edition (August 1998).
Department:Clinical Laboratory Sciences
Course Number:CLS 232
Course Title:Clinical Biochemistry ( I )
Credit Hours:3 + 1 = 4
Course Description:
This course illustrates structures and functions of bimolecules which includes carbohydrates, proteins, lipids, nucleic acids, enzymes and hormones.
In the practical part of this course, students study the detection and quantitative determination of some of these biomolecules.
CLS 232: Lectures Outline
WeeksSubjects
1. Acids, bases, pH scale, buffer system
2. Chemical bonds
3. Amino acids:
structure, physical and chemical properties
4. Peptide bonds, proteins:
types, structure, functions
5. Protein purification
6. Nucleotides and nucleic acids
7. Enzymes
8,9. Carbohydrates
10,11. Fatty acids and lipids
12. Hormones
13. Vitamins
CLS 232: Laboratory Schedule
WeeksSubjects
1. Laboratory Safety
2. pH meter + colour tests of protein
3. Colour tests of amino acids
4. Estimation of protein:
Precipitation of proteins, plasma, serum and urine
protein Electrophoresis and TLC
5. Spectrophotometer + Enzyme kinetics
6. General colour tests of carbohydrates
7. Colour tests of pentoses, hexoses
8. General carbohydrate unknown, identification of
urinary sugars, paper chromatography
9. Determination of Glucose in blood plasma
10. Determination of acid value and iodine number of a fat
11. Determination of saponification value of fat
12. Determination of blood cholesterol
13. Revision
14. FINAL LABORATORY EXAMINATION
Assessments:
First Mid Term Examination:15
Second Mid Term Examination:15
Quizzes: 5
LaboratoryReports: 5
Final Practical Examination:20
Final Theoretical Examination:40
References:
- David L. Nelson and Michael M. Cox, Lehninger Principles of Biochemistry, W. H. Freeman; 4th edition (April 23, 2004).
- Pamela C. Champe and Richard A. Harvey, Lippincott’s illustrated reviews: Biochemistry, Lippincott Williams & Wilkins; 2 Sub edition, 1994.
Department:Clinical Laboratory Sciences
Course Number:CLS 241
Course Title:Haematology
Credit Hours:2 + 2 =4
Course Description:
This course teaches basic and diagnostic haematology with an emphasis on pathology. Normal hematopoiesis is covered in lecture, followed by a review of morphology of peripheral blood and bone marrow in lab and normal & abnormal haemoglobins.
Cell counting techniques; blood film staining and role of iron, vitamin B12 and folate. Anemias are covered through lectures, with morphology slides, and illustrative cases, which emphasizes to laboratory diagnosis.
Normal hemostasis, coagulation disorders, and thrombotic disorders are presented through lectures, case discussions and laboratory unknowns.
Morphology slides and clinical correlations are used to present myeloproliferative - lymphoproliferative disorders and acute leukemias.
An individually assigned case study at the end of the course emphasizes proper evaluation of laboratory data, microscopic analysis, and differential diagnosis of common hematologic disorders.
CLS 241: Lectures Outline
WeeksSubjects
1. Haemopoiesis: physiology and pathology
Red cells and platelets
2. Granulocytes, Monocytes reticuloendothelial system
3. Benign disorders of white cells: granulocytes
4. Red cell disorders
5. Haemostasis and Anticoagulation
6. Laboratory assessment
7. Iron: Physiology and deficiency Overload and sideroblastic anaemia
8. Megalohlastic anaemia:Vitamin B1, Folate deficiency
9. Haemolytic anaemias: Genetic defects of haemoglohn
10. Inherited defects of haemogolohin-sickle cell disease
11. Acute leukaemia
12. Chronic lymphocytic leukaemia
13. Myeloproliferative disorders
14. Clinical assessment
CLS 241: Laboratory Schedule
WeeksSubjects
1. Collection of blood from patients
2. Basic haematological techniques
3. Preparation and staining methods for peripheral blood
and bone-marrow films
4. Blood-cell morphology in health and disease
5. Blood-cell cytochemistry and supplementary techniques
6. Laboratory methods used in the investigation of the haemolytic anaemias
7. Investigation of the hereditary haemolytic anaemias
8. Investigation of the haemoglobinopathies
9. Laboratory methods used in the investigation of paroxysmal nocturnal haemoglobinuria (PNH)
10. Quantitative assay of coagulation factors
11. Investigation of platelet function
12. Laboratory control of anticoagulant and thrombolytic therapy
13. Investigation of megaloblastic and iron-deficiency anaemias
14. Miscellaneous tests
Assessments:
Mid Term Examination: 20
Mid Term Practical Examination:20
Assignment: 10
Final Practical Examination:20
Final Theoretical Examination:30
References:
- Atu Mehta & Victor Hoffbrand, Haematology at a Glance, ISBN 10-4051-2666-3
- Sir John V. Dace & SM Lewwis, Practical Hematology,ISBN: 0 443 01981 9
- Shirish M Kawthalkar, Essentials of Haematology, ISBN: 81-8061-633-9
- Hoffbrand AV, Pettit JE, PAH Moss, Essentials of Haematology, 4th ed. 2001, Blackwell Scientific Publications, ISBN 0632051531
Department:Clinical Laboratory Sciences
Course Number:CLS 311
Course Title: Basic Microbiology
Credit Hours:3 + 1 = 4
Course Description:
This course provides the students with basic theoretical and practical aspects of various groups of microorganisms to include bacteriology, virology, mycology, and parasitology as well as basic concepts of immunology and epidemiology. It also introduces the basic concepts of disinfectants, antiseptics, preservatives, Ames test, methods of sterilization, aseptic techniques and general microbial control.
CLS 311: Lectures Outline
WeeksSubjects
1. General introduction to Microbiology
Historical background and Classification of
Microorganisms
2. Introduction to Viruses:
Classification, morphology and structure, Replication and
Pathogenicity
3. Introduction to Fungi:
Classification, morphology and structure, Replication and
Pathogenicity
4. Introduction to Parasites:
Classification, General Characteristics of parasites and
Medically important Parasites
5. Introduction to Bacteria:
Classification, Morphology and Structures
6.Bacterial Structures
7. Microbial Growth requirements:
Nutritional requirements, Physical requirements,
Types of bacteria according to energy production
8. Bacterial Growth:
Growth curve, Constant and synchronous growth
9. Bacterial Metabolism: Catabolic Pathways and regulation
10. Bacterial Genetics:
Mutation and mutagenic agents ,&Ames test
Gene transfer ,PCR ,& genetic engineering
11. Bacterial Genetics continued ….
12. Microbial Control:
Principles, Disinfectants, antiseptics, andpreservatives
Physical and chemical methods of sterilization Aseptic
techniques
13. Microbial control continued…
14. Pathogenicity of Infectious Diseases
15. Normal Microbial flora
CLS 311: Laboratory Schedule
WeeksSubjects
1. Introduction to Microbiology laboratory techniques and
safety rules