Curriculum Vitae
General information:
Name: Golnaz
Surname: Bahramali
Date of birth: 17, Sep, 1980
Nationality: Iranian
Gender: Female
Address: Hepatitis and AIDS Dept., Pasteur Institute of Iran. Pasteur Ave, Tehran,
1316943551, Iran.
Tel: 09121165010
Fax: 00982166969291
E-mail: /
Education:
2010-2016: Ph.D. in Bioinformatics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
PhD thesis: Analysis of Chameleon Sequences and Presenting their role in some of the neurodegenerative diseases, using protein- protein interaction databases.(Supervisors: Professor Bahram Goliaei and Dr Zarrin Minuchehr)
2004 -2007: M.Sc. in Genetic, Modares University, Tehran, Iran
Master thesis: Detection of HBV genotype, subtype, BCP/Precore/Core and YMDD motif mutation profile among Iranian HBV infected patients
(Supervisor: Professor Majid Sadeghizadeh; Advisor: Dr. Fereidoun Mahboudi)
1999-2003: B.Sc. in Microbiology, Islamic Azad University of Tehran, Tehran, Iran.
Research Projects:
2006: A study on applicability of exploiting suicide gene therapy for selective killing the HTLV-1 infected cells.
2007-2008: Prevalence and genotype of hepatitis G virus in Human Immunodeficiency Virus (HIV) infected patients.
2009-2012: Immunogenicity and production of poly/mono clonal antibodies against ARFP/F (core+1) protein of HCV by utilization of epitopic-synthetic peptides & core+1 recombinant protein-Evolution of new human compatible adjuvants and different myeloma cells.
2009-2011: Impact of Occult Hepatitis B infection on Interferon therapy in HCV infected patients referring to Tehran Hepatitis Center in 1388-89.
2010-2012: Comparative study on mutations associated with Lamivudine resistance in HBV and HBV/HIV co-infected patient.
2012- 2014: Investigating the evolution of HCV NS5A region in relation to Peg IFN+RBV therapy in Iran.
2010-2013: Design and Evaluation of Immunological characteristic of mzNL4-3-NS3 in BALB/c mice.
2014: Design and Evaluation of Immunological characteristic of P protein from baculovirus expression system.
2015: Identification of host's protein targeted by HIV-1 Vpr for proteasomal degradation.
2016: Prevalence evaluation of hepatitis A virus (HAV) antibody among Iranian prisoners.
مصوب شورای عتف E طرح ملی تولید واکسن هپاتیت همکار -
مصوب وزارت بهداشتA- همکار طرح ملی تولید واکسن هپاتیت
Techniques:
Highly skilled in:
· Bioinformatics software & tools
· Protein structures
· Molecular Visualization Software: UCSF Chimera, RasMol, VMD, ...
· Systemsbiology, Protein- Protein interaction network, Gene and diseases network
· Programming: C++, C Sharp
· Phylogenetic analysis
· Sequence analysis: Bioedit, Clustal X, Mega 4
· Primer design
· Nucleic acids (DNA and RNA) extraction methods.
· PCR based methods; conventional PCR, RT-PCR and Real-Time
· PCR Molecular Genotyping by different methods like PCR-RFLP.
· Microbiological Techniques: Bacterial culture, Transformation.
· Immunological and Biochemical Techniques: Spectrophotometer, Gel electrophoresis, SDS- PAGE, Ion –Exchange Chromography, Western Blotting, Protein Purification in E. coli.
· ELISA
· Statistics: SPSS
Teaching:
2017: Primer Design workshop. Pasteur institute of Iran.
2017: "Bioenergetic " to M.Sc. student. Science and research branch of Islamic Azad university. Tehran-Iran.
2016: Design of candidate vaccines by immune informatics. winter school. Pasteur institute of Iran.
2016: Application of bioinformatics in protein analysis. winter school. Pasteur institute of Iran.
2015: Bioinformatics, application in DNA analysis. winter school. Pasteur institute of Iran.
2015: Bioinformatics, application in protein analysis. winter school. Pasteur institute of Iran.
2014: prediction of three-dimensional structure of protein, Pasteur institute of Iran, winter school.
2013: Genetics
Published papers:
1- Golnaz Bahramali, Bahram Goliaei, Zarrin minuchehr, Sayed‑Amir Marashi.A network biology approach to understanding the importance of chameleon proteins in human physiology and pathology, Amino Acids,2016.
2- Golnaz Bahramali, Bahram Goliaei, Zarrin minuchehr, Ali salari, Chameleon sequences in neurodegenerative diseases, BBRC, 2016, 472 (2016) 209e216.
3- Zabihollahi1 R, Pooshang Bagheri K, Keshavarz Z, Motevalli F,Bahramali G, Siadat SD, Momen SB, Shahbazzadeh D, Aghasadeghi MR.Venom Components of Iranian Scorpion Hemiscorpius lepturus Inhibit the Growth and Replication of Human Immunodeficiency Virus 1 (HIV-1). Iran Biomed J. 2016 Nov;20(5):259-65.
4- Mostafavi SS, Ebrahimi A, Sadat SM, Davari Tanha F, Aghasadeghi MR,BahramaliG, Abbasi Ranjbar P, Sadeghifard V, Javadi F.Impact of null genotypes of GSTT1 and GSTM1 with uterine leiomyoma risk in Iranian population. 2016, J Obstet Gynaecol Res. 42(4):434-9.
5- Rahimi P,Roohandeh A,Sohrabi A,Mostafavi E,Bahram AliG. Impact of Human Enterovirus 71 Genotypes in Meningoencephalitis in Iran. 2015, Jundishapur J Microbiol. 26; 8(12):e27113
6- Ardestani MS1,Fordoei AS,Abdoli A,Ahangari Cohan R,Bahramali G,Sadat SM,Siadat SD,Moloudian H,Nassiri Koopaei N,Bolhasani A,Rahimi P,Hekmat S,Davari M,Aghasadeghi MR. Nanosilver based anionic linear globular dendrimer with a special significant antiretroviral activity. 2015.J Mater Sci Mater Med. May;26(5):179
7- Zavvar M, Moshiri A, Motevali F, Pouriayevali MH and Bahramali G. Construction of pIRES2 vector encoding truncated NS3 of HCV and IL-18 for DNA vaccine studies.2015, vaccine research.1.num3.24-28
8- Ramezani A, Aghasadeghi MR, Ahmadi F, Razeghi E, Eslamifar A, Banifazl M, Sofian M,BahramaliG, Hekmat S, Aghakhani A.Isolated anti-hbc and occult HBV infection in dialysis patients. 2014 Nephrourol. 7(1): e22674.
9- Aghasadeghi MR, Delbaz SA, Sadat SM, Siadat SD, Ardestani MS, Rahimi P, Bolhassani A, Roudsari RV,Bahramali G, Motevalli F, Davari M, Vakily H, Salmani AS, Nobari MB. Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes.2014. Avicenna J Med Biotechnol.6 (4):238-45.
10- Rahimi P, Naser HM, Siadat SD, Sohrabi A, Mostafavi E, Motamedirad M, Bahramali G, Sadat SM, Ardestani MS. Genotyping of human parechoviruses in Iranian young children with aseptic meningitis and sepsis-like illness. J Neurovirol.2013; 19(6):595-600.
11- Zeinab Vakili, Seyed Moayed Alavian, Rouhollah Vahabpour, Golnaz Bahramali, Ehsan Mostafavi, Mohammad Reza Aghasadeghi and Safieh Amini.Prevalence of occult hepatitis B virus in plasma and peripheral blood mononuclear cell compartments of chronic hepatitis C infected patients in Tehran-Iran. Hepat Mon. 2013; 13(5): e10134
12- Mohammad Reza Aghasadeghi, Minoo Mohraz , Golnaz Bahramali , Arezoo Aghakhani ,Mohammad Banifazl , Maryam Foroughi , Farrokhlagha Ahmadi , Ali Eslamifar , Seyed Mehdi Sadat 1, Amitis Ramezani. Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis. Hepat Mon. 2013 3(5): e7481.
13- Naddaf SR, Ghazinezhad B, Bahramali G, Cutler SJ. Phylogenetic analysis of the spirochete Borrelia microti, a potential agent of relapsing fever in Iran. J Clin Microbiol. 2012; 50(9):2873-6.
14- Safieh Amini, Seyed Moayed Alavian, Ehsan Mostafavi, Rouhollah Vahabpuor, Golnaz Bahramali, Mohammad Reza Aghasadeghi & Arash Arashkia. Presence of plus-strand HCV RNA in serum and PBMC as an indicator for relapse and resistance to IFN therapy in patients infected by HCV. Future Virol. 2012 .7(3):323-330
15- Aghasadeghi MR, Bahramali G, Sadat SM, Farahani far A, Mohraz M, Siadat SD, Mostafavi E, Memarnejadian A, Shafiee Ardestani M1, Vahabpour R, Azizi Saraji AR, Delbaz SA Detection of Hepatitis B virus variants in HBV monoinfected and HBV/HIV coinfected Iranian patients under Lamivudine treatment. Curr HIV Res. 2011, 9:263-269.
16- Naddaf SR, Razavi MR, Bahramali G.Molecular variation and distribution of Anopheles fluviatilis (Diptera: Culicidae) complex in Iran.Korean J Parasitol. 2010 Sep;
48(3):231-6. Epub 2010 Sep 16.
17- Golnaz Bahramali, Majid Sadeghizadeh, Samad Amini-Bavil-Olyaee, Seyed- Moayed Alavian, Abbas Behzad-Behbahani, Ahmad Adeli, Mohammadreza Aghasadeghi, Safieh Amini.Clinical, virological and phylogenetic features of hepatitis B infection in Iranian patients. World J Gastroenterol. 2008 Sep 21; 14(35):5448-53.
18- Hekmat S, Mohraz M, Vahabpour R, Jam S, Bahramali G, Banifazl M, Aghakhani A, Eslamifar A, Mahboudi F, Edalat R and Ramezani A. Frequency and genotype of GB Virus C among Iranian patients infected with HIV. J Med Virol. 2008 Nov;80(11):1941-6.
19- Aghasadeghi M.R., Sadat S.M., Khabiri A.R., Amini S., Budkowska A, Bahramali G., Naddaf S.R. and Roohvand F. Evaluation of a native preparation of HCV core protein (2-122) for potential applications in immunization, diagnosis and mAb production. Iranian journal of public health Infection, 13(S1), S 609-S 676. 2006, 35(1):1-10.
20-سلوا سادات مصطفوي ده رئيسي سيد مهدي سادات ، فاطمه داوري تنها ، محمدرضا آقاصادقي، گلناز بهرامعلي ، گلوتاتيون در استعداد ابتلا به میوم رحمی. مجله ، S نقش ژن پلي مورفيسم هاي شايع. مهدي صفرپور 3 139 . دانشكده. پزشكي
21-ليلا خجاره، مجيد صادقي زاده، گلناز بهرامعلي، سامان حسينخاني، مهرداد بهمنش.تشخيص موتاسيون مقاوم به دارو ناحيه YMDD ژن پلي مراز ويروس HBV دربيماران مبتلا به هپاتيت B از طريق روش. (Ligase Chain Reaction (LCRفصلنامه تازه هاي بيوتكنولوژي سلولي مولكولي ، سال اول، شماره .4
22- فاطمه متولی ، ارش معمارنژادیان، سید مهدي سادات، گلناز بهرامعلی ،فرزین روحوند. مقایسه اپی توپ های CD8 ویروس هپاتیت C به صورت تک اپی توپ مخلوط اپی توپ ها و پپتید پلی توپ. فصلنامه علوم پزشکی اسیب شناسی زیستی مدرس -زمستان 1390-شماره 4 - صفحات 61 تا71.
23-محمدرضا آقاصادقی، مینو محرز، آرزو آقاخانی، گلناز بهرامعلی، محمد بنی فضل ، مریم فروغی، فرخ لقا احمدي، علی اسلامی فر و آمیتیس رمضانی. شیوع عفونت ویروس هپاتیت D در بیماران همودیالیزي و HIV مثبت.فصلنامه بیماري هاي عفونی و گرمسیري وابسته به انجمن متخصصین بیماري هاي عفونی و گرمسیري- سال شانزدهم، شماره 54 - صفحات 41 ت46.
24- دکتر محمدرضا آقاصادقی، احمد فراهانی فر، گلناز بهرامعلی، فوزیه جوادي- هپاتیت حاد در دوران حاملگی.
مجله بهداشت جهان- شماره دوم و سوم. 1388-
25-احمد فراهانی فر، محمدرضا آقاصادقی، گلناز بهرامعلی، سید مهدي سادات، سید داور سیادت، مینو محرز، مریم فروغی، فوزیه جوادي، عباس بهزاد بهبهانی، فرزین روحوند، صفیه امینی، محمدعلی داور پناه، مهین جمشیدي ماکیانی محمدصادق خسروي، سید بهمن مومن- فراوانی ژنوتایپ. ساب تایپ هاي HBV و HIV/HBV همزمان به تنهایی- در بیماران مبتلا به عفونت. HBV مجله میکروبشناسی پزشکی. ایران شماره 1- بهار. 1389
26-فاطمه سالم، دکتر محمدرضا آقاصادقی، فوزیه جوادي، دکتر فرزین روحوند، مهسا جولایی، گلناز بهرامعلی،
احسان مصطفوي ، دکتر حسین غلامی، دکتر سهیلا حکمت استفاده از روش هاي مولکولی RLFP-PCR و توالی یابی مستقیم جهت تعیین ژنوتایپ هاي هپاتیت B در زندانیان معتاد به مواد مخدر تزریقی استان تهران -مجله دانشگاه علوم پزشکی ایران شماره 71 - اردیبهشت .1389
27- محمدرضا آقاصادقی، سید مهدي سادات، گلناز بهرامعلی، سهیلا حکمت، فاطمه متولی، شهاده علیزاده ، سمیه کدخدائیان،فرزین روحوند- ارزیابی چند سیستم ادجوانت جدید براي ایجاد پاسخ ایمنی همورال و سلولی در ایمنی زایی با پروتئین کپسید ویروس هپاتیت .Cمجله علمی و پژوهشی فیض – شماره 1- بهار .1389 -C
28- آمیتیس رمضانی، سهیلا حکمت، سارا جم، روح الله وهاب پور، گلناز بهرامعلی، آرزو آقاخانی علی اسلامی فر، محمد بنی فضل، مطیف گچکار- مینو محرز. (1387) فراو انی و ژنوتایپ ویروس هپاتیت در بیماران آلوده به ویروس نقص ایمنی انسانی. فصلنامه بیماریهاي عفونی و گرمسیري وابسته به انجمن متخصصین بیماریهاي عفونی و گرمسیري: شماره 41 ، ص7-12.
Congress presentations:
1- AcrB, the inner membrane component of Multidrug efflux system, of Escherichia coli has the highest number of chameleon sequences. Golnaz Bahramali, Bahram Goliaei, Zarrin Minuchehr and Bijan Bambai. International Conference on Intelligent Biology and Medicine, San Antonio, Texas, USA 2016.
2- Determination and Analysis of Chameleon protein sequences in the Protein Data Bank (PDB). Golnaz Bahramali, Bahram Goliaei, Zarrin Minuchehr and Majid Reza Ghatee International Conference on Intelligent Biology and Medicine, San Antonio, Texas, USA.2014.
3-Determination of hepatitis B virus reverse transcriptase mutations in nucleos(t)ide analogue naïve patients with chronic hepatitis B.F. Bakhshizadeh, S hekmat, Maryam K, S. Moayed alavian, Hossein Keyvani, fatemeh Motevali, Golnaz Bahramali. The Viral Hepatitis Congress 2013.
4- Two Nestd-PCR Methods for Human n and Optimization of Comparation Adenovirus Detection in HIV Positive Patients. Sadat SM Zavvar M,Pouriayevali MH , Javadi F,Aghasadeghi MR,Khosravi MS, Amini S, Bahramali G, Zabihollahi R1, Shafiee Ardestani M. 8th National congress of Biotechnology,Tehran, Iran, 2013.
5-M. R Aghasadeghi,A. farahani, Bahramali G., S.M. Sadat, A. Memarnejadian. S.D Siadat, M. Mohraz,M. Foroughi, F. Javadi,F. Roohvand,R. Vahabpoor,M.R Pouriayevali- Prevalence of HBV Lamivudine- Resistance Mutations in a cohort of Iranian HBV- infected patient with and without HIV co-infection. ESCMIDP1145- 2010.
6-HBV Genotypes and Subtypes in Iranian HBV infected patients with and without HIV infection. Ahmad Farahani Far, Mohammad Reza Aghasadeghi, Golnaz Bahramali, Seyed Mehdi Sadat, Seyed Davar Siadat, Foozieh Javadi, Farzin Roohvand, Safieh Amini, Sohila Hekmat, Arash Memarnejadian, Fatemeh Motevalli, Mahsa Jolaei,The
3rdeurasia congress of infectious diseases, 1-4 October 2009.
7-Comparative studies on mice immunization with HCV minimal-CTL epitopic peptides by mixed or polytope formulations. Fatemeh Motevalli, Mohammad Reza Aghasadeghi, Arash Memarnejadian, Seyed Mehdi Sadat, Golnaz Bahramali, Shahadeh Alizadeh, Farzin Roohvand.10th international congress of asthma and allergy. Shahid Behesti University of medical sience.
8-Studies on Immune-dominancy of HCV-CTL epitopes by peptide immunization in BALB/c Mice.Arash Memarnejadian, Seyed Mehdi Sadat, Mohammad Reza Aghasadeghi, Fatemeh Motevalli, Golnaz Bahramali, Farzin Roohvand. 10th international congress of asthma and allergy. Shahid Behesti University of medical sience.
9-Genotyping and phylogenenetic analysis of HBV based on S region in Iranian IVDU prisoners. S. Hekmat, M.R. Aghasadeghi, F. Salem1, F. Javadi, M. Jolaei1, Bahramali, Accepted for the Second International Conference on Infectious Disease
Dynamics, 2-4 December 2009.
10- Evaluation of humoral and cellular responses to combination of different human compatible adjuvants and HCV core protein in immunized mice. P2, Research on infectious diseases, M.R. Aghasadeghi, M Sadat, G.Bahramali, F.Motevalli, A.Budkowska and F.Roohvand: A Global Challenge, Institut Pasteur, Paris, France,
2008.
11 - Production of native recombinant HCV core protein for diagnostic and therapeutic (vaccine) applications. M.Sadat, M.R. Aghasadeghi, G.Bahramali, F.Motevali, A. Budkowska and F.Roohvand. P70, Research on infectious diseases: A Global Challenge, Institut Pasteur, Paris, France. 2008.
12- Frequency of mutations in the Core promoter and PreCore region in anti-HBe positive with viral therapy in Iranian HBV carriers. Bahramali G., Mahboudi F., Amini-Bavil-Olyaee S., Alavian S.M., and. 6th international symposium on Antimecrobial Agents and Resistance.Singapore,2007.
13-Detection of drug resistance mutations in the YMDD and FLLA regions of polymerase gene of HBV in Hepatitis B patients by ligase chain reaction.
Khojareh Leila, Bahramali Golnaz, Sadeghizadeh Majid, Behmanesh Mehrad, Husseinkhani Saman. 15th National 3rd International conference of Biology,August 2008.
14- Ligase Chain Reaction (Lcr) as a tool for detection of drug resistance – mutation in YMDD and FLLA motif. Khojareh Leila, Bahramali Golnaz, Sadeghizadeh Majid, Behmanesh Mehrad, Husseinkhani Saman. 2nd International student conference of biotechnology.
15- Frequency of mutations in the core promoter/Precore and YMDD motif in HBV among Iranian HBsAg carriers in different stages of the disease. Bahramali1 G., Mahboudi F., Amini-Bavil-Olyaee S., Alavian S.M., Behbahani B., Rabbani B., and Sadeghizadeh M., Fourth Iranian Congress of Virology, May 1st - 3rd. P64. 2007.