Annexure ‘CD-01’

L / T / S / SW/FW / P / TOTAL CREDIT UNITS
2 / 0 / 0 / 0 / 2 / 3

Course Title:Elements of Biochemistry

Course Code: to be decided later

Credit Units:

Course Objectives: This course covers the novel concepts of biomolecules, their pathway and basic biochemical techniques and their application.

Pre-requisites: Basic Chemistry

Course Contents/ Syllabus:

Weightage (%)
Module I Introduction / 15%
  • Introduction to Biochemistry
  • water as a biological solvent
  • weak acid and bases
  • pH
  • buffers: Handerson-Hasselbalch equation
  • physiological buffers
  • fitness of the aqueous environment for living organism

Module II Biomolecules / 25%
  • Introduction of bio-molecules
  • Carbohydrates
  • Proteins
  • classification based on solubility, shape, composition and functions
  • Amino acids, lipids and Nucleic acid
  • Nature of genetic material: Evidence that DNA is the genetic material, composition of DNA and RNA
  • generalized structure plan of nucleic acids

Module III Enzymology / 20%
  • History, general characteristics and classification of enzymes
  • Definition with examples of holoenzyme, apoenzyme, coenzymes
  • cofactors activators, inhibitors, active site, units of enzyme activity,
  • specific enzymes
  • Enzyme Kinetics: Factors affecting enzyme activity : enzyme concentration, substrate concentration, Michaelis–Mentenconstant, pH and temperature
  • Concept of Enzymes Immobilization and methods.

Module IV Metabolism and Concept on Bioenergetics / 20%
  • Metabolism and bioenergetics
  • Generation and utilization of ATP
  • Metabolic pathways and their regulation: glycolysis, TCA cycle,
  • Biological membranes.
  • Transport across membranes, oxidative phosphorylation

Module V Biochemical Techniques / 20%
  • Chromatography: General principles and applications of: Paper chromatography. Thin layer chromatography,
  • Spectroscopic Techniques Beer-Lambert’s law, light absorption and its transmittance, determination and application of extinction coefficient, application of visible and UV spectroscopic techniques,
  • Electrophoresis Basic principles of electrophoresis; polyacrylamide Gel (PAGE)

Student Learning Outcomes:

•Learn Basic concept of Biochemistry- and its applicability for the attachment/linkage of various molecules.

•Students will learn how the energy rich molecules can be formed and utilized for energy generation and storage

•They will also get expertise in specific characterization techniques useful for separation of different types of biomolecules and thus can used them for generation of fuels and energy

•To apply the knowledge of analog circuits to generate energy/ power.

Pedagogy for Course Delivery:

The class will be taught using theory/Practical covering from standard text books. In addition the course instructor will update the knowledge of latest state of art of the respective subjects from various media. The instructor will cover the ways to analyse experimental data and think innovatively and liberally

(a)Lectures Plan/ Session Plan

(b)Lab/ Practicals

List of Experiments

•Experiment 1: Preparation of Buffer Solution of different Molarities and pH

•Experiment 2: Quantitative analysis of carbohydrates i.e sugars. (Molischs test, iodine test, Fehlings test, Benedicts test)

•Experiment 3: Gel electrophoresis for separation of bimolecular

•Experiment 4: To separate mixture by paper chromatography

•Experiment 5: Colorimetric Test for enzyme activity.

Assessment / Examination Scheme:

Theory L/T(%) / Practical(%)
67 / 33

Theory Assessment (L&T):

Continuous Assessment/Internal Assessment / End Term
Examination
Component
(Drop down) / Class Test
(CT) / Home Assignment
(HA) / Viva
(V) / Attendance
(A)
Weightage
(%) / 15 / 5 / 5 / 5 / 70

Lab/Practical/Studio Assessment:

Continuous Internal Assessment--- / End Term
Examination
Component
(Drop down) / Practical
(PR) / Viva
(V) / Lab Record
(LR) / Attendance
(A) / (PR) / (V)
Weightage
(%) / 10 / 5 / 10 / 5 / 35 / 35

Text & References:

  • Lehninger’s Principles of Biochemistry by David L. Nelson and Michael M. Cox, Macmillan Worth publisher.
  • Lubert Stryer, Biochemistry, 4th Edition, WH. Freeman and co., 2000.