(copy and paste into your word processor and submit on your letterhead)
Interstate Movement Notification Letter
Permits Branch
Biotechnology Permits
Biotechnology Regulatory Services
4700 River Road, Unit 147
Riverdale, MD 20737-1228
FAX 301-734-8910
1. Reference Number: (Leave blank)
2. Applicant Reference Number:
3. Applicant/Responsible party:
4. Duration of Introduction:
Interstate Movement: (dates) (up to 1 year)
5. Recipient: Rice (Oryza sativa)
6. Regulated Article: Transposon transformed line
a) designation of transformed line: RGT_____
category: Other
phenotype: Transposon inserted
construct: pDsGTR (Dissociation Element Gene Trap Rice)
genotype: Niponbare
Marker gene
promoter: Zea maize Ubiquitin 1
gene: DsRed (red fluorescent protein), coral Discosoma sp.
terminator: nopaline synthase (nos) 3' from
Agrobacterium tumefaciens T-DNA
Reporter gene
Gene: glucuronidase (uidA) from E. coli
terminator: nopaline synthase (nos) 3' from
Agrobacterium tumefaciens T-DNA
Transposon: Zea maize Dissociation Element
b) designation of transformed line: RDs_____
category: Other
phenotype: Transposon Inserted
construct: pDsR (Dissociation Element Rice)
genotype: Niponbare
Marker gene
Promoter: Maize Ubiquitin 1
gene: DsRed (red fluorescent protein), from coral Discosoma sp
terminator: nopaline synthase (nos) 3' from
Agrobacterium tumefaciens T-DNA
Transposon: Zea maize Dissociation Element
c) designation of transformant line: RdSpm____
category: Other
phenotype: Transposon inserted
construct: pdSpmR(defective Suppressor-mutator transposable element)
genotype: Niponbare
promoter: Zea maize Ubiquitin 1
gene: DsRed (red fluorescent protein), from coral Discosoma sp.
terminator: nos 3' from A. tumefaciens T-DNA
Transposon: Zea maize dSpm (defective Suppressor-mutator transposable element)
d) designation of transformant line: RGdSpm____
category: Other
phenotype: Transposon Inserted
construct: pdSpmG (defective Suppressor-mutator transposable element
GFP)
genotype: Niponbare
promoter: 35S from CaMV (Cauliflower Mosaic Virus)
gene: GFP (Green Fluorescent Protein), jellyfish Aequorea Victoria
terminator: nos 3' from A. tumefaciens T-DNA
Transposon: Zea maize dSpm (defective Suppressor-mutator transposable element)
7. Mode of Transformation: disarmed A. tumefaciens,
8. Introduction:
Interstate Movement:
ORIGIN: Genetic Resources Conservation Program
University of California
One Shields Avenue
DavisCA95616USA
County of Yolo
DESTINATION:
9. Certification: I certify that the regulated article will be
introduced in accordance with the eligibility criteria and the
performance standards set forth in 7 CFR 340.3. The above
information is true to the best of our knowledge.
If there are any changes, we will contact APHIS.
Signature ______Date ______
Name Typed ______