Table S1. Proximate analysis and anti-nutritional compounds in the transgenic bean line Embrapa 5.1 and its counterpart (cv. Olathe). Grains were harvested from plants grown under field conditions in three locations in 2008.
Component / Londrina / Santo Antônio de Goiás / Sete Lagoas / Olathe DB / Databank(reference range)
Olathe / Embrapa 5.1 / Olathe / Embrapa 5.1 / Olathe / Embrapa 5.1
Moisture / 10.00±0.54 / 9.08±0.93 / 9.54±0.83 / 8.61±0.54 / 9.38±0.77 / 8.00±0.82 / 9.23-13.85 / 9.23-14.78
Ash / 4.40±0.95 / 3.71±0.50 / 4.53±0.21 / 4.31±0.47 / 3.82±0.84a / 4.5±0.42 / 3.34-5.12 / 3.34-5.53
Protein / 18.95±0.97 / 18.91±0.58 / 19.52±0.89 / 21.15±0.96 / 19.86±0.26 / 20.13±0.90 / 14.2-27.2 / 13.46-27.2
Phytic acid / 11.56±0.64 / 11.65±0.65 / 8.72±0.43 / 9.19±0.36 / 11.27±047 / 12.81±0.39 / 3.47-12.28 / 3.01-13.45
Trypsin inhibition / 220.1±10.6 / 211.1±10.7 / 162.0±5.4 / 162.6±10.9 / 184.6±10.9 / 182.6±8.1 / 101.9–295.2 / 101.9-392.7
Values are mean ± SEM. n= 3.
Moisture, Ash and Protein are in g/100g; Phytic acid is in mg/g and Trypsin Inhibition is in TIU/100g.
a Olathe (non-GM line) value was significantly different from Embrapa 5.1 (GM line) at p < 0.05.
Olathe DB are the values for the cv. Olathe within the Bean Databank
Table S2. Proximate analysis and anti-nutritional compounds in the transgenic bean line Embrapa 5.1 and its counterpart (cv. Olathe). Grains were harvested from plants grown under field conditions in three locations in 2009.
Component(mg/100g) / Londrina / Santo Antônio de Goiás / Sete Lagoa / Olathe DB / Databank
(reference range)
Olathe / Embrapa 5.1 / Olathe / Embrapa 5.1 / Olathe / Embrapa 5.1
Moisture / 9.56±0.56 / 10.01±0.48 / 7.17±0.31 / 8.38±0.30 / 9.41±0.22 / 10.95±0.22 / 9.23-13.85 / 9.23-14.78
Ash / 4.25±0.02 / 4.22±0.02 / 3.81±0.00 / 3.80±0.13 / 3.79±0.20 / 3.75±0.09 / 3.34-5.12 / 3.34-5.53
Protein / 18.99±0.15 / 19.01±0.16 / 19.03±0.26 / 19.29±0.10 / 21.31±0.04 / 23.36±0.05 / 14.20-27.2 / 13.46-27.20
Phytic acid / 7.47±±0.20 / 7.44±0.17 / 7.18±0.63 / 7.27±0.38 / 11.90±0.64 / 11.86±0.64 / 3.47-12.28 / 3.01-13.45
Trypsin inhibition / 199.8±1.4 / 198.4±1.0 / 205.5±5.7 / 184.6±18.8 / 193.1±5.0 / 174.2±6.7 / 101.9–295.2 / 101.9-392.7
Values are mean ± SEM. n= 3.
Moisture, Ash and Protein are in g/100g; Phytic acid is in mg/g and Trypsin inhibition is in TIU/100g.
Olathe DB are the values for the cv. Olathe within the Bean Databank
Embrapa 5.1 data compared with data of respective Olathe in the same location were not significantly different p < 0.05.
Table S3. Mineral composition of the transgenic bean line Embrapa 5.1 and its counterpart (cv. Olathe). Grains were harvested from plants grown under field conditions in three locations in 2008.
Element(mg/100g) / Londrina / Santo Antônio de Goiás / Sete Lagoas / Olathe DB / Databank
(reference range)
Olathe / Embrapa 5.1 / Olathe / Embrapa 5.1 / Olathe / Embrapa 5.1
Aluminum / 0.66±0.09 / 0.81±0.10 / 0.66±0.10 / 0.90±0.11 / 0.37±0.07 / 0.40±0.24 / 0.34-3.08 / 0.34-12.72
Calcium / 94.42±7.54 / 99.17±15.81 / 123.33±16.09 / 125.53±8.66 / 132.16±26.05 / 118.32±12.08 / 76.02-205.26 / 62.99-205.26
Copper / 0.50±0.06 / 0.55±0.05 / 0.46±0.07 / 0.43±0.05 / 0.33±0.06 / 0.29±0.06 / 0.49-1.32 / 0.49-1.48
Iron / 6.01±0.16 / 5.89±0.36 / 5.21±0.48 / 5.14±0.26 / 4.86±0.23 / 4.40±0.15 / 4.72-9.45 / 4.72-14.18
Phosphorous / 444.02±35.42 / 451.51±18.65 / 419.30±26.02 / 377.87±22.15 / 492.86±31.73 / 471.84±10.49 / 322.07-575.94 / 275.91-594.86
Magnesium / 171.33±5.04 / 174.00±6.40 / 182.79±2.54 / 175.64±7.01 / 157.46±4.16 / 154.87±2.39 / 146.84-215.84 / 135.71-230.36
Manganese / 0.57±0.11 / 0.66±0.04 / 0.60±0.06 / 0.77±0.17 / 0.29±0.07 / 0.31±0.02 / 0.72-2.37 / 0.71-3.19
Potassium / 1391.06±48.19 / 1459.33±55.54 / 1398.86±13.27 / 1384.97±16.50 / 1410.09±29.45 / 1395.62±16.20 / 972.89-1740.16 / 798.41-1766.16
Zinc / 2.01±0.12 / 2.12±0.14 / 2.15±0.17 / 1.96±0.15 / 2.23±0.11 / 1.98±0.05 / 1.97-3.73 / 1.76-15.25
Values are mean ± SEM. n= 3.
Olathe DB are the values for the cv. Olathe within the Bean Databank
Embrapa 5.1 data compared with data of respective Olathe in the same location were not significantly different p < 0.05.
Table S4. Mineral composition of the transgenic bean line Embrapa 5.1 and its counterpart (cv. Olathe). Grains were harvested from plants grown under field conditions in three locations in 2009.
Element(mg/100g) / Londrina / Santo Antônio de Goiás / Sete Lagoas / Olathe DB / Databank
(reference range)
Olathe / Embrapa 5.1 / Olathe / Embrapa 51 / Olathe / Embrapa 5.1
Aluminum / 1.04±0.89 / 1.47±0.37 / 2.62±0.58 / 3.67±0.82 / nd / nd / 0.34-3.08 / 0.34-12.72
Calcium / 131.78±27.57 / 126.56±22.26 / 165.34±8.12 / 160.56±16.17 / 137.30±7.96 / 134.83±6.76 / 76.02-205.26 / 62.99-205.26
Copper / 0.48±0.17 / 0.45±0.18 / 0.35±0.09 / 0.30±0.05 / 0.78±0.05 / 0.73±0.02 / 0.49-1.32 / 0.49-1.48
Iron / 5.87±0.87 / 5.84±1.11 / 6.71±0.46 / 7.44±0.68 / 6.64±0.19 / 6.52±0.36 / 4.72-9.45 / 4.72-14.18
Phosphorous / 439.67±54.24 / 419.35±55.79 / 366.96±16.63 / 323.80±16.62 / 480.28±28.59 / 461.23±17.42 / 322.07-575.94 / 275.71-594.86
Magnesium / 173.48±8.81 / 168.71±8.60 / 179.23±4.55 / 171.01±4.08 / 179.45±2.52 / 175.18±3.87 / 146.84-215.84 / 135.71-230.36
Manganese / 0.78±0.41 / 0.75±0.35 / 1.01±0.09 / 0.86±0.06 / 1.45±0.05 / 1.43±0.40 / 0.72-2.37 / 0.71-3.19
Potassium / 1421.09±87.22 / 1401.38±75.92 / 1350.21±23.94 / 1295.74±16.77 / 1582.50±29.70 / 1524.15±29.22 / 972.89-1740.16 / 798.41-1766.16
Zinc / 2.83±0.30 / 2.26±0.39 / 2.44±0.11 / 2.23±0.08 / 2.80±0.11 / 2.99±0.14 / 1.97-3.73 / 1.76-15.25
Values are mean ± SEM. n= 3.
Olathe DB are the values for the cv. Olathe within the Bean Databank
Embrapa 5.1 data compared with data of respective Olathe in the same location were not significantly different p < 0.05.
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