Chrysiogenes ------MRIKRREFLKASAAVGAVAVASPTLNAFAQTG-----TGASAMGEAEGKWIPST 49
Bacillus ------EQGEWIASV 9
Shewanella MKKENQVNLGRRQLLKSTAAGTVLTGIGGTLSFTPIVEGIAAELPAPLRRTGVGEWLATT 60
*:*:.:.
Chrysiogenes CQGCTTWCPVEFLFRMAVRSKYAATQLSKANNGYCCVRGHLMLQQLYDPDRIKTPMKRTN 109
Bacillus CQGCTAWCAVQVYRIDGRATKVRGNPNAKANHGHSCVRSHIGLQQVYDPDRVKQPMKRTN 69
Shewanella CQGCTSWCAKQIYVMDGRALKVRGNPNSGVHGMSSCPRQHLSLQQVYDPDRLRTPMMRTN 120
*****:**. :. . * .. : .: .***: ***:*****:: *****
Chrysiogenes PVKGRKEDPKICPYHMGMKQWDTIADKIMELRKNNETHKYLLMRGRYSDHNSIFYGDLTK 169
Bacillus PNKGRDEDPEFVPISW-EEAMDTIADKIIELRENNETHKFSVWRGRYTSNNGILYGNMPK 128
Shewanella PKKGRDQDPKFVPISW-DKALDMLADKIIALRVANEPHKYALLRGRYSHINDLLYKKMTN 179
****.:**:: * : * :****: ** **.**: : ****: *.::*.:.:
Chrysiogenes MIGSPNNISHSAICAEVEKMGSMATEGFWGYRDYDLDNMKYLIAWACDPLSSNRQIPNAI 229
Bacillus IIGSPNNISHSSICAESEKFGRYYTERYWGYADYDHENALYEIFWGGDPIATNRXVPHTA 188
Shewanella LIGSPNNISHSSVCAEAHKMGPYYLDGNWGYNQYDVKNAKFILSFGADPIASNRQVSFYS 239
:**********::***.*:* : *** :**.* : : :.**:::** :.
Chrysiogenes RKIQGVMDRGKVVAVDPRMNNTASKAQEWLPIKPSEDGALALAMAHVIITKGLWSKEFVG 289
Bacillus SIWGELSDRATLACVDPRFSTTAANQMNGCRLIPGEDGAIASAIAHVILTEGVWYKPFVG 248
Shewanella QTWGDSLDHAKVVVVDPRLSASAAKAHKWIPIEPGQDSVLALAIAHVALVEGVWHKPFVG 299
*:..:.****:. :*:: : : *.:*..:**:*** :.:*:*****
Chrysiogenes DFKDGKNKFVAGKTVKEEDFEEKLTNGIVKWWNLEVKDRTPKWAAKVTGIDEATIIRVAT 349
Bacillus DFKDGQNRFVEGRDVNEDDFEEIQTHGLVKWWNLELKDKTPEWAAERSGIDADQIYRVAR 308
Shewanella DFIEGKNLFKAGKTVSVESFKETHTYGLVEWWNQALKDYTPEWASKITGIDPKTIIAIAK 359
** :*:** *: *. :.*:* **:*:*** :****:**:: :*** * :*
Chrysiogenes EFAQAAPACAIWYGP--NMQPRGSYAVMCIHALNGLVGASDSEGGLCT-GMGSPSSSYPK 406
Bacillus GFANAAPKAISFSSPGSSMTIRGGYTAMTQAALNGLVGSADNLGGVISNGISVPNNGFPD 368
Shewanella DMGAAAPAVQVWTSRGAVMQARGTYTSISCHALNGLFGGIDSKGGLFPGNKTPLLKEYPE 419
:.*** : . * ***: : *****.*.*.**: .. . :*.
Chrysiogenes IDAYQDDVAKAGAKNKKIDQRGTLKFPAMGSAKPGTGVVTNNVADALLAADPYDIKVAIG 466
Bacillus PSDYIDEIAANGLEQDRIDWGGRLEFPALKDSKSGGVKVTNTVADAVLAEDPYEFKSRIE 428
Shewanella AKAYMDEIAAKGVKKEKIDQRGRLEFPALAKGKSGGGVITANAANGIRNQDPYEIKVMLA 479
.**::* * ::.:** **:***: ..*.* :*..*:.: ***::* :
Chrysiogenes YFCNFNFSGTDGARWDKALAKVPFFVHCVPMFSEMTYFADIVLPAALHHTEDWAVIR-SK 525
Bacillus LLDELQFLQPRNGSLGQSFGQDSIHGAYDCQSGEQTHFADIVLPVPHSQFERQSPVAGSN 488
Shewanella YFNNFNFSNPEGQRWDEALSKVDFMAHITTNVSEFSWFADVLLPSSHHMFEKWGVLD-SI 538
: :::* .. .:::.: : .* : ***::**. * . : *
Chrysiogenes ANLHGHTSIQQPVVERMFDVKGVETEITWLLAEKLKAKGFENMYNWLYNEYKDPETGKNP 585
Bacillus GNLHRHLHLQNKVIESPFDIRVDETEIPWMIGESLEKKGYSNLIDYFRNEFRDPETGEAP 548
Shewanella GNGVAQISIQQPSIKRLWDTRIDESEIPYMLAKKLADKGFDAPWRYINEQIVDPETGKPA 598
.* : :*: :: :* : *:**.:::.:.* **:. :: :: *****: .
Chrysiogenes TNSLEFALYATKIRSKKCWDPKENAEYKGDKLNGWADFMEKGIVNSPKFKFRQKWEKGFP 645
Bacillus TNAAEFDEIATKHYTHPVWDP--TFEKDGDQIDGWEEYKRLGTWNTNKYEFRQKWDGNWG 606
Shewanella ADEAEFAKLMVRYLTAPLWKE--DASKYGDKLSSWDEFVQKGVWNSSPYKLEARWGK-FK 655
:: ** .: : *. . **::..* :: .* *: :::. :* :
Chrysiogenes TETKKFEFYSETLKKGLLAHAEKNKVTVDQVMEATNYEARGELAFIPHYESPKRHGDVKE 705
Bacillus TETGQFEFYSETLKVALQDHADKHNASIDDVMEATFNTAKGELAFVPHYEPAMRVGDESE 666
Shewanella TETTKFEFYSKTLEKALQSHADKHKVSIDEVMKACDYQARGHLAFIPHYEEPYRFGDESE 715
*** :*****:**: .* **:*::.::*:**:* *:*.***:****.***.*
Chrysiogenes FPFSLIDMKSRLNREGRSTNATWYHAFKKCDPGDVNQEDVLQINPADAKKLGINEGDMVK 765
Bacillus YPLIFMEHRSKLNREARSANTSWYQEFKDIDLGDEAWDDVAKLNPKDAAELGIQNGDMVR 726
Shewanella FPLLLVDQKSRLNKEGRTANSPWYYEFKDVDPGDVANEDVAKFNPIDGKKFGLKDGDEIR 775
:*: ::: :*:**:*.*::*:.** **.*** :** ::***. ::*:::** ::
Chrysiogenes VTSVIGSLTVKARLWEGVRPGCVAKCYGQGHFAMGRVSAKDFGKAVARGANFNDIMPADY 825
Bacillus LVTPEGQIEVKAKLWEGTRPGVVAKCYGQGHWAYGHIASLDRRRQIARGGNNNIILAPVH 786
Shewanella ITSPVGMLTCKAKLWEGVRPGTVAKCFGQGHWAYGRYASAKFG-VTPRGGSNNDLIADRY 834
:.: * : **:****.*******:****:**: :: . .**..* ::. :
Chrysiogenes DRITGATARNGGFTGVKIEKA 846
Bacillus EALSGSGARHGGQTRVRVEKV 807
Shewanella DRLSGASAFYG-HIRVRVEKV 854
: ::*: * * *::**.
Figure 1S. Sequence conservation among ArrA proteins. ArrA sequences from C. arsenatis, B. selenitireducens, and Shewanella sp. strain ANA-3 were aligned using ClustalW (Chenna et al. 2003). Identical residues are indicated by an asterisk. Partially conserved residues are indicated by dots. The putative iron-sulfur cluster binding site and molybdopterin dinucleotide-binding domain (Saltikov and Newman 2003) are underlined in red and blue, respectively. Conserved regions used for the design of degenerate PCR primers that amplified a diagnostic region of arrA (Malasarn et al. 2004) are underlined in green.
References
1. Chenna, R., H. Sugawara, T. Koike, R. Lopez, T. J. Gibson, D. G. Higgins, J. D. Thompson. 2003. Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res 31(13): 3497-500
2. Malasarn, D., C. W. Saltikov, K. M. Campbell, J. M. Santini, J. G. Hering, D. K. Newman. 2004. arrA is a reliable marker for As(V) respiration. Science 306(5695): 455.
3. Saltikov, C. W. and D. K. Newman. 2003. Genetic identification of a respiratory arsenate reductase. Proc Natl Acad Sci U S A 100(19): 10983-8.