Characterization and Activity of Histone Deacetylase Genes in Aspergillus Flavus the Cause

Characterization and activity of histone deacetylase genes in Aspergillus flavus the cause of pistachio infection

1Hamed Fakhimhajiaghaei, 2Tahereh Basirnia ,3Kamyar Zomorodian, 4Hamid Badali

1Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran

2Department of Plant Pathology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

3Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

4Department of Medical Mycology and Parasitology, Mazandaran University of Medical Sciences,
Sari, Iran

Transcriptional regulation in eukaryotes occurs within a chromatin setting and is strongly influenced by posttranslational modification of histones.The regulation of histone acetyltransferases (HATs) and histone deacetylases(HDACs) is one of important post translational modification (PTMs).Aspergillus flavus is the main source of aflatoxin,the most important mycotoxins in the world and food supplies.

Here we describe the identification and sequencing HDAC genes and underastanding the possible effect of HDAC inhibitors on experession of HDAC genes in Aspergills flavus the pistachio pathogen.

Three HDACs genes were founded in this fungi ,HosA,HosB and Rpd1 that HosA and Rpd-1 were Rpd3-like class I and HosB was class II HDACs. Seguencing alignment display significant nucleotides similarity to other eukaryotic deacetylases. Real time RT-PCR analysis indicated that SAHA affectes experession of HDAC genes and decreased production of conidiophores, aflatoxin B1 and also causes a “fluffy” phenotype. HosA was sensitive against SAHA treatment whereas HosB showed resistance even at 50µM.

This results suggest that HDACs have important roles in A. flavus and have conserved domains like other fungi . HDACi could prove to be a valid approach for selective treatment of infection cause by Aspergillus species and other fungi and be a good subject for epigenetic remodeling in fungi. These experimental data suggest that HDACis could prove to be instrumental in developing new therapies for fungal infection.

Biography

Hamed Fakhim has PhD student in medical mycology Department of Medical Mycology and Parasitology, Mazandaran University of Medical Sciences, Sari, Iran.

Presenting author details
Full name: Hamed Fakhimhajiaghaei
Contact number: +989144117308
Twitter account:
Linked In account:

Session name/ number:
Category: (Poster presentation)