ESM 1

Gene /

Query

/ Subject / Bin map position / wEST clone / E value / Score / Nucleotide Identity / Co-location with disease resistance loci or QTL in wheat / Wheat Pathogen / Reference
LpCAT / Perennial ryegrass EST
Perennial ryegrassEST
Unmapped wheat wEST
(wESTBJ237185) / Mapped wEST (TaCAT)
Unmapped wEST
Mapped wEST / 4BL, 4DL, 5AL, 6BS
N/A
4BL, 4DL, 5AL, 6BS / BF293613
BJ237185
BF293613 / 1 x 10-37
0
5 x 10-93 / 319
940
337 / 95%
95%
98% / QTLs on 4DL / Leaf rust
Leaf tip necrosis / Messmer et al.(2000)
LpOXO / Perennial ryegrassEST
Perennial ryegrassEST
Unmapped wEST
(wESTCA743092) / Mapped wEST (TaOXO)
Unmapped wEST
Mapped wEST / 4BS, 4DS
N/A
4BS, 4DS / BE405592
CA743092
BE405592 / 5 x 10-62
1 x 10-116
6 x 10-86 / 234
420
313 / 86%
88%
88% / QTLs on homoeologous group 4 chromosomes / Tan spot
Leaf rust / Faris et al.(1999)
LpPERa / Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wESTBJ283317) / Mapped wEST (TaPERa)
Unmapped wEST
Mapped wEST / 2AS, 2BS, 2DS
N/A
2AS, 2BS, 2DS / BE407078
BJ283317
BE407078 / 3 x 10–34
1 x 10-126
3 x 10-54 / 143
456
208 / 81%
88%
83% / QTLs, Lr23 locus on 2BS / Leaf rust / Faris et al. (1999)
LpERa / Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wESTBE445264) / Mapped wEST (TaERa)
Unmapped wEST
Mapped wEST / 2AL, 2BL, 2DL
N/A
2AL, 2BL, 2DL / BF201830
BE445264
BF201830 / 1x10–65
9x10–64
0 / 246
246
888 / 82%
82%
100% / Yr5 and Yr7loci on 2AL / Stripe rust / Kuraparthy et al. (2007)
LpTHb
LpGLR
LpNOX
LpDEFa
LpTHc
LpGLUCk
LpCHIjb
LpTLa
LpWRKY14 / Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST BG263261)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST BE517773)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST BJ314502)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST CD886145)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST BQ619678)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST BJ270297)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST CD866067)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST CD869050)
Perennial ryegrass EST
Perennial ryegrass EST
Unmapped wEST
(wEST BJ268847) / Mapped wEST (TaTHb)
Unmapped wEST
Mapped wEST
Mapped wEST (TaGLR)
Unmapped wEST
Mapped wEST
Mapped wEST (TaNOX)
Unmapped wEST
Mapped wEST
Mapped wEST (TaDEFa)
Unmapped wEST
Mapped wEST
Mapped wEST (TaTHc)
Unmapped wEST
Mapped wEST
Mapped wEST (TaGLUCk)
Unmapped wEST
Mapped wEST
Mapped wEST (TaCHIjb)
Unmapped wEST
Mapped wEST
Mapped wEST (TaTLa)
Unmapped wEST
Mapped wEST
Mapped wEST (TaWRKY14)
Unmapped wEST
Mapped wEST / 1BS, 1DS
N/A
1BS, 1DS
3AS, 3BS, 3DS
N/A
2AL, 2BL, 2DL
6AL
N/A
5AL
1BS, 1DS
N/A
1BS 1DS
3BL, 7AS, 7BS, 7DS
N/A
2BL
7BL
N/A
N/A
3AL 3BL 3DL
N/A
2BL 2DL
5BL
N/A
5BL
5AL, 5BL
N/A
6AS, 6BS, 6DS / BF429344
BG263261
BF429344
BE404656
BE517773
BF482280
BF483133
BJ314502
BF291638
BF429344
CD886145
BF429344
BE499303
BQ619678
BE406665
BF291392
BJ270297
N/A
BE500112
CD866067
BF428678
BE604419
CD869050
BE604419
BG313955
BJ268847
BE500768 / 6 x 10–18
3 x 10–26
2 x 10-64
0.34
0
1.1
0.11
1 x 10-100
1 x 10-07
1 x 10–49
7 x 10–55
6 x 10-98
0.2
3 x 10–19
0.006
0.36
0.12
N/A
8 x 10 –07
2 x 10–11
0
3 x 10-34
3 x 10-67
4 x 10-10
0.039
7 x 10-43
0.025 / 87.7
121
242
32.2
658
30.2
34
367
54
192
216
353
32.7
97.6
22
27
N/A
50.1
71
650
132
144
62
36
109
45 / 85%
85%
87%
100%
91%
100%
100%
91%
89%
85%
85%
88%
100%
84%
96%
100%
84%
N/A
96%
79%
96%
86%
88%
90%
76%
91%
82% / Lr21 locus on 1DS
N/A
N/A
Lr21 locus on 1DS
N/A
N/A
N/A
Lr1 locus on 5DL
N/A / Leaf rust
N/A
N/A
Leaf rust
N/A
N/A
N/A
Leaf rust
N/A / Huang et al. (2003)
N/A
N/A
Huang et al. (2003)
N/A
N/A
N/A
Ling et al. (2004)
N/A

ESM 2

(a)

CGCCGACTTCCTCCGCGCC-CTGGCGTCCAGACCCCCGTCATCGTCCGCTTCTCCACCGT LpCAT

|||||||||||||||||| | || || |||||||||||||| || ||||||||||||||

CGCCGACTTCCTCCGCGCGTCGGGGGTGCAGACCCCCGTCATAGTGCGCTTCTCCACCGT TaCAT

CATCCACGAGCGCGGCTCCCCCGAGACCCTCCGCGACCCTCGCGGGTTCGCCATCAAGTT LpCAT

|||||||||||||||||||||||||| ||||||||||| ||||| ||||||||||||||

GATCCACGAGCGCGGCTCCCCCGAGACGCTCCGCGACCCGCGCGGCTTCGCCATCAAGTT TaCAT

CTACACCCGGGAGGGCAACTGGGACCTGGTCGGCAACAACTTCCCGGTCTTCTTCATCCG LpCAT

|||||||||||||||||||||||||||||| |||||||||||||| ||||||||||||||

CTACACCCGGGAGGGCAACTGGGACCTGGTGGGCAACAACTTCCCCGTCTTCTTCATCCG TaCAT

CGACGGCATGAAGTTCCCGGACATGGTGCACGCGCTCAAGCCCAACCCCAAGACCCACAT LpCAT

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

CGACGGCATGAAGTTCCCGGACATGGTGCACGCGCTCAAGCCCAACCCCAAGACCCACAT TaCAT

CCAGGAGAACTGGCGCGTGCTGGACTTCTTCTCGCACCACCCGG LpCAT

||||||||||||||| | || ||||||||||||||||||||||

CCAGGAGAACTGGCGGATCCTCGACTTCTTCTCGCACCACCCGG TaCAT

(b)

GCGTGTCCATGAACCGTGTCGACTTCGCGCCGGGTGGCACGAACCCACCGCACATCCACC LpOXO

||||||| ||||||||||| |||||||||||||| ||||| ||||| || ||||||||||

GCGTGTCGATGAACCGTGTGGACTTCGCGCCGGGGGGCACCAACCCGCCACACATCCACC TaOXO

CGCGCGCTACCGAGATTGGCATCGTGATGAAAGGTGAGCTTCTCGTCGGAATCATCGGCA LpOXO

| || || |||||||| |||||| |||||||||||||||| ||||| |||||| | ||||

CCCGTGCCACCGAGATCGGCATCATGATGAAAGGTGAGCTCCTCGTTGGAATCCTTGGCA TaOXO

GCCTCGACTCTGGGAACAAGCTATACTCCAAGG--TGTTGCGTGCTGGTGAGACATTCCT LpOXO

|||| ||||| ||||||||||| ||||||| || | |||| ||||| ||||| |||||

GCCTTGACTCGGGGAACAAGCTCTACTCCAGGGGTGGGTGCGCGCTGGGGAGACGTTCCT TaOXO

CATCCCGCGTGGGCTCATGCACTTTCAGTTCAACGTTGGCAAGACGGAGGCATCCATGGT LpOXO

||||||||| || ||||||||||| ||||||||||| || ||||| || || ||||||||

CATCCCGCGCGGCCTCATGCACTTCCAGTTCAACGTCGGTAAGACCGATGCTTCCATGGT TaOXO

TGTGTCCTTCAACAGCCAGAACCCAGGCATCGT LpOXO

|| |||||||||||||| ||||| ||||||||

CGTCTCCTTCAACAGCCAAAACCCCGGCATCGT TaOXO

(c)

TCGCGACATCTTTCAGGGCCA-CTGCCCCCAGTCCGGCGGCGACAGCAGCCTGGCGCCGC LpPERa

||||||| || ||| ||||| |||||| |||||||||||| || ||| ||||||| |||

TCGCGACCTCGCTCAAGGCCAACTGCCCGCAGTCCGGCGGCAACGGCAACCTGGCGGCGC TaPERa

TGGACACAGCGACGCCCAATGCGTTCGACAACTCCTACTACACCAACCTCATGTCCCAGA LpPERa

||||| | |||| |||| || ||||||||| ||||||||||| |||| | |||||||

TGGACGCCACGACCGCCAACGCCTTCGACAACGCCTACTACACCGACCTGCTCTCCCAGA TaPERa

AGGGGCTGCTTCACTCGGACCAGGTGCTGTTCAATGGCGGCGGCGCCGACAACACGGTCA LpPERa

||||||| || || || ||||||||||| ||||| || | | |||||||||| ||||

AGGGGCTCCTCCATTCCGACCAGGTGCTCTTCAACAACGCCACCACCGACAACACCGTCA TaPERa

TGAGCTTCGCGACCAGCGCGGCCACGTTCAACAGCGCCTTCACGACGGCCATGATAAACA LpPERa

|| ||||||| |||||| ||| | |||| |||||||||| | ||||||||| | || |

GGAACTTCGCGTCCAGCGGGGCGGCCTTCAGCAGCGCCTTCGCCACGGCCATGGTCAAGA TaPERa

TGGGAAACATCGCGCCAAAGACGGGGACGCAGGGGCAGATCAGGCTCGTCTGCTCCAAGG LpPERa

|||| |||||| |||| || ||||||||||| |||||||||||| |||||||||||

TGGGCAACATCTCGCCGCTCACAGGGACGCAGGGCCAGATCAGGCTCAGCTGCTCCAAGG TaPERa

TCAACTCGT LpPERa

| |||||||

TGAACTCGTTaPERa