GC-MS studies of phytochemical composition in hexane extract ofDracocephalum kotchyiBOISS. Leaves

M. Basiri 1; A. Khanizade 2 and S. Rashidi Monfared1

1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Islamic Republic of Iran

2Life environment keeping organization, Aleshtar, Lorestan , Islamic Republic of Iran

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Abstract

The members of the Lamiaceae L. Family contain a complex of biologically active compounds and display diversified pharmacological activities and low toxicities. Dracocephalum kotchyiBOISS, which belongs to the plant family Labiatae, is the important endemic perennial herb species of Iran. It’s used as an anti-inflammatory and sedative drug for treatment of conjunctivitis and keratitis, Dry herb has been used to treat heart disease, blood pressure, angina, tracheitis, atherosclerosis, neuralgia, migraine, and headache and toothache. Here we study the possible compounds of D. kotchyiBOISS. Leaves n-hexan extracts and compare four different ecotypes by GC-MS analysis. Gas chromatography mass spectrometry (GC-MS) has become a key technological platform for secondary metabolite profiling in both plant and non-plant species. Fifteen compounds were identified in the extract of collectedherbs. UPGMA cluster analysis of four ecotypes based on metabolite content shows a cophenetic coefficient, DKA, DKB and DKD are in the first cluster and DKC is placed in the second cluster alone. Among fifteen compounds, almost in all ecotypes, the major compounds were detected, including: Hentriacontane, Vitamin E, Bis (2-ethylhexyl) phthalate, Docosane, Triacontane and Squalene. Phytols, Squalene, Hentriacontane and Vitamin E have different and distinct pharmacological activities. The largest amount of Phytol, Squalene, HenTriacontane and Vitamin E were belonging to the ecotypes of DKA (0.628 %), DKD (1.709%), DKB (12.686%) and DKA (3.638%) respectively, and lowest amount of all these compounds were belongs to DKC (0.182%, 0.388%, 3.899% and 0.649% of totals).

Keywords:GC-MS analysis, Dracocephalum kotchyi BOISS., Leaves extract

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