Name______

Biology Blood Transfusion Lab

PURPOSE: To demonstrate the concepts involved in blood transfusions and to form conclusions about blood donors and receivers.

MATERIALS:

* depression plate* plain water- type O* red food coloring – type A

* 4 medicine droppers* blue food coloring – type B* red and blue (mixed) – type AB

* 4 test tubes* test tube rack

PROCEDURE AND OBSERVATIONS:

  1. Label the four test tubes A, B, AB, and O.
  2. Place 4 mL of red food coloring from the type A beaker into test tube A. *4mL is about the widthof your fingers
  3. Place 4 mL of blue food coloring from the type B beaker into test tube B.
  4. Place 4 mL of the mixed food coloring from the type AB beaker into test tube AB.
  5. Place 4 mL of water into test tube O.
  6. Place a depression plate on a piece of paper and label on the paper the following above each
    column of depressions A, B, AB, O. Place 4 drops from test tube A onto four depressions in a
    row on theclean depression plate as shown in the picture below.

(Receivers)
A B AB O

  1. Notice the data table on the other side of this paper. The drops on the plate represent bloodreceiver types. The colored water in each test tube represents the blood donor types.
  1. Using the dropper from each test tube, combine four drops from each donor tube with four dropson the type A receiver depression plate.
  1. If a combination produces a color change, the transfusion would not be safe. If there isn’t a colorchange, that indicates a safe transfusion. Record your observations in the data table by writing safe or unsafe in each box.
  1. After recording your observations for the type A receiver plate, rinse the plate off and dry it.
  1. Now place 4 drops from test tube B onto the four depressions on the plate.
  1. Using the dropper from each test tube, combine four drops from each donor tube with four dropson the type B receiver depression plate.
  1. Record your observations as you did for the type A receiver plate.
  1. Repeat the same procedure for type AB and O receiver plates.

DATA TABLE

Write SAFE in the box for a negative reaction (no color change).

Write UNSAFE in the box for a positive reaction (color change).

RECEIVER
D
O
N
O
R / A / B / AB / O
A
B
AB
O

1. What types of blood can a type A receiver be safely given in a transfusion?

2. What types of blood can a type B receiver be safely given in a transfusion?

3. What types of blood can a type AB receiver be safely given in a transfusion?

4. What types of blood can a type O receiver be safely given in a transfusion?

5. Type A blood has typeantigens and antibodies.

6. Type B blood has typeantigens andantibodies.

7. Type AB blood has typeantigens and antibodies.

8. Type O blood has antigens and antibodies.

9. Which blood type might be considered a “universal donor”? EXPLAIN ______

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10. Which blood type might be considered a “universal receiver”?EXPLAIN

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Source: www2.informns.k12.mn.us/.../585666Blood_Tranfusion_Lab.doc