Biotechnology Fall Exam Review 2008

Biotechnology Introduction

  1. Define Biotechnology
  1. How has biotechnology changed through the decades?

Legal-Scientific Notebook

3.Why is it important to keep a scientific notebook in a laboratory?

4. How is a scientific notebook set-up and kept on a daily basis?

Safety

5.What are MSDS data sheets and what information to they contain?

6. What are some safety “do’s and don’ts” in a laboratory?

Scientific Methodology

7. Describe the characteristics of a good hypothesis.

8. What are the components of a valid experiment?

9. What does the following components of the analysis mean: “REE, PE, PA”?

Inorganic Chemistry

10.How do you find the following, using the Periodic Table?

Atomic number, atomic weight, # protons, # neutrons, # electrons

11. Describe chemical bonding in the water molecule. How does it relate to the

characteristics of water?

12. Describe the following chemical bonds: hydrogen, ionic, polar covalent, non-polar

covalent.

13. What is meant by the term “dissociation” as it relates to ionic bonding?

14. What are H+ and OH-?

15. What is the definition of an acidic substance?

16. What is the definition of a basic substance?

17. Describe acids and bases in terms of hydrogen and hydroxide ions.

18. What is a buffer and how does it function?

19. Describe the pH scale. What does it measure?

20. What is electrolysis? Why is it important concept to know for biotechnology?

Organic Chemistry

20. What is Biuret’s solution? It changes from blue to ______in the presence of a ______.

21. In the cheese-making lab, what was the independent variable? dependent variable?

22. What are chymosin and rennin?

Measurement in the Laboratory

23. Describe how you would convert a number to scientific notation.

24. How do you convert larger metric units to smaller units? Smaller to larger?

25. What are the metric prefixes for 100, 10-3, 10-6, 10-9,10-12, 10-15

26. What are the rules for using a micropipet? Draw pictures of this process.

27. How do you read the following micropipets? P-10, P-20, P-200, P-1000. Draw diagram of a sample reading for each and label the units for each number.

P-10
1
5
4

28. List the appropriate volumes/masses that each of the following measure.

p-20, p-200, p-1000, 10 ml pipet, 1 ml pipet, graduated cylinder, analytical balance, table top balance

29. What is the major rule when loading a centrifuge? diagram

30. What is the difference between an analytical balance and Ohaus table top balances used in biology class?

31. What is a spectrophotometer? Be able to label and identify parts and function of each.

32. Describe the steps to using a spectrophotometer in biotech class.

33. What is the Beer-Lambert Law and how does it apply to biotechnology? Diagram a picture to demonstrate this Law.

Solutions/Dilutions

34.List the formulas for each of the following types of solutions:

mass/volume:

% mass/volume:

molarity:

35. How is formula weight determined?

36. What is a mole of a substance?

37. Define molarity:

38. What is the correct formula for making dilutions?

39. What are the components of a 5 ml of a 50 mg/ml copper sulfate pentahydrate solution?

40. What is the correct formula for determining the mass of NaCl needed for 5 mL of a 0.1% NaCl solution

41. How much NaCl is needed to make 50ml of a 0.5M NaCl solution.?

42. Given the following solution, determine the mass/volume concentration,

% mass/volume concentration, and molarity,. Show all your calculations.

3 mg NaCl in a total of 100 ml of water

43. How would you make 60ml of a 1X NaCl solution from a 20X NaCl solution?

44. Suzie made a solution by putting 0.25g of copper sulfate pentahydrate in a graduated cylinder and water to make a total volume of 50 mL.

  1. What is the mass/volume concentration of this solution?
  1. What is the %mass/volume concentration of this solution?
  1. What is the molarity of this solution?
  1. Assuming Suzie’s solution was 50X concentration, what calculations would you do to make 100 mL of a 1X solution
  1. What would be the components of this dilution?

Etcetera

  1. What are the 4 Macromolecules and their functions?
  1. Define qualitative and quantitative data AND give specific examples of each.
  1. What is the purpose of the fume hood?
  1. Where do you dispose of your lab waste? Give examples of 4 types of waste and how they are disposed?

Fall 2008 Biotech 1 Review Guide1