Biology 270: Microbiology
Tentative Lab Schedule – Monday Wednesday
Fall 2011
DATETOPIC/EXERCISEASSIGNMENTS
MondayStudent Data Sheets-hndtStudent data sheets
Aug 15Rules/Procedures/Safety-hndtLB: Lab Safety p. ix
Microscope use & care-hndtTxtbk: pp 93-98
Specimen size determination-hndt
Ex 1 Microscope - complete
Letter e – wrksht
Safety walk-through – Discussion
Wednesday
Aug 17Ex 8 Aseptic technique –
Plate to slantTxtbk: Chapter 22
Slant to plateCoat required
Slant to broth
Discuss Aseptic technique
Ex 12 Negative Stain – complete; prepared slide
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MondayEx 10 Smear Prep – completeTxtbk: pp 106-112
Aug 22Ex 11 Simple Stain – complete; prepared slide
WednesdayEx 14 Gram Stain - complete
Aug 24Ex 13 Capsule Stain – complete prepared slide
Use procedure on Board not in LB
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Monday
Aug 29Ex 15 Spore Stain – complete; prepared slide
Ex 16 Acid Fast Stain – complete; prepared slide
Wednesday
Aug 31Ex 17 Motility – wet mount, hanging drop and motility stab
End of material for exam 1
Ex 9 Pure Culture – beginTxtbk: pp 176-178
- streak for isolation
- pour plate
Ex 30 UV light – begin
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Monday Holiday
Sept 5
WednesdayEx 9 Pure Culture – subcultureTxtbk: pp 61-64
Sept 7 Ex pH – Deck 1 - Alcaligenes
Deck 2 - Saccharomyces
Deck 3 - Staph
Ex 20 Enumeration of Bacteria – SPC, TurbidityTxtbk: pp 187-188, 191-192
Readings
Ex 17 Motility – finish; ck motility stabs …………………….……………………………………………………………………………………………………………………………………………………
MondayExam 1
Sept 12Discussion of Conc/Dil – study guide hndt
Discussion of Spectrophotometer – hndt
OD/Trans Calculations – hndt
How to use logs – hndt
WednesdayNo class
Sept 14
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MondayEx 20 Enumeration – Standard Curve; discussion
Sept 19Conc/Dil problems – hndt
Ex 34 Antiseptics – collect mouth organisms
Ex 9 Pure Culture – gram stain, finish
Ex. 30 UV light finish
pH collect class data
WednesdayEx 34 Antiseptics – Begin
Sept 21Ex 26 Temp: Growth – begin
Deck 1 - Pseudomonas fluorescens
Deck 2 – Serratia marcescens
Deck 3 – Bacillus stearothermophilus
Ex 35 Hand Scrubbing – begin
Ex 33 Antimicrobials
Deck 1 – Pseudomonas aeruginosa
Deck 2 – Enterococcus faecalis
Deck 3 – Staph aureus
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MondayEx 33 Antimicrobials – collect class data
Sept. 26Ex 35 Hand Scrubbing – collect class data
Ex 26 Temp – collect class data
Wednesday
Sept 28Discussion of Bergey’s Manual; Journal; Descriptive Chart
Practice flow chart exercise – hndt
Ex 34 Antiseptics- collect data
End of material for exam 2
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MondayUnknown Ex 36-40
Oct 3A. Gram Stain – check off with lab staff
B. Inoculate:
1. 2 TSA slants; use straight line for pattern of growth; stocks
2. 3 TSB – pattern of growth and opt temp
3. Thio – O2 requirement
4. motility agar – incubate 25oC (Room temp)
5. TSA plate – colony morphology, catalase, oxidase
6. Blood agar – hemolysis
WednesdayExam 2
Oct 5Read:
1. patterns of growth on
a. slant
b. plate
c. broth
2. hemolysis on blood agar
3. motility stab
4. opt temp – read % transmission of spec of broths
5. catalase & oxidate on TSA plate – go to lab window
6. O2 requirment in thio
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MondayUnknown Ex 36-40
Oct 10A. Gram Stain – ck for purity and make new stock
B. Inoculate:
1. Simmon’s citrate – carbon source
2. nitrate – nitrate reduction
3. spirit blue agar – lipase
C. Stains:
1. spore stain if necessary
2. Acid fast if necessary
3. capsule
4. negative is necessary
5. mixed smear for size determination
WednesdayUnknown Ex 36-40
Oct 12A. Gram stain – ck for purity and make new stockDescriptive Chart #1 with Flow
B. Inoculate: All require 5 day incubations Chart to group on back
1. starch agar plate - amylase
2. gelatin stab – gelatinase
3. MR-VP broth -methyl red test = mixed acid fermentation of glucose
4. urea broth – urease
5. skim milk agar plate – caseinase
C. Read:
1. Simmon’s citrate agar slant for citrate carbon source
2. nitrate reduction
3. spirit blue agar plate for lipase
MondayUnknown Ex 36-40
Oct 17A. Gram stain – ck for purity and make new stock
B. Inoculate: 2 day incubations
1. MR-VP broth for Voges-Proskuaer test
2. decarboxylation broths – lysine, ornithine, arginine
3. SIM stab – H2S production and tryptophan hydrolysis (indole)
4. DNase
5. phenylalanine- deamination
6. fermentation broths –
7. ferm-oxid glucose 2 per student
C. Read:
1. starch - amylase
2. gelatin - gelatinase
3. MR test – mixed acid fermentation
4. urea broth - urease
5. skim milk agar plate – caseinase
TuesdayREAD FERMENTATION BROTHS
Oct 18
WednesdayUnknown Ex 36-40Descriptive chart #2 with flow
Oct 19A. Gram stain for purity ck and make new sock if necessarychart to genus on back
B. Inoculate in necessary for
1. any special test
2. any drug sensitivity tests
C. Read:
1. Voges Proskuaer test for fermentation of glucose to Butanediol
2. decarboxylase broths
3. SIM for indole & H2S production
4. DNase plae
5. phenylalanine deamination
6. fermentation broth
7. ferm-oxid glucose broths
D. Ex 47 Water Exam – presumptive test
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Monday Ex 47 Water Exam – ConfirmedRead LB Ex 52; no report
Oct 24 pGLO
WednesdayEx 47 Water Exam – completed testUnknown Report
Oct 26pGLO finish
Elisa test – complete; discussion
End of material for exam 3
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MondayExam 3
Oct 31Ex. 47 finish collect data
Lab drawing guidelines – hndt
Invertebrates drawing guidelines – hndt
Taxonomy of Animals
WednesdaySurvey of Invertebrates – prepared slides
Nov 2Life cycles
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MondaySurvey of Invertebrates – prepared slides
Nov 7
WednesdaySurvey of Invertebrates
Nov 9Life cycles
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MondaySurvey of Invertebrates
Nov 14Life cycles
WednesdaySurvey of Invertebrates
Nov 16Life cycles
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MondaySurvey of Protozoa
Nov 21Life cycles
WednesdaySurvey of Protozoa
Nov 23Life cycles
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MondaySurvey of Protozoa
Nov 28Life cycles
WednesdaySurvey of Fungi
Nov 30
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MondaySurvey of Fungi
Dec 5
WednesdayLab final-Parasites
Dec 7