BIOL 235 - Microbiology I

Fall 2011

Lecture / Instructor / Office / Office Hours / Phone / Email
Mon 2:00-3:50
Crawford Hall Room#103
Tues 2:00-2:50
Crawford Hall
Room #107 / Professor C. Thompson / 247
CrfHl / Tues 11:00 – 1:00
Wed 1:00 – 2:50
Fri 11:00 - Noon / 6136 /

COURSE DESCRIPTION: The biology of microscopic organisms including bacteria, fungi, protozoa, algae, and viruses. An introduction to basic principles of microbiology, with emphasis on morphology, classification, cultivation, growth, physical & chemical controlling agents, antibiotics, host-parasite interactions, and the benefits of microorganisms including genetic engineering applications.The lab includes proper technique in the observation and identification of microbes, and reactions under various physical and chemical conditions.

Prerequisite: Biology Placement BIOL 120 – General Biology I or Successful completion of college-level biology (ex. BIOL 105) with at least a C-.

4 credits (3 lecture hours, 2 laboratory hours)

LAB: OSHA regulations for personal safety. If this is not adhered to you will be dismissed from lab.

  • NO FOOD or BEVERAGES of any kind.
  • Students MUST WEAR the provided LAB COAT
  • Students MUST WEAR safety goggles when instructed.

REQUIRED:

  • Prescott’s Microbiology, 8th ed., 2011, Willey/Sherwood/Woolverton, McGraw Hill
  • Companion Site Learning Center – Student Edition at
  • Custom Lab Manual, Harley, McGraw-Hill
  • Notebook to take notes in both lecture and lab
  • 3-ring notebook or binder

PROVIDED: Lab Coat - Safety Goggles- Microscope slides - Wax Marking pencil

LAP TOP COMPUTERS are not required for this course. In the event that a lab top computer is used any inappropriate use will be dealt with on an individual basis.

CELL PHONES, iPODS, HEADSETS and other ELECTRONIC DEVICES: Prior to entering lecture or lab all electronic devices must be turned off and put away. (unless you are recording the lecture to help you study). Headsets are to be removed.

CIVILITY:

  • Each student is expected to be courteous to fellow students and the instructor.
  • There is to be no talking when the instructor is speaking or students are asking questions.
  • Students are not to arbitrarily leave and come back to class during lecture or lab.
  • When graded papers are passed back, students are to respectfully discuss any questions privately with the

instructor.

TUTORS: No tutors are available during the summer session.

STUDENTS WITH DISABILITIES: Please contact Mr. David Symonds, at the AEC in the Library, to discuss special accommodations. No special accommodations will be provided until the instructor has been notified by Mr. Symonds.

ATTENDANCE POLICY:

According to the Faculty & Staff Handbook of Morrisville State College, each student is expected to attend all scheduled classes and laboratories.

Lecture

  • Each TUESAY there will be either a quiz or an exam. Quizzes will be administered towards the end of class.
  • Students may not come to lecture late to just take a quiz; if so it will be counted as a zero.
  • If an absence is unavoidable, it is the student's responsibility to obtain the material (notes) missed.
  • If an absence is unavoidable on an exam day you are to contact the instructor by 5:00 pm that day to make arrangements to take the exam, before it is passed back.
  • There are NO make-ups for any missed quizzes. One lecture quiz will be dropped to accommodate an unavoidable absence.

Lab

  • Students may not come to LAB to just take a quiz and leave; if so it will be counted as a zero.
  • Students are not to leave lab when instructions are given.
  • If an absence in LAB is unavoidable, it is the student's responsibility to notify the instructor immediately so arrangements can be made to attend another lab section. You may NOT make up more than two (2) labs during the semester this way.

LAB Sections / Room / Day / Time
01L / Crawford Hall #257 / Monday / 4:00 – 5:50 p.m.
02L / Crawford Hall #257 / Tuesday / 3:00 – 4:50 p.m.
03L / Crawford Hall #257 / Wednesday / 3:00 – 4:50 p.m.
  • The lowest lab quiz and lab report grade will be dropped at to compensate for an unavoidable absence.
  • If you miss a week’s lab you are responsible for reading over the lab missed and ask any questions

beforehand in preparation for the quiz that will be administered the following week.

  • If you miss a week’s lab you are to submit the lab report that was due during your absence to your lab instructorby the next lab period.
  • Papers submitted without a name will receive a zero
  • You must be in lab and perform the lab experiment in order to turn in the lab report for that exercise.

ACADEMIC HONESTY:

  • Keep in mind that assignments are given to allow you to demonstrate what you have learned in class as well as show off your own creativity.
  • Each assignment is therefore expected to be your own original work.
  • Any work that is determined by the instructor to be dishonest in any form will be treated according to the standards for Academic Honesty as described in Morrisville State College Student Handbook and Faculty/Staff Handbook

Academic dishonesty

From Wikipedia, the free encyclopedia

Fabrication is the falsification of data, information, or citations in any formal academic exercise. This includes making up citations to back up arguments or inventing quotations. Fabrication predominates in the natural sciences, where students sometimes falsify data to make experiments "work". It includes data falsification, in which false claims are made about research performed, including selective submitting of results to exclude inconvenient data to generating bogus data.

There is also the practice ofdry-labbing—which can occur inchemistryor other lab courses, in which the teacher clearly expects the experiment to yield certain results (which confirm established laws), so the student starts from the results and works backward, calculating what the experimental data should be, often adding variation to the data. In some cases, the lab report is written before the experiment is conducted—in some cases, the experiment is never carried out. In either case, the results are what the instructor expects.

Deception is providing false information to a teacher/instructor concerning a formal academic exercise. Examples of this include taking more time on a take-home test than is allowed, giving a dishonest excuse when asking for a deadline extension, or falsely claiming to have submitted work. This type of academic misconduct is often considered softer than the more obvious forms of cheating, and otherwise-honest students sometimes engage in this type of dishonesty without considering themselves cheaters. It is also sometimes done by students who have failed to complete an assignment, to avoid responsibility for doing so.

Cheating can take the form of crib notes, looking over someone's shoulder during an exam, or any forbidden sharing of information between students regarding an exam or exercise. Many elaborate methods of cheating have been developed over the years. For instance, students have been documented hiding notes in the bathroom toilet tank, in the brims of their baseball caps, or up their sleeves. Also, the storing of information in graphing calculators, pagers, cell phones, and other electronic devices has cropped up since the information revolution began. While students have long surreptitiously scanned the tests of those seated near them, some students actively try to aid those who are trying to cheat. Methods of secretly signaling the right answer to friends are quite varied, ranging from coded sneezes or pencil tapping to high-pitched noises beyond the hearing range of most teachers. Some students have been known to use more elaborate means, such as using a system of repetitive body signals like hand movements or foot jerking to distribute answers (i.e. where a tap of the foot could correspond to answer "A", two taps for answer "B", and so on).

Cheating differs from most other forms of academic dishonesty, in that people can engage in it without benefiting themselves academically at all. For example, a student who illicitlytelegraphedanswers to a friend during a test would be cheating, even though the student's own work is in no way affected. Another example of academic dishonesty is a dialogue between students in the same class but in two different time periods, both of which a test is scheduled for that day. If the student in the earlier time period informs the other student in the later period about the test; that is considered academic dishonesty, even though the first student has not benefited himself.

Impersonation is a form of cheating whereby a different person than the student assigned an assignment or exam completes it. Unlike in Cheating, the academic work is totally 'outsourced' to another person or organization, usually for pay

GRADING POLICY:

  • Grades are NOT negotiable.
  • There is NO extra credit; Grades are based on quality not quantity.
  • Grades are not curved.
  • Students may not attend Lab or Lecture to just take a quiz and leave (if so it will be counted as a zero).
  • Grades are EARNED by the quality of knowledge you learned from lab and lecture as demonstrated byquizzes, exams, and lab reports.

% of Lecture Grade% of Lab grade

Quizzes30Lab Reports30

Exams45Quizzes45

Final Exam25Unknown25

Lecture – 75% of course grade:

Your lecture grade will comprise 75% of your BIOL 235 course grade and will be computed by taking 75% of your exam average, and 25% of your final exam grade. These values together to give you a numerical grade for lecture, which is then multiplied by 0.75 (75%) and added to 25% of your lab grade.

Example:Quiz average80 x 0.30 = 24.00

Exam average 75 x 0.45 = 33.75

Final exam70 x 0.25 = 17.50

75.25 = C+

Lab – 25% of course grade:

Your lab grade will comprise 25% of your BIOL 235 course grade and will be computed by taking 30 % of your lab report average, 45% of your quiz average, and 25% of your grade earned on the determination of an unknown bacterial culture. These values together to give you a numerical grade for lab, which is then multiplied by 0.25 (25%) and added to 75% of your lecture grade.

Example:Lab Quiz average75 x 0.45 = 33.8

Lab report average 85 x 0.30 = 25.5

Unknown 100 x 0.25 = 25.0

84.3 x 0.25 = 21.1 + 55.4 = 76.5 = C+

The following scale will be applied in determining your final letter grade.

  • A grade of “D” is a passing grade, but will not transfer.
  • Grades are rounded to the next letter grade (e.g. 92.5 is rounded to a 93 “A”; a 92.4 remains a 92 “A-“)

93 - 100%A

89 - 92A-

85 - 88B+

82 - 84B

78 - 81B-

74 - 77C+

70 – 73C

66 - 69C-

63 - 65D+

60 - 62D

0 –59F

BIOL 235 - STUDY TIPS:

Listen carefully in class and lab. You may record the class.

Ask questions when you don’t understand something

Take notes, and do so in an organized fashion. Make sure the information is organized with general headings and sub-headings. You may want to rewrite/reorganize the notes you take in class

Make sure you write down definitions correctly; it may help you to use flash cards.

Underline, circle, or highlight important points in your lecture notes, your textbook, and in your lab manual.

When reading the text, study the diagrams to help you visualize concepts.

Review the chapter summaries; they are there to help you.

Read over the lab exercise ahead of time.

Don't stay up all night cramming for an exam or quiz. Instead, organize your study time into small realistic blocks of study time through out the week.

To study for exams, review the material first using your notes, any handouts, and the text book. Then review any quizzes on the material to self-test yourself.

Use the on-line Companion Site Learning Center “Student Edition” at for on-line Flash cards, Animations, Self testing etc by chapter.

For every hour of class you should be studying at least 2 hours a week, therefore, you should be studying at least 10 hours a week for this course.

Fall 2011

BIOL 235 – Tentative Lecture Schedule

Each TUESDAY there will be either a quiz or an exam.

Quizzes will be administered towards the end of class.

A Brief Overviewcovered throughout the semester of Ch.#30-31:Microbial Interactions, Infection & Pathogenicity

Ch.#32-33: Nonspecific & Specific Immunity,andCh.#36 – Epidemiology & Public Health Microbiology

WEEK /

CHAPTER – TOPIC

1 / Aug 22-23 / Orientation; Ch.#1 – Evolution of Microorganisms and Microbiology
2 / Aug 29-30 / Ch.#1 – Evolution of Microorganisms and Microbiology Continued
Ch.#3 – Bacteria & Archaea Cell Structure & Function
3 / Sept 5-6 / Ch.#3 – Bacteria & Archaea Cell Structure & Function Continued
EXAM #1
4 / Sept 12-13 / Ch.#4 – Eukaryotic Cell Structure & Function
Ch.#6 – Microbial Nutrition
5 / Sept 19-20 / Ch.#6 – Microbial Nutrition Continued
Ch.#7 – Microbial Growth
6 / Sept 26-27 / Ch.#7 – Microbial Growth Continued
Ch.#9 – Introduction to Metabolism (Enzymes)
EXAM #2
7 / Oct 3-4 / Ch.#10 – Catabolism: Energy Release and Conservation (Respiration)(Fermentation)
October 10-11 / Fall Break
8 / Oct 17-18 / Ch.#11 – Anabolism: The Use of Energy & Biosynthesis (Photosynthesis)
Ch.#12 – Genes: Structure, Replication & Expression (Protein Synthesis)
9 / Oct 24-25 / Ch.#12 – Genes: Structure, Replication & Expression (Protein Synthesis) Continued
Ch.#14 – Microbial Genetics: Mechanisms of Genetic Variation (Mutations)
EXAM #3
10 / Oct 31-Nov 1 / Ch.#5 – Viruses and other Acellular Infectious Agents Structure & Function
Ch.#25 – The Viruses
Ch.#14 – Microbial Genetics: Mechanisms of Genetic Variation (Bacterial Genetics)
11 / Nov 7-8 / Ch.#14 – Microbial Genetics: Mechanisms of Genetic Variation (Bacterial Genetics) Continued
Ch.#15 – Recombinant DNA Technology
Ch.#18- The Archaea
Ch.#19-22 Bacteria
12 / Nov 14-15 / Ch.#19-22 Bacteria Continued
Ch.#23 – The Protists
EXAM #4
13 / Nov 21-22 / Ch.#23 – The Protists Continued
Ch.#24 – The Fungi
14 / Nov 28-29 / Ch.#8 – Control of Microorganisms in the Environment
EXAM #5
15 / Dec 6-9 / Ch.#34- Antimicrobial Chemotherapy
16 / TBA / Comprehensive Final Exam

Fall 2011

BIOL235 – Tentative Lab Schedule

Crawford Hall #257

01L Monday 4:00-5:50 02L Tuesday 3:00-4:50 03L Wednesday3:00-4:50

LAB OPEN

to check lab results after 24-48 hour incubation period

Tuesday 9:00 am – 1:50 pm

Wednesday 10:00 am – 2:50 pm

Friday 10:00 am – Noon

WEEKOF / REPORT DUE / QUIZ / PERFORMING
1 / Aug 22 / Orientation & Lab Safety
Ex.#1- Bright-field Microscope
2 / Aug 29 / Ex. 1 / Ex. 1 / Ex.#7 - Smear Preparation & Simple Staining
3 / Sept 5 / Ex. 7 / Ex. 7 / Ex.#8 - Gram Staining
4 / Sept 12 / Ex. 8 / Ex. 8 / Gram Stain Quiz Part A: Written Quiz
Part B: Interpretation of Gram Stained Slide
5 / Sept 19 / Ex.#9 - Acid-Fast Staining(Ziehl-Neelsen)
Ex.#10 – Endo-spore Staining (Schaeffer-Fulton)
6 / Sept 26 / Ex. 9-10 / Ex.9-10 / Ex.#16 - Streak-Plate Method& Differential Culture Media
7 / Oct 3 / Ex. 16 / Ex.16 / Ex.#20 - Carbohydrates Fermentation IEx.#22 – Carbohydrate Fermentation III
Ex.#23 - Lipids: Lipid Hydrolysis
8 / October 12
03L Only / Ex.20,22, 23 / Ex.20,22,23 / Ex.#42- Effects of Chemical Agents on Bacteria I: Disinfectants and other antimicrobial products
9 / Oct 17 / Ex.20,22, 23
03L Ex.42 / Ex.20,22,23
Ex.42 / Ex.#24 - Proteins, Amino Acids & Enzymes I: H2S & MotilityEx.#25 – Proteins, Amino Acids & Enzymes II: IMViC Tests
10 / Oct 24 / Ex. 24-25 / Ex.24-25 / Ex.#26 - Proteins, Amino Acids & Enzymes III- Casein HydrolysisEx.#28 – Proteins, Amino Acids & Enzymes V: Catalase Activity
10 / Oct 31 / Ex. 26 & 28 / Ex. 26 & 28 / Ex.#30 - Proteins, Amino Acids & Enzymes VII: Oxidase TestEx.#31 - Proteins, Amino Acids & Enzymes VIII: Urease ActivityEx.#34 - Proteins, Amino Acids & Enzymes XI: Nitrate Reduction
11 / Nov 7 / Ex. 30,31,34 / Ex. 30,31,34 / Ex.#43- Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)
12 / Nov 14 / Ex. 43 / Ex. 43 / Handout – Introduction to Parasitology
13 / Nov 21
01L & 02L Only / Parasitology / Parasitology / Ex.#42- Effects of Chemical Agents on Bacteria I: Disinfectants and other antimicrobial products
14 / Nov 28 / Ex. 42
03L Parasitiology / Ex. 42
Parasitiology / UNKNOWN
15 / Dec 5 / Unknown / Unknown Due; Clean-up; Review for Final Exam

SAFETY RULES IN THE MICROBIOLOGY LABORATORY

1.ONLY microbiology students are allowed in the laboratory.

2.NO EATING, DRINKING, GUM CHEWING, is permitted in the laboratory. Do not bring food or beverages into the lab. If this is not adhered to you will be dismissed from lab.

3.Do not remove cultures, reagents, or other materials from the laboratory at any time.

4.Lab coats are to be worn at all times in the laboratory. This helps to ensure that no culture material is accidentally deposited on your clothes or skin, and protects you and your clothes from spillage of chemicals and stains.

5.Jackets/coats are to be hung up on the hooks located on the back wall underneath the windows.

6.All other items, such as books, and bags, should be stored under your work area. Only those materials pertinent to your lab work, such as the lab manual and a notebook, should be brought to your laboratory workspace.

7.Note the location and use of the safetyeyewash station, safety shower, fire blanket, and first aid kit.

8.Minimize hand to face contact and avoid putting pencils or pens in the mouth.

9.Begin each laboratory session by disinfecting your work area. Saturate the area with disinfectant using a paper towel, and allow the area to dry. Repeat this procedure after you have finished your work to ensure that any material you have deposited on the work surface is properly disinfected.

10.Labeling is critical to avoid improper use or disposal of material. All culture material and chemicals are to be properly labeled with your name, laboratory section, date, and exercise.

11.Long hair must be secured in a ponytail. Be very careful with Bunsen burners. To avoid injuries, burners should be turned off when not in use. When reaching for objects, be careful not to place your hands into the flame.

12.Sterilize the inoculating loop and/or needle immediately BEFORE and AFTER each use and keep them in an upright position in the block holder provided.

13.Keep culture tubes in an upright position in the test tube rack at all times. Never lay a test tube flat on the workbench.

14.Contaminated material/glassware are not to be thrown in the trash cans. All contaminated material must be disposed of properly. Used swabs, pipettes, and disposable Petri dishes are placed in the red biohazard bag provided. Contaminated reusable glassware is to be placed on the dirty glassware cart located in the front of the room.

15.Clean up after yourself. Keep sink areas clean, and keep your microscope clean.

16.Wash your hands before leaving the lab.

17.In case of anyaccident or injury, report immediately to the laboratory instructor so that prompt and proper action can be taken. This includes burns, cuts and spills no matter how minor they are.

18.When “live” cultures are spilled notify the instructor immediately. Use paper towels to cover and contain the spread of the spill. Soak the towels with disinfectant. Allow 15 minutes for the disinfectant to react with the spilled culture. Use dry paper towels to pick up the wet towels and aseptically transfer the contaminated materials to the red biohazard bag provided.