BI432: IMMUNOLOGYWINTER, 2007
COURSE DESCRIPTION: Students are provided opportunities in lecture and in laboratory to learn the cellular and molecular events during a vertebrate immune response.
ASSESSMENT:Student learning is assessed by lecture and lab exams, and by c personal lab notebook.
LECTURE:10:00 - 10:50, MWF in NS122
TEXT:KUBY IMMUNOLOGY, 6th Ed. by Kindt, Goldsby, & Osborne, W.H. Freeman, 2007
INSTRUCTOR:Dr. Bob Turner, Office in NS222; Phone Ext. 88224 Office Hours Posted on web
LECTURE SCHEDULE
DATEDAYTOPICTEXT READING
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Jan.8MIntroduction to Course and to ImmunologyCh.1, pp 1-22
10 WCells and Organs of the Immune System ICh.2, pp 23-35
12FCells and Organs of the Immune System IICh.2, pp 35-51
Jan.15MMartin Luther King, Jr. DayNO CLASSES
17WInnate ImmunityCh.3, pp 52-71
19FAntigens and Antibodies ICh.4, pp 76-93
Jan.22MAntigens and Antibodies IICh.4, pp 93-110
24WImmunoglobulin Genes ICh.5, pp 111-127
26FImmunoglobulin Genes IICh.5, pp 128-144
Jan.29MAntigen-Antibody InteractionsCh.6, pp 145-167
31WThe Complement System Ch.7, pp 168-188
Feb2FMajor Histocompatability ComplexCh.8, pp 189-222
.5MT-Cell ReceptorCh.9, pp 223-244
7WEXAM I on material through Jan. 31
11FT-Cell Maturation, Activation & DifferentiationCh.10, pp 245-270
Feb.12MB-Cell Maturation, Activation & Differentiation ICh.11, pp 271-288
14WB-Cell Maturation, Activation & Differentiation IICh.11, pp 289-301
16FCytokinesCh.12, pp 302-326
Feb.19MLeukocyte Activation & MigrationCh.13, pp 327-350
21WCell-Mediated Cytotoxic ResponsesCh.14, pp 351-370
23FHypersensitivity ReactionsCh.15, pp 371-400
Feb.26MTolerance & AutoimmunityCh.16, pp 401-424
28WTransplantation ImmunologyCh.17, pp 425-446
March2FImmune Responses to Infectious DiseasesCh.18, pp 447-474
5MVaccinesCh.19, pp 475-492
7WEXAM II on material through Feb. 28212
9FAIDS & Other ImmunodeficienciesCh.20, pp 493-524
12MCancer & The Immune SystemCh.21, pp 525-545
14WExperimental SystemsCh.22, pp 546-574
16FReview for Final Exam
FINAL EXAM 10:00 A.M. to NOON, FRIDAY,MARCH 23 in NS122
POINT DISTRIBUTION
Exam 1 & 2 / 100 Points EachFinal Exam / 200
Quizzes / 100
Laboratory / 200
Total / 600
LABORATORY SCHEDULE
12:30 – 3:20 P.M. Thursday in NS204
DATE / LABORATORY TOPIC(S) / LABORATORY TECHNIQUES / READINGS in:Jan.16 / Immune System Organs & Cells:
Primary & Secondary Lymphoid Organs; Immune System Cells / Prepared Slides
Wright’s Stain of Human Blood
Use of Hemocytometer / Handout # 1
Jan.23 / Immune System Cells
Leukocyte Isolation and Actions:
Adhesion, Spreading & Phagocytosis / Separation of Peripheral Blood Components Fractionation of Spleen Cells Leukocyte Isolation from Blood: Actions of Leukocytes / Handout # 2
Jan.30 / Preparation of Tissue Antigens / Tissue Extraction, Dialysis & Protein Assay (Spreadsheets) / Batina, pp 37-42
Feb. 6 / Antibody Isolation / A: (NH4)2SO4 Precipitation
B: Affinity Chromatography
C: Column Chromatography / Batina, pp 153-155
Batina, pp 157-163
Hand-out #3
Feb.13 / Antigen-Antibody Interactions I / Immunoprecipitation Reactions:
Ouchterlony Double Diffusion / Batina, pp 75-86
Feb. 20 / Antigen-Antibody Interactions II / Immunoprecipitation Reactions:
Immunoprecipitation of Soluble Antigen / Handout -“Immunoprecipitation” CSH Portable Protocol #4
Feb. 27 / Analysis of Immunoprecipitated Antigen / SDS Gel Electrophoresis / Hand-out – “SDS Gel Electrophoretic Analysis of Proteins”
March 6 / Antibody-Antigen Interactions III / Western Blots / Handout -“Immunoblotting” CSH Portable Protocol #5
March 13 / Antibody-Antigen Interactions IV / ELISA Assay / Batina, pp 111-114
The content of the Immunology course will be drawn most heavily from the information in the figures and tables in the following list. The overview figures, indicated by the bold font, are to be considered particularly important because they summarize the essential information and will be used as points from which to initiate discussion of more detailed and specific content. After this information is presented in lecture, it will be applied to analysis of the appropriate Clinical Focus section from those provided in the text and also listed below.
Chapt / Figure #s / Table #s / Review Questions / Clinical Focus, p. #1 / 6, 10-13 / 3 / 20 Allergies & Asthma
2 / 2, 3, 6-9, 11, 14-20 / 4 / 32 Stem Cells
3 / 1,2,4-7, 12, 14 / 3,4 / 66 C-Reactive Protein
4 / 1, (2), 3-13, 17, 19-22, (24), 25 / 1-4 / 98 Passive Ab Therapy
5 / 1-6, 9-11,13, 14, 18-20, 24, 25 / 1-3 / 140 Genetic EngineerAb
6 / 1-16 / 1-3 / 163 Flow Cytometry-Leukemia
7 / 1, 5, 7, 10, 13, 15 / (1), 3 / 183 Complement Defect Disease
8 / 1-4 ,6, 7, 9, 10, 12, 16, 23, 24 / 2, 3 / 213 Ag Processing Disease
9 / 1, 2, 6, 8-10, 12, 13 / 1-3 / 232 TCR Changes Mark Cancer
10 / 2, 6, (5, 7-10), 11, 18 / 1, (2) / 266 Failed Apoptosis Disease
11 / 1-4, 6-12, 16, 18, 20, (22), 10 / 2, 4, 6 / 284 B-Cell Develop Disease
12 / 1, 2, 5-10, (12,13,16) / 4 / 322 Interferon Therapy
13 / 13, 14, (1, 4-8, 10) / 343 Leukocyte Adhesion Defect
14 / 1, 2, 4, 6, 7, 9, 10, 12, 15-17 / 364 MHC-KIR Gene Combination
15 / 1, 2, 5, 7, 8, 13, 14-16, 17, 18, 20 / 384 Genetics of Asthma
16 / 1, 4, (5-10) / 1, (3) / 416 Gender Diff in Autoimmune
17 / 1, (2-4), 6, 9, 10, 11, / 435 Clinical Xenotransplantation
18 / (1), 2, 4, 6, (5, 7), 8, 12, 13, (14) / 1, 3 / 468 Bioterrorism Agent Threat
19 / (2,3,5,6), 7 / 1-5 / 476 Vaccination Challenges
20 / 1, 4, (8), 9, 11, 12, 13, 16, / 2, (4,5), 6 / 507 Infant HIV Protection
21 / 1, 2, (3), 4-8, 10, 11, 13 / (1), 4 / 534 Vaccine Prevents Cancer
22 / 1-17 / 1, 4, (5,6) / 568 Diagnosis by Microarrays
BI432W07 Syllabus.DOC10/13/2018