Bhan, N., Xu, P., Khalidi, O. & Koffas, M. A. G. (2013) Redirecting Carbon Flux Into

Bhan, N., Xu, P., Khalidi, O. & Koffas, M. A. G. (2013) Redirecting carbon flux into malonyl-CoA to improve resveratrol titers: Proof of concept for genetic interventions predicted by OptForce computational framework. Chemical Engineering Science, 103, 109–114. doi:10.1016/j.ces.2012.10.009.

Xu, P., Gu, Q., Wang, W., Wong, L., Bower, A. G. W., Collins, C. H. & Koffas, M. A. G. (2013). Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nature Communications, 4 (1409), 1-8. doi:10.1038/ncomms2425.

Xu, P., Bhan, N.& Koffas, M. A. G. (2012). Engineering plant metabolism into microbes: from systems biology to synthetic biology. Current Opinion in Biotechnology, 24(2), doi:10.1016/j.copbio.2012.08.010.

Xu, P., Vansiri, A., Bhan, N. & Koffas, M. A. G. ePathBrick: A synthetic biology platform for engineering metabolic pathways in E. coli. ACS Synthetic Biology, In Press, doi:10.1021/sb300016b (2012).

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Malla, S., Koffas, M. A. G., Kazlauskas, R. J. & Kim, B.-G. Production of 7-O-Methyl Aromadendrin, a Medicinally Valuable Flavonoid, in Escherichia coli. Applied and Environmental Microbiology 78, 684-694, doi:10.1128/aem.06274-11 (2012).

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<p</p<hr />

Fowler, Z. L., Shah, K., Panepinto, J. C., Jacobs, A. & Koffas, M. A. G. Development of Non-Natural Flavanones as Antimicrobial Agents. Plos One 6, doi:10.1371/journal.pone.0025681 (2011).

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<p</p<hr />

Xu, P., Ranganathan, S., Fowler, Z. L., Maranas, C. D. & Koffas, M. A. G. Genome-scale metabolic network modeling results in minimal interventions that cooperatively force carbon flux towards malonyl-CoA. Metabolic Engineering 13, 578-587, doi:10.1016/j.ymben.2011.06.008 (2011).

<a href="papers/2011_Xu_et_al_Genome-scale_metabolic_network_modeling_results_in_minimal_interventions_that_cooperatively_force_carbon_ﬂux_towards_malonyl-CoA.pdf" target="_blank">Download</a>

<p</p<hr />

Santos, C. N. S., Koffas, M. & Stephanopoulos, G. Optimization of a heterologous pathway for the production of flavonoids from glucose. Metabolic Engineering 13, 392-400, doi:10.1016/j.ymben.2011.02.002 (2011).

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<p</p<hr />

Lim, C. G., Fowler, Z. L., Hueller, T., Schaffer, S. & Koffas, M. A. G. High-Yield Resveratrol Production in Engineered Escherichia coli. Applied and Environmental Microbiology 77, 3451-3460, doi:10.1128/aem.02186-10 (2011).

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Fowler, Z. L., Baron, C. M., Panepinto, J. C. & Koffas, M. A. G. Melanization of flavonoids by fungal and bacterial laccases. Yeast 28, 181-188, doi:10.1002/yea.1829 (2011).

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Xu, P., Ranganathan, S., Maranas, C. D., Koffas, M. & Ieee. An Integrated Computational and Experimental Study to Increase the Intra-cellular Malonyl-CoA: Application to Flavanone Synthesis. (2011).

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Lim, C.-G. & Koffas, M. A. G. Bioavailability and Recent Advances in the Bioactivity of Flavonoid and Stilbene Compounds. Current Organic Chemistry 14, 1727-1751 (2010).

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Xu, P &Koffas, M. A. G. Metabolic engineering of Escherichia coli for biofuel production. Biofuels, 1(3): 493-504, doi:10.4155/bfs.10.13(2010)

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Chemler, J. A., Lim, C. G., Daiss, J. L. & Koffas, M. A. G. A Versatile Microbial System for Biosynthesis of Novel Polyphenols with Altered Estrogen Receptor Binding Activity. Chemistry & Biology 17, 392-401, doi:10.1016/j.chembiol.2010.03.010 (2010).

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Chemler, J. A., Fowler, Z. L., McHugh, K. P. & Koffas, M. A. G. Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering. Metabolic Engineering 12, 96-104, doi:10.1016/j.ymben.2009.07.003 (2010).

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Bedekar, A., Shah, K. & Mattheos Koffas, Chapter 2 - Natural Products for Type II Diabetes Treatment, In: Allen I. Laskin; Sima Sariaslani; Geoffrey M. Gadd, Editor(s), Advances in Applied Microbiology, Academic Press, Vol. 71, pp. 21-73, ISSN 0065-2164, ISBN 9780123809933, doi:10.1016/S0065-2164(10)71002-9 (2010).

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Fowler, Z. & Koffas, M. Microbial Biosynthesis of Fine Chemicals: An Emerging Technology. In The Metabolic Pathway Engineering Handbook – Tools and Applications, CRC Press, pp. 22-1-30, ISBN 9781420077650 (2010).

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Fowler, Z. L., Gikandi, W. W. & Koffas, M. A. G. Increased Malonyl Coenzyme A Biosynthesis by Tuning the Escherichia coli Metabolic Network and Its Application to Flavanone Production. Applied and Environmental Microbiology 75, 5831-5839, doi:10.1128/aem.00270-09 (2009).

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Fowler, Z. L. & Koffas, M. A. G. Biosynthesis and biotechnological production of flavanones: current state and perspectives. Applied Microbiology and Biotechnology 83, 799-808, doi:10.1007/s00253-009-2039-z (2009).

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Koffas, M. A. G. Expanding the repertoire of biofuel alternatives through metabolic pathway evolution. Proceedings of the National Academy of Sciences of the United States of America 106, 965-966, doi:10.1073/pnas.0811893106 (2009).

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Chemler, J. A. & Koffas, M. A. G. Metabolic engineering for plant natural product biosynthesis in microbes. Current Opinion in Biotechnology 19, 597-605, doi:10.1016/j.copbio.2008.10.011 (2008).

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Koffas, M. Engineering flavonoid biosynthesis in microorganisms. Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology 150, S184-S184, doi:10.1016/j.cbpa.2008.04.497 (2008).

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Yan, Y., Li, Z. & Koffas, M. A. G. High-yield anthocyanin biosynthesis in engineered Escherichia coli. Biotechnology and Bioengineering 100, 126-140, doi:10.1002/bit.21721 (2008).

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<p</p<hr />

Leonard, E., Yan, Y., Fowler, Z. L., Li, Z., Lim, C.-G., Lim, K.-H. & Koffas, M. A. G. Strain improvement of recombinant Escherichia coli for efficient production of plant flavonoids. Molecular Pharmaceutics 5, 257-265, doi:10.1021/mp7001472 (2008).

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Leonard, E., Yan, Y., Chemler, J., Matern, U., Martens, S. & Koffas, M. A. G. Characterization of dihydroflavonol 4-reductases for recombinant plant pigment biosynthesis applications. Biocatalysis and Biotransformation 26, 243-251, doi:10.1080/10242420701685635 (2008).

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Chemler, J. A., Lock, L. T., Koffas, M. A. G. & Tzanakakis, E. S. Standardized biosynthesis of flavan-3-ols with effects on pancreatic beta-cell insulin secretion. Applied Microbiology and Biotechnology 77, 797-807, doi:10.1007/s00253-007-1227-y (2007).

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Leonard, E. & Koffas, M. A. G. Engineering of artificial plant cytochrome p450 enzymes for synthesis of isoflavones by Escherichia coli. Applied and Environmental Microbiology 73, 7246-7251, doi:10.1128/aem.01411-07 (2007).

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Leonard, E., Lim, K.-H., Saw, P.-N. & Koffas, M. A. G. Engineering central metabolic pathways for high-level flavonoid production in Escherichia coli. Applied and Environmental Microbiology 73, 3877-3886, doi:10.1128/aem.00200-07 (2007).

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Chemler, J. A., Yan, Y., Leonard, E. & Koffas, M. A. G. Combinatorial mutasynthesis of flavonoid analogues from acrylic acids in microorganisms. Organic Letters 9, 1855-1858, doi:10.1021/ol0703736 (2007).

<a href="papers/2007_Chemler_et_al_Combinatorial_mutasynthesis_of_flavonoid_analogues_from_acrylic_acids_in_microorganisms.pdf" target="_blank">Download</a>, <a href="papers/2007_Chemler_et_al_Combinatorial_mutasynthesis_of_flavonoid_analogues_from_acrylic_acids_in_microorganisms_Supporting_Information.pdf" target="_blank">Supporting Information</a>

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<h4>Earlier</h4>

Chemler, J. A., Yan, Y. & Koffas, M. A. G. Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae. Microbial Cell Factories 5, doi:10.1186/1475-2859-5-20 (2006).

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Leonard, E., Chemler, J., Lim, K. H. & Koffas, M. A. G. Expression of a soluble flavone synthase allows the biosynthesis of phytoestrogen derivatives in Escherichia coli. Applied Microbiology and Biotechnology 70, 85-91, doi:10.1007/s00253-005-0059-x (2006).

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<p</p<hr />

Leonard, E., Yan, Y. J. & Koffas, M. A. G. Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli. Metabolic Engineering 8, 172-181, doi:10.1016/j.ymben.2005.11.001 (2006).

<a href="papers/2006_Leonard_Yan_Koffas_Functional_expression_of_a_P450_flavonoid_hydroxylase_for_the_biosynthesis_of_plant_specific_hydroxylated_flavonols_in_Escherichia_coli.pdf" target="_blank">Download</a>

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Leonard, E., Yan, Y. J., Lim, K. H. & Koffas, M. A. G. Investigation of two distinct flavone synthases for plant-specific flavone biosynthesis in Saccharomyces cerevisiae. Applied and Environmental Microbiology 71, 8241-8248, doi:10.1128/aem.71.12.8241-8248.2005 (2005).

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<p</p<hr />

Yan, Y. J., Kohli, A. & Koffas, M. A. G. Biosynthesis of natural flavanones in Saccharomyces cerevisiae. Applied and Environmental Microbiology 71, 5610-5613, doi:10.1128/aem.71.9.5610-5613.2005 (2005).

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<p</p<hr />

Yan, Y. J., Chemler, J., Huang, L. X., Martens, S. & Koffas, M. A. G. Metabolic engineering of anthocyanin biosynthesis in Escherichia coli. Applied and Environmental Microbiology 71, 3617-3623, doi:10.1128/aem.71.7.3617-3623.2005 (2005).

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<p</p<hr />

Koffas, M. & Stephanopoulos, G. Strain improvement by metabolic engineering: lysine production as a case study for systems biology. Current Opinion in Biotechnology 16, 361-366, doi:10.1016/j.copbio.2005.04.010 (2005).

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Koffas, M. Evolutionary metabolic engineering. Metabolic Engineering 7, 1-3, doi:10.1016/j.ymben.2005.01.001 (2005).

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Koffas, M. A. G., Jung, G. Y. & Stephanopoulos, G. Engineering metabolism and product formation in Corynebacterium glutamicum by coordinated gene overexpression. Metabolic Engineering 5, 32-41, doi:10.1016/s1096-7176(03)00002-8 (2003).

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Koffas, M. A. G., Jung, G. Y., Aon, J. C. & Stephanopoulos, G. Effect of pyruvate carboxylase overexpression on the physiology of Corynebacterium glutamicum. Applied and Environmental Microbiology 68, 5422-5428, doi:10.1128/aem.68.11.5422-5428.2002 (2002).

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Koffas, M., Robervge, C., Lee, K. & Stephanopoulos, G. Metabolic engineering. Annual Review of Biomedical Engineering 1, 535-557, doi:10.1146/annurev.bioeng.1.1.535 (1999).

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<p</p<hr />

Koffas, M. A. G., Ramamoorthi, R., Pine, W. A., Sinskey, A. J. & Stephanopoulos, G. Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene. Applied Microbiology and Biotechnology 50, 346-352 (1998).

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<p</p<hr />

Bhan, N., Xu, P., Khalidi, O. &amp; Koffas, M. A. G. (2013) Redirecting Carbon Flux Into

Bhan, N., Xu, P., Khalidi, O. & Koffas, M. A. G. (2013) Redirecting Carbon Flux Into