(B)The Diagram Shows a Cell During the First Division of Meiosis

1.(a)When a cell divides, the genetic material can divide by mitosis, by meiosis or by neither of these processes. Complete the table with a tick to show the process by which you would expect the genetic material to divide in each of these examples.

mitosis / meiosis / neither
The division of plasmids in bacterial reproduction.
The stage in the formation of male gametes in a plant in which haploid daughter cells are formed from a haploid parent cell.
Cell division which takes place in the growth of a human testis between birth and five years of age.
The stage in the lifecycle of a protoctistan in which a large number of genetically different spores are produced.

(2)

(b)The diagram shows a cell during the first division of meiosis.

Complete the diagram below to show the appearance of the chromosomes in each of the four daughter cells formed at the end of the second division of meiosis.

(2)

(c)In an insect, 16 chromatids were visible in a cell at the start of the first division of meiosis. How many chromosomes would there be in a normal body cell from this insect?

......

(1)

(Total 5 marks

2.The diagram shows stages of meiosis in a human testis. Each circle represents a cell.

(a)In each empty circle, write the number of chromosomes that would be found in the cell.

(1)

(b)Describe two ways in which meiosis contributes to genetic variation.

1......

......

2......

......

(2)

(c)Explain the importance of genetic variation in the process of evolution.

......

(2)

(Total 5 marks)

3.A queen honey bee can lay both fertilised and unfertilised eggs. Fertilised eggs develop into diploid females and unfertilised eggs develop into haploid males. The diagram shows the formation of gametes in female bees and in male bees.

Giving a reason for your answer in each case, name the type of cell division in the bee that produces:

(i)female gametes;

......

(1)

(ii)male gametes.

......

(1)

(b)The table shows some features which contribute to variation in the offspring of bees. Complete the table with a tick if the feature may contribute or a cross if it does not.

Feature / Female offspring / Male offspring
Crossing over
Independent segregation of chromosomes
Random fusion of gametes

(2)

(c)Body colour in bees is determined by a single gene. The allele B for yellow body is dominant to the allele b for black body. Explain why, in the offspring of a mating between a pure-breeding black female and a yellow male, all the males will be black.

......

(1)

(Total 5 marks)

4.The diagram below shows the length of cobs in two pure breeding varieties of maize plant and the F1and F2 generations derived from a cross between them.

Parental Generation

(a)What information does the term pure breeding give about genotype?

......

(1)

(b)Give the modal class of the F1 generation.

......

(1)

(c)(i)Explain what is meant by polygenic inheritance.

......

(1)

(ii)Explain the evidence from the diagram below which suggests that inheritance of cob length is polygenic.

......

...... (2)

(d)What is the evidence that differences in cob length in the parental generation are partly due to:

(i)genetic difference;

......

(1)

(ii)environmental differences?

......

(1)

In maize a single gene with two alleles controls the type of carbohydrate stored in the cells of the plant. Starchy varieties of maize have starch grains which stain blue-black with iodine solution; waxy varieties have starch grains which stain red. The allele for starch, W, is dominant to that for waxy, w.

(e)Explain what is meant by:

(i)a gene;

......

(1)

(ii)an allele.

......

(1)

(f)Pollen from a single maize plant was dusted on a microscope slide and stained with iodine solution. The results are shown in the table.

Pollen grains stained blue-black with iodine solution / Pollen grains stained red with iodine solution
58 / 64

What is the genotype of:

(i)the pollen grains stained red with iodine solution;

......

(1)

(ii)the parent plant from which these pollen grains were taken?

......

(1)

(g)In a field of maize the frequency of allele W was 0.7 and the frequency of allele w was 0.3.

(i)If the maize plants were randomly fertilised, what frequencies of these two alleles would be expected in the next generation?

......

(1)

(ii)Use the Hardy Weinberg equation to calculate the percentage of heterozygous plants in the field of maize.

Answer:...... (3)

(Total 15 marks)

5.In cats, one of the genes for coat colour is present only on the X chromosome. This gene has two alleles. The allele for ginger fur, XB, is dominant to that for black fur, Xb

(a)All the cells in the body of a female mammal carry two X chromosomes. During an early stage of development one of these becomes inactive and is not expressed. Therefore female mammals have patches of cells with one X chromosome expressed and patches of cells with the other X chromosome expressed. Tortoiseshell cats have coats with patches of ginger and patches of black fur.

(i)What is the genotype of a tortoiseshell cat?

......

(1)

(ii)Explain why there are no male tortoiseshell cats.

......

(1)

(b)A cat breeder who wished to produce tortoiseshell cats crossed a black female cat with a ginger male. Complete the genetic diagram and predict the percentage of tortoiseshell kittens expected from this cross.

Parental phenotypes: black femaleginger male

Parental genotypes:......

Gamete genotypes:......

Offspring genotypes: ......

Percentage of tortoiseshell kittens: ......

(3)

(Total 5 marks)

6.The formation of sperm cells involves mitosis and meiosis. The graphs show the amount of DNA in the nuclei of cells taken from different parts of a mammalian testis.

(a)(i)Explain the evidence which shows that Graph 1 represents cells undergoing mitosis.

......

(1)

(ii)Explain how the events of meiosis result in three groups of nuclei in Graph 2.

......

(3)

(b)Explain one way in which meiosis can produce daughter cells each with different DNA.

......

(2)

The flow chart shows one method of finding the sequence of bases in a small piece of DNA. A large number of identical pieces of DNA are produced and treated in the same way.

Only enough chemical is added to destroy one of the bases concerned. Destroying a base results in the strand breaking into two separate pieces. In treatment 1, for example, the strand of DNA shown below

will give one of two different radioactively labelled pieces.
Either

(c)(i)A nucleotide is made up of three components joined by condensation reactions. Which component would be labelled with 32P?

...... (1)

(ii)Name the type of chemical bond which is broken when the two strands in a DNA molecule are separated.

...... (1)

The samples of treated DNA are analysed by electrophoresis. The results in the diagram show the position of the radioactive fragments.

(d)How many times in the strand of DNA being investigated does:

(i)the base cytosine occur;

......

(1)

(ii)the base thymine occur?

......

(1)

(e)Which of the radioactive fragments labelled 1 to 14 in the diagram only contains one base?
Explain how you arrived at your answer.

Fragment number ......

Explanation ......

......

(3)

(Total 13 marks)

7.(a)Explain the meaning of the term gene pool.

......

(1)

In two-spot ladybirds, the colour of the wing-cases is genetically determined. The allele for red wing-cases, R, is dominant to that for black wing-cases, r.

(b)In a ladybird with black wing-cases, how many copies of the r allele would you expect to find in a nucleus taken from a muscle cell in the insect's leg? Explain your answer.

......

(1)

(c)In a population of two-spot ladybirds found on a patch of nettles, 168 had red wing-cases and 32 had black wing-cases. Showing your working in each case, calculate the frequency of:

(i)the r allele;

Answer: ......

(2)

(ii)the R allele.

Answer: ......

(1)

(Total 5 marks)

8.(a)The inheritance of the ability to produce hydrogen cyanide is controlled by two genes which are located on different chromosomes. The dominant allele of one gene, G, controls the production of enzyme G which converts a precursor to linamarin. The dominant allele of the other gene, E, controls the production of enzyme E which converts linamarin to hydrogen cyanide. This is summarised in the diagram.

(i)Explain why plants homozygous for the allele g will not produce hydrogen cyanide when their tissues are damaged.

(2)

(ii)Plants with genotypes GgEe and GgEE were crossed. Use a genetic diagram to predict the percentage of plants produced from this cross which would release hydrogen cyanide when their tissues were damaged.

(4)

Recently a strain of genetically engineered clover has been developed which has a high concentration of proteins rich in sulphur-containing amino-acids. A piece of DNA was prepared which contained the three different genes. This was inserted into a clover plant.

(b)The copy of Gene 1 used in this experiment was obtained from the mRNA of the sunflower seeds.

(i)Explain how enzymes could be used to obtain the gene from the mRNA.

(3)

(ii)Explain why it would be an advantage to obtain the gene from the mRNA rather than from the DNA of the sunflower seeds.

(2)

(Total 11 marks)

1.(a)

mitosis / meiosis / neither

All four answers correct, 2 marks

(2)

At least two answers correct 1 mark

(b)Four haploid cells, each with two chromosomes;
Correct combinations of chromosomes;

(2)

(1)

(Total 5 marks)

2.(a)Meiosis I

2323

meiosis II

23232323;1

(b)Random assortment of chromosomes/correct description;
crossing over/chiasma formation/correct description;
by reducing chromosome number it makes possible random fertilisation;MAX. 2

(c)Causes variation in phenotype/characteristics of organisms/some better
adapted/have more favourable characteristics;
correct reference to (natural) selection of better adapted organisms;
selection of different phenotypes in different environments;
eventually leads to species change/change in gene pool/change in gene
frequencies;MAX. 2

(Total 5 marks)

3.(a)(i)Meiosis as number of chromosomes halved;

(1)

(ii)Mitosis as number of chromosomes stays the same;

(1)

(b)

Feature / Female offspring / Male offspring
Crossing over / /
Independent segregation of chromosomes / /
Random fusion of gametes / / x

(2)

(c)Offspring will contain a single b allele only;

(1)

(Total 5 marks)

4.(a)Homozygous (for alleles concerned);

(1)

(b)24 – 26cm;

(1)

(c)(i)Single characteristic controlled by many genes/more than one gene;

(1)

(ii)Many lengths/wide range of lengths/phenotypes;
in F2 generation;

(2)

(d)(i)Two different groups/produce intermediate offspring;

(1)

(ii)Each parent shows variation although genotype the same;

(1)

(e)(i)A piece of DNA which codes for a single protein/polypeptide;

(1)

(ii)A particular form of a gene;

(1)

(f)(i)w;

(1)

(ii)Ww;

(1)

(g)(i)The same/0.7 and 0.3;

(1)

(ii)Hardy-Weinberg equation given correctly as p2 + 2pq + q2 (= 1);
understands p = 0.7 and q = 0.3;
percentage of heterozygotes = 42

(3)

(Total 15 marks)

5.(a)(i)XBXb /XBXb

(1)

(ii)Need two X chromosomes / males have only one X / male has a Y / X and Y
chromosome / no allele on Y;

(1)

(b)Parental genotypesXbXb XBY
gamete genotypes Xb XB Y;
offspring genotypes XbXB XbY / correct from
given gametes
if sex-linked;
percentage of tortoiseshell kittens = 50%/ correct from offspring genotype
given;

(3)

(Total 5 marks)

6.(a)(i)DNA will be halved / less /DNA doubles /increases / there are 2 groups of
nuclei;

(1)

(ii)Group with most DNA is before division;
in first division amount of DNA halved;
second division halves this again;
three different amounts of DNA / quoted figures;

(3)

(b)Random segregation /independent assortment;
allows different combinations DNA /chromosomes;

crossing over /chiasmata;
allows mixing of / swapping / recombination of DNA / mixing alleles / genes
/ exchange of parts of chromatids / chromosomes;

(2)

(c)(i)Phosphate / phosphoric acid / P ;

(1)

(ii)Hydrogen (bond);

(1)

(d)(i)5;

(1)

(ii)3;

(1)

(e)Fragment 14 / 13;
single base represents smallest / lightest piece of DNA;
smallest piece moves furthest / fastest;

(3)

(Total 13 marks)

7.(a)(All) the alleles/genes in a population/ definition;

(b)Two. All somatic/ body cells are diploid/have two copies of each
chromosome/ must be homozygous for phenotype to be expressed;

(c)(i)2 marks0.4 / 40%
1 markincorrect figure obtained from 0.162

(ii)60% / 0.6 but accept answer derived correctly from (c)(i);1

(Total 5 marks)

8.(a)(i)Do not produce enzyme G/produce non-functional enzyme G;
No linamarin formed;

(2)

(ii)(Parental genotypes: GgEe GgEE)

Gametes: GE Ge gE geGE gE;

GE / Ge / gE / ge
GE / GGEE / GGEe / GgEE / GgEe
gE / GgEE / GgEe / ggEE / ggEe

75%producing cyanide;

Set out in logical genetic diagram / with gamete genotypes labelled;

(4)

(b)(i)Reverse transcriptase;
makes single strand of DNA/cDNA from RNA;
Double strand then formed;
DNA polymerase;

(MAX 3)

(ii)If from DNA all genes are present in cell;
mRNA from activated genes only/codes for one protein;
DNA has introns/non-coding /junk DNA;
Have been edited out in mRNA;

(Max 2)

(Total 11 Marks)