Autopolyploidy Leads to Rapid Genomic Changes in Arabidopsis Thaliana

Electric Supplementary Materials

Title:

Autopolyploidy leads to rapid genomic changes in Arabidopsis thaliana

Authors:

Shihong Liu1, Yan Yang1, 2, Fang Wei1*, Jifa Duan1, Janeen Braynen1, Baoming Tian1, 2*, Gangqiang Cao1, Gongyao Shi1, Jiachen Yuan1

Affiliation:

1. School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R.China

2. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang Henan 455000, P.R.China

* Corresponding authors: Fang Wei, E-mail address: , and Baoming Tian, E-mail address: .

Acknowledgments: This work was financially supported by the National Natural Science Foundation of China (No. 31600995), and Education Department of Henan Province of China (13A180437).

Figs. S1, S2 and S3

Table S1 and S2

Figure. S1 Schematic of generation breeding of Arabidopsis diploid, synthesis of autotetraploid and triploid.

Figure. S2 Identification of autotriploids. (A) The leaf, silique, and flower of Arabidopsis of three different ploidy plants (2x, 3x and 4x). (B) Flow cytometry of Arabidopsis polyploids. Leaves that have been growing for 5 to 6 weeks were used to identify the triploid by flow cytometry. Note the position of the peaks of the polyploids.

Figure. S3 AFLP banding profiles. Autotetraploid of three ecotypes (Col, Ler and Ws) in A. thaliana were compared with their own diploid genotypes. Blue arrows represent bands present in diploid but absent in autotetraploid；red arrows represent bands absent in autotetraploid but present in diploid.

Table S1. Adapters and primers of AFLP technique

No. / Sequences（5‘—3‘）
EcoR I
Mse I
Pre-amplification primers
Selective amplification primers / Adapter1
Adapter2
Adapter1
Adapter2
EcoR I
Mse I
E-AAC
E-AAT
E-AAG
M-CAG
M-CAT
M-CTT
M-CTA
M-CAA / CTCGTAGACTGCGTACC
AATTGGTACGCAGTCTAC
GACGATGAGTCCTGA
TACTCAGGACTCAT
GACTGCGTACCAATTCA
GATGAGTCCTGAGTAAC
GACTGCGTACCAATTCAAC
GACTGCGTACCAATTCAAT
GACTGCGTACCAATTCAAG
GATGAGTCCTGAGTAACAG
GATGAGTCCTGAGTA ACAT
GATGAGTCCTGAGTAACTT
GATGAGTCCTGAGTAACTA
GATGAGTCCTGAGTAACAA

Table S2. Primers of semi-quantitative PCR

Gene / Forward sequence（5‘—3‘） / Reverse sequence（5‘—3‘）
AT3G48770
AT1G12580
AT5G20420
TBL7
AT3G04470
AT2G19260
WEE1
XTH33
AT2G41890
AT3G04170
PMR6
BT1
AT3G56150
AT2G46980
AT4G04370
β-actin / GGATGTATGGTTCGGGTTCT
GGATGGGTTTGGTTGTGAAG
CGCCAATCCGTATGTTTACC
GTGCTTATCATCGCTGGAAC
GCTCGCCATTCCCATTATTC
ATGATCCACAAAGTTCCCGA
TCTCTGCTTCAATTCGGTCA
TATTCAGGTCAATGGCTCCC
GTGTTCTTCTTGGCGTTTCA
TTGTCTCTTGTTACGACCCAT
ACGAGGTTATGTTGTTGGGT
CACCTGACCTCTGTCTCAAG
GTGGTTAAGCCTGCTAAGGA
TAACAGAAGCGGGAAAGACA
AAAGTGTTCGAGGAAATGCG
CGTGAGAAGAACAGGGTGCT / CCACACGGTTCTCATGTTTC
GGTGAAGGCAGTAACCTCTT
CAATCTTCTCAGCTTGCCAC
TTCCAACCCTGTCATCAACA
GGCTACATGATTTGAACGGG
CCCATCACCCCTTATCGTAG
TTCCTGTGAAACTCTCCTGG
CCCAAACGGTGACGTATAGA
ACGAAGCAAAGCAGAGGATA
GACTAGGAGCTTTCCCTTGA
GTTTCGAGTGAGCTGGTCTA
CGGCAAGATTCAGATTGGTC
TAGGTTCATCCACATCGGAC
TTTCCTCTCGCAGACTTGTT
GATGCTCCAAATGTCTGCTG
TGGTGTCATGGTTGGGATGG