Assessing the Variability of Bacterial Composition in Background Aerosols of the Eastern

Aerobiologia

Assessing the variability of bacterial composition in background aerosols of the Eastern Mediterraneanduring a rapid change of meteorological conditions

P.N. Polymenakou (corresponding) · M. Mandalakis

Hellenic Centre for Marine Research, Institute of Marine Biology and Genetics, Gournes Pediados, P.O.Box 2214, GR71003, Heraklion, Crete, Greece

e-mail:

M. Mandalakis

University of Crete, Department of Chemistry, Vasilika Vouton, GR 71500, Heraklion, Crete, Greece

e-mail:

Table S1.

Summary of meteorological conditions of the six aerosol samples analyzed for microbial assemblage composition

Sample / Sampling Period / Sampling Duration / TSP (μg m-3) / Temperature (oC) / Humidity (%) / Wind Speed (km h-1) / Wind Direction
F44 / 1 / May 16, 2008 12:00 - 18:00 / 31 / 21.0 ± 0.1 / 73 ± 5 / 17.8 ± 2.6 / North
F45 / 1 / May 16, 2008 18:00 - 00:00 / 24 / 18.4 ± 2.3 / 79 ± 4 / 6.8 ± 5.2 / North
F46 / 1 / May 17, 2008 00:00 - 06:00 / 30 / 14.1 ± 0.8 / 72 ± 4 / 11.7 ± 3.4 / North
F47 / 1 / May 17, 2008 06:00 - 12:00 / 24 / 19.7 ± 3.4 / 58 ± 8 / 8.0 ± 5.5 / North
F56 / 2 / May 19, 2008 12:00 - 18:00 / 57 / 31.1 ± 0.9 / 26 ± 3 / 38.3 ± 4.0 / South
F57 / 2 / May 19, 2008 18:00 - 00:00 / 56 / 27.6 ± 0.7 / 32 ± 5 / 24.1 ± 8.1 / South

Table S2. Summary of results from the BIOENV analysis of similarity matrices based on bacterial assemblage composition (including plant-related sequences). For the variable “wind direction” the values of 0 and 180 for the North and South winds, respectively, was used in the input file.

Bacterial assemblage composition including plant-related sequences
BIOENV analysis
Variable 1 / TSP
Variable 2 / Humidity
Variable 3 / Wind Direction
Highest rank correlation / 0.642

Table S3.

Clones identified from aerial samples. The closest match (in NCBI sequence database) is presented.

Accession No. / Clone / BLAST match (with accession No.) / Origin (Study) / Match (%)
JQ512845 / >F44_11E / Uncultured bacterium ncd1030b03c1 (HM339935) / human skin microbiome (Kong et al. unpublished) / 99
Propionibacterium acnes 6609 (CP002815) / pathogenic human (Hunyadkurti et al. 2011) / 99
JQ512846 / >F44_12A / Uncultured bacterium nbw1039e10c1 (GQ055855) / human skin microbiome (Grice et al. 2009) / 99
JQ512847 / >F44_12B / Uncultured bacterium VA25L-87 (DQ639142) / human (Lu et al. unpublished) / 99
JQ512848 / >F44_12C / Uncultured bacterium VA25L-87 (DQ639142) / human (Lu et al. unpublished) / 99
JQ512849 / >F44_11H / Uncultured bacterium ncd436f09c1 (HM324998) / human skin microbiome (Kong et al. unpublished) / 98
JQ512850 / >F45_1H / Uncultured bacterium ncd382h10c1 (HM313725) / human skin microbiome (Kong et al. unpublished) / 97
JQ512851 / >F45_2A / Uncultured ELU0112-T277-S-NI_000127 (HQ788131) / human (Li et al. unpublished) / 99
JQ512852 / >F45_2F / Uncultured Pseudomonas sp. clone YJQ-50 (AY569299) / hot spring (Peng et al. unpublished) / 99
JQ512853 / >F45_2G / Uncultured bacterium ncd1467a07c1 (JF126346) / human skin microbiome (Kong et al. unpublished) / 98
JQ512854 / >F45_5D / Uncultured bacterium ncd1052e05c1 (HM343917) / human skin microbiome (Kong et al. unpublished) / 99
JQ512855 / >F45_6C / Uncultured bacterium OK2_d10_3 (EU777074) / mammals gut microbes (Ley et al. 2008) / 99
JQ512856 / >F45_6G / Lactobacillus algidus M 6 A9 (NR028617) / food (Kato et al. 2000) / 99
JQ512857 / >F45_7A / Uncultured ELU0166-T394-S-NIPCRAMgANa_000605 (HQ811620) / human (Li et al. unpublished) / 99
JQ512858 / >F45_7D / Uncultured bacterium ncd1527c04c1 (JF133340) / human skin microbiome (Kong et al. unpublished) / 99
JQ512859 / >F45_9A / Uncultured bacterium ncd2411f06c1 (JF209679) / human skin microbiome (Kong et al. unpublished) / 99
JQ512860 / >F45_10B / Uncultured Haemophilus sp. 7BB565 (FJ976274) / human (Bik et al. 2010) / 99
JQ512861 / >F45_11H / Uncultured ELU0042-T229-S-NIPCRAMgANa_000227 (HQ758481) / human (Li et al. unpublished) / 99
JQ512862 / >F45_12C / Uncultured bacterium ncd2762a10c1 (JF237886) / human skin microbiome (Kong et al. unpublished) / 99
JQ512863 / >F45_12E / Pseudomonas sp. CK5 (JN695712) / soil (Joshi et al. unpublished) / 99
JQ512864 / >F46_1D / Uncultured Treponema sp. 24B743 (FJ976410) / human (Bik et al. 2010) / 98
JQ512865 / >F46_1G / Uncultured bacterium Oh_3127A1C (EU137565) / fleas (Jones et al. 2008) / 99
JQ512866 / >F46_1H / Uncultured bacterium ncd1039f12c1 (HM343071) / human skin microbiome (Kong et al. unpublished) / 97
JQ512867 / >F46_3B / Uncultured bacterium NarTC10 (GQ401691) / soil (Unlik et al. unpublished) / 98
JQ512868 / >F46_3E / Uncultured bacterium VA25L-87 (DQ639142) / human (Lu et al. unpublished) / 99
JQ512869 / >F46_3H / Uncultured ELU0166-T394-S-NIPCRAMgANa_000239 (HQ811254) / human (Li et al. unpublished) / 99
JQ512870 / >F46_4C / Uncultured bacterium ncd1785c04c1 (JF154739) / human skin microbiome (Kong et al. unpublished) / 99
JQ512871 / >F46_4E / Uncultured bacterium OK2_d10_3 (EU777074) / mammals gut microbes (Ley et al. 2008) / 98
JQ512872 / >F46_4H / Uncultured ELU0166-T415-S-NIPCRAMgANa_000275 (HQ812623) / human (Li et al. unpublished) / 99
JQ512873 / >F46_5F / Uncultured bacterium VA25L-87 (DQ639142) / human (Lu et al. unpublished) / 98
JQ512874 / >F46_6E / Uncultured bacterium ncd155d03c1 (HM260490) / human skin microbiome (Kong et al. unpublished) / 99
JQ512875 / >F46_6H / Azotobacter beijerinckii ICMP 8673 (EF100152) / soil (Young and Park, unpublished) / 99
JQ512876 / >F46_7H / Uncultured bacterium ncd2798C04c1 (JF223787) / human skin microbiome (Kong et al. unpublished) / 100
JQ512877 / >F46_8F / Methylovorus menthalis MM (HQ380796) / plants (Doronina et al. 2011) / 99
JQ512878 / >F46_8H / Uncultured bacterium ncd1259d04c1 (JF114643) / human skin microbiome (Kong et al. unpublished) / 99
JQ512879 / >F46_9B / Bacillus sp. GN-2 (EU305732) / sodium chloride resistant (Guo and Li, unpublished) / 99
JQ512880 / >F46_10C / Uncultured bacterium ncd2043e01c1 (JF176335) / human skin microbiome (Kong et al. unpublished) / 99
JQ512881 / >F46_11D / Pseudomonas sp. Nl2 (GU391519) / plant (Jin et al. unpublished) / 99
JQ512882 / >F46_12A / Uncultured ELU0169-T415-S-NIPCRAMgANa_000442 (HQ812790) / human (Li et al. unpublished) / 99
JQ512883 / >F46_12B / Methylovorus menthalis MM (HQ380796) / plants (Doronina et al. 2011) / 99
JQ512884 / >F46_12E / Uncultured bacterium 27_156 (EU770638) / cave (Bastian and Alabouvette, unpublished) / 98
JQ512885 / >F46_12F / Uncultured Streptococcus sp. 202H12(oral) (AM420055) / human (Bolivar et al. unpublished) / 99
JQ512886 / >F47_6F / Uncultured bacterium BS35 (EU358710) / biogas digester (Liu et al. 2009) / 99
JQ512887 / >F47_7E / Uncultured bacterium ncd17920d10c1 (JF142809) / human skin microbiome (Kong et al. unpublished) / 99
JQ512888 / >F47_8D / Uncultured bacterium ncd2535e08c1 (JF203489) / human skin microbiome (Kong et al. unpublished) / 97
JQ512889 / >F47_12B / Uncultured beta proteobacterium H70 (FM958427) / flies (Moret et al. unpublished) / 99
JQ512890 / >F47_1D / Uncultured bacterium ncd439b03c1 (HM325162) / human skin microbiome (Kong et al. unpublished) / 99
JQ512891 / >F47_1G / Salinicoccus kunmingensis YIM Y15 (NR_043979) / salt mine (Chen et al. 2007) / 96
JQ512892 / >F47_1H / Uncultured bacterium ncd2798C04c1 (JF223787) / human skin microbiome (Kong et al. unpublished) / 100
JQ512893 / >F47_2C / Alteromonas sp. YSN412 (JN622161) / marine (Jiao et al. unpublished) / 99
JQ512894 / >F47_3B / Leuconostoc mesenteroides MFPC40A28-01 (JF756320) / food (Lucquin et al. 2012) / 99
JQ512895 / >F47_3G / Uncultured bacterium OK2_d10_3 (EU777074) / mammals gut microbes (Ley et al. 2008) / 99
JQ512896 / >F47_4C / Alkalibacterium iburiense JCM 12662 (AB294177) / marine (Ishikawa et al. 2009) / 99
JQ512897 / >F47_5B / Uncultured ELU0157-T387-S-NIPCRAMgANb_000196 (HQ806344) / human (Li et al. unpublished) / 99
JQ512898 / >F47_5F / Uncultured bacterium ncd1466a11c1 (JF126281) / human skin microbiome (Kong et al. unpublished) / 99
JQ512899 / >F47_7G / Uncultured bacterium ncd2075g10c1 (JF178463) / human skin microbiome (Kong et al. unpublished) / 99
JQ512900 / >F47_9B / Uncultured bacterium BARB_aaa04a02 (EU475578) / mammals gut microbes (Ley et al. 2008) / 99
JQ512901 / >F47_9G / Uncultured bacterium ncd1038c07c1 (HM342994) / human skin microbiome (Kong et al. unpublished) / 99
JQ512902 / >F47_10B / Uncultured Porphyromonas sp. 25B986 (FJ976429) / human (Bik et al. 2010) / 99
JQ512903 / >F47_11H / Uncultured ELU0170-T416-S-NIPCRAMgANa_000555 (HQ813500) / human (Li et al. unpublished) / 99
JQ512904 / >F47_12C / Uncultured ELU0169-T415-S-NIPCRAMgANa_000493 (HQ812841) / human (Li et al. unpublished) / 99
JQ512905 / >F47_12G / Pseudomonas stutzeri LYS-86 (GQ402828) / activated sludge (Sheng and Hong unpublished) / 99
JQ512906 / >F56_1E / Uncultured Veillonella sp. clone EHFS1_S01e (EU071469) / clean room (Nellen et al. unpublished) / 99
JQ512907 / >F56_1G / Uncultured bacterium ncd2461d03c1 (JF212978) / human skin microbiome (Kong et al. unpublished) / 99
JQ512908 / >F56_2E / Uncultured bacterium FMFRc10_468 (EU531959) / gut microbiota (Khachatryan et al. 2008) / 99
JQ512909 / >F56_2F / Uncultured bacterium MA03E11 (FM873723) / house dust (Taubel et al. 2009) / 99
JQ512910 / >F56_3E / Uncultured ELU0030-T148-S-NIPCRAMgANa_000127 (HQ750935) / human (Li et al. unpublished) / 99
JQ512911 / >F56_3H / Prevotella melaninogenica SEQ236 (JN867309) / pathogenic human (Wybo et al. unpublished) / 99
JQ512912 / >F56_4C / Uncultured bacterium ncd1466a11c1 (JF126281) / human skin microbiome (Kong et al. unpublished) / 99
JQ512913 / >F56_4D / Uncultured bacterium MB-A2-118 (AY093466) / marine sediment (Reed et al. 2002) / 99
JQ512914 / >F56_4E / Uncultured bacterium ncd2815f04c1 (JF241296) / human skin microbiome (Kong et al. unpublished) / 99
JQ512915 / >F56_4F / Uncultured bacterium MA03F08 (FM873726) / house dust (Taubel et al. 2009) / 99
JQ512916 / >F56_5A / Abiotrophia para-adiacens strain TKT1 (AB022027) / pathogenic human (Kanamoto et al. 2000) / 99
JQ512917 / >F56_5D / Uncultured ELU0144-T169-S-NIPCRAMgANa_000433 (HQ799750) / human (Li et al. unpublished) / 99
JQ512918 / >F56_6E / Uncultured bacterium RESET_121H11 (JN874090) / marine hydrothermal plume (Sylvan et al. unpublished) / 99
JQ512919 / >F56_8C / Leuconostoc sp. G1 (EF204328) / food (Hantsis-Zacharov and Halpern, 2007) / 99
JQ512920 / >F56_9E / Pseudomonas stutzeri LYS-86 (GQ402828) / activated sludge (Sheng and Hong, unpublished) / 99
JQ512921 / >F56_9H / Acinetobacter marinus (AY633607) / sea water (Yoon et al. 2007) / 96
JQ512922 / >F56_10C / Uncultured bacterium ncd1268f04c1 (JF072413) / human skin microbiome (Kong et al. unpublished) / 99
JQ512923 / >F56_10G / Uncultured delta proteobacterium COREB41 (EF562226) / water (Sahl et al. 2008) / 100
JQ512924 / >F56_10H / Uncultured bacterium ncd109f01c1 (HM257669) / human skin microbiome (Kong et al. unpublished) / 99
JQ512925 / >F56_11F / Bacillus sp. TSWCSN6 (AM888180) / soil (Bhattacharya and Chakrabarti, unpublished) / 98
JQ512926 / >F56_12A / Uncultured bacterium ncd2185c09c1 (JF190565) / human skin microbiome (Kong et al. unpublished) / 100
JQ512927 / >F56_12E / Uncultured bacterium isolate BF0002A038 (AM696583) / indoor air (Rintala et al. 2008) / 99
JQ512928 / >F57_4A / Uncultured bacterium ncd1101d08c1 (HM337538) / human skin microbiome (Kong et al. unpublished) / 99
JQ512929 / >F57_5A / Uncultured bacterium ODP1251B22.36 (AB177327) / sediment (Inagaki et al. 2006) / 99
JQ512930 / >F57_7A / Uncultured bacterium FD03C07 (FM873417) / house dust (Taubel et al. 2009) / 99
JQ512931 / >F57_9A / Uncultured bacterium ncd320d04c1 (HM317361) / human skin microbiome (Kong et al. unpublished) / 99
JQ512932 / >F57_10A / Uncultured bacterium ncd2603e02c1 (JF227954) / human skin microbiome (Kong et al. unpublished) / 99
JQ512933 / >F57_3C / ltured bacterium ncd2815f04c1 (JF241296) / human skin microbiome (Kong et al. unpublished) / 99
JQ512934 / >F57_5C / Uncultured ELU0149-T297-S-NIPCRAMgANa_000521 (HQ801691) / human (Li et al. unpublished) / 99
JQ512935 / >F57_2D / Uncultured bacterium nby242f08c1 (HM512020) / human (Grice et al. 2010) / 99
JQ512936 / >F57_5D / Pseudomonas sp. A1B27 (EU547454) / Antarctica (Tam and Wong, unpublished) / 99
JQ512937 / >F57_6D / Uncultured bacterium LI1_f03_1 (EU776549) / mammals gut microbes (Ley et al. 2008) / 99
JQ512938 / >F57_8D / Uncultured bacterium nby241h02c1 (HM811978) / human (Grice et al. 2010) / 98
JQ512939 / >F57_11D / Uncultured bacterium ncd2329h03c1 (JF204048) / human skin microbiome (Kong et al. unpublished) / 99
JQ512940 / >F57_6E / Uncultured bacterium ncd2798c04c1 (JF223787) / human skin microbiome (Kong et al. unpublished) / 99
JQ512941 / >F57_7E / Uncultured bacterium MB03F04 (FM873932) / house dust (Taubel et al. 2009) / 99
JQ512942 / >F57_8E / Uncultured bacterium MA04C11 (FM873766) / house dust (Taubel et al. 2009) / 99
JQ512943 / >F57_9E / Uncultured bacterium MA04C06 (FM873763) / house dust (Taubel et al. 2009) / 99
JQ512944 / >F57_3F / Uncultured bacterium ncd1281b05c1 (JF089423) / human (Kong et al. unpublished) / 99
JQ512945 / >F57_5F / Uncultured low G+C Gram-positive bacterium FAC36 (DQ451475) / soil (Tsai et al. unpublished) / 99
JQ512946 / >F57_8F / Uncultured bacterium ncd2744d05c1 (JF236775) / human skin microbiome (Kong et al. unpublished) / 99
JQ512947 / >F57_11F / Uncultured bacterium JPL2-50 (DQ532157) / clean rooms for spacecrafts (Moissl et al. 2007) / 100
JQ512948 / >F57_1G / Uncultured bacterium ncd1372f11c1 (JF120226) / human (Kong et al. unpublished) / 99
JQ512949 / >F57_4G / Uncultured bacterium HSD-c-Ce19 (JN698671) / pig/human feces (Ziemer et al. unpublished) / 100
JQ512950 / >F57_6G / Uncultured bacterium ncd2236e08c1 (JF193385) / human skin microbiome (Kong et al. unpublished) / 99
JQ512951 / >F57_7G / Uncultured bacterium MA04G12 (FM873793) / house dust (Taubel et al. 2009) / 99
JQ512952 / >F57_11G / Uncultured ELU0023-T00-S-NIPCRAMgANb_000402 (HQ747620) / human (Li et al. unpublished) / 99
JQ512953 / >F57_12G / Uncultured ELU0125-T311-S-NI_000028 (HQ791787) / human (Li et al. unpublished) / 100
JQ512954 / >F57_8H / Acinetobacter marinus (AY633607) / sea water (Yoon et al. 2007) / 99
JQ512955 / >F57_12H / Uncultured ELU0149-T297-S-NIPCRAMgANa (HQ801691) / human (Li et al. unpublished) / 100

References

Bik,E.M., Long,C.D., Armitage,G.C., Loomer,P., Emerson,J., Mongodin,E.F., Nelson,K.E., Gill,S.R., Fraser-Liggett,C.M.,Relman,D.A. (2010). Bacterial diversity in the oral cavity of 10 healthy individuals ISME Journal,4, 962-974.

Chen,Y.G., Cui,X.L., Pukall,R., Li,H.M., Yang,Y.L., Xu,L.H., Wen,M.L., Peng,Q.,Jiang,C.L. (2007). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China.International Journal of Systematic and Evolutionary Microbiology, 57, 2327-2332.

Doronina, N.V., Kaparullina, E.N., Trotsenko, Y.A. (2011). Methylovorus menthalis, a novel species of aerobic obligate Methylobacteria associated with plants.Microbiology,80, 713-719.

Grice,E.A., Kong,H.H., Conlan,S., Deming,C.B., Davis,J.,Young,A.C., Bouffard,G.G., Blakesley,R.W., Murray,P.R., Green,E.D.,Turner,M.L., & Segre,J.A. (2009). Topographical and temporal diversity of the human skin microbiome, Science,324, 1190-1192.

Grice, E.A., Snitkin, E.S., Yockey, L.J., Bermudez, D.M., Liechty, K.W., Serge, J.A. (2010). Longitudinal shift in diabetic wound microbiota correlates with prolonged skin defense response. Proceeding of theNationalAcademy of Sciences of theU.S.A.,107, 14799-14804.

Hantsis-Zacharov,E., Halpern,M. (2007). Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits Applied and Environmental Microbiology,73, 7162-7168.

Hunyadkurti, J., Feltoti, Z., Horvath, B., Nagymihaly, M., Voros, A., McDowell, A., Patrick, S., Urban, E., Nagy, I.(2011). Complete genome sequence of Propionibacterium acnes Type IB Strain 6609.Journal of Bacteriology,193, 4561-4562.

Inagaki,F., Nunoura,T., Nakagawa,S., Teske,A., Lever,M., Lauer,A., Suzuki,M., Takai,K., Delwiche,M., Colwell,F.S., Nealson,K.H., Horikoshi,K., D'Hondt,S.,Jorgensen,B.B. (2006). Biogeographical distribution and diversity of microbes in methane hydrate-bearing deep marine sediments on the Pacific Ocean Margin. Proceeding of the NationalAcademy of Sciences of the U.S.A., 103, 2815-2820.

Ishikawa,M., Tanasupawat,S., Nakajima,K., Kanamori,H., Ishizaki,S., Kodama,K., Okamoto-Kainuma,A., Koizumi,Y., Yamamoto,Y.,Yamasato,K. (2009). Alkalibacterium thalassium sp. nov., Alkalibacterium pelagium sp. nov., Alkalibacterium putridalgicola sp. nov. and Alkanibacterium kapii sp. nov. slightly halophilic and alkaliphilic marine lactic acid bacteria isolated from marine organisms and salted foods collected in Japan and Thailand. International Journal of Systematic and Evolutionary Microbiology, 59, 1215-1226.

Jones,R.T., McCormick,K.F.,Martin,A.P. (2008). Bacterial communities of Bartonella-positive fleas: diversity and community assembly patterns.Applied and Environmental Microbiology,74, 1667-1670.

Kanamoto, T., Sato, S., Inoue, M., (2000). Genetic heterogeneities and phenotypic characteristics of strains of the genus Abiotrophia and proposal of Abiotrophia para-adiacens sp. nov.Journal of Clinical Microbiology,38, 492-498.

Kato,Y., Sakala,R.M., Hayashidani,H., Kiuchi,A., Kaneuchi,C.,Ogawa,M. (2000).Lactobacillus algidus sp. nov., a psychrophilic lactic acid bacterium isolated from vacuum-packaged refrigerated beef.International Journal of Systematic and Evolutionary Microbiology, 50, 1143-1149.

Khachatryan,Z.A., Ktsoyan,Z.A., Manukyan,G.P., Kelly,D., Ghazaryan,K.A.,Aminov,R.I.(2008). Predominant role of host genetics in controlling the composition of gut microbiota.PLoS One,3, E3064.

Ley,R.E., Hamady,M., Lozupone,C., Turnbaugh,P.J., Ramey,R.R., Bircher,J.S., Schlegel,M.L., Tucker,T.A., Schrenzel,M.D., Knight,R.,Gordon,J.I. (2008). Evolution of mammals and their gut microbes.Science,320, 1647-1651.

Liu,F.H., Wang,S.B., Zhang,J.S., Zhang,J., Yan,X., Zhou,H.K., Zhao,G.P.,Zhou,Z.H. (2009). The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis.Journal of Applied Microbiology, 106, 952-966.

Lucquin, I., Zagorec, M., Champomier-Verges, M., Chaillou, S. (2012). Fingerprint of lactic acid bacteria population in beef carpaccio is influenced by storage process and seasonal changes. Food Microbiology,29, 187-196.

Moissl,C., Osman,S., La Duc,M.T., Dekas,A., Brodie,E., DeSantis,T.,Venkateswaran,K. (2007). Molecular bacterial community analysis of clean rooms where spacecraft are assembled.FEMS Microbiology Ecology,61, 509-521.

Reed,D.W., Fujita,Y., Delwiche,M.E., Blackwelder,D.B., Sheridan,P.P., Uchida,T.,Colwell,F.S. (2002). Microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin. Applied and Environmental Microbiology,68, 3759-3770.

Rintala,H., Pitkaranta,M., Toivola,M., Paulin,L.,Nevalainen,A. (2008). Diversity and seasonal dynamics of bacterial community in indoor environment.BMC Microbiology,8, 56.

Sahl,J.W., Schmidt,R., Swanner,E.D., Mandernack,K.W., Templeton,A.S., Kieft,T.L., Smith,R.L., Sanford, W.E., Callaghan,R.L., Mitton,J.B.,Spear,J.R. (2008). Subsurface microbial diversity in deep-granitic-fracture water in ColoradoApplied and Environmental Microbiology,74, 143-152.

Taubel,M., Rintala,H., Pitkaranta,M., Paulin,L., Laitinen,S., Pekkanen,J., Hyvarinen,A., Nevalainen,A. (2009).The occupant as a source of house dust bacteria. Journal of Allergy and ClinicalImmunology, 124, 834-840.

Yoon,J.H., Kim,I.G., Oh,T.K. (2007). Acinetobacter marinus sp. nov. and Acinetobacter seohaensis sp. nov., isolated from sea water of the Yellow Sea in Korea.Journal of Microbiology and Biotechnology,17, 1743-1750.

1