Sass et al. 2012lxa
SUPPLEMENTARY INFORMATION
The unexpected discovery of a novel low-oxygen activated locus for the anoxic persistence of Burkholderiacenocepacia
Andrea M. Sass,1Crystal Schmerk,2 Kirsty Agnoli,3 Phillip J. Norville,1 Leo Eberl,3 Miguel A. Valvano 2,4 and Eshwar Mahenthiralingam1*
Affiliations:
1 Organisms and Environment Division, Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff, Wales, UK, CF10 3AT
2 Current address: Laboratorium voor Farmaceutische Microbiologie, Universiteit Gent, Gent, Belgium
3 Centre for Human Immunology, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada N6A 5C1,
4 Department of Microbiology, Institute of Plant Biology, University of Zürich, Zollikerstrasse 107. CH-8008, Zürich, Switzerland.
5 Centre for Infection and Immunity, Queen’s University of Belfast, Belfast, UK, BT9 7BL
Corresponding author: Prof. Eshwar Mahenthiralingam
email:
SUPPLEMENTARY METHODS
Cultivation of B. cenocepacia J2315 for microarray experiments
Modelling of B. cenocepacia growth conditions was carried out as follows. For low pH conditions, the pHused was 5.5, as B.cenocepacia J2315 was found not to grow consistently at pH 5 or lower. LB (Luria Bertani broth, Sigma-Aldrich L7275) broth and a basal salt medium (BSM, Hareland et al 1975) with 0.4% glucose and 0.05% yeast extract and casamino acids were used as growth media. For BSM pH5.5, the proportion of phosphate salts was adjusted to (in g/L): 1.65 KH2PO4, 0.35 NaH2PO4, 0.15 K2HPO4x3H2O, 0.017 Na2HPO4. For BSM with reduced iron content, FeSO4 was omitted. The iron concentration was determined by Atomic Absorption Spectrophotometry (Spect AA-100, Varian). Samples were acidified with nitric acid to a pH <3 prior to measurement. Cultivation was performed as described in Sass et al (Sass et al 2011) with cells harvested at O.D.600 0.5, equivalent to 5x108CFU/ml. Stationary phase cultures were harvested after a fixed time, 18 hours in LB broth (O.D.600 6.0) and 30 hours in BSM (O.D.600 4.0).
To expose the cultures to heat stress, cells were grown at 37ºC to an O.D.600 of 0.4 to 0.45 and then incubated for one hour at 42.5ºC. To expose cultures to oxidative stress, cells were grown at 37ºC to an O.D.600 of 0.5. Then 25μl of a 1% solution of tert-butyl hydroperoxide (0.001% final concentration) or 125μl of a 30% hydrogen peroxide solution (0.15% final concentration) were added to the culture and incubated for 15min. For experiments at reduced oxygen concentration, cells were grown in LB to an O.D.600 of 0.3-0.4, transferred into a 50ml centrifugation tube and placed upright into a CampyGen Compact (Oxoid, Basingstoke, UK) plastic pouch containing the gas generating paper sachet. The oxygen concentration within the plastic pouch was reduced to 6 % by replacing oxygen with carbon dioxide. The culture was further incubated at 37ºC in a shaking incubator at 150rpm for 2 to 3 hours and then harvested at an O.D.600 of 0.5. To harvest the cells with minimal exposure to atmospheric oxygen concentration prior to pelleting, the centrifuge tube was sealed before opening the pouch. The culture was then snap-cooled in liquid nitrogen within the sealed tube, before opening the centrifuge tube and aliquoting the culture into smaller tubes for harvesting. All experiments were performed with three biological replicates and sustained viability was confirmed by drop-counts of every culture.
RNA extraction and labelling
Cultures were swiftly aliquoted into microcentrifuge tubes and snap-cooled in liquid nitrogen before centrifuging at 20.000 x g at 4ºC for 1 min. Pellets were immediately frozen at 80ºC and RNA was extracted within one week of harvest using the RiboPure Bacteria Kit (Ambion/Applied Biosystems). Extracted RNA was treated with the DNase I included in the kit, then pooled and purified via precipitation with LiCl (Ambion/Applied Biosystems). The RNA quality was assessed with a Bioanalyzer (Agilent) using the RNA 6000 Nano kit (Agilent). Samples generally had an RNA Integrity Number of >9.0, which signifies absence of RNA degradation.RNA was labeled with the CyScribe Post-Labelling Kit (GE Healthcare), which provides an indirect method of labelling, to minimise any dye bias. First strand cDNA is generated using random nonamers and incorporating amino allyl-dUTPs, which are chemically labelled in a second step. 10μg of total RNA were used per labeling reaction and Spike-In controls (Agilent) were added to the labelling mix.
DNA extraction and labelling
Genomic DNA was extracted by mechanical disruption using bead-beater. 3 ml of an over-night broth culture were harvested, mixed with lysis buffer containing pronase and glass beads, and beat for 10 sec on the bead-beater. The lysate was incubated at 37ºC for 1 hour and mixed with saturated ammonium acetate and chloroform. The mix was centrifuged, the upper aqueous phase removed and the DNA purified by ethanol precipitation. Purified DNA was further treated with RNase.The DNA was then labelled with the CyScribe Array CGH Genomic DNA Labeling System (GE Healthcare, UK) at 1 μg per labelling reaction, according to manufacturer’s instructions.
Hybridization and scanning
A custom 4 x 44k microarray for B. cenocepacia was manufacuted by Agilent Technologies using their high-density 60-mer SurePrint technology. The microarray included 8740 probes derived from the J2315 genome, 7251 probes for coding sequences and 1489 probes for intergenic regions, each printed four times in randomised order. The array design has been deposited at ArrayExpress under the accession number A-MEXP-1613. Microarrays were hybridized according to the Two-Colour Microarray Based Gene Expression Analysis protocol (Agilent, UK), adjusted for the use of cDNA: the fragmentation step was omitted and instead the hybridization mix was denatured at 98ºC for 3 min. Labelled cDNA was used at 825 ng per sample, labelled genomic DNA was used at 200 ng per sample. The washing procedure included the use of Stabilizing and Drying Solution (Agilent) to prevent ozone-related degradation of Cy5. Microarray slides were scanned with a microarray scanner (G2565 BA, Agilent) with the Scan Control software version A.7.0.3 Feb 2007 (Agilent) and a scan resulution of 5 μm. The ExtendedDynamicRange function with 100% and 10% PMT gain was enabled.
Microarray analysis
Scanner images were analysed with the Feature Extraction version 9.5.1 (Agilent) using the FE protocol GE2_v5_95. Data was then imported into GeneSpring GX version 7.3.1. (Agilent) Normalisation was performed as follows: First every spot of the signal channel was divided by the control channel. Each chip was then normalised to the 50th percentile of all measurements of that chip followed by normalisation of each gene to its median. For analysis of differential gene expression, first a filter on 1.5-fold change in expression in the test condition compared to the respective control condition was applied. Then a one-way ANOVA was carried out on the resulting gene lists, using a Wech t-test with 5% false discovery rate. Values for all four replica spots for each probe were averaged during the process.Differences in gene expression are based on mean values from three biological replicates, and p-values resulting from ANOVA represent a measure of variation between replicates.
qPCR for validation of microarray results was performed as described (Sass et al 2011). The qPCR primers are listed in Supplementary Table1 and an annealing temperature of 60ºC was used for all primers. Four genes with stable transcript levels were used as control genes. Fold changes were calculated according to the delta-delta CT method as described (Sass et al 2011).
SUPPLEMENTARY RESULTS
Genes with unknown function
The expression of predicted genes annotated as hypothetical proteins, conserved hypothetical proteins or uncharacterised membrane/exported proteins changed under all conditions tested (Table2). The proportion of such genes within the entire genome (2850 CDS; 25.6 %)was comparable to their proportion of the total regulated genes, showing that predicted and uncharacterised genes were not disproportionately represented in the data. Uncharacterised genes were frequently among the highest upregulated genes.For example: the most upregulated gene at stationary phase on minimal medium was the conserved hypothetical protein BCAL0928 (805 fold); and in LB medium at stationary phase BCAL0434, a putative exported protein was most highly expressed (273 fold; Supplementary Table S3). The gene cluster BCAL1663-1665 comprised poorly characterised genes consistently induced under all stress conditions that resulted in growth arrest. The cluster contained a serine protein kinase (BCAL1663), a conserved hypothetical protein (BCAL1664), and a SpoVR-like protein (BCAL1665), these were present in the same conserved gene order in all sequenced Burkholderia species, and in unrelated gram negative bacteria like Pseudomonas spp.
The tandem hypothetical genes, BCAS0292-0293, each with a domain associated with inclusion body proteins in Xenorhabdus nematophila (PixA, (Goetsch et al 2006), also exhibited high fold changes in expression under growth arrest conditions (24 to 319 fold;Supplementary Table S3). They were co-regulated with quorum-sensing controlled extracellular protease ZmpA (BCAS0409), and were strongly upregulated in stationary phase with less induction under low oxygen and heat stress conditions. A second cluster of PixA domain-containing genes, BCAM1412-1414, also displayed the same pattern of upregulation during stationary phase and under low oxygen conditions, but with lower fold change. All five homologous PixA domain-containing genes occurwith high similarity in otherB.cenocepacia strains, and two in B. lata sp. 383.
Significance of hypothetical genes
Gene cluster BCAL1663-1665, ubiquitous in all sequenced Burkholderia spp. and consistently induced under all stress conditions associated with growth arrest, potentially plays an essential role in stress response and persistence. Homologues of these gene products are under sporulation control in Bacillussubtilis(Fawcett et al 2000) and are induced under oxygen depletion in P.aeruginosa (Alvarez-Ortega and Harwood 2007), suggesting that in these organisms these proteins are also associated with adaptation to growth arrest. Despite this seemingly universal occurrence and role, these proteins remain uncharacterised.
The phylogenetic distribution of the five hypothetical genes encoding PixA domain-containing proteins (BCAS0292-0293, BCAM1412-1414) and their expression pattern suggests they are involved in a B.cenocepacia-specific response to growth arrest associated stress. One of these genes, encoding the hypothetical protein AidA (BCAS0293) has been is expressed in stationary phase in B. cenocepacia H111 (Huber et al 2004), it is under regulatory control by the quorum sensing system CepI/R in B.cenocepacia K56-2 and H111(Chambers et al 2006; Uehlinger et al 2009; Inhülsen et al 2012), and aB.cenocepacia H111 aidA deletion mutant showed attenuation in slow killing of Caenorhabditis elegans, but not in other infection models tested (Uehlinger et al 2009). Deletion of the aidA-containing operon (BCAS0292-0293) in B.cenocepacia strains K56-2 (Sass et al 2011) and H111 (Huber et al 2004) did not reduce survival rates upon starvation. Therefore, the operon is generally not essential for survival of growth arrest despite its strong induction when growth arrest occurs. On the other hand, the second gene cluster with genes encoding PixA domain-containingproteins (BCAM1412-1414) could have compensated for the loss of BCAS0292-0293.
Regulation of transposases
Genes with expression linked to both organic and in-organic oxidative stress (Table 1) were transposases within ISBcen20 type insertion sequences (Supplementary Table 1; Drevinek et al 2010, Holden et al 2009). Other transposases with growth condition specific regulation were type ISBcen5 transposases that were induced at elevated temperature and type ISBcen8 that were upregulated in stationary phase.Mobile elements like phage components and transposases are also induced. Genome rearrangements, linked to transposase activity, can be caused by oxidative stress in B.cenocepacia strains of the same type as J2315 (Drevinek et al. 2010). In our study, transposases were induced in several test conditions, which suggests that many environmental stressors occurring during host colonisation may lead to increased genome rearrangements and mutability in B.cenocepacia J2315.
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Oxidative stress of Burkholderiacenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. J Clin Microbiol48: 34-40.
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a novel virulence factor in Burkholderiacenocepacia H111 required for efficient slow killing
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linking quorum sensing with biofilm formation in Burkholderiacenocepacia H111.
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SUPPLEMENTARY TABLES
Supplementary Table S1. Primers used in this study
Supplementary Table S2. Validation of B. cenocepacia gene expression by qPCR
Supplementary Table S3. Alterations in expression for selected B. cenocepacia genes
(separate Microsoft Excel spreadsheet)
Supplementary Table S4. All significant gene expression changes observed in the B. cenocepacia transcriptomic reference dataset expressed as fold change (separate Microsoft Excel spreadsheet)
Supplementary Table S5. Sequences used to generate the consensus sequence of the putative lxamotif
Supplementary Table S6. List of orthologs of lxa and low oxygen coregulated genes in other available Burkholderia genomes
Supplementary Table S7. Carbon and nitrogen source profiling data for the B. cenocepacia K56-2 lxa mutant
Supplementary Table S1. Primers used in this study
qPCR primersControl genes / Forward and reverse primer / Product size (bp)
BCAM0918 / rpoD / GAGATGAGCACCGATCACAC / 143
CCTTCGAGGAACGACTTCAG
BCAL0330 / cyt c1 / GACAAGACGGACGAGGAGAC / 196
GGACACCGAGAAAGATCAGC
BCAL2176 / dihydro-dipicolinate / TTTCGCAAACTGATCGACTG / 109
synthase / TCATCAGGATGTGCTCTTCG
BCAL0026 / parA / TATGAAGTGCTGGTCGATGG / 190
TCAGCACGAAATCGTAGTCG
Test genes / Forward and reverse primer / Product size (bp)
BCAL0114 / Flagellin / TTGCACAGCAGAACCTCAAC / 150
GGTTCAGACCGTTGATCTGG
BCAL1664 / Conserved / TTCTGCCTGATGGACGTATC / 177
hypothetical protein / TCGAATGGAAGAAGGTGTCC
BCAL2118 / Isocitrate lyase / GTCAAGCGCATCAACAACAC / 206
AGCTGGTCCTCGAAGTGAAC
BCAM0276 / uspA / CATCATGGTCGCAGTAGACG / 133
GCCGTAAGCAAACAGAATGG
BCAM1870 / cepI / AAAGTTTCGAGCGTGACCAG / 128
CAGCGACTTCAGCAGATACG
Primers for mutagenesis of B. cenocepacia K56-2 and J2315
Forward and reverse primer; restriction endonuclease sites are underlined / Restriction enzyme
Upstream BCAM0275 / lowoxy cluster BCAM0275 F / ATCGATAATATTGAGGCGCATTATGCACTCGACAAC / SspI
Upstream BCAM0275 / lowoxy cluster BCAM0275 R / GATCGAGGTACCCTGTCGACGACGAGGTCCACACAC / KpnI
Downstream BCAM0323 / lowoxy cluster BCAM0323 F / GATCGAGGTACCTTACAGATCGGACCGAAAGGACAT / KpnI
Downstream BCAM0323 / lowoxy cluster BCAM0323 R / GTCGCATCTAGAATCGTTCACACGCATGATCTGTCC / XbaI
Supplementary Table S2. Validation of B. cenocepacia gene expression by qPCR
Condition / Gene and annotation / Fold changeLow oxygen concentration / qPCR / microarray
BCAL0114 / Flagellin / 5.3 / 6.1
BCAL1664 / Conserved hypothetical protein / 21.6 / 9.5
BCAL2118 / Isocitrate lyase / 30.3 / 11.2
BCAM0276 / Universal stress protein UspA / 438.1 / 58.9
BCAM1870 / N-acylhomoserine lactone synthase CepI / 5.9 / 3.8
Stationary phase in minimal medium with glucose
BCAL0114 / Flagellin / -340.5 / -588.2
BCAL1664 / Conserved hypothetical protein / 32.8 / 30
BCAL2118 / Isocitrate lyase / 322.2 / 64.3
BCAM0276 / Universal stress protein / 0.9 / no change
BCAM1870 / N-acylhomoserine lactone synthase CepI / 5.4 / 3.2
Oxidative stress (organic peroxide; hydrogen peroxide 0.15%)
BCAL0114 / Isocitrate lyase / -1.3 / no change
BCAL1664 / Conserved hypothetical gene / 4.7 / 5.2
BCAM1870 / N-acylhomoserine lactone synthase CepI / -24.1 / -4.4
Supplementary Table S5. Sequences used to generate the consensus sequence of the putative lxamotif
Gene ID / Strand / Start / p-value / SequenceConsensus / TTGACG[CG]A[GC][AG]TCAA[CG]
BCAM0285 / + / 74 / 3.43E-09 / TTGACGCACATCAAG
BCAM0294 / + / 67 / 3.43E-09 / TTGACTCAGATCAAC
BCAM0306 / - / 104 / 3.43E-09 / TTGATGCAGATCAAC
BCAM0301 / - / 106 / 1.14E-08 / TTGATACAGATCAAC
BCAM0319 / - / 66 / 1.36E-08 / TTGACGGAGGTCAAC
BCAM0317 / + / 62 / 2.49E-08 / TTGACTCACGTCAAG
BCAM1480 / + / 50 / 3.57E-08 / TTGACCCACGTCAAC
BCAM0314 / + / 127 / 4.09E-08 / TTGACTCATGTCAAG
BCAM0278 / - / 147 / 4.09E-08 / TTGACGCGCATCAAG
BCAM0289 / + / 71 / 4.09E-08 / TTGACGCGGGTCAAG
BCAM0279 / - / 83 / 4.85E-08 / TTGACGGAAGTCAAC
BCAM0316 / + / 139 / 5.08E-08 / TTGACGCAGATCATG
BCAM1570 / - / 57 / 5.08E-08 / TTGACGCGTATCAAC
BCAM0275a / - / 185 / 5.81E-08 / TTGACACAGGTCAAT
BCAM1494 / - / 116 / 5.81E-08 / TTGACGCGCGTCAAC
BCAM0276 / + / 78 / 5.81E-08 / TTGACCGAGGTCAAC
BCAL1249 / + / 80 / 1.68E-07 / CTGATGCACATCAAG
BCAM0310 / + / 61 / 3.18E-07 / TTGACGGCGGTCAAC
BCAM0305 / - / 95 / 3.39E-07 / GTGATACAGATCAAG
BCAM0300 / + / 65 / 5.61E-07 / TTGACGGAAATCAGG
BCAM0312 / + / 54 / 9.36E-07 / CTGAGCCAGATCAAC
1
Sass et al. 2012lxa
Supplementary Table 6. List of orthologs of lxa and low oxygen coregulated genes in other available Burkholderia genomes
Gene ID / B. vietnamiensis G4 / B. multivorans ATCC17616 / B. ambifaria MC40-6 / B. ambifaria AMMD / B. dolosa AU0158 / B. pseudomallei K96243 / B. malleiATCC 23344 / B. thailandensis E264 / B. xeno-vorans LB400
BCAM0275a / Bcep1808_6355 / Bmul_5917 / BamMC406_6184 / Bxe_A1865
BCAM0276 / Bcep1808_6354 / Bmul_5916 / BamMC406_6185 / Bamb_5714 / BDAG_04861 / BPSS1934 / BMAA0144 / BTH_II0442 / Bxe_A1859
BCAM0277 / Bcep1808_6353 / Bmul_5915 / BamMC406_6187 / Bamb_3761 / BDAG_03727 / BPSS0833 / BMAA0701 / BTH_II1572 / Bxe_A1843
BCAM0278 / Bcep1808_6352 / Bmul_5914 / BamMC406_6186 / BDAG_04860 / BPSS2288 / BMAA2035 / Bxe_A1869
BCAM0279 / Bcep1808_6351 / Bmul_5913 / BamMC406_6189 / BDAG_04859 / BPSS2287 / BMAA2038 / BTH_II2318 / Bxe_A1863
BCAM0280 / Bcep1808_6350 / Bmul_5912 / BamMC406_6188 / BDAG_04858 / BPSS1924 / BMAA0155 / Bxe_A1870
BCAM0280a / Bcep1808_4885 / Bmul_5911 / BamMC406_6190 / Bamb_3762 / BDAG_03293 / BPSS0834 / BMAA0702 / BTH_II1571 / Bxe_A1871
BCAM0281 / Bcep1808_6344 / Bmul_5910 / BamMC406_4990 / Bamb_4465 / BDAG_04857 / BPSS2285 / BMAA2031 / BTH_II2316
BCAM0282 / Bcep1808_6343 / Bmul_5909 / BamMC406_6191 / Bamb_3787 / BPSS1923 / BMAA0157
BCAM0283 / Bcep1808_6342 / Bmul_5908 / BamMC406_6192 / BDAG_04856 / BPSS1922 / BMAA0158 / BTH_II0454
BCAM0284 / Bcep1808_6341 / Bmul_5907 / BamMC406_6193 / BDAG_04855 / BTH_II0457 / Bxe_B1842
BCAM0285 / Bcep1808_6340 / Bmul_5906 / BamMC406_6194 / BDAG_04854 / BPSS2284 / BTH_II2315 / Bxe_B1864
BCAM0286 / Bcep1808_6339 / Bmul_5905 / BamMC406_4279 / Bamb_3811 / BDAG_04853 / BPSS1918 / BMAA0163 / BTH_II0459 / Bxe_A1844
BCAM0287 / Bcep1808_6338 / Bmul_5904 / BamMC406_6195 / Bamb_3812 / BDAG_04852 / BPSS1917 / BMAA0164 / BTH_II0460 / Bxe_B0606
BCAM0288 / Bcep1808_6337 / Bmul_5903 / BamMC406_6196 / Bamb_3774 / BDAG_04850 / BPSS0835 / BMAA0703 / BTH_II1570 / Bxe_A1850
BCAM0289 / Bcep1808_4895 / Bmul_5902 / BamMC406_6197 / Bamb_3775 / BDAG_04849 / Bxe_A1851
BCAM0290 / Bcep1808_6336 / Bmul_5901 / BamMC406_6198 / Bamb_3813 / BDAG_04848 / BPSS0836 / BMAA0705 / BTH_II1569 / Bxe_A3827
BCAM0291 / Bcep1808_6335 / Bmul_5900 / BamMC406_6199 / Bamb_3814 / BDAG_04847 / BPSS0837 / BMAA0706 / BTH_II1568 / Bxe_A1852
BCAM0292 / Bcep1808_6334 / Bmul_5899 / BamMC406_6200 / Bamb_3815 / BDAG_04846 / BPSS0838 / BMAA0707 / BTH_II1567 / Bxe_A3827
BCAM0293 / Bcep1808_6333 / Bmul_5898 / BamMC406_4284 / Bamb_3816 / BDAG_04845 / BPSS1956 / BMAA0120 / BTH_II0416 / Bxe_B0356
BCAM0294 / Bcep1808_6332 / Bmul_5897 / BamMC406_4285 / Bamb_3817 / BDAG_04844 / BPSS0839 / BMAA0708 / BTH_II1566 / Bxe_A1854
BCAM0295 / Bcep1808_6331 / Bmul_5896 / BamMC406_4286 / Bamb_3818 / BDAG_04843
BCAM0296 / Bcep1808_6330 / Bmul_5895 / BamMC406_4287 / Bamb_3819 / BDAG_04842 / BPSS1916 / BMAA0166 / BTH_II0461 / Bxe_A2341
BCAM0297 / Bcep1808_6329 / Bmul_5894 / BamMC406_4288 / Bamb_3820 / BDAG_04841 / BPSS1954 / BMAA0122 / BTH_II0418 / Bxe_C0053
BCAM0298 / Bcep1808_6175 / Bmul_5893 / BamMC406_4289 / Bamb_3821 / BDAG_04840 / BPSS1955 / BMAA0121 / BTH_II0417 / Bxe_C0052
BCAM0299 / Bcep1808_6328 / Bmul_5892 / BamMC406_4290 / Bamb_3822 / BDAG_04839 / BPSS0840 / BMAA0709 / BTH_II1565 / Bxe_A1860
BCAM0300 / Bcep1808_6325 / Bmul_5891 / BamMC406_6202 / Bamb_3826 / BDAG_04838 / BPSS1915 / BMAA0168 / BTH_II0462
BCAM0301 / Bcep1808_6319 / Bmul_5885 / BamMC406_6204 / Bamb_3823
BCAM0302 / Bcep1808_6318 / Bmul_5884 / BamMC406_6205 / BPSS1939 / BMAA0138 / BTH_II0437 / Bxe_B2341
BCAM0303 / Bcep1808_6317 / Bmul_5883 / BamMC406_6206 / BPSS1938 / BMAA0139 / BTH_II0438 / Bxe_B2340
BCAM0304 / Bcep1808_6316 / Bmul_5882 / BamMC406_6207 / BPSS1937 / BTH_II0439 / Bxe_B2339
BCAM0305 / Bcep1808_6315 / Bmul_5881 / BamMC406_6208 / BPSS1936 / BMAA0141 / BTH_II0440 / Bxe_B2338
BCAM0306 / Bcep1808_6319 / Bmul_5880 / BamMC406_6204 / Bamb_3823
BCAM0307 / Bcep1808_6313 / Bmul_5879 / BamMC406_6209 / BDAG_04837 / BPSS1958 / BMAA0116 / BTH_II0414 / Bxe_A1899
BCAM0308 / Bcep1808_6312 / Bmul_5878 / BamMC406_6210 / BDAG_04836 / BPSS1115 / BTH_II1297 / Bxe_A1900
BCAM0309 / Bcep1808_6311 / Bmul_5877 / BamMC406_6211 / Bamb_3874 / BDAG_04835 / BPSS1114 / BMAA1040 / Bxe_A1901
BCAM0310 / Bcep1808_6310 / Bmul_5876 / BamMC406_6212 / BDAG_04834 / BPSS2282 / BMAA2027 / BTH_II2313 / Bxe_B1704
BCAM0311 / Bcep1808_6309 / Bmul_5875 / BamMC406_6213 / BDAG_04833 / BPSS1957 / BMAA0117 / BTH_II0415
BCAM0312 / Bcep1808_6308 / Bmul_5874 / BamMC406_6214 / BDAG_04832 / BPSS2302 / BMAA2052 / BTH_II2322
BCAM0313 / Bcep1808_6307 / Bmul_5873 / BamMC406_6215 / BDAG_04831 / BPSS2303 / BMAA2054 / BTH_II0926
BCAM0314 / Bcep1808_6306 / Bmul_5872 / BamMC406_5575 / Bxe_B0479
BCAM0315 / Bcep1808_6304 / Bmul_5871 / BamMC406_6217 / BDAG_04830 / BPSS1101 / BTH_II1308
BCAM0316 / Bcep1808_6302 / BamMC406_6221
BCAM0317 / Bcep1808_6294 / Bmul_5861 / BamMC406_6232 / BDAG_04829 / BPSS1099 / BTH_II1310
BCAM0318 / Bcep1808_6293 / Bmul_5860 / BamMC406_6233 / BDAG_04828
BCAM0319 / Bcep1808_6292 / Bmul_5859 / BamMC406_6234 / BDAG_04827 / BPSS1140 / BTH_II1268 / Bxe_A1890
BCAM0320 / Bcep1808_6290
BCAM0321 / Bcep1808_6289
BCAM0322 / Bcep1808_6288
BCAM0323 / Bcep1808_6287
BCAL1249 / Bcep1808_1232 / Bmul_2041 / BamMC406_1168 / Bamb_1156 / BDAG_01958 / BPSS2281 / BMAA2026 / BTH_II2312
BCAM1480 / Bcep1808_4884 / BamMC406_4235 / Bamb_3761 / BPSS0833 / BMAA0701 / BTH_II1572 / Bxe_A1843
BCAM1481 / Bcep1808_4885 / Bmul_4266 / BamMC406_4236 / Bamb_3762 / BDAG_03293 / BPSS0834 / BMAA0702 / BTH_II1571 / Bxe_A1871
BCAM1482 / Bcep1808_4886 / BamMC406_4237 / Bamb_3763 / BPSS1921 / BMAA0159 / BTH_II0455 / Bxe_A1858
BCAM1493 / Bcep1808_4894 / Bmul_4256 / BamMC406_4248 / Bamb_3774 / BDAG_04850 / BPSS0835 / BMAA0703 / BTH_II1570 / Bxe_A1850
BCAM1494 / Bcep1808_4895 / Bmul_4255 / BamMC406_4249 / Bamb_3775 / BDAG_04849 / BPSS1160 / BTH_II1248 / Bxe_A1851
BCAM1495 / Bcep1808_4896 / Bmul_4254 / BamMC406_4250 / Bamb_3776 / BDAG_03303 / BPSS0836 / BMAA0705 / BTH_II1569 / Bxe_A1888
BCAM1496 / Bcep1808_4897 / Bmul_4253 / BamMC406_4251 / Bamb_3777 / BDAG_03304 / Bxe_A0553
BCAM1570 / Bcep1808_4991 / BamMC406_4330 / Bamb_3870 / BPSS1944 / BMAA0132 / BTH_II0428 / Bxe_A1872
BCAM1571 / Bcep1808_4992 / Bmul_4185 / BamMC406_4331 / Bamb_3871 / BDAG_03386 / BPSS1850 / BMAA0251 / BTH_II0527
Gene ID / B. ceno-cepacia AU1054 / B. cenocepacia HI2424 / B. cenocepacia MC0-3 / B. ceno-cepacia PC184 / B. ceno-cepacia H111 / B. lata sp. 383 / B. multivorans CGD2 / B. multivorans CGD2M
BCAM0275a
BCAM0276 / Bcen_1440 / Bcen2424_6388 / Bcenmc03_7052 / BCPG_5572 / I35_1082 / Bcep18194_C6944 / BURMUCGD2_6136 / BURMUCGD2M_6125
BCAM0277 / Bcen_4015 / Bcen2424_4352 / Bcenmc03_6002 / BCPG_3273 / I35_6419 / Bcep18194_B1668 / BURMUCGD2_4158 / BURMUCGD2M_4147
BCAM0278
BCAM0279
BCAM0280
BCAM0280a / Bcen_4014 / Bcen2424_4353 / Bcenmc03_6001 / BCPG_3272 / I35_1081 / Bcep18194_B1667 / BURMUCGD2_4870 / BURMUCGD2M_4862
BCAM0281 / BURMUCGD2_2130 / BURMUCGD2M_2216
BCAM0282 / Bcen_3994 / Bcen2424_4373 / Bcenmc03_5982 / BCPG_3253 / Bcep18194_B1644
BCAM0283
BCAM0284
BCAM0285
BCAM0286
BCAM0287 / Bcen_4012 / Bcen2424_4355 / Bcenmc03_5990 / BCPG_3270 / I35_1079 / Bcep18194_B1665 / BURMUCGD2_4869 / BURMUCGD2M_4861
BCAM0288 / Bcen_4002 / Bcen2424_4365 / Bcenmc03_5989 / BCPG_3260 / I35_1068 / Bcep18194_B1652 / BURMUCGD2_4860 / BURMUCGD2M_4851
BCAM0289 / Bcen_4001 / Bcen2424_4366 / Bcenmc03_5988 / BCPG_3259 / I35_1067 / Bcep18194_B1651 / BURMUCGD2_4859 / BURMUCGD2M_4850
BCAM0290 / Bcen_4000 / Bcen2424_4367 / Bcenmc03_5987 / BCPG_3258 / I35_1066 / Bcep18194_B1650 / BURMUCGD2_4858 / BURMUCGD2M_4849
BCAM0291
BCAM0292 / I35_0662
BCAM0293 / Bcen_3495 / Bcen2424_4871 / Bcenmc03_5415 / BCPG_5066 / Bcep18194_B2633
BCAM0294
BCAM0295
BCAM0296 / Bcen_2157 / Bcen2424_2771 / Bcenmc03_2782 / BCPG_1990 / I35_6753 / Bcep18194_A6101 / BURMUCGD2_0690 / BURMUCGD2M_0780
BCAM0297
BCAM0298 / Bcen_3494 / Bcen2424_4872 / Bcenmc03_5414 / BCPG_5065 / Bcep18194_B2634
BCAM0299
BCAM0300
BCAM0301
BCAM0302
BCAM0303
BCAM0304
BCAM0305
BCAM0306
BCAM0307
BCAM0308
BCAM0309 / Bcen_3924 / Bcen2424_4443 / Bcenmc03_5863 / BCPG_3190 / I35_0977 / Bcep18194_B1520 / BURMUCGD2_4777 / BURMUCGD2M_4766
BCAM0310
BCAM0311
BCAM0312
BCAM0313
BCAM0314
BCAM0315
BCAM0316
BCAM0317
BCAM0318
BCAM0319
BCAM0320
BCAM0321
BCAM0322
BCAM0323
BCAL1249 / Bcen_0797 / Bcen2424_1278 / Bcenmc03_1250 / BCPG_0494 / I35_5342 / Bcep18194_A4421 / BURMUCGD2_2428 / BURMUCGD2M_2514
BCAM1480 / Bcen_4015 / Bcen2424_4352 / Bcenmc03_6002 / BCPG_3273 / I35_1082 / Bcep18194_B1668
BCAM1481 / Bcen_4014 / Bcen2424_4353 / Bcenmc03_6001 / BCPG_3272 / I35_1081 / Bcep18194_B1667 / BURMUCGD2_4870 / BURMUCGD2M_4862
BCAM1482 / Bcen_4013 / Bcen2424_4354 / Bcenmc03_6000 / BCPG_3271 / I35_1080 / Bcep18194_B1666
BCAM1493 / Bcen_4002 / Bcen2424_4365 / Bcenmc03_5989 / BCPG_3260 / I35_1068 / Bcep18194_B1652 / BURMUCGD2_4860 / BURMUCGD2M_4851
BCAM1494 / Bcen_4001 / Bcen2424_4366 / Bcenmc03_5988 / BCPG_3259 / I35_1067 / Bcep18194_B1651 / BURMUCGD2_4859 / BURMUCGD2M_4850
BCAM1495 / Bcen_4000 / Bcen2424_4367 / Bcenmc03_5987 / BCPG_3258 / I35_0662 / Bcep18194_B1650 / BURMUCGD2_4858 / BURMUCGD2M_4849
BCAM1496 / Bcen_3999 / Bcen2424_4368 / Bcenmc03_5986 / BCPG_3257 / I35_1065 / Bcep18194_B1649 / BURMUCGD2_4857 / BURMUCGD2M_4848
BCAM1570 / Bcen_3928 / Bcen2424_4438 / Bcenmc03_5867 / BCPG_3194 / I35_0984
BCAM1571 / Bcen_3927 / Bcen2424_4439 / Bcenmc03_5866 / BCPG_3193 / I35_0983 / Bcep18194_B1524 / BURMUCGD2_4782 / BURMUCGD2M_4771
Supplementary Table 7. Carbon and nitrogen source profiling data for the B. cenocepacia K56-2 lxa mutant
O. D. > 1.5 fold higher in wt / O. D. > 1.5 fold higher in mutantCarbon source / K56-2 1st run / K56-2 2nd run / Low oxygen condition
Plate / Location / Chemical / KEGG ID / OD WT / OD mutant / OD WT / OD mutant / OD WT / OD mutant
PM01 / A01 / Negative Control / 0.32 / 0.20 / 0.25 / 0.18 / 0.18 / 0.15
PM01 / A02 / L-Arabinose / C00259 / 1.24 / 1.89 / 1.50 / 1.68 / 1.54 / 1.88
PM01 / A03 / N-Acetyl-D-Glucosamine / C00140 / 0.38 / 0.23 / 0.27 / 0.20 / 0.14 / 0.15
PM01 / A04 / D-Saccharic acid / C00818 / 1.87 / 1.79 / 2.15 / 2.42 / 2.31 / 2.94
PM01 / A05 / Succinic acid / C00042 / 1.73 / 1.57 / 1.69 / 2.05 / 1.29 / 2.06
PM01 / A06 / D-Galactose / C00124 / 1.56 / 1.80 / 1.64 / 2.04 / 1.82 / 2.02
PM01 / A07 / L-Aspartic acid / C00049 / 1.56 / 2.41 / 1.82 / 1.92 / 2.12 / 2.38
PM01 / A08 / L-Proline / C00148 / 1.31 / 1.39 / 1.67 / 1.08 / 2.13 / 2.40
PM01 / A09 / D-Alanine / C00133 / 0.86 / 0.61 / 0.86 / 0.57 / 1.89 / 1.48
PM01 / A10 / D-Trehalose / C01083 / 1.24 / 1.25 / 1.44 / 0.91 / 1.90 / 1.58
PM01 / A11 / D-Mannose / C00159 / 0.85 / 0.69 / 0.81 / 0.92 / 2.94 / 2.44
PM01 / A12 / Dulcitol / C01697 / 1.12 / 1.45 / 1.54 / 1.34 / 2.02 / 1.96
PM01 / B01 / D-Serine / C00740 / 0.33 / 0.28 / 0.36 / 0.22 / 0.28 / 0.23
PM01 / B02 / D-Sorbitol / C00794 / 1.33 / 1.59 / 1.42 / 1.54 / 1.42 / 1.34
PM01 / B03 / Glycerol / C00116 / 1.00 / 0.62 / 0.91 / 0.77 / 1.08 / 0.89
PM01 / B04 / L-Fucose / C01019 / 1.29 / 1.77 / 1.39 / 1.55 / 1.59 / 1.68
PM01 / B05 / D-Glucuronic acid / C00191 / 0.74 / 0.46 / 0.74 / 0.36 / 2.47 / 2.15
PM01 / B06 / D-Gluconic acid / C00257 / 1.58 / 2.27 / 1.86 / 2.43 / 1.86 / 1.65
PM01 / B07 / DL-a-Glycerol Phosphate / C00093 / 1.02 / 0.92 / 1.05 / 1.21 / 1.09 / 0.96
PM01 / B08 / D-Xylose / C00181 / 0.84 / 0.66 / 0.90 / 1.31 / 2.01 / 1.83
PM01 / B09 / L-Lactic acid / C00186 / 1.49 / 1.95 / 1.70 / 1.92 / 1.30 / 2.01
PM01 / B10 / Formic acid / C00058 / 0.71 / 0.17 / 0.70 / 0.16 / 0.27 / 0.10
PM01 / B11 / D-Mannitol / C00392 / 1.38 / 1.59 / 1.49 / 1.48 / 1.46 / 1.45
PM01 / B12 / L-Glutamic acid / C00025 / 1.43 / 2.22 / 1.56 / 1.73 / 1.82 / 2.90
PM01 / C01 / D-Glucose-6-Phosphate / C00092 / 1.22 / 1.88 / 1.57 / 1.28 / 0.98 / 1.62
PM01 / C02 / D-Galactonic acid-g-Lactone / C03383 / 1.87 / 2.95 / 2.61 / 3.18 / 2.31 / 3.02
PM01 / C03 / DL-Malic acid / C00497 / 2.23 / 2.72 / 2.28 / 1.68 / 2.35 / 2.37
PM01 / C04 / D-Ribose / C00121 / 0.50 / 0.28 / 0.44 / 0.23 / 1.38 / 0.30
PM01 / C05 / Tween 20 / C11624 / 1.00 / 0.96 / 0.94 / 0.78 / 1.34 / 1.06
PM01 / C06 / L-Rhamnose / C00507 / 0.30 / 0.16 / 0.25 / 0.13 / 0.13 / 0.11
PM01 / C07 / D-Fructose / C00095 / 1.36 / 1.47 / 1.58 / 1.46 / 1.39 / 1.28
PM01 / C08 / Acetic acid / C00033 / 1.05 / 0.86 / 1.09 / 0.82 / 1.67 / 1.29
PM01 / C09 / a-D-Glucose / C00267 / 1.40 / 1.71 / 1.41 / 1.58 / 1.68 / 1.64
PM01 / C10 / Maltose / C00208 / 0.36 / 0.18 / 0.32 / 0.19 / 0.19 / 0.14
PM01 / C11 / D-Melibiose / C05402 / 0.36 / 0.19 / 0.29 / 0.19 / 0.15 / 0.12
PM01 / C12 / Thymidine / C00214 / 0.57 / 0.33 / 0.51 / 0.27 / 0.84 / 0.34
PM01 / D01 / L-Asparagine / C00152 / 1.30 / 1.29 / 1.26 / 1.29 / 1.31 / 1.35
PM01 / D02 / D-Aspartic acid / C00402 / 0.39 / 0.28 / 0.35 / 0.22 / 0.12 / 0.13
PM01 / D03 / D-Glucosaminic acid / C03752 / 1.21 / 0.70 / 1.17 / 0.52 / 1.68 / 1.26
PM01 / D04 / 1,2-Propanediol / C00583 / 0.30 / 0.18 / 0.25 / 0.18 / 0.16 / 0.13
PM01 / D05 / Tween 40 / 1.05 / 0.82 / 0.68 / 1.22 / 1.87 / 1.59
PM01 / D06 / a-Ketoglutaric acid / C00026 / 0.64 / 0.37 / 0.58 / 0.35 / 1.29 / 0.54
PM01 / D07 / a-Ketobutyric acid / C00109 / 0.82 / 0.63 / 1.16 / 0.63 / 1.57 / 1.42
PM01 / D08 / a-Methyl-D-Galactoside / 0.42 / 0.17 / 0.25 / 0.19 / 0.14 / 0.13
PM01 / D09 / a-D-Lactose / C00243 / 0.36 / 0.21 / 0.30 / 0.19 / 0.19 / 0.16
PM01 / D10 / Lactulose / C07064 / 0.42 / 0.18 / 0.36 / 0.21 / 0.64 / 0.24
PM01 / D11 / Sucrose / C00089 / 1.50 / 1.50 / 1.56 / 1.70 / 1.60 / 2.29
PM01 / D12 / Uridine / C00299 / 0.50 / 0.22 / 0.37 / 0.24 / 0.25 / 0.18
PM01 / E01 / L-Glutamine / C00064 / 1.44 / 1.64 / 1.51 / 1.50 / 1.60 / 1.84
PM01 / E02 / m-Tartaric acid / C00552 / 0.50 / 0.32 / 0.46 / 0.26 / 0.67 / 0.26
PM01 / E03 / D-Glucose-1-Phosphate / C00103 / 0.79 / 0.59 / 0.74 / 0.54 / 1.74 / 1.54
PM01 / E04 / D-Fructose-6-Phosphate / C00085 / 1.35 / 0.89 / 1.56 / 1.24 / 3.05 / 1.86
PM01 / E05 / Tween 80 / C11625 / 1.04 / 0.83 / 1.12 / 0.79 / 2.31 / 1.41
PM01 / E06 / a-Hydroxyglutaric acid-g-Lactone / 0.78 / 0.97 / 1.06 / 0.65 / 0.55 / 0.48
PM01 / E07 / a-Hydroxybutyric acid / C05984 / 0.66 / 0.58 / 0.91 / 0.58 / 1.75 / 1.57
PM01 / E08 / b-Methyl-D-Glucoside / 0.30 / 0.19 / 0.28 / 0.18 / 0.17 / 0.13
PM01 / E09 / Adonitol / C00474 / 1.19 / 1.81 / 1.55 / 1.58 / 2.69 / 1.14
PM01 / E10 / Maltotriose / C01835 / 0.38 / 0.18 / 0.35 / 0.19 / 0.17 / 0.13
PM01 / E11 / 2`-Deoxyadenosine / C00559 / 0.50 / 0.24 / 0.38 / 0.23 / 0.60 / 0.18
PM01 / E12 / Adenosine / C00212 / 0.35 / 0.18 / 0.31 / 0.18 / 0.17 / 0.14
PM01 / F01 / Gly-Asp / C02871 / 0.48 / 0.28 / 0.42 / 0.24 / 0.33 / 0.21
PM01 / F02 / Citric acid / C00158 / 1.31 / 1.25 / 1.32 / 1.79 / 1.54 / 1.23
PM01 / F03 / m-Inositol / C00137 / 1.16 / 1.40 / 1.26 / 1.20 / 2.27 / 2.66
PM01 / F04 / D-Threonine / C00820 / 0.44 / 0.26 / 0.39 / 0.23 / 0.19 / 0.17
PM01 / F05 / Fumaric acid / C00122 / 1.33 / 2.00 / 1.60 / 1.67 / 0.87 / 1.89
PM01 / F06 / Bromosuccinic acid / 0.71 / 0.65 / 0.79 / 0.58 / 0.42 / 0.50
PM01 / F07 / Propionic acid / C00163 / 1.84 / 1.49 / 1.82 / 1.39 / 1.86 / 1.96
PM01 / F08 / Mucic acid / C01807 / 1.62 / 2.77 / 1.63 / 1.93 / 2.23 / 2.17
PM01 / F09 / Glycolic acid / C00160 / 0.73 / 0.47 / 0.86 / 0.48 / 1.77 / 1.06
PM01 / F10 / Glyoxylic acid / C00048 / 0.38 / 0.23 / 0.34 / 0.20 / 0.17 / 0.16
PM01 / F11 / D-Cellobiose / C00185 / 0.39 / 0.21 / 0.32 / 0.20 / 0.17 / 0.14
PM01 / F12 / Inosine / C00294 / 0.50 / 0.22 / 0.37 / 0.25 / 0.96 / 0.21
PM01 / G01 / Gly-Glu / 0.66 / 0.35 / 0.55 / 0.25 / 0.44 / 0.25
PM01 / G02 / Tricarballylic acid / 2.30 / 2.51 / 1.89 / 2.17 / 2.09 / 2.43
PM01 / G03 / L-Serine / C00065 / 1.36 / 1.14 / 1.38 / 1.60 / 2.42 / 2.25
PM01 / G04 / L-Threonine / C00188 / 0.70 / 0.42 / 1.01 / 0.39 / 1.95 / 2.27
PM01 / G05 / L-Alanine / C00041 / 1.36 / 1.55 / 1.59 / 1.52 / 2.63 / 2.19
PM01 / G06 / Ala-Gly / 0.91 / 0.47 / 0.88 / 0.53 / 2.99 / 1.56
PM01 / G07 / Acetoacetic acid / C00164 / 0.57 / 0.35 / 0.52 / 0.35 / 0.21 / 0.18
PM01 / G08 / N-Acetyl-D-Mannosamine / C00645 / 0.33 / 0.19 / 0.29 / 0.18 / 0.12 / 0.12
PM01 / G09 / Mono-Methylsuccinate / 0.69 / 0.40 / 0.77 / 0.46 / 0.70 / 0.30
PM01 / G10 / Methylpyruvate / 1.26 / 1.40 / 1.50 / 0.94 / 2.35 / 0.66
PM01 / G11 / D-Malic acid / C00497 / 1.03 / 0.48 / 1.20 / 0.43 / 2.23 / 1.60
PM01 / G12 / L-Malic acid / C00149 / 1.51 / 2.62 / 1.63 / 1.90 / 2.52 / 2.69
PM01 / H01 / Gly-Pro / 1.43 / 1.64 / 1.68 / 1.49 / 2.77 / 2.35
PM01 / H02 / p-Hydroxyphenyl Acetic acid / C00642 / 1.41 / 1.54 / 2.05 / 1.68 / 2.00 / 1.72
PM01 / H03 / m-Hydroxyphenyl Acetic acid / C05593 / 1.45 / 1.29 / 2.15 / 1.91 / 2.08 / 2.34
PM01 / H04 / Tyramine / C00483 / 0.51 / 0.40 / 0.50 / 0.33 / 1.96 / 0.89
PM01 / H05 / D-Psicose / C06468 / 0.33 / 0.21 / 0.29 / 0.19 / 0.17 / 0.17
PM01 / H06 / L-Lyxose / C01508 / 0.17 / 0.13 / 0.18 / 0.12 / 0.12 / 0.12
PM01 / H07 / Glucuronamide / C13295 / 0.36 / 0.22 / 0.38 / 0.21 / 0.19 / 0.16
PM01 / H08 / Pyruvic acid / C00022 / 1.84 / 1.65 / 1.92 / 1.57 / 1.74 / 1.71
PM01 / H09 / L-Galactonic acid-g-Lactone / C01115 / 0.21 / 0.15 / 0.21 / 0.13 / 0.12 / 0.12
PM01 / H10 / D-Galacturonic acid / C00333 / 1.10 / 0.79 / 1.14 / 0.66 / 2.22 / 2.44
PM01 / H11 / Phenylethylamine / C05332 / 1.08 / 0.77 / 0.69 / 0.49 / 1.08 / 0.59
PM01 / H12 / 2-Aminoethanol / C00189 / 0.66 / 0.55 / 0.70 / 0.49 / 1.43 / 1.14
Carbon source / K56-2 1st run / K56-2 2nd run / Low oxygen condition
Plate / Location / Chemical / KEGG ID / OD WT / OD mutant / OD WT / OD mutant / OD WT / OD mutant
PM02A / A01 / Negative Control / 0.40 / 0.20 / 0.23 / 0.17 / 0.15 / 0.15
PM02A / A02 / Chondroitin Sulfate C / C00635 / 0.24 / 0.15 / 0.21 / 0.14 / 0.11 / 0.11
PM02A / A03 / a-Cyclodextrin / 0.32 / 0.19 / 0.22 / 0.14 / 0.17 / 0.14
PM02A / A04 / b-Cyclodextrin / 0.29 / 0.15 / 0.20 / 0.13 / 0.14 / 0.11
PM02A / A05 / g-Cyclodextrin / 0.31 / 0.20 / 0.24 / 0.16 / 0.21 / 0.12
PM02A / A06 / Dextrin / C00721 / 0.75 / 0.62 / 0.56 / 0.29 / 0.21 / 0.21
PM02A / A07 / Gelatin / C01498 / 0.29 / 0.24 / 0.25 / 0.18 / 0.15 / 0.15
PM02A / A08 / Glycogen / C00182 / 0.33 / 0.19 / 0.30 / 0.17 / 0.18 / 0.14
PM02A / A09 / Inulin / C03323 / 0.34 / 0.21 / 0.26 / 0.15 / 0.12 / 0.12
PM02A / A10 / Laminarin / C00771 / 0.29 / 0.18 / 0.24 / 0.14 / 0.14 / 0.13
PM02A / A11 / Mannan / C00464 / 0.33 / 0.20 / 0.29 / 0.16 / 0.13 / 0.12
PM02A / A12 / Pectin / C00714 / 0.37 / 0.33 / 0.35 / 0.25 / 0.46 / 0.25
PM02A / B01 / N-Acetyl-D-Galactosamine / C01132 / 0.47 / 0.24 / 0.27 / 0.20 / 0.37 / 0.19
PM02A / B02 / N-Acetyl-Neuraminic acid / C00270 / 0.34 / 0.22 / 0.27 / 0.18 / 0.12 / 0.12
PM02A / B03 / b-D-Allose / C01487 / 0.30 / 0.20 / 0.22 / 0.14 / 0.14 / 0.12
PM02A / B04 / Amygdalin / C08325 / 0.36 / 0.19 / 0.27 / 0.17 / 0.17 / 0.14
PM02A / B05 / D-Arabinose / C00216 / 1.90 / 1.30 / 1.25 / 1.37 / 1.43 / 1.46
PM02A / B06 / D-Arabitol / C01904 / 1.09 / 1.12 / 1.27 / 1.15 / 1.28 / 1.28
PM02A / B07 / pyruvate / C00532 / 0.91 / 0.65 / 1.10 / 0.92 / 1.47 / 0.52
PM02A / B08 / Arbutin / C06186 / 0.76 / 0.50 / 0.76 / 0.49 / 1.42 / 1.35
PM02A / B09 / 2-Deoxy-D-Ribose / C01801 / 0.81 / 0.32 / 0.91 / 0.29 / 2.46 / 1.08
PM02A / B10 / i-Erythritol / C00503 / 0.31 / 0.19 / 0.26 / 0.15 / 0.16 / 0.13
PM02A / B11 / D-Fucose / C01018 / 0.46 / 0.27 / 0.34 / 0.27 / 0.33 / 0.21
PM02A / B12 / 3-O-b-D-Galactopyranosyl-D-Arabinose / 0.62 / 0.36 / 0.64 / 0.32 / 0.44 / 0.19
PM02A / C01 / Gentiobiose / C08240 / 0.38 / 0.20 / 0.26 / 0.15 / 0.16 / 0.12
PM02A / C02 / L-Glucose / 0.31 / 0.17 / 0.22 / 0.14 / 0.14 / 0.12
PM02A / C03 / D-Lactitol / 0.36 / 0.24 / 0.26 / 0.18 / 0.63 / 0.22
PM02A / C04 / D-Melezitose / C08243 / 0.34 / 0.22 / 0.29 / 0.19 / 0.15 / 0.13
PM02A / C05 / Maltitol / G00275 / 0.31 / 0.22 / 0.25 / 0.16 / 0.16 / 0.12
PM02A / C06 / a-Methyl-D-Glucoside / 0.38 / 0.22 / 0.24 / 0.16 / 0.19 / 0.13
PM02A / C07 / b-Methyl-D-Galactoside / C03619 / 0.74 / 0.43 / 0.79 / 0.52 / 1.48 / 1.13
PM02A / C08 / 3-Methylglucose / 0.33 / 0.21 / 0.27 / 0.21 / 0.19 / 0.15
PM02A / C09 / b-Methyl-D-Glucuronic acid / C08350 / 0.31 / 0.21 / 0.25 / 0.17 / 0.12 / 0.12
PM02A / C10 / a-Methyl-D-Mannoside / 0.37 / 0.25 / 0.24 / 0.17 / 0.12 / 0.13
PM02A / C11 / b-Methyl-D-Xyloside / 0.37 / 0.25 / 0.22 / 0.20 / 0.12 / 0.13
PM02A / C12 / Palatinose / C01742 / 0.30 / 0.23 / 0.24 / 0.16 / 0.12 / 0.12
PM02A / D01 / D-Raffinose / C00492 / 0.39 / 0.21 / 0.25 / 0.17 / 0.21 / 0.16
PM02A / D02 / Salicin / C01451 / 0.68 / 0.27 / 0.55 / 0.20 / 1.38 / 0.38
PM02A / D03 / Sedoheptulosan / 0.44 / 0.18 / 0.24 / 0.17 / 0.18 / 0.14
PM02A / D04 / L-Sorbose / C00247 / 0.33 / 0.22 / 0.27 / 0.18 / 0.17 / 0.13
PM02A / D05 / Stachyose / C01613 / 0.34 / 0.23 / 0.31 / 0.17 / 0.13 / 0.12
PM02A / D06 / D-Tagatose / C00795 / 0.50 / 0.32 / 0.59 / 0.30 / 0.34 / 0.32
PM02A / D07 / Turanose / G03588 / 0.40 / 0.28 / 0.32 / 0.22 / 0.21 / 0.17
PM02A / D08 / Xylitol / C00379 / 0.93 / 0.87 / 1.11 / 0.91 / 1.34 / 1.06
PM02A / D09 / N-Acetyl-D-Glucosaminitol / 0.38 / 0.24 / 0.31 / 0.20 / 0.13 / 0.11
PM02A / D10 / g-Amino-N-Butyric acid / C00334 / 0.89 / 0.69 / 0.85 / 0.58 / 1.10 / 1.24
PM02A / D11 / d-Amino Valeric acid / 0.30 / 0.21 / 0.22 / 0.16 / 1.72 / 0.24
PM02A / D12 / Butyric acid / C00246 / 1.45 / 1.12 / 1.33 / 1.28 / 2.82 / 1.72
PM02A / E01 / Capric acid / C01571 / 0.46 / 0.38 / 0.43 / 0.64 / 0.63 / 0.96
PM02A / E02 / Caproic acid / C01585 / 1.22 / 1.30 / 1.23 / 1.57 / 1.72 / 1.52
PM02A / E03 / Citraconic acid / C02226 / 1.99 / 1.71 / 1.71 / 2.29 / 1.36 / 2.26
PM02A / E04 / Citramalic acid / C00815 / 1.02 / 0.77 / 1.05 / 0.68 / 1.34 / 0.85
PM02A / E05 / D-Glucosamine / C00329 / 1.78 / 1.37 / 1.97 / 1.60 / 2.54 / 1.60
PM02A / E06 / 2-Hydroxybenzoic acid / C00805 / 0.09 / 0.10 / 0.10 / 0.10 / 0.10 / 0.09
PM02A / E07 / 4-Hydroxybenzoic acid / C00156 / 1.22 / 1.19 / 1.58 / 1.31 / 2.09 / 1.96
PM02A / E08 / b-Hydroxybutyric acid / C01089 / 2.05 / 1.75 / 1.56 / 2.21 / 0.97 / 1.99
PM02A / E09 / g-Hydroxybutyric acid / C00989 / 0.34 / 0.20 / 0.29 / 0.20 / 0.20 / 0.15
PM02A / E10 / a-Keto-Valeric acid / C00567 / 0.45 / 0.30 / 0.43 / 0.22 / 0.13 / 0.12
PM02A / E11 / Itaconic acid / C00490 / 0.26 / 0.26 / 0.30 / 0.24 / 0.47 / 0.28
PM02A / E12 / 5-Keto-D-Gluconic acid / C01062 / 0.50 / 0.32 / 0.44 / 0.25 / 0.69 / 0.28
PM02A / F01 / D-Lactic acid Methyl Ester / 1.00 / 0.68 / 0.81 / 0.69 / 0.65 / 0.93
PM02A / F02 / Malonic acid / C00383 / 1.23 / 1.08 / 1.26 / 0.95 / 1.81 / 1.29
PM02A / F03 / Melibionic acid / 0.42 / 0.21 / 0.30 / 0.18 / 0.14 / 0.16
PM02A / F04 / Oxalic acid / C00209 / 0.31 / 0.19 / 0.24 / 0.17 / 0.12 / 0.12
PM02A / F05 / Oxalomalic acid / C01990 / 0.51 / 0.35 / 0.38 / 0.27 / 0.16 / 0.15
PM02A / F06 / Quinic acid / C00296 / 0.37 / 2.13 / 0.27 / 2.52 / 0.16 / 3.08
PM02A / F07 / D-Ribono-1,4-Lactone / 0.23 / 0.16 / 0.20 / 0.14 / 0.23 / 0.16
PM02A / F08 / Sebacic acid / C08277 / 1.24 / 1.29 / 1.32 / 1.61 / 1.37 / 1.40
PM02A / F09 / Sorbic acid / 0.58 / 0.47 / 0.50 / 0.40 / 0.59 / 0.44
PM02A / F10 / Succinamic acid / 0.50 / 0.38 / 0.48 / 0.36 / 1.06 / 0.52
PM02A / F11 / D-Tartaric acid / C02107 / 0.42 / 0.26 / 0.36 / 0.22 / 0.15 / 0.15
PM02A / F12 / L-Tartaric acid / C00898 / 1.13 / 0.88 / 1.16 / 0.91 / 1.20 / 1.63
PM02A / G01 / Acetamide / C06244 / 0.50 / 0.35 / 0.58 / 0.46 / 0.54 / 0.34
PM02A / G02 / L-Alaninamide / 0.63 / 0.31 / 0.46 / 0.30 / 0.22 / 0.20
PM02A / G03 / N-Acetyl-L-Glutamic acid / C00624 / 0.38 / 0.22 / 0.32 / 0.17 / 0.15 / 0.15
PM02A / G04 / L-Arginine / C00062 / 1.60 / 1.81 / 1.88 / 1.93 / 2.38 / 2.84
PM02A / G05 / Glycine / C00037 / 0.52 / 0.37 / 0.63 / 0.31 / 1.02 / 0.51
PM02A / G06 / L-Histidine / C00135 / 1.26 / 1.41 / 1.20 / 1.18 / 1.45 / 1.69
PM02A / G07 / L-Homoserine / C00263 / 0.15 / 0.14 / 0.14 / 0.13 / 0.12 / 0.11
PM02A / G08 / Hydroxy-L-Proline / C01015 / 1.28 / 1.43 / 1.45 / 1.36 / 1.57 / 1.41
PM02A / G09 / L-Isoleucine / C00407 / 0.51 / 0.18 / 0.54 / 0.17 / 1.12 / 0.29
PM02A / G10 / L-Leucine / C00123 / 0.55 / 0.24 / 0.57 / 0.18 / 0.70 / 0.31
PM02A / G11 / L-Lysine / C00047 / 0.73 / 0.70 / 1.09 / 1.05 / 1.46 / 1.57
PM02A / G12 / L-Methionine / C00073 / 0.30 / 0.17 / 0.20 / 0.14 / 0.13 / 0.12
PM02A / H01 / L-Ornithine / C00077 / 0.63 / 0.31 / 0.56 / 0.25 / 0.94 / 0.32
PM02A / H02 / L-Phenylalanine / C00079 / 1.32 / 1.11 / 1.29 / 1.15 / 1.82 / 2.03
PM02A / H03 / L-Pyroglutamic acid / C02238 / 1.63 / 1.68 / 1.51 / 2.02 / 2.18 / 2.60
PM02A / H04 / L-Valine / C00183 / 0.53 / 0.28 / 0.48 / 0.19 / 0.68 / 0.29
PM02A / H05 / D,L-Carnitine / C00487 / 0.33 / 0.21 / 0.29 / 0.19 / 0.14 / 0.13
PM02A / H06 / sec-Butylamine / 0.59 / 0.33 / 0.36 / 0.28 / 0.28 / 0.19
PM02A / H07 / D,L-Octopamine / C04227 / 1.04 / 0.97 / 1.16 / 1.36 / 2.20 / 1.26
PM02A / H08 / Putrescine / C00134 / 0.78 / 0.42 / 0.78 / 0.23 / 0.57 / 0.48
PM02A / H09 / Dihydroxyacetone / C00184 / 0.41 / 0.22 / 0.34 / 0.20 / 0.33 / 0.21
PM02A / H10 / 2,3-Butanediol / C03044 / 0.63 / 0.44 / 0.57 / 0.41 / 0.42 / 0.40
PM02A / H11 / 2,3-Butanedione / C00741 / 0.33 / 0.16 / 0.16 / 0.16 / 0.15 / 0.12
PM02A / H12 / 3-Hydroxy-2-butanone / C00466 / 0.51 / 0.21 / 0.27 / 0.18 / 0.26 / 0.18
Nitrogen source / K56-2 1st run / K56-2 2nd run / Low oxygen condition
Plate / Location / Chemical / KEGG ID / OD WT / OD mutant / OD WT / OD mutant / OD WT / OD mutant
PM03B / A01 / Negative Control / 0.35 / 0.29 / 0.34 / 0.35 / 0.26 / 0.24
PM03B / A02 / Ammonia / C00014 / 1.22 / 0.97 / 1.26 / 1.00 / 1.18 / 1.16
PM03B / A03 / Nitrite / C00088 / 0.76 / 0.60 / 0.88 / 0.74 / 0.97 / 0.79
PM03B / A04 / Nitrate / C00244 / 1.14 / 0.28 / 1.16 / 0.31 / 1.29 / 0.23
PM03B / A05 / Urea / C00086 / 1.24 / 1.24 / 1.33 / 1.24 / 1.24 / 1.24
PM03B / A06 / Biuret / C06555 / 0.31 / 0.26 / 0.33 / 0.33 / 0.29 / 0.23
PM03B / A07 / L-Alanine / C00041 / 1.10 / 1.06 / 1.19 / 1.02 / 1.22 / 1.19
PM03B / A08 / L-Arginine / C00062 / 1.19 / 1.27 / 1.14 / 1.05 / 1.21 / 1.19
PM03B / A09 / L-Asparagine / C00152 / 1.26 / 1.13 / 1.22 / 1.19 / 1.18 / 1.20
PM03B / A10 / L-Aspartic acid / C00049 / 1.29 / 1.21 / 1.21 / 1.17 / 1.19 / 1.23
PM03B / A11 / L-Cysteine / C00097 / 1.16 / 1.09 / 1.11 / 1.21 / 1.19 / 1.23
PM03B / A12 / L-Glutamic acid / C00025 / 1.22 / 1.28 / 1.25 / 1.11 / 1.17 / 1.13
PM03B / B01 / L-Glutamine / C00064 / 1.35 / 1.38 / 1.27 / 1.18 / 1.26 / 1.26
PM03B / B02 / Glycine / C00037 / 1.35 / 1.28 / 1.28 / 1.19 / 1.29 / 1.16
PM03B / B03 / L-Histidine / C00135 / 1.24 / 1.21 / 1.19 / 1.12 / 1.20 / 1.23
PM03B / B04 / L-Isoleucine / C00407 / 0.43 / 0.44 / 0.43 / 0.44 / 0.57 / 0.41
PM03B / B05 / L-Leucine / C00123 / 0.61 / 0.57 / 0.63 / 0.69 / 0.66 / 0.43
PM03B / B06 / L-Lysine / C00047 / 0.84 / 0.75 / 0.97 / 0.97 / 1.06 / 1.16
PM03B / B07 / L-Methionine / C00073 / 0.27 / 0.25 / 0.36 / 0.31 / 0.26 / 0.27
PM03B / B08 / L-Phenylalanine / C00079 / 0.75 / 0.68 / 0.80 / 0.64 / 0.85 / 0.83
PM03B / B09 / L-Proline / C00148 / 1.09 / 1.06 / 0.92 / 1.00 / 1.18 / 1.22
PM03B / B10 / L-Serine / C00065 / 1.27 / 1.21 / 1.14 / 1.08 / 1.23 / 1.23
PM03B / B11 / L-Threonine / C00188 / 1.07 / 0.85 / 1.09 / 1.03 / 1.17 / 1.12
PM03B / B12 / L-Tryptophan / C00078 / 0.96 / 0.80 / 1.04 / 0.91 / 1.16 / 0.99
PM03B / C01 / L-Tyrosine / C00082 / 1.06 / 0.85 / 1.10 / 1.02 / 1.16 / 1.19
PM03B / C02 / L-Valine / C00183 / 0.56 / 0.45 / 0.47 / 0.63 / 0.39 / 0.33
PM03B / C03 / D-Alanine / C00133 / 0.95 / 1.00 / 0.99 / 1.02 / 1.12 / 1.17
PM03B / C04 / D-Asparagine / C01905 / 1.20 / 1.16 / 1.33 / 1.21 / 1.15 / 1.20
PM03B / C05 / D-Aspartic acid / C00402 / 0.82 / 0.91 / 1.09 / 0.98 / 0.97 / 0.87
PM03B / C06 / D-Glutamic acid / C00217 / 0.79 / 0.74 / 0.94 / 0.86 / 0.98 / 0.89
PM03B / C07 / D-Lysine / C00739 / 0.99 / 1.01 / 1.04 / 1.11 / 1.31 / 1.33
PM03B / C08 / D-Serine / C00740 / 0.95 / 0.85 / 1.02 / 0.87 / 1.11 / 1.29
PM03B / C09 / D-Valine / C06417 / 0.57 / 0.47 / 0.60 / 0.57 / 0.88 / 0.62
PM03B / C10 / L-Citrulline / C00327 / 0.86 / 1.04 / 0.94 / 1.01 / 1.55 / 1.41
PM03B / C11 / L-Homoserine / C00263 / 0.52 / 0.24 / 0.62 / 0.33 / 0.52 / 0.46
PM03B / C12 / L-Ornithine / C00077 / 0.88 / 0.76 / 1.02 / 0.97 / 1.10 / 1.17
PM03B / D01 / N-Acetyl-L-Glutamic acid / C00624 / 0.64 / 0.53 / 0.86 / 0.63 / 0.69 / 0.54
PM03B / D02 / N-Phthaloyl-L-Glutamic acid / 0.28 / 0.24 / 0.33 / 0.29 / 0.32 / 0.22
PM03B / D03 / L-Pyroglutamic acid / C02238 / 1.10 / 1.08 / 1.20 / 1.19 / 1.18 / 1.26
PM03B / D04 / Hydroxylamine / C00192 / 0.09 / 0.09 / 0.08 / 0.08 / 0.07 / 0.07
PM03B / D05 / Methylamine / C00218 / 0.28 / 0.35 / 0.42 / 0.42 / 0.29 / 0.19
PM03B / D06 / N-Amylamine / 0.70 / 0.77 / 0.70 / 0.87 / 1.25 / 1.29
PM03B / D07 / N-Butylamine / 0.37 / 0.40 / 0.45 / 0.46 / 0.30 / 0.33
PM03B / D08 / Ethylamine / C00797 / 0.34 / 0.24 / 0.41 / 0.32 / 0.31 / 0.26
PM03B / D09 / Ethanolamine / C00189 / 1.08 / 1.10 / 1.17 / 1.04 / 1.18 / 1.36
PM03B / D10 / Ethylenediamine / C12511 / 0.18 / 0.13 / 0.23 / 0.15 / 0.13 / 0.11
PM03B / D11 / Putrescine / C00134 / 2.26 / 1.59 / 1.83 / 2.68 / 2.63 / 2.63
PM03B / D12 / Agmatine / C00179 / 2.50 / 2.78 / 2.67 / 2.75 / 2.72 / 2.32
PM03B / E01 / Histamine / C00388 / 1.81 / 1.84 / 1.97 / 1.46 / 1.74 / 1.11
PM03B / E02 / b-Phenylethylamine / C05332 / 1.04 / 0.93 / 1.07 / 1.11 / 1.23 / 1.25
PM03B / E03 / Tyramine / C00483 / 0.97 / 0.98 / 1.00 / 0.99 / 1.22 / 1.12
PM03B / E04 / Acetamide / C06244 / 0.73 / 0.62 / 1.25 / 1.13 / 1.30 / 1.20
PM03B / E05 / Formamide / C00488 / 0.48 / 0.41 / 0.81 / 0.62 / 0.39 / 0.32
PM03B / E06 / Glucuronamide / C13295 / 0.71 / 0.57 / 0.79 / 0.63 / 0.48 / 0.46
PM03B / E07 / DL-Lactamide / 0.33 / 0.35 / 0.52 / 0.47 / 0.51 / 0.39
PM03B / E08 / D-Glucosamine / C00329 / 0.36 / 0.22 / 0.39 / 0.21 / 0.26 / 0.22
PM03B / E09 / D-Galactosamine / C02262 / 0.24 / 0.22 / 0.33 / 0.24 / 0.28 / 0.22
PM03B / E10 / D-Mannosamine / C03570 / 0.40 / 0.25 / 0.46 / 0.34 / 0.24 / 0.40
PM03B / E11 / N-Acetyl-D-Glucosamine / C00140 / 0.25 / 0.23 / 0.31 / 0.24 / 0.19 / 0.21
PM03B / E12 / N-Acetyl-D-Galactosamine / C01132 / 0.25 / 0.24 / 0.27 / 0.23 / 0.22 / 0.26
PM03B / F01 / N-Acetyl-D-Mannosamine / C00645 / 0.24 / 0.21 / 0.26 / 0.23 / 0.26 / 0.25
PM03B / F02 / Adenine / C00147 / 1.22 / 1.18 / 1.37 / 1.08 / 1.23 / 1.15
PM03B / F03 / Adenosine / C00212 / 1.02 / 0.26 / 0.98 / 0.48 / 1.33 / 0.78
PM03B / F04 / Cytidine / C00475 / 0.43 / 0.37 / 0.49 / 0.52 / 0.80 / 0.68
PM03B / F05 / Cytosine / C00380 / 0.87 / 0.85 / 1.09 / 1.03 / 1.05 / 1.20
PM03B / F06 / Guanine / C00242 / 1.45 / 0.99 / 1.38 / 0.86 / 1.53 / 0.47
PM03B / F07 / Guanosine / C00387 / 0.87 / 0.56 / 1.15 / 0.64 / 1.31 / 0.60
PM03B / F08 / Thymine / C00178 / 0.69 / 0.68 / 0.83 / 0.74 / 1.05 / 0.91
PM03B / F09 / Thymidine / C00214 / 0.70 / 0.85 / 0.80 / 0.86 / 1.13 / 1.19
PM03B / F10 / Uracil / C00106 / 0.88 / 0.82 / 0.98 / 0.87 / 1.06 / 1.14
PM03B / F11 / Uridine / C00299 / 0.58 / 0.30 / 0.62 / 0.49 / 0.80 / 0.38
PM03B / F12 / Inosine / C00294 / 1.00 / 0.23 / 1.05 / 0.32 / 1.20 / 0.22
PM03B / G01 / Xanthine / C00385 / 1.26 / 0.59 / 1.35 / 0.70 / 1.11 / 0.49
PM03B / G02 / Xanthosine / C01762 / 0.33 / 0.23 / 0.45 / 0.23 / 0.45 / 0.22
PM03B / G03 / Uric acid / C00366 / 0.88 / 0.73 / 1.32 / 0.53 / 1.32 / 0.83
PM03B / G04 / Alloxan / C07599 / 0.16 / 0.15 / 0.18 / 0.15 / 0.16 / 0.13
PM03B / G05 / Allantoin / C01551 / 1.22 / 1.25 / 1.26 / 1.16 / 1.28 / 1.21
PM03B / G06 / Parabanic acid / 0.28 / 0.28 / 0.34 / 0.34 / 0.33 / 0.26
PM03B / G07 / DL-a-Amino-N-Butyric acid / C02261 / 0.72 / 0.69 / 0.91 / 0.68 / 1.10 / 0.83
PM03B / G08 / g-Amino-N-Butyric acid / C00334 / 1.23 / 1.03 / 1.23 / 1.13 / 1.23 / 1.27
PM03B / G09 / e-Amino-N-Caproic acid / C02378 / 0.30 / 0.26 / 0.29 / 0.32 / 0.31 / 0.30
PM03B / G10 / DL-a-Amino-Caprylic acid / 0.08 / 0.10 / 0.14 / 0.08 / 1.01 / 0.31
PM03B / G11 / d-Amino-N-Valeric acid / C00431 / 1.00 / 0.81 / 1.16 / 0.91 / 1.02 / 1.05
PM03B / G12 / a-Amino-N-Valeric acid / C01826 / 0.56 / 0.42 / 0.46 / 0.41 / 0.30 / 0.22
PM03B / H01 / Ala-Asp / C02871 / 1.08 / 0.99 / 1.21 / 1.11 / 1.26 / 1.23
PM03B / H02 / Ala-Gln / 1.24 / 1.12 / 1.21 / 1.16 / 1.35 / 1.23
PM03B / H03 / Ala-Glu / 1.07 / 1.03 / 1.11 / 1.11 / 1.19 / 1.16
PM03B / H04 / Ala-Gly / 1.08 / 0.95 / 1.05 / 1.10 / 1.22 / 1.10
PM03B / H05 / Ala-His / 1.17 / 1.22 / 1.21 / 1.21 / 1.17 / 1.14
PM03B / H06 / Ala-Leu / C05035 / 1.14 / 0.99 / 1.22 / 1.03 / 1.18 / 1.13
PM03B / H07 / Ala-Thr / 1.01 / 1.06 / 1.22 / 1.02 / 1.21 / 1.16
PM03B / H08 / Gly-Asn / 1.13 / 1.17 / 1.21 / 1.17 / 1.15 / 1.21
PM03B / H09 / Gly-Gln / 1.24 / 1.21 / 1.15 / 1.11 / 1.22 / 1.33
PM03B / H10 / Gly-Glu / 1.10 / 1.19 / 1.18 / 1.14 / 1.30 / 1.34
PM03B / H11 / Gly-Met / 1.06 / 0.99 / 1.04 / 1.00 / 1.21 / 1.12
PM03B / H12 / Met-Ala / 1.08 / 1.07 / 1.21 / 0.98 / 1.39 / 1.26
1