Article title: Influence of the MHC genotype on the progression of experimental SIV infection in the Mauritius cynomolgus macaque
Journal name: Immunogenetics
Author names: Alice Aarnink, Roger Le Grand, Bruno Vaslin, Nathalie Bosquet, Pol-André Apoil, Peter Winterton, Antoine Blancher
Corresponding author: Antoine Blancher
Laboratoire d'Immunogénétique moléculaire, EA 3034, Faculté de Médecine Purpan, Université Paul Sabatier, Toulouse 3, IFR150 (INSERM), CHU de Toulouse, 1 avenue Jean Poulhes, TSA 50032, 31059 Toulouse cedex 9, France
Supplementary Table 1: Primers used to amplify the 20 MHC microsatellites (a) and 3 KIR microsatellites (b).
Legend: reference quoted in the table: (1) Wiseman et al. 2007, (2) Karl et al. 2008 (3) Bonhomme et al. 2005 and (4) Bimber et al. 2008
a.
Microsatellite / Forward / Reverse / Ref.D6S2972 / AAATGTGAGAATAAAGGAGA / GATAAAGGGGAACTACTACA / 1
D6S2970 / TCCCATGGTCAAGTTCTCAG / TCATGGATCTTATCAGCCTC / 1
D6S2854 / TCATGAGCGTGGCACTGCAC / CCGTATGTTGCAACCAGGAG / 1
D6S2704 / TTTTGCCACTCTGGAGGATGGG / GAGCATAATATCTGGTCTACTGC / 1
D6S2847 / TATTGGACAGCACTGCTCTGG / TGCCATTCAGATTGGTTTTTCTG / 1
C4-2-25 / ATGTTAGTTTTAGAAGATAACACTC / TCTTCTGTGCAAGCAAGCACTGTAC / 1
D6S2691 / GTAGCTGTGGAAACAGTGTCCATG / CTTGACTTGAAACTCAGAGACC / 1
B2 / GTAGGAGAATTGCTTGAACCCG / TTGCAAACATTAGGACCTTTCTTG / Shiina T. (personnal communication)
P03-193435 / CAGGAGTGCTGAAGTCAT / CACTCCAGAACCTGGTGACA / 2
MICA / CCTTTTTTTCAGGGAAAGTGC / CCTTACCATCTCCAGAAACTGC / 1
D6S2793 / CTACCTCCTTGCCAAACTTGCTATTTGT / AATAGCCATGAGAAGCTATGTGGGGGA / 1
D6S2782 / TTTACTTGCTCTCACTCTCAGGCC / GGAAGACATTAACTTGTTAGCA / 1
D6S2669 / TGCCTTCCGTAAGCCTCAGTCT / TTAAGGACAGCAAAGCCAGCAGCA / 1
D6S2892 / TGCATGTCCTGTGAGGTAAG / ACTCAACCCTGCTGTTGTAG / 1
DRACA / TGGAATCTCATCAAGGTCAG / ACATTTGTATGCTTCAGATG / 3
D6S2876 / GGTAAAATTCCTGACTGGCC / GACAGCTCTTCTTAACCTGC / 1
D6S2747 / AGGAATCTAGTGCTCTCTCC / CTCTAGCAAAAGGAAGAGCC / 1
D6S2745 / CCTAGAGATTCCTCCACATTA / CCAATGTTTGATAGCAGACTGGGGT / 1
D6S2771 / ATTCCTTTCACTAGTTCTGG / CCACTTTAAGAAATTAGAAAAG / 1
D6S2741 / AGACTAGATGTAGGGCTAGC / CTGCACTTGGCTATCTCAAC / 1
b.
Microsatellite / Forward / Reverse / Ref.BX1183 / GCAATGTAGAAAACATATTTGAGGA / TTAGCCTGATTGGGTTCCTT / 4
BX7240 / TGTGGAGATGGATTGCAGAG / TGACCCTACTTGGATTGGTTG / 4
8469-20138 / TAAATAGGTAGATGATAGGTAG / CAATTGTCTGTCTATATCCACCAT / 4
Supplementary Fig. 1 Influence of H1, H3 and H5 haplotypes on logPVLD100.
LogPVLD100 was compared in animals with (+) or without (-) H1 (a), H3 (b) and H5 (c) haplotypes in the region defined by the three markers “C4-2-25”-“D6S2691”-“B2”. The box-and-whisker plot shows the median values and the 10th, 25th, 75th, and 90th percentiles. The numbers (N) of animals in each group are shown under the abscissa. For each comparison, the animals inoculated by the two routes (intravenous and intrarectal), are distinguished (dark and grey circles, respectively). The p values were calculated by means of the Mann and Whitney test under the dominant effect hypothesis.