Archaeal Communities in the Sediments of Different Mangrove Stands at Dongzhaigang,China

electronic supplementary material

sediments, sec 4 • sediment-ecology interactions • research article

Archaeal communities in the sediments of different mangrove stands at Dongzhaigang,China

Wei Li1,2• Wei Guan3• Huai Chen1,2• Baowen Liao3• Ji Hu1,2• Changhui Peng4,5• Junpeng Rui1• Jianqing Tian6• Dan Zhu1,2• Yixin He1,2

Received: 7 November 2015 / Accepted: 1 April 2106

Responsible editor: Haihan Zhang

1Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China

2Zoige Peatland and Global Change Research Station, Chinese Academy of Sciences, Hongyuan 624400, China

3Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China

4Laboratory for Ecological Forecasting and Global Change, Northwest A&F University, Yangling 712100, China

5Institut des Sciences de l’Environnement, Université du Québec à Montréal (UQAM), 201 Président-Kennedy, Montréal, H2X3Y7, Canada

6Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Huai Chen

Establishing of sampling plots and methane flux measurement

We measured the methane flux monthly from January to December in 2013.In each plot, three static chambers were placed randomly to measure methane fluxes from the bare sediments. The chambers were made of 30 cm in diameter cylindrical polyvinyl chloride (PVC) pipe with a 50 cm height. Detailed information about chambers was described in our previous paper(Chen et al. 2009).Gas samples were collected from 8 to 12a.m. at 10min intervals by 10 ml evacuated tubes over a 30 min period after enclosure. For all measurements, the air temperatures inside chambers were recorded at the beginning and the end of each sampling.

The methane concentration was determined by a gas chromatography (Agilent 7890A, Agilent Co., USA), equipped with a FID (flame ionization detector) operating at350°C. The column oven temperature was 35°C and the carrier gas was N2 with a flow rate of 20 cm3 min–1. The minimum detectable concentration is 1×10-3μl–1 (ppb). Certified methane standard in 4.9μl–1 (China methane National Research Center for Certified Reference Materials, Beijing) was used for calibration.

The flux F of methane was calculated as:

where dc/dt is the rate of concentration change; P is the atmospheric pressure of the sampling site;M is the molar mass of methane; T is the absolute temperature at sampling time; V0, P0, and T0are themolar volume, atmospheric pressure, and absolute temperature, respectively, under standard conditions; V is the volume of chambers; A is the basal area for static chambers.

References

Chen H, Wu N, Gao Y, Wang Y, Luo P, Tian J (2009) Spatial variations on methane emissions from Zoige alpine wetlands of Southwest China.SciTotal Environ407:1097-1104

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