Aquaculture in the Classroom Notes – July 18, 2002

Aquaculture at Virginia Tech is now divided among three academic divisions:

–Agriculture (including Food Science and Technology Department, and Fisheries and Wildlife Sciences)

–Veterinary Medicine (Bio-Medical Sciences and Pathobiology)

–Civil and Environmental Engineering

George Flick: Fish-Tech course was oriented to Home Ec teachers many years ago.

Laura Douglas

–CEU Credits:

Eric F.: Graduate Credit: ALS 5984 (Aquaculture in the Classroom) – Lesson on Integration in the Classroom – and 1 page classroom. $435 for 3 credits (Summer 2) – October 8 (at Ag Ed Society meeting)

CFAST =

Ewin McLean

By 2030, fisheries must increase by 50% (but probably over 100%), but most fisheries are at or above maximum capacity.

Fastest Growing food sector in the world until overtaken by organic farming this year.

Production vs. Value leads to a gold rush mentality in aquaculture, which leads to environmental degradation (shrimp is still the biggest culprit)

Non-Food Aquaculture:

Ornamental plants, fish

Bait

Crustaceans

Sport/gamefish

Corals

Amphibians

Reptiles – second most valuable (crocadilians)

Holuthuria – Sea cucumber

Nudibranchs

Molluscs

Seaweed is the most important aquaculture species in both production and importance to

Production, by species (most not familiar to US):

Kelp

Silver carp

Grass carp

Common carp

Bighead carp

Yesso scallop

Pacific oyster

Tilapia

Salmonids

Photos on CD ROM

Algal products are single-most important food additives (George Flick specialty)

Used from cooling power tools to biotech substrates, toothpastes, etc. Many sell for over $2000 per pound

Squid in Galveston, TX is now important for biotech

Risk is proportional to inputs:

Financial

Water requirements and availability

Environmental damage

Theft

Animal damage

Natural disaster

Fishmeal availability/competition

Elevated management needs

Power failures

Disease

Water quality

Mechanical and engineering failure

Trout farmers in Denmark didn’t meet effluent guidelines.

PBS.org has a pearl production video

See CD-ROM

Greg Boardman – Civil and Environmental Engineering

Michael Schwartz

See CD-ROM or handouts

Recirculating = 90% of water stays in system per day.

Biosolids can consume more O2 than the fish!

Biosolids cause gill irritation in slamonids and perch

Sand filters by Jaccuzzi

B-cell and KMT are state-of-the art (replacing bio-balls)

O3 is very effective in freshwater research systems without the byproducts that occur in saltwater.

Avoid ozone in a school – use a protein skimmer and UV system instead

Anaesthesia – Dr. Steve Smith

MS-222 is the only aneathetic allowed by FDA (tricaine Methanesulfonate). It is a carcinogen and a terratogen. Only two sources: Agent Chemical and Western Chemical. 10g will cost $120.

Use a standard dose 150mg per Liter of water rather than a dose effect. Sedation happens at 5 minutes, anesthesia in 7 minutes.

MS-222 will euthenize. Freezing is inhumane. Other humane methods are CO2 or cervical separation.

You must isolate them for 21 days in a revival tank if they are a food fish.

You must buffer MS-222 with sodium Bicarboanate Na CO3 – otherwise it will drop the pH (MS-222 has a pH of 4)

Clove oil (available in pharmacies) will sedate but not anesthetize. CO is too dangerous.

Striped bass react5 quickly, tilapia are resistant.

Catfish don’t roll over.

Tags: Pit tag (abdominal scannable), operculum tag, or fillet tag.

Steve Smith and Kathleen Hughes

Get a spectrophotometer to be more accurate than test kits.

Consider gill clip technique for parasites.

Prophylactic treatment is not legal by FDA, even with salt

1-2 PPT of salt is sufficient for treating fish but it will be detrimental to plants.

Formalin will be detrimental to your bio-filter, so do it externally or bypass the filter.

Top-dress feed (chemoth

Most only approved for catfish, salmonids, and lobster. You must have a vet prescribe for tilapia, bass, and all others.

Use quarantine!

Necrospy Technique

Cervical separation – sever the spinal chord with a scissor (no flopping, either)

  1. Examine external surface
  2. Remove operculum
  3. Remove gill arch (not just filaments) – put in 10% neutral buffered formula
  4. Cut across chest in front of pelvic fins
  5. Cut toward vent, but steer away from opening

Trout – carnivore – very short digestive tract

Goldfish – herbivore – long, coiled, no stomach

Tilapia – often fed carnivore diet, but even more herbivorous than goldfish

  1. Remove viscera to expose gonads, swim bladder, and even deeper, the kidney
  2. Bacterial culture from posterior kidney with culturette or sterile pipette
  3. From original cut, remove frontal cranial cap, break through cartilage. Take sample with culturette.

Nutrition – Steve Craig

See handouts or CD-Rom

Protein - Kjeldahl method for crude protein- sulfuric acid extraction to measure nitrogen content

Fish meal supply has been flat for the last ten years and could be a limiting factor. Salmonids cannot use plant protein effectively.

Protein is a major source of nitrogen in effluents.

10 essential amino acids

We aim for minimum protein requirement that allows for maximum growth (also reduces impact of effluent).

Lipids – most efficient energy source - protein should not be used for energy.

Marine source are major source of N3 fatty acids (omega)

Fish oil supplies are also decreasing.

Carbohydates – not much work done to date – hard for some fish to use, but red drum can use up to 35% carbos

Energy – growth , maintenance and reproduction

Fish have several energy advantages:

Secrete ammonia

Do not regulate body temp.

Do not have to fight gravity

Energy:Protein ratio is the hot topic – too high energy, fish will stop feeding before sated.

Ask Steve () - Can tilapia survive on alfalfa pellets?

You should not keep a 50 lb bag over 90 days, and if kept that long you should keep it cool and dry and rodent-protected.

Hydroponics – Charlie Schultz

William James quote: “In teaching you must simply work…”

Permit required.

VTU is a source for fingerlings. Dr. Hollerman? (Genetics)

Lynn ____

Bert ____

Nile Tilapia – grown to 2 pounds for tourist (filets)

Hybrid Red Tilia – grown to 1 pound (fits on a plate)

Biomass Density Estimate

Tilapia mozambiqua – best for salt water

Tilapia aurais – colder water

Mouth brooders – held up to 2 weeks from fertilized eggs

Commercial system – best is ¼ acre or larger

Ideas for classroom:

Multiple tanks of different size fish

Water leaves tank to a clarifier to remove larger solids (fine solids benefit plants)

Tilapia sludge is a valuable fertilizer

Fine solids “mineralize”

Anaerobic zones in the

In hydroponic system you want 4-5 mg/L D.O.

Tetras (blackfin) are used under the plants and feed on zooplankton.

Snails feed on good bacteria, use a red-ear sunfish (pumpkinseed?) to eat the snails.

One pump for the whole system – ½ HP 105 GPM

To control worms, use B.t. products.

White flies, aphids use Buvera buvins (a fungus)

Do not use Neem(sp?) (toxic to fish)

See handout for educational components

1.5 pounds of food produces 1 pound of Tilapia (cows are over 13!)

Float system with “net cups” placed in insulation board.

Floating lettuce, collards, beans and melons, mint and chives (gravel might be better to allow spreading).

Nutrient film technique (NFT) – rain gutters

Flood and drain system (ebb and flow) – use pea gravel – flowers too

S&S (SNS?) system

Sand Culture

Gravel culture

Sodium thiosulfate to treat chlorine

7.0 in an aquaponic system

Add iron, calcium and potassium (by adding potassium hydroxide and calcium hydroxide)

PH pen is necessary

27oC should be max.

Feed ratios – 80g food per M2 of growing area

Grow 1 fish per ½ gallon of water but 1 fish per 2 gallons is safer

Rps.uvi.edu/AES/Aquaculture/UVIShortcourse.html

Aquaponics listserve – others too

Aquaponics journal

Hydrogardens.com

Aquaponics.com

Vermiculture – Lori Marsh

Some aquaculture facilities have to pay a surcharge because of the O2 demand on their systems.

Vermicomposting in the classroom paper is in our

Worms:

Turn and aerate

Grind

Greatly accelerate

Don’t have a backbone (therefore few humane concerns)

Are hermaphrodites

But:

Don’t like salts

Ammonia above 500ppm

Can’t freeze

Eisenia fetida (or foetida) – red wiggler, manure worm, tiger worm

In classroom:

Scientific method - Food preferences, bin designs

Cafeteria waste

Pre-consumer wastes: prior to being served – best for many reasons

Post-consumer wastes – may cause problems in a school

Vermicomposters:

Bin – Rubbermaid tub with aeration and drain

Stacking trays

Continuous flow reactor

“Worms eat my Garbage” – Mary

Stacking tray system

“Can-O-Worms” – version of stacking system

Continuous flow system:

Worm wigwam system – crank drives a scraper bar

Google:vermitech

Youth Aquaculture Program

Use virtual farm to connect to aquaculture links

State Fair Aquaculture competition (scholarship points awarded)

Targeting youth at risk

Bert Reid

Contact Ron Southwick for a permit.

State Fair contest

Jim McVey

National SeaGrant Program office in DC

Book on Marine Education – Texas SeaGrant

See handouts for Dept. Commerce objectives

-$5,000,000 competition every year.

Ornamentals in the classroom (higher $$ returns)

-Zebrafish

-Nori sushi – pigments (several hundred dollars per pound)

-Ginat clams

-Aquarium fish (live-bearers)

Aquacultre Education in Kentucky

-Gordon J. Mengel

Pat Beachy and Tom Hawthorne - Linganore HS

FFA Engineering!

Bill Shumate and Dennis Blalok – Tunstall HS (Pittsylvania Co.)

DuPont $8,000 grant (did not get Toyota grant)

Tissue cultures with Fralin folks

Partnership with Blue Ridge Aquaculture

½ of greenhouses in the county are dormant because of tobacco settlements

Biological Applications in Agriculture or Technical Biology/Chemistry II

-Must have completed Biology or Algebra I

-No ag pre-requisite

Second year had a 1:1 feed conversion ratio

See CD-ROM for pictures

Exotic Fish Permit through Blue Ridge

Hold and Sell permit was harder because of open lagoon

Strawberries and tomatoes

Put barley straw in a sack and sink it – produces an algae that kills the algae.

$11,700 grant from federal money.

$20,000 start-up total