Attachment D: Protocol Renewal Requests
Protocol 98-07
I. PI:Todd McBride
II. Protocol Title:The role of stretch activated ion channels in the long-term adaptation of striated muscle to active muscle lengthening
III. Submission Date:5/20/14 Submission for renewal
IV. Authorization Date:1998 original authorization
V. Brief Summary of Rationale and Proposed Activities:The protocol is used for research. Current activity includes collecting pilot data for a Muscular Dystrophy Association (MDA) research award. Initial MDA grant was not funded. Data is being collected to support re-submission. The proposal for funding is currently in revision.
Activities During Concluding Authorization Period
VI. Authorized/Actual Number of Animal Subjects:35-42 animals were authorized total per year were originally authorized. No animals were used during the 13-14 AY.
VII. Important Findings or Educational Benefits: The mdx mice clearly show a deficit in adaption to the eccentric contraction protocol. Creatinekinase levels have been measured at significantly higher levels following both and initial and secondary adaptive bout. Work is currently under way to determine the resting membrane potentials in the muscles following a single and adaptive exercise bout. To this point the mdx mice are significantly different. The work is ongoing and the long term significance and application of these measured differences is yet to be determined.
VIII. Presentations and Publications: None this year
Manuscript currently in preparation May 2014
IX. Changes In Existing Knowledge Or Technology Impacting The Rationale Or Potential Benefits Of The Project: No Changes
X. Unanticipated Events With Adverse Consequences For The Animal Subjects: None
Proposed Modifications
XI. Modifications in Rationale: [If the reasoning in support of the protocol activities has changed, describe these changes and the basis for these changes.]
XII. Modifications in Activities: [Describe any proposed changes in activities and the basis for these changes.]
XIII. Modifications in Personnel:[Describe any proposed changes in personnel and the reason for these changes.]
Protocol 01-01
I. PI:Todd McBride
II. Protocol Title:Use of bullfrogs for nerve recordings in lab courses.
III. Submission Date:5/20/14 request for renewal.
IV. Authorization Date:June 2001 original authorization.
V. Brief Summary of Rationale and Proposed Activities:Sciatic nerve removed from double pithed bullfrog to be utilized for nerve recordings in-vitro.
Activities During Concluding Authorization Period
VI. Authorized/Actual Number of Animal Subjects:12-24 authorized. Class was not taught this year.
VII. Important Findings or Educational Benefits: Provides students opportunity to learn advanced lab techniques in physiology.
VIII. Presentations and Publications: None
IX. Changes In Existing Knowledge Or Technology Impacting The Rationale Or Potential Benefits Of The Project: No Changes
X. Unanticipated Events With Adverse Consequences For The Animal Subjects: None
Proposed Modifications
XI. Modifications in Rationale: [If the reasoning in support of the protocol activities has changed, describe these changes and the basis for these changes.]
XII. Modifications in Activities: [Describe any proposed changes in activities and the basis for these changes.]
XIII. Modifications in Personnel:[Describe any proposed changes in personnel and the reason for these changes.]
Protocol 01-04
I. PI:Todd McBride
II. Protocol Title:Measurement of rat metabolism
III. Submission Date:5/20/14 request for renewal
IV. Authorization Date:June 2004 original authorization.
V. Brief Summary of Rationale and Proposed Activities:Resting metabolism of rats recorded in metabolic chamber designed to accommodate rodents and other small animals.
Activities During Concluding Authorization Period
VI. Authorized/Actual Number of Animal Subjects:12-24 authorized, none used. Class was not taught this year.
VII. Important Findings or Educational Benefits: Provides students opportunity to learn advanced lab techniques in physiology.
VIII. Presentations and Publications: None
IX. Changes In Existing Knowledge Or Technology Impacting The Rationale Or Potential Benefits Of The Project: No Changes
X. Unanticipated Events With Adverse Consequences For The Animal Subjects: None
Proposed Modifications
XI. Modifications in Rationale: [If the reasoning in support of the protocol activities has changed, describe these changes and the basis for these changes.]
XII. Modifications in Activities: [Describe any proposed changes in activities and the basis for these changes.]
XIII. Modifications in Personnel:[Describe any proposed changes in personnel and the reason for these changes.]
Protocol 03-02
I. PI:Isabel C. Sumaya
II. Protocol Title:Biological Psychology Laboratory (303L) Protocol number: 03-02
III. Submission Date: June 4, 2014
IV. Authorization Date:[date of original IACUC authorization letter] Feb 08, 2004
V. Brief Summary of Rationale and Proposed Activities:
The primary goal of the Biological Psychology Laboratory(303L) is to expose the student to a variety of experimental paradigms and methodologies used in the field of Biological Psychology. One important paradigm within the field is the use of animal models. The animal paradigm that is used in the 303 laboratory is the Open Field test which is a measure of general spontaneous locomotor activity and exploratory behavior in a novel environment. This paradigm also allows for the observation and measurement of species-common behavior including grooming and rearing. The students use the Open Field test to assess the effects of the light/dark cycle on spontaneous locomotor and species-common behavior as well as exploratory activity in a novel environment.
Activities During Concluding Authorization Period
- Authorized/Actual Number of Animal Subjects:[How many animals were authorized during the concluding authorization period and how many animals were actually “used”/”studied”? Explain any differences.]: 0/11. I did not teach the lab this past year therefore no rats were used. I would like to keep this protocol open as I am scheduled to teach the lab next school year.
- Important Findings or Educational Benefits: [Describe the actual benefits of the activities carried out during the concluding authorization period. For Research protocols present important findings. For Education protocols argue that intended educational benefits were achieved.]
The educational benefits are great. First, the students must successfully complete models I and II of the IACUC on-line training. This exposes the students to the rules and regulations that must be followed when performing animal research and widens their view of the historical perspective of animal research. Students often do not understand the protocols that must be followed in order to do animal research prior to taking the course. Second, students learn first-hand the care that must be taken while doing animal research. They gain an appreciation for the time and commitment that goes into performing animal research. For some, this experience triggers a desire to participate in research on campus in labs that use animal models.
VIII. Presentations and Publications:[List any scholarly presentations or publications based on activities carried out under this protocol.] None
IX. Changes In Existing Knowledge Or Technology Impacting The Rationale Or Potential Benefits Of The Project: [Describe any developments in the field that would alter the arguments for potential benefits put forth in the original protocol.] No changes
X. Unanticipated Events With Adverse Consequences For The Animal Subjects:[Attach a completed IACUC Adverse Event Form for each adverse event.] None
Proposed Modifications
XI. Modifications in Rationale: [If the reasoning in support of the protocol activities has changed, describe these changes and the basis for these changes.] None
XII. Modifications in Activities: [Describe any proposed changes in activities and the basis for these changes.] None
XIII. Modifications in Personnel:[Describe any proposed changes in personnel and the reason for these changes.] None
Protocol 04-01
I. PI:Isabel C. Sumaya
II. Protocol Title:Circadian Effects of Melatonin on Extrapyramidal Side Effects in Rats:
Treated with Typical and Atypical Antipsychotics
Protocol number: 04-01
III. Submission Date: June 4, 2014
IV. Authorization Date:[date of original IACUC authorization letter]? October 03, 2003
V. Brief Summary of Rationale and Proposed Activities:
Antipsychotics are extremely effective in the treatment of schizophrenia, however, a high percentage of those treated with antipsychotics suffer from serious motor-related abnormalities known as extrapyramidal side effects. Previously, the present investigator reported that treatment with the neurohormone, melatonin, substantially reduced antipsychotic-induced extrapyramidal side effects in rats. However, the ameliorating effects of melatonin were investigated in only one sub class of antipsychotic. Therefore, the proposed project will investigate the effect of melatonin on both typical and atypical antipsychotics in rats. Additionally, because the synthesis and secretion of endogenous melatonin follows a clear circadian pattern (nadir during the day, acrophase at night), the effects of melatonin on antipsychotic side effects will be investigated during the light and dark phases of the circadian cycle.
Activities During Concluding Authorization Period
- Authorized/Actual Number of Animal Subjects:[How many animals were authorized during the concluding authorization period and how many animals were actually “used”/”studied”? Explain any differences.]: 144/0
We were not able to complete any work on this project during this past year but want to keep the protocol open.
- Important Findings or Educational Benefits: [Describe the actual benefits of the activities carried out during the concluding authorization period. For Research protocols present important findings. For Education protocols argue that intended educational benefits were achieved.]
Since no data were collected during this period there is nothing to report.
VIII. Presentations and Publications:[List any scholarly presentations or publications based on activities carried out under this protocol.] None
IX. Changes In Existing Knowledge Or Technology Impacting The Rationale Or Potential Benefits Of The Project: [Describe any developments in the field that would alter the arguments for potential benefits put forth in the original protocol.] No changes
X. Unanticipated Events With Adverse Consequences For The Animal Subjects:[Attach a completed IACUC Adverse Event Form for each adverse event.] None
Proposed Modifications
XI. Modifications in Rationale: [If the reasoning in support of the protocol activities has changed, describe these changes and the basis for these changes.] None
XII. Modifications in Activities: [Describe any proposed changes in activities and the basis for these changes.] None
XIII. Modifications in Personnel:[Describe any proposed changes in personnel and the reason for these changes.] Yes, please add to the protocol student, SamirahHussain. She was just chosen a Student Research Scholar and will be working on this project as a part of her SRS research activity. She has completed the necessary IACUC training and has been working in the lab for the last year.
Protocol 04-02
I. PI:Isabel C. Sumaya
II. Protocol Title:The Effects of a High-Fat Diet in an Animal Model of Depression in Rats
Protocol number: 04-02
III. Submission Date: June 6, 2014
IV. Authorization Date:[date of original IACUC authorization letter]? February 8, 2004
V. Brief Summary of Rationale and Proposed Activities:
This protocol focuses on the effects of diet (high-fat) on depression, anxiety and learning. I request that the protocol stay open.
Activities During Concluding Authorization Period
- Authorized/Actual Number of Animal Subjects:[How many animals were authorized during the concluding authorization period and how many animals were actually “used”/”studied”? Explain any differences.]: 60/56. Thirty-seven rats have died of natural causes leaving us with 19 rats from the original 56.
We put the rats on a high fat diet in the Fall and are now measuring the effects on learning and memory. I request that this protocol remain open so that we can continue to collect data.
- Important Findings or Educational Benefits: [Describe the actual benefits of the activities carried out during the concluding authorization period. For Research protocols present important findings. For Education protocols argue that intended educational benefits were achieved.] We have found that high fat diets serve to have a negative impact on learning and memory.
- Presentations and Publications:[List any scholarly presentations or publications based on activities carried out under this protocol.] We presented data at the Society for Neuroscience Meeting this past November in San Diego, Ca.
IX. Changes In Existing Knowledge Or Technology Impacting The Rationale Or Potential Benefits Of The Project: [Describe any developments in the field that would alter the arguments for potential benefits put forth in the original protocol.] No changes
X. Unanticipated Events With Adverse Consequences For The Animal Subjects:[Attach a completed IACUC Adverse Event Form for each adverse event.] None
Proposed Modifications
XI. Modifications in Rationale: [If the reasoning in support of the protocol activities has changed, describe these changes and the basis for these changes.] None
XII. Modifications in Activities: [Describe any proposed changes in activities and the basis for these changes.] None
XIII. Modifications in Personnel:[Describe any proposed changes in personnel and the reason for these changes.] Please add Andrew Suter to this protocol. He was recently chosen a Student Research Scholar (SRS) and this protocol will serve as his project for the2014-2015 school year. He has completed the on-line IACUC training for Modules I and II and has been working in the lab for the past year. He will work directly under my supervision.
Protocol 05-01
I. PI: Carol Raupp (Psychology) Protocol Number: 05-01
II. Protocol Title: Longitudinal naturalistic observation of a trap-neuter-release (TNR)
feral cat colony
III. Submission Date: June 2, 2014
IV. Authorization Date: April 2005 (renewed yearly, most recently 2013)
V. Brief Summary Of Rationale And Proposed Activities: (Same as prior years.) Activities authorized include observation of feral cats during the routine trap-neuter-release (TNR) management of these colonies. Includes collection of data of other species seen in the vicinity of the cats. Rationale: There is little information available about the long-term stability of TNR feral cat colonies or with the inclusion of other species seen in their vicinity.
Activities During Concluding Authorization Period
VI. Authorized/Actual Number Of Animal Subjects: n/a (naturalistic observation of varying numbers)
VII. ImportantFindings Or Educational Benefits: Compared to 2013 the population of feral cats at the CSUB campus has decreased to 10 to 12 (from 12 to 14) after one apparently healthy spayed cat disappeared in December 2013 and another spayed female cat appeared weak but could not be trapped, and then disappeared in Spring 2014. One 4-week-old abandoned kitten was removed from campus in the Fall 2013. No kittens have been born on campus for five years. Sightings of Western striped skunks remain fairly uncommon in the past year (several times a month, across the campus, as I circle the campus each night), and there has been no close cat-skunk proximity observed. An opossum has been seen once near one site. Sightings of San Joaquin kit foxes have been frequent, with another three or four dens with pups on campus in the Spring of 2014. As in the past, there have been no observed instances of physical contact between cats and kit foxes. As in the past, there have been very rare occasions (a handful of times across the year) when a cat has made a short dash toward a nearby kit fox (with the foxes keeping a distance of several feet apart). Because the cats do not adopt an aggressive appearance (puffing up or hissing), this seems more by way of a warning than an attack. At three of the five campus feeding sites, kit foxes have been observed barking at the cats from two to six feet away, and the cats may or may not retreat when this happens.
Off-campus, the number of cats remains stable (about 20) at the five feeding sites formerly in use, although this has included the disappearance of a few cats and the arrival of others. One abandoned cat was removed from one of these sites in Winter 2014. No San Joaquin kit foxes have been seen this year, and a skunk was seen at one site, adjacent to an agricultural field, two times. Free-roaming (“stray”) dogs are seen at these sites occasionally (several times a year).
In the Fall of 2013 I was contacted about an apparently injured cat at a fast-food business on White Lane near Ashe. I added this site to my TNR rounds and observations after finding about 12 cats there, none spayed or neutered, although workers there feed them. The “injured” cathas a severely deformed front leg but manages to cope. I had one severely ill 4-week-old kitten from this site euthanized and removed another, who recovered. Because I have not been able to complete spay-neutering here, four kittens have been born at this site this spring. Rather surprisingly (to me), I have seen a kit fox in the parking lot here twice, possibly from a home base near the RR tracks and industrial sites to the south on District Avenue.