Appendix – Cladistic analysis – data.

Appendix 1. Characters and states selected for analysis.

1. Eye pigmentation; compound eye absent (0); reduced (1); present (2).

2. Head shape; rostrum forms most anterior point in lateral view (0); middle of head most anterior point, not rostrum (1).

3. Rostrum; present (0); absent (1).

4. Head pores; two main pores (0); three main pores, small pores no cosmaria (1); three main pores, small pores with cosmaria (2).

5. Labral keel with elongated apex; present (0); absent (1).

6. Labral keel setule groups; present (0); absent (1).

7. First antenna elongated; present (0); absent (1).

8. First antenna subapical aesthetasc next to sensory seta; present (0); absent (1).

9. First antenna sensory seta; implanted at half to one third of apex (0); terminal (1).

10. First antenna apical aesthetascs; similar length (0); one longer (1) or two longer (2).

11. A2 exopod seta; present, longer than exopod segments (0); present, shorter than exopod segments (1); absent (2).

12. A2 spine on first endopod segment; present and longer than half second segment (0); reduced (1).

13. P1 accessory seta on endite 6 (base of IDL); absent (0); present (1).

14. P1 anterior setae on endites one and two; reduced (0); long (1).

15. P1 dorsal seta on first endite; present (0); reduced (1); absent (2).

16. P2 gnathobasic process; short (0); elongate (1).

17. P2 exopodite seta; present (0); absent (1).

18. P2 soft seta at base of first scraper; present (0); absent (1).

19. P3 number of exopodite setae; six (0); seven (1).

20. P3 length second exopodite seta; long, more than twice other setae (0); similar length (1).

21. P4 endite receptor after last flaming torch seta; marginal (0); on inner side (1).

22. P4 endite inner setae (1’–3’); three long plumose (0); three short naked (1);two reduced one long (2); all absent (3).

23. P4 apex of fifth exopodite seta; acute (0); blunt (1).

24. P4 apex of sixth exopodite seta; acute (0); blunt (1).

25. P4 flaming torch setae; well developed (1); reduced, with distal part missing (1).

26. P5 exopodite margin; concave (0); straight (1); convex (2).

27. P5 filter comb; three setae (0); two setae (1); absent (2).

28. P5 first seta of inner portion; twice as long as inner lobe (0); as long as inner lobe or just longer (1).

29. P6; present (0); absent (1).

30. Postabdomen marginal denticles; merged (0); unmerged (1).

31. Postabdomen dorsodistal gap (between basal claw and dorsodistal margin); open (0); closed (1); absent (2).

Appendix 2. Character data matrix. Data for morphology based on Alonso (1996), Van Damme et al. (2003), Kotov (2004), Kotov & Sanoamuang (2004), this study (Phreatalona) and unpublished data (quadrangularis). Characters 10 and 14 were indicated as parsimony-uninformative by PAUP.

1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18 / 19 / 20 / 21 / 22 / 23 / 24 / 25 / 26 / 27 / 28 / 29 / 30 / 31
Alona quadrangularis / 2 / 0 / 0 / 1 / 1 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 0 / 1 / 0 / 2
Alona affinis / 2 / 0 / 0 / 0 / 1 / 0 / 1 / 1 / 0 / 2 / 1 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 2
Acroperus harpae / 2 / 1 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 1 / 1 / 1 / 1 / 0 / 0 / 1 / 0 / 0 / 1 / 0 / 1 / 1
Nicsmirnovius eximius / 2 / 1 / 0 / 2 / 1 / 1 / 1 / 0 / 0 / 1 / 2 / 0 / 0 / 1 / 2 / 1 / 1 / 1 / 0 / 1 / 1 / 2 / 0 / 1 / 0 / 1 / 2 / 1 / 1 / 1 / 2
Nicsmirnovius greeni / 2 / 1 / 0 / 2 / 1 / 1 / 1 / 0 / 0 / 1 / 2 / 0 / 0 / 1 / 2 / 1 / 1 / 1 / 0 / 1 / 1 / 2 / 0 / 1 / 0 / 1 / 1 / 1 / 1 / 1 / 2
Nicsmirnovius fitzpatricki / 2 / 1 / 0 / 2 / 0 / 1 / 0 / 0 / 0 / 1 / 2 / 0 / 0 / 1 / 2 / 1 / 1 / 1 / 0 / 1 / 1 / 2 / 0 / 1 / 0 / 1 / 1 / 1 / 1 / 1 / 2
Nicsmirnovius camerounensis / 0 / 1 / 0 / 2 / 0 / 1 / 0 / 0 / 0 / 1 / 2 / 0 / 0 / 1 / 2 / 1 / 1 / 1 / 0 / 1 / 1 / 2 / 0 / 1 / 0 / 1 / ? / 1 / 1 / 1 / 2
Phreatalona labrosa / 2 / 1 / 0 / 1 / 0 / 1 / 1 / 1 / 1 / 2 / 1 / 1 / 1 / 1 / 2 / 1 / 0 / 0 / 1 / 1 / 1 / 3 / 0 / 0 / 0 / 1 / 2 / 1 / 1 / 1 / 0
Phreatalona smirnovi / 0&1 / 1 / 1 / 1 / 0 / 1 / 1 / 1 / 1 / 2 / 0 / 1 / 1 / 1 / 2 / 1 / 0 / 0 / 1 / 1 / 1 / 3 / 1 / 1 / 1 / 0 / 2 / 1 / 1 / 1 / 1
Phreatalona protzi / 2 / 1 / 0 / 1 / 0 / 1 / 1 / 1 / 1 / 2 / 0 / 1 / 1 / 1 / 2 / 1 / 0 / 0 / 1 / 1 / 1 / 3 / 1 / 1 / 1 / 0 / 2 / 1 / 1 / 1 / 1
Phreatalona phreatica / 1 / 1 / 1 / 1 / 0 / 1 / 1 / 1 / 1 / 2 / 0 / 1 / 1 / 1 / 2 / 1 / 0 / 0 / 1 / 1 / 1 / 3 / 1 / 1 / 1 / 0 / 2 / 1 / 1 / 1 / 0