supplementary information for

Influence of SILVER NANOPARTICLES on the activity of rat liver mitochondrial ATP-ase

Mariela Chichova*#, MilenaShkodrova*,PenkaVasileva†, KaterinaKirilova*, DilianaDoncheva-Stoimenova*

*Department of Animal and Human Physiology, Faculty of Biology, Sofia University “St. KlimentOhridski”, DraganTzankov Blvd. 8, 1164 Sofia, Bulgaria

†Laboratory of Nanoparticle Science and Technology, Department of General and Inorganic Chemistry, Faculty of Chemistry and Pharmacy, Sofia University “St. KlimentOhridski”, James Bourchier Blvd. 1, 1164 Sofia, Bulgaria

#Corresponding author:; tel: +359 2 8167208; fax:+359 2 865 6641

Fig.S1UV-visible absorption spectra and photographs of starch-stabilized silver nanoparticles (AgNPs/Starch,a) and of raffinose-stabilized silver nanoparticles (AgNPs/Raff,b) recorded after appropriate dilution of the original aqueous dispersion. Spectra recorded immediately and 6 and 62 days after synthesis of AgNPs/Raffare shown;(c) UV-visible absorption spectra ofraffinose- (──) and starch-stabilized (- - -) silver nanoparticles recorded after appropriate dilution of the concentrated by centrifugation aqueous dispersions

The presence of a single narrow and high intensive SRP band (Fig. S1)indicated formation of silver colloids with nanoscale size. The value of λmax(Fig. S1a) was satisfactory and in agreement with the data in the range of 410-422 nm recently published for the synthesis of starch-stabilized nanoparticles without ultrasound irradiation (Chairam and Somsook 2008; Chairam et al. 2009; Cui et al. 2007; Raveendran et al. 2003, 2006;Tai et al. 2008). So far, we failed to found a publication reporting the synthesis of silver nanoparticles with raffinose used both as a reducing and a stabilizing agent. The slight shift of λmax to larger wavelengths and the slight broadening of the absorption band of AgNPs/Raff (Fig. S1b) were comparable to that of AgNPs/Starch and showed larger mean size and a broader size distribution of the raffinose-stabilized nanoparticles versus those stabilized with starch. In addition, no change in the absorbance of AgNPs/Raff was observed 62 days after synthesis (Fig. S1b), indicating high colloidal stability at storage. Our previous experiments manifested high stability of starch-stabilized AgNPs, shown by the lack of alterations in their spectrum obtained after more than one year storage (data not shown).

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