AMJAD FAROOQ PhD
Assistant Professor
Department of Biochemistry & Molecular Biology
University of Miami Miller School of Medicine
Gautier Building, Rm 214
1011 NW 15th Street
Miami, FL 33136
off 305-243-2429 | lab 305-243-9799 | fax 305-243-3955
| http://farooqlab.ws
1. RESEARCH INTERESTS
Understanding the molecular basis of protein interactions at structural, dynamic, thermodynamic and kinetic level, using heteronuclear multi-dimensional NMR in conjunction with other biophysical, biochemical and molecular biological techniques, with particular emphasis on unraveling the molecular mechanisms of breast cancer. Specific areas of interest include:
a. Adaptor Modular Proteins (AMPs)
b. MAP Kinase Phosphatases (MKPs)
c. Cyclin-Dependent Kinases (CDKs)
2. FACULTY APPOINTMENTS
2004-Date Assistant Professor1
Department of Biochemistry & Molecular Biology, University of Miami Miller School of Medicine, Miami, FL 33136
2004-Date Assistant Professor2
Braman Breast Cancer Institute, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136
3. TRAINING RECORD
2002-2004 Instructor3
Structural Biology Program, Department of Physiology & Biophysics, Mount Sinai School of Medicine, New York University, New York, NY 10029
2000-2002 Postdoctoral Associate4
Structural Biology Program, Department of Physiology & Biophysics, Mount Sinai School of Medicine, New York University, New York, NY 10029
1998-2000 Postdoctoral Fellow4,5
Structural Biology Program, Department of Physiology & Biophysics, Mount Sinai School of Medicine, New York University, New York, NY 10029
4. HIGHER EDUCATION
1995-1998 Doctor of Philosophy6
Department of Biochemistry, Imperial College of Science, Technology & Medicine, University of London, London, England, UK
1994-1995 Doctor of Philosophy6
Department of Biochemistry, University of Oxford, Oxford, England, UK
1991-1994 Bachelor of Science7
Department of Biochemistry & Molecular Biology, University of Manchester, Manchester, England, UK
5. AWARDS and HONORS
2003-Date Member of American Association for the Advancement of Science (USA)
2003-Date Member of New York Academy of Science (USA)
1998-2000 Wellcome Trust Postdoctoral Fellowship (UK)
1998 Honorary Diploma from Imperial College London (UK)
1994 First Class Hons Bachelor from University of Manchester (UK)
6. INVITED BOOK CHAPTERS
2. Farooq A, Sudol M & Zhou MM (2005). Two Is Better Than One: Structure, Function and Mechanism of Protein Domains Operating in Tandem Repeats. In Cellular Signaling: New Research, Columbus F ed; Nova Publishing Inc, In Press.
1. Farooq A & Zhou MM (2001). Thermodynamics and Structural Basis of Insulin Receptor Interactions with PTB Domains. In Drug-Receptor Thermodynamics: Introduction and Applications, Raffa RB ed; John Wiley & Sons, Pages 417-431.
7. INVITED REVIEW ARTICLES
2. Farooq A & Zhou MM (2004). PTB or Not to Be: Promiscuous, Tolerant and Bizarro Domains Come of Age. IUBMB Life 56, 547-556.
1. Farooq A & Zhou MM (2004). Structure and Regulation of MAPK Phosphatases. Cellular Signaling 16, 769-779.
8. ORIGINAL RESEARCH PAPERS
21. Qian C, Zhang Q, Wang X, Zeng L, Farooq A & Zhou MM (2005). Structure of the Adaptor Protein p14 Reveals a Profilin-Like Fold With Distinct Function. Journal of Molecular Biology 347, 309-321.
20. Qian C, Zhang Y, Zeng L, Farooq A & Zhou MM (2004). Resonance Assignments for the Endosomal Adaptor Protein p14. Journal of Biomolecular NMR 30, 367-368.
19. Zhang Y, Yan Z, Farooq A, Liu X, Lu C, Zhou MM & He C (2004). Molecular Basis of Distinct Interactions Between Dok1 PTB Domain and Tyrosine-Phosphorylated EGF Receptor. Journal of Molecular Biology 343, 1147-1155.
18. Koch AW, Farooq A, Gruzglin G, Shan W, Zeng L, Colman DR & Zhou MM (2004). Structure of the N-Cadherin Prodomain Reveals a Cadherin Extracellular-Like Domain Fold Without Adhesive Characteristics. Structure 12, 793-805.
17. Koch AW, Farooq A, Zeng L, Colman DR & Zhou MM (2004). 1H, 13C and 15N Resonance Assignments for the N-Cadherin Prodomain. Journal of Biomolecular NMR 28, 87-88.
16. Yan KS, Yan S, Farooq A, Han A, Zeng L & Zhou MM (2003). Structure and Conserved RNA Binding of the PAZ Domain. Nature 426, 468-474.
15. Manzur KL, Farooq A, Zeng L & Zhou MM (2003). 1H, 13C and 15N Resonance Assignments of a Viral SET Domain Histone Lysine Methyltransferase. Journal of Biomolecular NMR 26, 279-280.
14. Farooq A, Zeng L, Yan KS, Ravichandran KS & Zhou MM (2003). Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc. Structure 11, 905-913.
13. Farooq A, Zeng L & Zhou MM (2003). 1H, 13C and 15N Resonance Assignments of the PTB Domain of the Signaling Protein Shc. Journal of Biomolecular NMR 25, 255-256.
12. Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand & Zhou MM (2003). A Dimeric Viral SET Domain Methyltransferase Specific to Lys27 of Histone H3. Nature Structural Biology 10, 187-196.
11. Farooq A, Plotnikova O, Chaturvedi G, Yan S, Zeng L, Zhang Q & Zhou MM (2003). Solution Structure of the MAPK Phosphatase PAC1 Catalytic Domain: Insights into Substrate-Induced Enzymatic Activation of MKP. Structure 11, 155-164.
10. Chaturvedi G, Farooq A, Zeng L & Zhou MM (2003). 1H, 13C and 15N Resonance Assignments of the Catalytic Domain of Human MAPK Phosphatase PAC1. Journal of Biomolecular NMR 25, 79-80.
9. Yan KS, Kuti M, Yan S, Mujtaba S, Farooq A, Goldfarb MP & Zhou MM (2002). FRS2 PTB Domain Conformation Regulates Interactions with Divergent Neurotrophic Receptors. Journal of Biological Chemistry 277, 17088-17094.
8. Mujtaba S, He Y, Zeng L, Farooq A, Carlson JE, Ott M, Verdin E & Zhou MM (2002). Structural Basis of Lysine-Acetylated HIV-1 Tat Recognition by PCAF Bromodomain. Molecular Cell 9, 575-586.
7. Farooq A, Chaturvedi G, Mujtaba S, Plotnikova O, Zeng L, Dhalluin C, Ashton R & Zhou MM (2001). Solution Structure of ERK2-Binding Domain of MKP3: Structural Insights into MKP3 Activation by ERK2. Molecular Cell 7, 387-399.
6. Farooq A, Zeng L & Zhou MM (2001). 1H, 13C and 15N Resonance Assignments of the ERK2-Binding Domain of MAPK Phosphatase MKP3. Journal of Biomolecular NMR 19, 195-196.
5. Farooq A, Plotnikova O, Zeng L & Zhou MM (1999). PTB Domains of Shc and IRS1 Recognize the NPXpY Motif in a Thermodynamically Distinct Manner. Journal of Biological Chemistry 274, 6114-6121.
4. Farooq A (1998). Kinetic Evidence for an obligatory Intermediate in the Folding of the Membrane Protein Bacteriorhodopsin. Biochemistry 37, 15170-15176.
3. Booth PJ & Farooq A (1997). Intermediates in the Assembly of Bacteriorhodopsin Investigated by Time-Resolved Absorption Spectroscopy. European Journal of Biochemistry 246, 674-680.
2. Booth PJ, Riley ML, Flitsch SL, Templer RH, Farooq A, Curran AR, Chadborn N & Wright P (1997). Evidence That Bilayer Bending Rigidity Affects Membrane Protein Folding. Biochemistry 36, 197-203.
1. Booth PJ, Farooq A & Flitsch SL (1996). Retinal Binding During Folding and Assembly of the Membrane Protein Bacteriorhodopsin. Biochemistry 35, 5902-5909.
9. FOOTNOTES
[1]. Tenure-track faculty appointment.
[2]. Secondary faculty appointment.
[3]. Non-tenure-track appointment.
[4]. Postdoctoral training received in Dr Ming-Ming Zhou's laboratory. Postdoctoral research largely focused on structure-function studies of protein modules and enzymes involved in cellular signal transduction using heteronuclear multi-dimensional NMR in conjunction with other biophysical, biochemical and molecular biological techniques.
[5]. Fellowship received from the Wellcome Trust of the United Kingdom.
[6]. First year of doctoral studies was carried out at the University of Oxford. The move to London occurred due to relocation of research adviser. Doctoral studies largely focused on studying the kinetic mechanism of folding of integral membrane proteins using the halobacterial photoactive proton pump bacteriorhodopsin as a prototype and using stop-flow fluorescence and absorption spectroscopy to monitor refolding of bacteriorhodopsin in mixed lipid-detergent bicelles on a millisecond time resolution.
[7]. First Class Honors Bachelor in Biochemistry.
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