Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine
OIE Collaborating Centre Reports
Activities in 2013
Title of Collaborating Centre: / Collaborating Centre forBiotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine
Address of Collaborating Centre: / National Veterinary Institute (SVA)
Swedish University of Agricultural Sciences (SLU)
Uppsala, SWEDEN
Tel.: / +46 18 67 40 00
Fax:
e-mail address: / 1:
2:
3:
website: / www.sva.se/oie-cc
Name of Director of Institute (Responsible Official): / SVA: Dr Jens Mattsson, DG SVA
SLU: Prof Leif Norrgren, Head of Department
Name (including Title and Position) of Head of the Collaborating Centre (formally OIE Contact Point): / Professor Sándor Belák, DVM PhD & DSc
Name (including Title and Position) of writer of this report
(if different from above) / Professor Sándor Belák
Dr Karl Ståhl, Deputy State Epizootologist, SVA
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
ToR: To provide services to the OIE, in particular within the region, in the designated specialty, in support of the implementation of OIE policies and, where required, seek for collaboration with OIE Reference Laboratories
ToR: To identify and maintain existing expertise, in particular within its region
1. Activities as a centre of research, expertise, standardisation and dissemination of techniques within the remit of the mandate given by the OIE
Disease control
Epidemiology, surveillance, risk assessment, modelling
Training, capacity building
Zoonoses
Wildlife
Avian diseases
Aquatic animal diseases
Animal welfare
Diagnosis, biotechnology and laboratory
Veterinary medicinal products
Vaccines
Food safety
Feed safety
Other (Name the category: )
DIAGNOSIS, BIOTECHNOLOGY AND LABORATORYTitle of activity / Scope
Diagnostic activities for Flaviviruses / A total of 2973 equine sera were analysed for the presence of anti-West Nile Virus (WNV) IgM (through a WN IgM capture ELISA), and 623 avian origin samples were screened for the detection of WNV RNA through RT-PCR, in the framework of the surveillance activity for Flaviviruses in the Veneto region, Italy in 2012.
Title of activity / Scope
Research activities in EU project RAPIDIA / Development and evaluation of diagnostic assays, for example, CSFV LAMP-LFD, portable PCR for NDV; testing feces for viral infections in pigs.
Other:
Title of activity / Scope
Research activities in EU projejt EPI-SEQ / High throughput sequencing of a range of veterinary patgogens, such as Newcastle Disease virus, among others.
ToR: To propose or develop methods and procedures that facilitate harmonisation of international standards and guidelines applicable to the designated specialty
2. Proposal or development of any procedure that will facilitate harmonisation of international regulations applicable to the surveillance and control of animal diseases, food safety or animal welfare
–
ToR: To establish and maintain a network with other OIE Collaborating Centres designated for the same specialty, and should the need arise, with Collaborating Centres in other disciplines
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other centres, laboratories or organisations
3. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres (CC), Reference Laboratories (RL), or organisations designated for the same specialty, to coordinate scientific and technical studies?
Yes No
Name of OIE CC/RL/other organisation(s) / Location / Region of networking Centre / PurposeAnses, France
DTU-VET, Denmark
CVI, The Netherlands / Paris / Africa
Americas
Asia and Pacific
Europe
Middle East / New diagnostic tools regarding tick-borne pathogens within the framework of CoVetLab collaboration. A better understanding of their epidemiology, requires multiplex of pathogens in one sample (tick or pool of ticks). Development of low density DNA microarrays that will be used as new epidemiologic investigative tools is ongoing.
AHVLA, UK
Anses, France
CVI The Netherlands / Africa
Americas
Asia and Pacific
Europe
Middle East / Development and validation of new molecular tool for EAV and EIA diagnosis
NADDEC, Kampala, Uganda / Uganda / Africa
Americas
Asia and Pacific
Europe
Middle East / Development of OIE twinning project, accepted in November 2013. Launch May 2014
OIE Collaborating Centre; Joint Animal Health Division of FAO-IAEA, Vienna / Austria / Africa
Americas
Asia and Pacific
Europe
Middle East / CONSULTANT MEETINGS: Advances in Development of Early Warning Tools for Detection of Vector-borne Diseases of Animals, including Zoonoses - Focus on Vectors
and Development of Diagnostic and Tracing Technologies used for Livestock Pathogen Detection”
4. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres, Reference laboratories, or organisations in other disciplines, to coordinate scientific and technical studies?
Yes No
ToR: To place expert consultants at the disposal of the OIE.
5. Did your Collaborating Centre place expert consultants at the disposal of the OIE?
Yes No
Name of expert / Kind of consultancy / SubjectProf. Sándor Belák / Member of OIE ad hoc group on High Throuphput Sequencing / Application of High-throughput Sequencing in Veterinary Infection Biology and Diagnostics
Prof. Sándor Belák et al. / Authors of chapter / High-throughput Sequencing in Veterinary Infection Biology and Diagnostics, OIE Scientific and Technical Review, in press
Dr JF Valarcher / Member of the EUFMD / Expert in Special Committee on Research and Development of the FMD Programme
Dr F Widén et al. / Authors of chapter / Development of improved analytical methods for use in animal health and in foodborne disease surveillance for source attribution: A report from the OIE Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine. OIE Scientific and Technical Review.
ToR: To provide, within the designated specialty, scientific and technical training to personnel from OIE Member Countries
6. Did your Collaborating Centre provide scientific and technical training, within the remit of the mandate given by the OIE, to personnel from OIE Member Countries?
Yes No
If the answer is yes, please provide the total number of trained persons for each of the following categories:
a) Technical visits: 2
b) Seminars:
c) Hands-on training courses:
d) Internships (>1 month): 1
Please provide details (specify the type of training provided using the letter ranked above/overleaf)
Type of technical training provided (a, b, c or d) / Content / Country of origin of the expert(s) provided with training / No. participants from the corresponding countrya / Provided training on a novel method for the detection of Echinoccocus multilocularis / UK / 2
d / Development of a Luminex assay for vesicular swine diseases (FMDV; SVDV; VSV) using novel amplification technologies / Sl / 1
ToR: To organise and participate in scientific meetings and other activities on behalf of the OIE
7. Did your Collaborating Centre organise or participate in the organisation of scientific meetings on behalf of the OIE?
Yes No
National/International / Title of event / Co-organiser / Date (mm/yy) / Location / No. Participants
International / First meeting of the Global African Swine Fever Research Alliance (GARA) / USDA, OIE, FAO / April 2013 / Plum Island, NY / 65
International / Launch of the Global Platform for African Swine Fever and other pig diseases / FAO, OIE, GF-TADS / Nov 2013 / Rome / 60
International / 10th OIE Seminar
New Approaches to Diagnosis: Opportunities and Challenges
to be held during the 16th WAVLD Symposium, Berlin, Germany (5–8June 2013) / WAVLD / June 2013 / Berlin / Ca 300
ToR: To collect, process, analyse, publish and disseminate data and information relevant to the designated specialty
8. Publication and dissemination of any information within the remit of the mandate given by the OIE that may be useful to Member Countries of the OIE
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals: 18
1. Anjum MF, Jones E, Morrison V, Tozzoli R, Morabito S, Toth I, Nagy B, Smith G, Aspán A, Nielsen EM, Fach P, Herrera-León S, Woodward MJ, LA Ragione RM. Use of virulence determinants and seropathotypes to distinguish high- and low-risk Escherichia coli O157 and non-O157 isolates from Europe. Epidemiol Infect. 2013 Jul 18:1-10.2. Anniballi F, Fiore A, Löfström C, Skarin H, Auricchio B, Woudstra C, Bano L, Segerman B, Koene M, Båverud V, Hansen T, Fach P, Tevell Aberg A, Hedeland M, Olsson Engvall E, De Medici D. Management of animal botulism outbreaks: from clinical suspicion to practical countermeasures to prevent or minimize outbreaks. Biosecur Bioterror. 2013 Sep;11 Suppl 1:S191-9. doi: 10.1089/bsp.2012.0089.
3. Anza I, Skarin H, Vidal D, Lindberg A, Båverud V, Mateo R. The same clade of Clostridium botulinum strains is causing avian botulism in southern and northern Europe. Anaerobe. 2014 Jan 10. pii: S1075-9964(14)00003-1. doi: 10.1016/j.anaerobe.2014.01.002.
4. Belák S, Karlsson OE, Blomström AL, Berg M, Granberg F.: New viruses in veterinary medicine, detected by metagenomic approaches. Vet Microbiol. 2013 Feb 1. pii: S0378-1135(13)00074-6. doi: 10.1016/j.vetmic.2013.01.022. [Epub ahead of print]
5. Belák, S., F Karlsson, O. E., Leijon, M. & Granberg, F (2013) High-Throughput Sequencing in Veterinary Infection Biology and Diagnostics. Bulletin of the OIE, 2013-2, 3-7.
6. Belák, S., Karlsson, O. E., Leijon, M. & Granberg, F. (2013) High-throughput sequencing in veterinary infection biology and diagnostics, Rev. sci. tech. Off. int. Epiz., 32 (3) (In press)
7. Belák, S., Karlsson, O.E., Blomström, A-L., Berg, M., Granberg, F.: New viruses in veterinary medicine, detected by metagenomic approaches. Veterinary Microbiology, 2013. 165 (1-2), 95–101
8. Chowdry VK, Luo Y, Widén F, Qiu HJ, Shan H, Belák S, Liu L.: Development of a loop-mediated isothermal amplification assay combined with a lateral flow dipstick for rapid and simple detection of classical swine fever virus in the field. J Virol Methods. 2013 Dec 1;197C:14-18. doi: 10.1016/j.jviromet.2013.11.013. [Epub ahead of print]
9. Granberg, F., Karlsson, O. E., Leijon, M., Liu, L. & Belák, S., Molecular biology approaches to recognize relevant and emerging infectious diseases in animals. In Molecular Diagnosis in Veterinary Laboratory Practice, 2014 (In Press).
10. Granberg, F., Vicente-Rubiano, M., Rubio-Guerri, C., Karlsson, O., Kukielka, D., Belák, S., Sánchez-Vizcaíno, J.M.: Metagenomic Detection of Viral Pathogens in Spanish Honeybees: Co-infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses. PLoS One. 2013;8(2):e57459. doi: 10.1371/journal.pone.0057459. Epub 2013 Feb 27.
11. Karlsson, O.E., Belák, S., and Granberg, F.: The Effect of Preprocessing by Sequence-Independent, Single-Primer Amplification (SISPA) on Metagenomic Detection of Viruses. Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science, 2013. 11(S1), 227-234.
12. Karlsson, O.E., Hansen, T., Knutsson, R., Löfström, C., Granberg, F., Berg, M.: Metagenomic detection methods in biopreparedness outbreak scenarios. Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science, 2013. 11(S1), 146-157.
13. Leijon, M. & Belák, S. (2013) Review of a New Molecular Virus Pathotyping Method for Avian Influenza and Newcastle Disease Viruses. Biosecur. Bioterror., 11, S241-S246
14. Lindahl S, Båverud V, Egenvall A, Aspán A, Pringle J. Comparison of sampling sites and laboratory diagnostic tests for S. equi subsp. equi in horses from confirmed strangles outbreaks. J Vet Intern Med. 2013 May-Jun;27(3):542-7.
15. Widén, F., Leijon, M., Olsson Engvall, E., Muradrasoli, S., Munir, M. & Belák, S. (2013) Development of improved analytical methods for use in animal health and in foodborne disease surveillance for source attribution, Rev. sci. tech. Off. int. Epiz., 32 (2), 549-558.
16. Widgren S, Eriksson E, Aspán A, Emanuelson U, Alenius S, Lindberg A. Environmental sampling for evaluating verotoxigenic Escherichia coli O157: H7 status in dairy cattle herds. J Vet Diagn Invest. 2013 Mar;25(2):189-98
17. Woudstra C, Skarin H, Anniballi F, Auricchio B, De Medici D, Bano L, Drigo I, Hansen T, Löfström C, Hamidjaja R, van Rotterdam BJ, Koene M, Bäyon-Auboyer MH, Buffereau JP, Fach P. Validation of a real-time PCR based method for detection of Clostridium botulinum types C, D and their mosaic variants C-D and D-C in a multicenter collaborative trial. Anaerobe. 2013 Aug;22: 31-7. doi: 10.1016/j.anaerobe.2013.05.002.
18. Ågren J, Hamidjaja RA, Hansen T, Ruuls R, Thierry S, Vigre H, Janse I, Sundström A, Segerman B, Koene M, Löfström C, Van Rotterdam B, Derzelle S. In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences. Virulence. 2013 Sep 4; 5(1).
b) International conference presentations: 9
1. Karlsson, O.E., Norling, M., Granberg, F., Belák, F., Bongcam-Rudloff E.: Viral Metagenomics – New applications for the broad-range detection of viromes in veterinary and public health settings. The Next NGS Challenge Conference: Data Processing and Integration, Valencia, Spain, 14-16 May 2013. Abstract and oral presentation.2. Belák, S.: High Throughput Sequencing in Veterinary Infection Biology and Diagnostics. 10th OIE Seminar on the New Approaches to Diagnosis: Opportunities and Challenges held during the 16th WAVLD Symposium, Berlin, 5–8 June 2013, keynote lecture
3. Belák, S.; Recent trends in viral diagnosis and in molecular epidemiology. Zirve University, EBN Medical School, Kizilhisar Campus, Gaziantep, Turkey, 19 September 2013, Invited speaker
4. Granberg, F., Karlsson, O.E., Blomström, A-L., Berg, M., Belák S.: New viruses in veterinary medicine, detected by metagenomic approaches. XV S.I.Di.L.V. National Congress, Monreale (PA), Italy, 23-25 October 2013. Keynote lecture.
5. Belák, S. New trends in the diagnosis of vector-borne and other infectious diseases. Report from the OIE Collaboarting Centre for the Biotechnology-based Diagnosis of Infectious Disaeses in Veterinary Medicine. “Advances in Development of Early Warning Tools for Detection of Vector-borne Diseases of Animals, including Zoonoses -Development of Diagnostic and Tracing Technologies used for Livestock Pathogen Detection” 4-6 December 2013, IAEA/FAO, Vienna, Austria, keynote lecture
6. LeBlanc, N.: Research activities of the Molecular and Serological Diagnostic Development Group. FAO and National University of Life and Environmental Sciences regional workshop on adapting innovations and technologies for diagnostic purposes to contribute to the increase of livestock production and food safety, Kiev, Ukraine, November 6-7, 2013. (Keynote speaker, presentation on FAO website) )
7. LeBlanc, N., Okoth, E., Mayega, J.F., Masenbe, C., Ståhl, K., Hakhverdyan, M., Bishop, R.: Portable platforms for the detection of African swine fever virus tested in field conditions in East Africa. 14th International Conference of the Association of Institutions for Tropical Veterinary Medicine, AITVM, Johannesberg, South Africa, 25-29 August 2013 (Oral and abstract)
8. Tenje, M., Xia, H., Evander, M., Hammarström, B., Tojo, A., Belák, S., Laurell, T., LeBlanc, N.: Acoustic trapping used for bead incubation in a Luminex-based multiplex biomarker assay. Acoustofluidics 2013. Southampton, UK, 12 – 13 September 2013 (Oral and abstract)
9. Tenje M., LeBlanc N., Evander M., Hammarström B., Xia H., Tojo A., Belák S. and Laurell T., Significantly improved bead recovery of flow cytometry assay by utilising a new technology platform for sample incubation. Cyto2013, San Diego, CA, USA, May 19-22. (Oral and abstract)
c) National conference presentations: 0