Additional file 1: Lottia gigantea acid-soluble matrix proteins

Protein / Accession / Cleaning / Unique peptides / emPAI / Sub-cellular
location
Uncharacterized protein; domain: Cu-Zn superoxide dismutase, fragment / Lotgi1|101611 / A2
B2
C2 / 5
7
6 / 24.1
38.8
157.5 / IC
Similar to pancreatic lipase-related protein; domain: esterase/lipase/thioesterase / Lotgi1|102397 / A2
B2
C2 / 2
-
2 / 0.5
0.5 / EC
Similar to calumenin; domains: multiple EFh / Lotgi1|103470 / A
B
C / 9
6
7 / 2.7
1.7
1.7 / si/tm / EC/IC (ER)
Uncharacterized protein, fragment/similar to M. edulis collagen; 10% Tyr / Lotgi1|104132 / A
B
C / 2
-
- / 1.4 / EC
Similar to gelsolin; shares 1 peptide with Lotgi1|214936 / Lotgi1|105757 / A2
B2
C2 / 5
-
- / 1.4 / IC
Similar to ezrin/radixin/moesin / Lotgi1|106937 / A2
B2
C2 / 19
10
12 / 19.7
2.8
5.8 / IC
Similar to fascin; domains: fascin / Lotgi1|108036 / A2
B2
C2 / 6
-
- / 4.6 / IC
Uncharacterized protein, fragment?; domain: IG_like (c2) / Lotgi1|108898 / A2
B2
C2 / 8
8
7 / 9.0
5.3
3.0 / EC
Similar to intermediate filament protein; shares peptides with Lotgi1|204921 / Lotgi1|109284 / A2
B2
C2 / 14
-
4 / 1.6
0.2 / IC
Similar to pancreatic lipase-related protein; domain: esterase/lipase/thioesterase / Lotgi1|109426 / A2
B2
C2 / 3
2
3 / 1.2
0.7
1.8 / EC
Similar to osteonectin/BM-40/SPARC, overlapping fragments; domains: EFh, KAZAL, / Lotgi1|109908
Lotgi1|176394 (aa1-135) / A2
B2
C2 / 19
13
16 / 74.0
16.8
35.5 / si/tm / EC
Uncharacterized protein/similar to mucin-like protein 1 fragment / Lotgi1|110884 / A2
B2
C / 7
6
3 / 62.1
14.8
3.0 / si / EC
Similar to thiopurine S-methyltransferase; domain: TPMT / Lotgi1|112328 / A
B
C / 2
-
2 / 0.6
0.6 / IC
Similar to follistatin-like protein; domains: EFh, Kazal_FS / Lotgi1|112867 / A
B
C / 3
4
4 / 0.6
1.9
1.2 / EC
Uncharacterized protein/similar to antistasin; domains: antistasin; 15% Cys / Lotgi1|113221 / A2
B2
C2 / 4
-
3 / 2.2
0.8 / si / EC
Uncharacterized protein/similar to 45kDa Calcium-binding protein; domains: EFh / Lotgi1|113682 / A
B
C / 4
-
- / 1.0 / IC (Golgi lumen)
Similar to lysozyme, g-type / Lotgi1|114561 / A2
B2
C2 / 18
22
22 / 2781.6
9999.0
>10,000 / EC
Uncharacterized protein; domain: Dyp-type peroxidase / Lotgi1|114599 / A2
B
C / 6
2
- / 1.1
0.3 / ?
Uncharacterized protein; 6 repeats of ~30aa, starting with MITPE; 14% Pro, 11% Thr / Lotgi1|115147 / A2
B2
C2 / 10
11
8 / 27.5
14.2
14.2 / ?
Uncharacterized protein/similar to epidermal growth factor receptor kinase substrate 8; domains: SH3, PH / Lotgi1|115450 / A2
B2
C2 / 6
-
- / 0.5 / IC
Uncharacterized protein/similar to ceruloplasmin; domains: cupredoxin, multicopper oxidase type 2 / Lotgi1|115607 / A2
B2
C2 / 44
31
36 / 48.0
10.8
24.4 / EC
Similar to legumain; domain:peptidase C13/legumain / Lotgi1|115714 / A2
B2
C2 / 3
-
3 / 0.4
0.4 / si / EC
Uncharacterized protein; domains: Cys-rich secretory protein (SCP), Ves allergen / Lotgi1|116834
Lotgi1|116974 / A
B
C / 2
-
2 / 3.2
1.4 / EC
Similar to phospholipase A2/group 3 secreted PLA2 / Lotgi1|117176 / A
B
C / 2
-
- / 3.6 / EC
Uncharacterized protein/similar to mucin-like protein 1/2 (fragment) / Lotgi1|121410 / A
B
C / 3
-
- / 2.2 / EC
Uncharacterized protein/similar to mucin-like protein 1/2 (fragment) / Lotgi1|121599 / A
B
C / 2
-
- / 1.5 / EC
Similar to transcriptional activator pur-α / Lotgi1|121721 / A
B
C / -
-
2 / 0.3 / IC
Similar to nucleobindin-2; domains: EFh / Lotgi1|121860 / A
B
C / 18
18
18 / 85.6
47.7
47.7 / IC/EC
Similar to ribosomal protein P2 / Lotgi1|121932 / A
B
C / 6
-
2 / 13.7
1.2 / IC
Similar to matrix metalloproteases-21; domain: ZnMc_MMP, hemopexin / Lotgi1|122269 / A2
B2
C2 / 4
4
5 / 0.4
0.4
0.6 / EC
Uncharacterized protein; domain: DUF938 / Lotgi1|123440 / A
B
C / 3
-
- / 0.9 / ?
Similar to Kunitz-type protease inhibitor kalicludein-3/SHPI-1 and -2 / Lotgi1|123902 / A
B
C / 2
-
- / 3.6 / EC
Similar to multicopper oxidase; domains: multicopper oxidase type 1/2 / Lotgi1|124263 / A2
B2
C2 / 9
2
3 / 1.2
0.2
0.3 / ?
Uncharacterized protein; 12% Lys; domain: SOUL/ heme-binding superfamily / Lotgi1|125538 / A
B
C / 3
-
- / 0.3 / ?
Similar to ubiquitin/polyubiquitin / Lotgi1|126004
Lotgi1|234561
Lotgi1|162671
Lotgi1|233138 / A2
B2
C2 / 3
3
4 / 24.1
14.8
24.1 / IC
Similar to semaphorin-2a; domain: semaphorin/CD100 / Lotgi1|126264 / A
B
C / -
-
2 / 0.3 / TM/EC
Similar to Myo/myotrophin; domain: ankyrin / Lotgi1|128209 / A
B
C / 3
-
- / 1.7 / IC
Uncharacterized protein; domain: RHOD (rhodanese) superfamily; 16% Glu; pI 4.7 / Lotgi1|129390 / A2
B2
C / 5
5
5 / 9.0
5.8
9.0 / ?
Similar to thioredoxin-domain containing protein; domains: thioredoxin-related / Lotgi1|131935 / A2
B2
C2 / 15
13
15 / 7.9
4.4
9.0 / IC (ER)
Similar to Kunitz-type protease inhibitor KCP_HALAI 1 / Lotgi1|132911 / A2
B
C / 3
2
3 / 4.6
2.2
4.6 / EC
Similar to dermatopontin 1 / Lotgi1|133595 / A2
B2
C2 / 5
-
4 / 9.0
5.0 / si / EC
Similar to DnaJ/HSP40; domains: DnaJ, DnaJ_C / Lotgi1|138864 / A
B
C / 7
6
7 / 1.4
1.8
1.4 / IC
Similar to BMSP 1; domains: vWFA / Lotgi1|140660 / A2
B2
C2 / 7
7
7 / 30.6
41.2
30.6 / EC
Similar to aldehyde reductase; domains: aldoketo_reductase / Lotgi1|140773 / A2
B2
C2 / 8
-
- / 2.2 / IC
Similar to membrane alanine aminopeptidase; domain: M1_APN_2 / Lotgi1|140786 / A2
B2
C2 / 37
33
32 / 17.7
9.0
7.3 / IC (ER)
Similar to ribosomal protein L27 / Lotgi1|141455 / A
B
C / 3
-
- / 4.6 / IC
Similar to transaldolase / Lotgi1|142681 / A2
B2
C2 / 2
-
- / 0.3 / IC
Uncharacterized protein; 25% Gln, 10% Glu, 17% Pro, 12% Val, 10% Asn, 10% Leu; 6 short repeats:k/qQQPxVELNKQQP; pI 5.2 / Lotgi1|142790 / A
B
C / 5
5
4 / 67.1
45.4
45.4 / ?
Uncharacterized protein; 38% Gln, 11% Leu, 10% Pro; 5 ~70aa repeats containing shorter repeat motifs like NQQQ and KQQQ / Lotgi1|142814 / A2
B2
C2 / 11
11
12 / 3.9
14.4
16.8 / ?
Similar to perlwapin 1; domains: WAP (4x) / Lotgi1|143247 / A2
B
C / 6
6
7 / 3.6
3.6
5.0 / EC
Similar to sulfatase-1 / Lotgi1|143759 / A
B
C / 3
-
- / 0.5 / IC (ER/Golgi)
Similar to gelsolin/villin / Lotgi1|144968 / A2
B2
C2 / 3
-
- / 1.7 / IC
Similar to histone H3 / Lotgi1|176498
etc / A2
B2
C2 / 2
-
3 / 2.2
-
2.2 / IC
Similar to histone H2A / Lotgi1|181153
etc / A2
B2
C2 / 3
3
3 / 3.6
3.6
3.6 / IC
Similar to calmodulin; domains: EFh, recoverin; N-term acetyl-Ala / Lotgi1|148903
Lotgi1|190032
Lotgi1|203998
Lotgi1|216228 / A
B
C / 2
-
2 / 2.2
1.2 / IC
Similar to ribosomal protein S12 / Lotgi1|150191 / A2
B
C / 4
-
- / 6.2 / IC
Similar to DnaJ/HSP40; domains: DnaJ, TPR repeat / Lotgi1|151060 / A
B
C / 3
5
3 / 0.5
1.0
0.5 / IC
Similar to acyl-protein thioesterase; domain: abhydrolase/lysophospholipase_related / Lotgi1|152136 / A
B
C / -
2
2 / 0.6
0.6 / IC
Uncharacterized protein; 20% Gly, 12% Pro / Lotgi1|152688 / A
B
C / 3
2
3 / 99.0
2.2
30.6 / tm / TM
Uncharacterized protein; domains: Sushi/SRC/CCP (complement control protein), PAN / Lotgi1|152699 / A
B
C / 5
4
2 / 3.6
1.8
0.7 / si / EC
Uncharacterized protein; 11% Pro / Lotgi1|152799 / A2
B
C2 / 27
26
28 / 3161.3
718.7
2274.8 / si / EC
Similar to paramyosin fragment, N-term
Similar to paramyosin fragment, C-term / Lotgi1|153233
Lotgi1|153235 / A
B
C
A
B
C / 7
-
5
3
-
4 / 1.7
1.1
3.0
9.0 / IC
Uncharacterized protein; 10% Leu, 11% Ser / Lotgi1|153653 / A
B
C / 2
2
4 / 0.4
0.4
0.9 / ?
Uncharacterized protein; 11% Glu; pI: 4.3; 3 repeats starting at aa 491, 551, and 611 / Lotgi1|154020 / A2
B2
C2 / 27
28
29 / 60.1
77.1
91.1 / si/tm / EC/TM
Uncharacterized protein / Lotgi1|154423 / A2
B2
C2 / 7
2
3 / 25.8
1.7
4.2 / si/tm / EC/TM
Uncharacterized protein / Lotgi1|154424 / A2
B2
C2 / 7
4
4 / 30.6
3.2
2.2 / si / EC
Uncharacterized protein; domain: NTR(netrin)_like superfamily/meteorin / Lotgi1|154590 / A2
B2
C2 / 3
5
6 / 0.5
1.1
1.4 / si/tm / EC
Uncharacterized protein; domain: CBM6(mannanase_like)-CBM35-CBM36_like superfamily / Lotgi1|154713 / A2
B2
C2 / 2
2
2 / 4.6
4.6
4.6 / si/tm / EC/TM
Uncharacterized protein; domain: Tryp_SPc superfamily, trypsin-like serine protease / Lotgi1|155299 / A
B
C / 6
-
2 / 1.6
0.3 / EC
Uncharacterized protein; domains: CLECT, CUB, Sushi/CCP, LDLRA_2, EGF; pI: 4.6 / Lotgi1|156525 / A
B2
C / 11
4
8 / 1.4
0.3
1.0 / tm / TM
Uncharacterized protein; pI 4.7; domains: CCP, Hyalin, Tyrosine-protein kinase ephrin type A/B receptor-like / Lotgi1|156526 / A
B
C / 12
-
5 / 1.5
0.6 / ?
Similar to FAM20C/DMP4 (dentin matrix protein 4) / Lotgi1|156599 / A2
B2
C2 / 14
15
14 / 6.9
7.9
5.3 / EC
Uncharacterized protein; 12% Leu, 12% Lys, pI 9.4 / Lotgi1|156601 / A
B2
C / 3
3
3 / 1.2
1.2
1.2 / si/tm / EC/TM
Uncharacterized protein; domains: Kazal-type protease inhibitor; 10% Leu; pI: 5.5 / Lotgi1|157133 / A
B
C / -
2
- / 1.7 / si / EC
Uncharacterized protein, 13% Val, shares peptide with Lotgi1|157827 / Lotgi1|157680 / A2
B2
C / 3
-
- / 2.7 / si / EC
Uncharacterized protein; 13% Val, 12% Asp, 10% Ala, pI 4.6; domain: EFh / Lotgi1|157689 / A
B
C / 5
5
4 / 30.6
99.0
55.2 / si / EC
Uncharacterized protein; 13% Val, 11% Asp, pI 4.5 / Lotgi1|157690 / A
B2
C / 5
4
4 / 24.1
9.0
14.8 / tm / TM
Uncharacterized protein; 11% Val; shares peptide with Lotgi1|157680 / Lotgi1|157827 / A2
B2
C2 / 6
4
4 / 67.1
9.0
9.0 / si/tm / EC/TM
Similar to cytochrome c oxidase subunit 5A / Lotgi1|157909 / A
B
C / 2
-
- / 0.5 / IC (Mito)
Uncharacterized protein; 11% Pro; Q-rich C-term (aa210-240) / Lotgi1|158113 / A2
B2
C2 / 13
15
15 / 1583.9
5010.9
1583.9 / ?
Uncharacterized protein; 11% Ser, 10% Gln; Q/S-rich motif aa561-640 / Lotgi1|158316 / A2
B2
C2 / 51
55
56 / 159.0
243.2
354.6 / si / EC
Uncharacterized protein; domains: β-ketoacyl synthase, acyl transferase, NAD(P)-binding, β-ketoacyl reductase / Lotgi1|158379 / A2
B2
C2 / 6
-
- / 0.1 / IC
Uncharacterized protein;13% Pro, 12% Ser / Lotgi1|158905 / A2
B
C2 / 18
13
22 / 288.4
57.8
491.4 / ?
Uncharacterized protein; domain: partial phospholipase_A2_3 / Lotgi1|159173 / A
B
C / 5
4
5 / 3.4
2.4
2.4 / tm / TM
Uncharacterized protein / Lotgi1|159264 / A2
B2
C2 / 10
9
4 / 1.2
1.2
0.4 / si/tm / EC/TM
Similar to buccalin; sequence consists mainly of nineteen ~14aa repeats of the type KRGxDxf/yFxGQLG / Lotgi1|159314 / A2
B
C / 15
3
- / 315.3
1.0 / si/tm / EC
Uncharacterized protein; 26% Gln, 13% Leu, 12% Thr, pI 4; starting with aa 156 8x SNLLQQPDa/tTQqLa/tTNeQQQ / Lotgi1|159331 / A
B
C / 2
2
2 / 9.0
9.0
9.0 / si / EC
Uncharacterized protein; domains: FAD-linked oxidase, arabinono-1,4-lactone oxidase / Lotgi1|159383 / A2
B2
C2 / 5
-
- / 0.9 / ?
Uncharacterized protein; domains: SRCR, chitin-binding / Lotgi1|159694 / A2
B
C / 30
9
36 / 0.8
0.2
1.1 / EC/TM
Uncharacterized protein; domains: SEA, 19% Thr, 13% Ser, 10% Ala / Lotgi1|159695 / A
B
C / 4
-
- / 0.7 / tm / EC/TM
Uncharacterized protein / Lotgi1|159735 / A2
B2
C / 6
-
- / 2.5 / ?
Uncharacterized protein; domains: Chitin-binding_2 peritrophin A / Lotgi1|160173 / A2
B2
C2 / 12
12
12 / 358.4
277.3
463.2 / si / EC
Uncharacterized protein; 13% Gln, 11% Gly, 11% Thr / Lotgi1|160356 / A2
B2
C2 / 7
5
4 / 9.0
4.0
3.0 / ?
Similar to Cys-rich secretory protein-2; domains: CAP/allergen V5/Tpx1-related / Lotgi1|160396 / A
B
C2 / -
-
2 / 0.7 / EC
Similar to 84kDa HSP/HSP90A / Lotgi1|161608 / A2
B2
C2 / -
-
3 / 0.2 / IC
Uncharacterized protein; 12% Pro; G/P-rich C-term (aa90-103) / Lotgi1|162562 / A2
B2
C2 / 5
2
4 / 55.2
2.2
16.8 / si / EC
Uncharacterized protein; 14% Ser, 10% Thr / Lotgi1|162861 / A2
B2
C2 / 16
15
16 / 5.0
7.5
7.4 / si/tm, tm / TM
Similar to thioester-containing protein; domains: α2-macroglobulin / Lotgi1|162872 / A2
B2
C2 / 53
47
52 / 9.3
6.8
8.7 / EC
Uncharacterized protein / Lotgi1|163448 / A2
B2
C2 / 17
18
19 / 43.7
49.1
55.2 / si / EC
Uncharacterized protein; domain: EFh, 17% Asp, 16%Ala, pI: 3.8; 12 ~30aa repeats / Lotgi1|163637 / A2
B2
C2 / 23
16
18 / 83.8
18.3
21.8 / si / EC
Uncharacterized protein/similar to endo α-1,4 polygalactosaminidase; domain: DUF297 / Lotgi1|163670 / A2
B2
C2 / 6
4
6 / 3.6
6.7
5.0 / si/tm / EC/TM
Uncharacterized protein; 16% Ser, 11% Thr, 11% Pro;
pI: 4.9 / Lotgi1|164956 / A
B
C / 2
2
3 / 0.9
0.9
1.3 / si / EC
Uncharacterized protein; 11% Asp, pI 3.8; domains: CCP / Lotgi1|164986 / A
B
C / 2
-
- / 2.2 / si / EC