ACROLEIN TEMPLATE SOP

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Standard Operating Procedures for Acrolein
Acrolein is a highly flammable liquid, acutely toxic by oral ingestion, inhalation, and skin absorption. It is also corrosive, a suspectcarcinogen, and a reproductive and mutagenic toxin. Acroleincan be fatal if swallowed or inhaled; and if in contact with skin or eyes, acrolein causes severe skin burns and eye damage.It can be readily absorbed through the skin and its stabilizer, typically hydroquinone, may cause skin sensitization. Vapors may form explosive mixtures in air.Acrolein may react with oxidizers, acids, bases; and may polymerize if not stabilized.
1. Chemicals/Hazards / Chemical name: Acrolein
CAS number: 107-02-8
Routes of exposure: breathing, eye/skin contact with liquid or vapor
How exposure might occur: [ Click here to enter text ]
Target organs: skin, eyes, respiratory system
Signs/symptoms of exposure:burning or irritation of skin/eyes, respiratory irritation, cough, shortness of breath, headache, and nausea. Acrolein has a highly pungent odor.
2. Process / [Describe or attach what is being done withacrolein, including specific laboratory procedures and quantities used.]
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3. Preparation for Use /
  • Purchase the smallest amount of acrolein feasible for a specific task, or purchase acrolein diluted to the concentration for use.
  • Label the bottle when received with the UWlabel, Caution! Peroxide Forming Chemical (label UOW1716).Guidelines for managing peroxide forming chemicals are on the EH&S website, which includes peroxide testing procedures.
  • Provide hazardous chemical and specific SOP training to personnel working with acrolein and any other personnel authorized or required to be in the laboratory or shared space during work with the chemical.
  • Enter acroleininto MyCheminventory, the online UW chemical inventory system. Attach SDS/MSDS in the process. Annually review amount of acrolein stored and update MyChem inventory or confirm amount previously recorded.
  • Determine appropriate cleaning and decontamination method(s) foracrolein, such as cleaning with soap and water or equivalent agent, then rinsing and drying.
  • Purchase or assemble supplies fora spill cleanup kitforacrolein. Ensure the kit is maintained, anticipated users are trained in its use and the kit is readily available in the laboratory.

4. Environmental/
Ventilation Controls / Perform ALL work with acrolein in a certified chemical fume hood or other approved exhausted containment. Test hood to ensure it is functioning properly prior to beginning work.
5. Personal Protective Equipment (PPE) / The following PPE will be worn when working withacrolein:
  • One pair of re-useable butyl or nitrile gloves (minimum 12 mil thickness) or two pairs of disposable nitrile exam gloves (minimum 6 mil thickness each)when working with acrolein liquid, when cleaning up spills and when handling acrolein-containingsolutions.
  • Chemical safety goggles
  • Lab coat or equivalent. Wear long pants or long skirt, and fully closed shoes.
  • If splash is possible, wear face protection such as a face shield, and an impermeable apron with sleeves.
  • Respiratory protection is usually not needed when all work is conducted in a fume hood.If any procedure may pose an exposure hazard it should be eliminated or strictly isolated. If a potential exposure hazard cannot be eliminated, please contact the EH&S Respiratory Protection Program administrator to discuss respiratory protection or to enroll in the program. Program enrollment includes medical evaluation, training and fit testing for an appropriate respirator. Contact EH&S for information or visit the EH&S website.
Change gloves immediately if contaminated, torn, or punctured.
6. Special Handling Procedures & Storage Requirements / HANDLING
EH&S strongly recommends working with acrolein in the lab when there is someone else available to assist with procedures or accidents if needed.
Weighing and Prep
  • For weighing or use, transport acroleinfrom the storage area to the chemical fume hood in a labeled, sealed non-breakable secondary container.Always remove acrolein from its secondary container in a chemical fume hood in order to safely vent any accumulated vapor.
  • Perform all weighing and preparation of acrolein solutions over plastic-backed absorbent pads in a chemical fume hood. Dispose of pads immediately upon contamination and after completion of tasks in a bag labeled as acroleinhazardous waste.
  • Wear one pair of re-useablebutyl or nitrile gloves (minimum 10 mil thickness) or two pairs of disposable nitrile gloves (minimum 6 mil thickness)for all procedures involving preparation and handling ofacrolein.
  • Change gloves after each use, or immediately when torn, punctured, or contaminated. Wash hands thoroughly. Wash re-usable gloves after use, even if not visibly contaminated.
[Describe how acrolein will be weighed and prepared.]
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Use
  • Only use in designated areas (specify specific fume hood, etc.). Store and/or label acrolein so it will only be handled by those trained to use it.
  • Do not work with acrolein near sources of flame or ignition.
  • If applicable, a sharps container specifically for acroleinwill be in the immediate vicinity for safe sharps disposal.Avoid use of needles/sharps or use safety sharps that you are experienced with when possible.
  • Place all contaminated disposable items in a separate acroleinhazardous waste bag orcontainer before disposal.
  • Re-usable utensils, glassware, and other surfaces contaminated with acrolein must be decontaminated at the end of the laboratory work session. Complete this inside a fume hood before removing any of the items.
  • Clean all contaminated surfaces with soap and water or equivalent agent, then rinse and dry.
  • When work completed, remove gloves and wash hands with soap and water.
STORAGE
  • Store acroleinunder an inert gas in tightly sealed labeled container in a refrigerator designed and approved for storage of flammables. A storage temperature of 2 to 8°C is recommended.
  • Keep away from heat, light,air, flames and sources of ignition.
  • Keep segregated from oxidizers, acids and bases.
  • Check for peroxides periodically. Guidelines for managing peroxide forming chemicals including peroxide testing proceduresare on the EH&S website.
  • Store acroleinin labeled, sealed non-breakable secondary container within the storage area, if potential for disturbance or breakage exists.
TRANSPORT
  • Transport acrolein in labeled and sealed non-breakable secondary containers.

7. Spill and Accident Procedures
[Specific cleaning and waste disposal procedures must be determined.] / Any acroleinspills must be cleaned up immediately by properly protected and trained personnel. All other persons should leave the area. Spill response procedures must be developed. Clean up spills using contents of the spill kit. Do not attempt to clean up any spill if not trained or comfortable. If the spill is out of control, call 911. If a person is injured, exposed or suspected of being exposed to acrolein, call 911 immediately. Follow EXPOSURE PROCEDURES in section 8 below.
Spillsinside a fume hood or approved containment
  1. Close hood sash, cordon off area.
  2. Call EH&S (during business hours (M-F/8-5) 206-543-0467, outside business hours 911); tell them that anacroleinspill has occurredand you need advice or assistance. Notify supervisor.
  3. Personnel must wear a lab coat or smock, safety goggles, and one pair of re-usable butyl or nitrile gloves (minimum 12 mil thickness), Silver Shield gloves, ortwo pairs of disposable nitrile gloves, when cleaning up spills.
  4. Wipe up spilled liquids with absorbent pads.
  5. Clean the spill area thoroughly with soap and water or equivalent agent, then rinse and dry.
  6. If spill is extensive within the containment, clean all interior surfaces after completion of the spill cleanup.
  7. Double bag all waste in plastic bags labeled with the contents and store in fume hood away from incompatible chemicals or procedures. Submit request to EH&S for hazardous waste pick up.
Spills outside of fume hood or approved containment
  1. If there is a risk of fireand/or risk of exposure to acroleinliquid or vapor:
  2. Evacuate the room and pull the fire alarm. Try to turn off all sources of flame and ignition.
  3. As soon as possible report the spill by notifying EH&S (during business hours (M-F/8-5) 206-543-0467, outside business hours 911); tell them that an acrolein spill has occurred and the fire alarm was activated. Notify supervisor.
  4. If there is no risk of exposure or fire:
  5. Evacuate the room and secure access.
  6. As soon as possible report the spill by notifying EH&S (during business hours (M-F/8-5) 206-543-0467, outside business hours 911); tell them that an acrolein spill has occurred, and you need help managing the spill. EH&S will contact a spill cleanup contractor. Notify supervisor.
  7. Be prepared to provide the following information:
  • Name and phone number of knowledgeable person that can be contacted
  • Name of chemical spilled, concentration and amount spilled
  • Number of injured, if any (refer below to EXPOSURE PROCEDURES in case of emergency)
  • Location of spill
This information should also be reported to the Emergency Department (ED) after potential exposure.
Any spill incident requires the involved person or supervisor to complete and submit the Online Accident Reporting System (OARS) form to EH&S within 24 hours (8 hours if serious injury or hospitalization).
For questions on spill cleanup, contact EH&S spill consultants at 206-543-0467.
8. EXPOSURE PROCEDURES
In Case of Emergency /
  1. Provide First Aid Immediately
  • For inhalation exposure, without contaminating yourself, move the individual out of contaminated area to fresh air while awaiting emergency responders. Do not give mouth-to-mouth or mouth-to-nose resuscitation, as this may cause exposure to the rescuer.
  • For sharps injury (needlestick or subcutaneous exposure), scrub exposed area thoroughly for 15 minutes using warm water and sudsing soap.
  • For skin exposure, use the nearest safety shower for 15 minutes. Stay under the shower and remove clothing. Use a clean lab coat or spare clothing for cover-up.
  • For eye exposure, use the eye wash for 15 minutes while holding eyelids open.
  • Wear gloves when assisting a victim.
  1. Get Help
  • Call 911 or go to nearest Emergency Department (ED).Give details of exposure:
  • Chemical
  • Dose
  • Route of exposure
  • Time since exposure
  • Bring to the ED the SDS/MSDS and this SOP
  • Notify your supervisor as soon as possible for assistance.
  • Secure area before leaving. Lock doors and indicate spill if needed.
  1. Report Incident to Environmental Health & Safety
  • Notify EH&S immediately after providing first aid and/or getting help.
  • During business hours (M-F/8-5) call 206-543-7262.
  • Outside business hours call 206-685-UWPD (8973) to be routed to EH&S staff on call.
  • For all incidents and near misses, the involved person or supervisor completes and submits the UW Online Accident Reporting System (OARS) form to EH&S within 24 hours (8 hours if serious injury or hospitalization).

9. Waste Disposal and Cleaning / Contact EH&S Environmental Programs at 206-616-5835 for assistance with hazardous chemical waste disposal procedures.
  • Work space surfaces must be cleaned after completion of workwith soap and water or equivalent agent, then rinsed and dried.
  • Note that acrolein is an Environmental Protection Agency (EPA) P-Listed (acutely toxic) chemical, which has stringent requirements for waste disposal as listed below.
  • Unused and wasteacrolein pure liquid and solutions must be sealedin labeled chemically compatible sturdy containers (no more than one quart in volume) for pickup as hazardous acroleinwaste by EH&S.
  • The bottles acrolein wasreceived in, even when empty, must be managed and collected as hazardous waste. This also includes empty vials, syringes, pipette tips, and other containers if the acroleinwas the sole active ingredient in the container.
  • Used and contaminated absorbent pads, PPE, etc. will be placed in a labeled hazardous chemical waste bag specifically for acrolein waste. Submit request to EH&S for pickup.
  • Contact EH&S about containers and the collection of used, non-biohazardous sharps that are contaminated with acrolein. These items are a hazardous waste and should not be placed in a red sharps container.
  • Keep acrolein waste materials in closed and labeled dedicated bags and containers within a fume hoodaway from incompatible chemicals or procedures before EH&S pickup.
For chemical waste pickup: Complete either an Online Chemical Waste Collection Requestor a Chemical Waste Collection Request Form on the EH&S website.Scan, fax or mail the form to: , FAX to 206-685-2915, or mail to Box 354110.
10. Special Precautions for Use of Acrolein in Animals
(if applicable) / Use of acroleinin animals will be documented and approved by IACUC.
[Give detailed procedures for safely completing tasks and any special disposal requirements.]
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Particularly hazardous
substance involved? / X YES: / Blocks #11to #13are Mandatory
NO: / Blocks #11to #13are Optional.
11. Approval Required / All staff working with acroleinor having access to it must be trained on this SOP prior to starting work. They must also review theacroleinSDS/MSDS, and it must be readily available in the laboratory. All training must be documented and maintained by the PI or their designee.
12. Decontamination / All surfaces and re-useable equipment must be decontaminated with soap and water or equivalent agent, then rinsed and dried.
13. Designated Areas / All work withacroleinmust be done in a designated laboratory and designated chemical fume hood. This work will be conducted in [room #].Theacroleinchemical will be stored ina refrigerator in [room #].
Name:
(PI or Lab Manager) / Title:
Signature: / Date:
[Laboratory Name]
Documentation of Training
Standard Operating Procedure forAcrolein
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EH&S Research and Occupational SafetyPage 1

February2016