Product Specification Document

Thank you for considering licensing your antibody or hybridoma to Abcam. Please take a few minutes to fill out this document, providing all of the information that we need in order to continue our discussions. This document will facilitate an evaluation of the commercial potential of your product, ensure that our Laboratory and Technical Support departments can support your product as a commercial reagent, and will help to determine the maximum royalties we can offer.

Please add your answers, where applicable, to the Word document and email the completed documentas an attachment rnatively, please fax the completed document to +44 (0)1223 696 001.

Thank you for your time,

Abcam Licensing Team.

Date:

Name of Investigator:

Institution:

Department:

Antibody name:

Alternative names:

Entrez Gene code:

Clonality:

Host species:

Clone number:

Will you be providing bulk stock of finished antibody or hybridoma?

Regarding the antibody:

  1. What protein(s)/molecules(s) does the antibody recognise?
  1. Please state the immunogen type used to raise the antibody? E.g. peptide/recombinant protein and the species it was derived from.
  1. Is the immunogen available for QC testing?
  1. What method of purification was used for the finished product (if any)?
  1. If purified, what is the concentration of the antibody and what buffer is it stored in?
  1. Have any preservatives added to the antibody preparation? E.g. sodium azide
  1. At what temperature do you recommend storing the antibody?
  1. What species will the antibody cross react with?
  1. How has the antibody been tested? Please provide protocol details where possible.

Application / Tested (Y/N) / Cell/tissue type used / Rating (good, OK, didn’t work) / Working dilution / Other notes
Western Blot
Immunocytochemistry/
immunofluorescence
Immunohistochemistry
(please specify conditions)
ELISA
Immunoprecipitation (IP)
Chromatin IP
Other (please specify)
  1. Please attach images (e.g. Western blot) demonstrating the specificity of the antibody, along with image legends.
  1. What publications has the antibody been used in? Please list.

Regarding the hybridoma:

  1. Hybridoma type:
  1. How many clonings have been carried out?
  1. Has the cell line been tested for mycoplasma recently? Please state the result.
  1. Please state the immunoglobulin isotype:
  1. Do the cells have normal viability after recovery in culture?
  1. What is the preferred freezing medium composition?
  1. What is the preferred cell culture medium, including supplements? Please also provide any additional information (i.e. are the cells weaned into serum free media?).
  1. What culture system is presently used, ie. Flasks, roller bottle?
  1. Please provide information on the recommended growth temperature, % CO2, doubling time, seed density etc.
  1. Are you able to provide lysate samples etc for testing the antibody?

Notes:

Please enter any further information that may be useful here: