ESM 1: Characteristics and qPCR results of 135 stain hairs. The SNP electropherogramm quality values indicate results where no SNPs (“0”), sporadic SNPs (“1”), a partial profile with haplogroup (“2”) and a full profile (“3”) were obtained.

no. / length / color / root / qPCR [n] / SNP quality
1 / 2.5 / dark brown / no / 8,450 / 1
2 / 2.5 / dark brown / no / 0 / 0
3 / 3 / dark brown / no / 192,000 / 1
4 / 1 / dark brown / no / 0 / 0
5 / 5 / dark brown / no / 145,000 / 3
6 / 1.5 / grey / no / 270,000 / 1
7 / 5 / dark brown / no / 1,540,000 / 3
8 / 6 / dark brown / no / 139,000 / 2
9 / 5 / dark brown / no / 293,000 / 3
10 / 5 / grey / no / 565,000 / 3
11 / 1.5 / light brown / no / 25,500 / 1
12 / 1.8 / brown / no / 0 / 0
13 / 2 / dark brown / no / 0 / 0
14 / 2.5 / grey / no / 9,360 / 1
15 / 0.5 / dark brown / no / 15,200 / 1
16 / 1 / dark brown / no / 136,000 / 1
17 / 2 / dark brown / no / 610,000 / 2
18 / 1 / light brown / no / 163,000 / 1
19 / 1.5 / brown / no / 220,000 / 2
20 / 6 / dark brown / no / 440,000 / 3
21 / 1.5 / blond / yes / 123,000 / 2
22 / 2.5 / dark brown / no / 488,000 / 3
23 / 5 / light brown / no / 574,000 / 3
24 / 2.5 / brown / no / 94,300 / 1
25 / 2.5 / dark brown / no / 26,100 / 2
26 / 4.5 / red-brown / no / 0 / 1
27 / 6.5 / red-brown / no / 535,000 / 3
28 / 2 / light brown / no / 5,850 / 2
29 / 2.5 / dark brown / yes / 508,000 / 3
30 / 2 / dark brown / yes / 90,300 / 3
31 / 2 / dark brown / yes / 2,420 / 2
32 / 3 / dark brown / yes / 3,250 / 2
33 / 1.5 / dark brown / no / 269,000 / 3
34 / 6 / dark brown / yes / 530,000 / 3
35 / 2.5 / dark brown / yes / 224,000 / 3
36 / 2 / dark brown / no / 301,000 / 3
37 / 2 / grey / yes / 9,650 / 3
38 / 1.5 / dark brown / yes / 438,000 / 3
39 / 2 / dark brown / no / 252,000 / 3
40 / 1.5 / brown / yes / 921,000 / 3
41 / 2 / brown / no / 742,000 / 3
42 / 1.5 / brown / yes / 955,000 / 3
43 / 2 / brown / no / 538,000 / 3
44 / 1.5 / brown / yes / 11,800 / 3
45 / 3.5 / dark brown / yes / 1,160,000 / 3
46 / 1.5 / brown / no / 81,800 / 3
47 / 1.5 / brown / yes / 428,000 / 3
48 / 2 / brown / yes / 1,620,000 / 3
49 / 3.5 / light brown / no / 190,000 / 3
50 / 7 / dark brown / no / 573,000 / 3
51 / 4.5 / brown / no / 763,000 / 3
52 / 2.5 / brown / no / 266,000 / 3
53 / 2.5 / brown / no / 152,000 / 3
54 / 2.5 / blond / yes / 75,000 / 3
55 / 3 / dark brown / yes / 201,000 / 3
56 / 1 / dark brown / no / 66,700 / 3
57 / 3.5 / brown / no / 152,000 / 3
58 / 3.5 / brown / no / 328,000 / 3
59 / 1.5 / dark brown / no / 3,840 / 3
60 / 6 / brown / no / 56,600 / 3
61 / 3.5 / brown / no / 137,000 / 3
62 / 3.5 / brown / no / 48,600 / 3
63 / 3 / brown / no / 261,000 / 3
64 / 3 / brown / no / 281,000 / 3
65 / 1.5 / brown / yes / 1,180 / 3
66 / 5.5 / brown / no / 813,000 / 3
67 / 2 / dark brown / no / 168,000 / 3
68 / 2.5 / brown / no / 90,300 / 3
69 / 1.5 / dark brown / no / 8,280 / 3
70 / 1.5 / blond / yes / 6,060 / 3
71 / 3 / dark brown / no / 396,000 / 3
72 / 2.5 / brown / no / 98,000 / 3
73 / 2.5 / dark brown / yes / 177,000 / 3
74 / 1.5 / dark brown / no / 91,600 / 3
75 / 1.5 / dark brown / yes / 25,900 / 3
76 / 0.5 / red-brown / no / 27,000 / 3
77 / 1.5 / red-brown / no / 38,700 / 3
78 / 2 / blond / yes / 6,770 / 3
79 / 3.5 / light brown / yes / 448,000 / 3
80 / 2 / grey / no / 4,390 / 2
81 / 2.5 / blond / yes / 104,000 / 3
82 / 1.5 / blond / yes / 65,300 / 3
83 / 1 / light brown / yes / 83,600 / 2
84 / 1.5 / brown / yes / 723 / 2
85 / 1.5 / blond / yes / 14,900 / 2
86 / 2 / dark brown / yes / 0 / 0
87 / 3.5 / brown / no / 698 / 1
88 / 1 / light brown / yes / 0 / 1
89 / 0.75 / light brown / yes / 9,770 / 1
90 / 2 / blond / yes / 5,860,000 / 3
91 / 1.5 / light brown / yes / 437,000 / 3
92 / 2.5 / light brown / yes / 504,000 / 3
93 / 2 / light brown / yes / 5,430,000 / 3
94 / 1.5 / blond / yes / 0 / 2
95 / 4.5 / dark brown / yes / 138,000 / 3
96 / 4 / dark brown / no / 951,000 / 3
97 / 1.5 / blond / yes / 169,500 / 2
98 / 1.5 / dark brown / no / 68,600 / 1
99 / 4 / blond / no / 349,000 / 2
100 / 2 / blond / yes / 0 / 2
101 / 2.5 / light brown / yes / 0 / 0
102 / 3 / light brown / yes / 0 / 0
103 / 3 / light brown / yes / 0 / 0
104 / 2 / light brown / yes / 0 / 0
105 / 1.5 / light brown / yes / 0 / 1
106 / 2 / blond / yes / 0 / 1
107 / 3.5 / light brown / yes / 377,000 / 3
108 / 2 / brown / yes / 377,500 / 3
109 / 2 / blond / yes / 297,500 / 3
110 / 1.5 / grey / yes / 2,200 / 1
111 / 2 / blond / yes / 128,800 / 3
112 / 4.5 / blond / no / 141,500 / 2
113 / 1.5 / light brown / no / 0 / 1
114 / 1 / brown / yes / 2,610 / 2
115 / 4 / brown / yes / 46,650 / 3
116 / 1 / red-brown / no / 1,870 / 1
117 / 6.5 / dark brown / no / 114,000 / 3
118 / 1.5 / brown / no / 0 / 1
119 / 0.75 / dark brown / no / 0 / 1
120 / 5 / dark brown / no / 0 / 0
121 / 1.5 / dark brown / no / 231 / 2
122 / 8 / light brown / yes / 2.060 / 2
123 / 6.5 / dark brown / yes / 71,400 / 3
124 / 3.5 / blond / yes / 0 / 2
125 / 8.5 / blond / yes / 6,420 / 3
126 / 4.5 / blond / yes / 297 / 2
127 / 4 / brown / yes / 20,400 / 2
128 / 6.5 / light brown / yes / 532 / 3
129 / 2.5 / blond / yes / 44,200 / 3
130 / 2 / brown / yes / 0 / 1
131 / 1.5 / light brown / no / 0 / 1
132 / 1 / dark brown / no / 0 / 0
133 / 1.5 / blond / no / 0 / 0
134 / 1.5 / grey / yes / 42 / 2
135 / 1.5 / blond / yes / 0 / 2

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